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All Journal Damianus Journal of Medicine Dental Journal (Majalah Kedokteran Gigi)
Larope, Ceny Gloria
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Dentistry Health Professions Medicine & Pharmacology Public Health

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The Kemampuan Ekstrak Etanol Daun Binahong (Anredera cordifolia (Ten.) Steenis) Dalam Menghambat Pembentukan Biofilm Streptococcus mutans Hutomo, Suryani; Larope, Ceny Gloria
Bahasa Indonesia Vol 24 No 1 (2025): Damianus Journal of Medicine
Publisher : Atma Jaya Catholic University of Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25170/djm.v24i1.6128

Abstract

Pendahuluan: Streptococcus mutans merupakan bakteri predominan pada karies gigi. Bakteri ini mampu membentuk biofilm. Adanya resistensi antibiotik dan biofilm yang menghambat penetrasinya menyebabkan perlunya dilakukan penelitian untuk mengendalikan terbentuknya biofilm. Binahong (Anredera cordifolia) memiliki aktivitas antibakteri dan mudah ditemukan di lingkungan. Tujuan dari penelitian ini adalah untuk menguji efek antibiofilm ekstrak daun binahong (Anredera cordifolia (Ten.) Steenis) terhadap S. mutans sebagai penyebab utama karies gigi. Metode: Ekstrak dipersiapkan menggunakan metode macerasi. Uji antibakteri S. mutans ATCC 25175 menggunakan metode broth microdilution. Pengujian antibiofilm menggunakan metode yang serupa. Biofilm yang terbentuk diwarnai dengan 0,1% crystal violet dan dibaca pada 595 nm menggunakan microplate reader. Hasil: Hasil uji antibakteri menunjukkan bahwa nilai hambat minimum (MIC) ada pada konsentrasi 3750 µg/ml, sedangkan konsentrasi ekstrak sebagai antibiofilm didapatkan mulai dari konsentrasi 187,5 µg/ml sampai dengan 3000 µg/ml. Analisis statistik menggunakan One-way ANOVA menunjukkan perbedaan yang bermakna dari masing-masing kelompok perlakuan (p=0,000). Simpulan: Ekstrak etanol daun binahong dapat menghambat pertumbuhan S. mutans dengan konsentrasi hambat minimum 3750 µg/ml serta menghambat pembentukan biofilm S. mutans pada konsentrasi efektif 750 µg/ml.
Antibacterial and anti-adherence effect of Laportea interupta ethanolic extract on Streptococcus mutans biofilm Hutomo, Suryani; Larope, Ceny Gloria; Putri, Denise Utami; Sooai, Christiane Marlene; Kristiyanto, Haryo Dimasto
Dental Journal (Majalah Kedokteran Gigi) Vol. 58 No. 3 (2025): September
Publisher : Faculty of Dental Medicine, Universitas Airlangga https://fkg.unair.ac.id/en

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/j.djmkg.v58.i3.p256-261

Abstract

Background: Due to its capacity to generate extracellular polymeric substances that aid in biofilm creation, Streptococcus mutans is a leading cause of dental caries. Natural remedies, including traditional plant extracts, are being explored for their antimicrobial potential. Laportea interupta (L. interupta), known as jelatang in Indonesia, is alleged to possess a bacteria-inhibiting effect. Purpose: The purpose of this study is to evaluate the potential use of L. interupta leaf ethanolic extract to inhibit S. mutans proliferation and adherence to hydroxyapatite (HA) discs, simulating the tooth surface. Methods: The broth microdilution approach was utilized to evaluate the minimum concentration of the extract that inhibits bacterial growth. For anti-adherence assessment, HA discs pre-coated with saliva were exposed to different extract concentrations and incubated with S. mutans. Bacterial adhesion was visualized using 0.1% crystal violet staining, quantified spectrophotometrically at 595 nm, and further verified using scanning electron microscopy (SEM). Results: The extract showed inhibitory effects on S. mutans growth at 7,500 μg/ml (MIC). Anti-adherence activity was optimal at 1,500 μg/ml, and SEM analysis confirmed a reduced biofilm formation on extract-treated samples. Conclusion: Laportea interupta ethanolic leaf extract demonstrated both antibacterial and anti-adherence effects against S. mutans, suggesting its potential as a natural anti-biofilm agent for oral health applications.
Co-Authors Christiane Marlene Sooai, Christiane Marlene Denise Utami Putri, Denise Utami Kristiyanto, Haryo Dimasto Suryani Hutomo, Suryani