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All Journal Journal of Dentistry Indonesia Damianus Journal of Medicine Majalah Kedokteran Gigi Indonesia Dental Journal (Majalah Kedokteran Gigi) Jurnal Atma Inovasia
Suryani Hutomo, Suryani
Fakultas Kedokteran Universitas Kristen Duta Wacana
Humanities Decision Sciences, Operations Research & Management Dentistry Environmental Science Health Professions Medicine & Pharmacology Public Health Social Sciences Other

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Ekspresi Caspase-3 pada Sel Epitel Rongga Mulut (Kb Cell Line) setelah Paparan Ekstrak Kopi Hutomo, Suryani; Suryanto, Yanti Ivana; Susilowati, Heni; Rudolf Phym, Agustinus; Maheswara, Devi Chretella
Majalah Kedokteran Gigi Indonesia Vol 21, No 2 (2014)
Publisher : Majalah Kedokteran Gigi Indonesia

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Abstract

Kopi adalah minuman yang biasa dikonsumsi oleh masyarakat sehari-hari. Telah diketahui bahwa kopi mengandung kafein seperti yang terdapat juga pada teh dan coklat. Kandungan terbanyak kafein terdapat pada kopi. Kafein mempunyai struktur kimia 1, 3, 7- trimethylxanthine dan merupakan derivat xanthine. Senyawa ini dapat menginduksi kematian sel yang mengarah pada apoptosis, namun mekanisme yang terlibat belum diketahui dengan jelas. Tingginya konsumsi kopi di dunia yang selalu meningkat mengindikasikan perlunya dilakukan penelitian untuk mengetahui efek kafein pada epitel rongga mulut yang berkontak langsung dengan kafein. Penelitian terdahulu melaporkan bahwa ekstrak kopi menyebabkan kerusakan sel yang sebagian besar mengarah pada apoptosis, tetapi mekanismenya belum jelas. Tujuan penelitian ini adalah untuk menganalisis mekanisme kematian sel KB yang diinduksi oleh kafein melalui aktivasi caspase-3. Sel KB sebagai model epitel oral (5x10⁴sel) dikultur dalam DMEM menggunakan 24 wells microplate selama 24 jam sebelum perlakuan. Sel selanjutnya dipapar dengan kafein dengan konsentrasi 100 µg/ml, 200 µg/ml, 400 µg/ml dan diinkubasi selama 24 dan 48 jam dalam DMEM. Doxorubicin (0,5625 µg/ml) digunakan sebagai kontrol positif induksi apoptosis. Teknik imunositokimia terhadap caspase-3 dilakukan pada sel setelah dipapar kafein untuk mengamati adanya ekspresi caspase-3 sebagai ciri apoptosis. Identifikasi caspase-3 dilakukan menggunakan mikroskop fase kontras. Ekspresi protein caspase-3 terdeteksi pada sitoplasma sel KB. Hasil penelitian ini menunjukkan adanya ekspresi caspase-3 aktif yang ditandai dengan warna cokelat dengan intensitas kuat pada sitoplasma sebagian besar sel setelah dipapar kafein dengan konsentrasi 100 μg/ml dan 200 µg/ml selama 24 jam. Disimpulkan bahwa ekstrak kopi menyebabkan apoptosis sel KB melalui jalur aktivasi caspase-3. ABSTRACT: The Expression of Caspase-3 in Oral Cavity (Kb Cell Line) after Exposure to Coffee Extract. People widely consume coffee in daily meals. It is known there is caffeine found in coffee like it is found in tea and chocolate. Caffeine is found in the greatest amount of coffee. This 1, 3, 7- trimethyl xanthine substance is a derivate of xanthine that is consumed by almost all people in the world. This substance could induce cell death that mainly is apoptosis, but how the mechanism has not been clearly understood. Considering that coffee is widely consumed in the whole world, it is necessary to conduct an experiment to find any possible effect of caffeine to oral epitel that make direct exposure to caffeine. This experiment is targeted to analyze the mechanism of cell death which caused by caffeine through activation of caspase-3. KB cells as oral epithelial model (5x1044 sel) were cultured in DMEM using 24 well microplate for 24 hours before treatment. Then caffeine was given with concentration of 100 µg/ml, 200 µg/ml and 400 µg/ml. Cells were then incubated for 24 and 48 hours period in DMEM. Doxorubicin (0,5625 µg/ml) was used as a positive control of apoptosis induction. Immunocytochemistry technique was then done to observe any caspase three expression as a marker for apoptosis. Identification of active caspase-3 was then done using contrast phase microscope. The results showed expression of caspase-3 in KB cells cytoplasm which observed as high intensity of brown colored molecules in cell cytoplasm after 100 μg/ml and 200 µg/ml caffeine exposure in 24 hours. It was concluded that coffee extract induce KB cells apoptosis through caspase-3 activation mechanism.
Cell Morphological Change and Caspase-3 Protein Expression on Epithelial Cells under Stimulation of Oral Bacterium Streptococcus sanguinis Hutomo, Suryani; Susilowati, Heni; Agustina, Dewi
Journal of Dentistry Indonesia Vol. 22, No. 1
Publisher : UI Scholars Hub

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Abstract

Oral commensal bacterium Streptococcus sanguinis may find in periodontal lesions, deep seated infection, and infective endocarditis that are usually dominated by anaerobes. This bacterium caused cell death on some cells but host responses to this species remained unclear. Objective: This study was aimed to detect cell morphological change and role of caspase-3 in cell death mechanism induced by S. sanguinis. Methods: HeLa cells as representative model for oral epithelial cells were exposed to 107 cells/ml bacteria for 48 h. Morphological change was observed microscopically after hematoxyline-eosin staining. Expression of active caspase-3 was examined by immunocytochemical analysis after cell stimulation for 36 and 48 h with wild type supragingival S. sanguinis. Doxorubicin (0.5625 μg/ml) was used as positive control for caspase-3 activation. Results: The results showed cell shrinkage of bacterial-treated cells; and active caspase-3 molecules were detected after 36 and 48 hours cell stimulation. Conclusion: This study would suggest cell shrinkage and caspase-3-dependent apoptotic cell death induced by S. sanguinis.
Caspase-3-dependent Cell Death in B lymphocyte Caused by Pseudomonas aeruginosa Pyocyanin Susilowati, Heni; Hutomo, Suryani; Siagian, JW; Siwi, Dyanasti P.
Journal of Dentistry Indonesia Vol. 22, No. 2
Publisher : UI Scholars Hub

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Abstract

Pseudomonas aeruginosa is a Gram negative bacterium that can cause fatal infection in immunocompromised patient. This is an opportunist pathogen which is associated with some dental infections. Pseudomonas aeruginosa produces pyocyanin that functions as an important virulent factor in bacterial invasion. It can be identified in the lesion tissue and capable to induce cellular damage in endothelial cell, respiratory, neutrophil, and lymphocytes. B lymphocyte plays a significant role in the immune response of periapical infection; however, its cellular and molecular response to pyocyanin is unclear. Objective: To investigate cellular responses of B lymphocyte to the exposure of pyocyanin and the role of caspase-3 in its molecular mechanism. Methods: B lymphocytes (Raji cells) were cultured, and in five replications were exposed to various concentrations of pyocyanin for 24 h. MTT assay was performed to analyze the cytotoxicity effect of pyocyanin. Cell morphological analysis using phase contrast microscope were done in separate experiments. Immunocytochemical analysis was carried out for the identification of active caspase-3 protein expression, to study the mechanism involved in pyocyanin-induced cellular damage, Results: It showed that cell viability was decreased in pcyocyanin-treated groups. Pyocyanin induced cell death on B lymphocyte in a dose-dependent manner. Statistical analysis using ANOVA demonstrated significant difference between groups with p=0.000. Nuclear fragmentation was observed in pyocyanin-induced cell death; furthermore, caspase-3 was expressed clearly in cell cytoplasm after 24 h incubation. Conclusion: Pyocyanin is capable of inducing cell death on B l
The Kemampuan Ekstrak Etanol Daun Binahong (Anredera cordifolia (Ten.) Steenis) Dalam Menghambat Pembentukan Biofilm Streptococcus mutans Hutomo, Suryani; Larope, Ceny Gloria
Bahasa Indonesia Vol 24 No 1 (2025): Damianus Journal of Medicine
Publisher : Atma Jaya Catholic University of Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25170/djm.v24i1.6128

Abstract

Pendahuluan: Streptococcus mutans merupakan bakteri predominan pada karies gigi. Bakteri ini mampu membentuk biofilm. Adanya resistensi antibiotik dan biofilm yang menghambat penetrasinya menyebabkan perlunya dilakukan penelitian untuk mengendalikan terbentuknya biofilm. Binahong (Anredera cordifolia) memiliki aktivitas antibakteri dan mudah ditemukan di lingkungan. Tujuan dari penelitian ini adalah untuk menguji efek antibiofilm ekstrak daun binahong (Anredera cordifolia (Ten.) Steenis) terhadap S. mutans sebagai penyebab utama karies gigi. Metode: Ekstrak dipersiapkan menggunakan metode macerasi. Uji antibakteri S. mutans ATCC 25175 menggunakan metode broth microdilution. Pengujian antibiofilm menggunakan metode yang serupa. Biofilm yang terbentuk diwarnai dengan 0,1% crystal violet dan dibaca pada 595 nm menggunakan microplate reader. Hasil: Hasil uji antibakteri menunjukkan bahwa nilai hambat minimum (MIC) ada pada konsentrasi 3750 µg/ml, sedangkan konsentrasi ekstrak sebagai antibiofilm didapatkan mulai dari konsentrasi 187,5 µg/ml sampai dengan 3000 µg/ml. Analisis statistik menggunakan One-way ANOVA menunjukkan perbedaan yang bermakna dari masing-masing kelompok perlakuan (p=0,000). Simpulan: Ekstrak etanol daun binahong dapat menghambat pertumbuhan S. mutans dengan konsentrasi hambat minimum 3750 µg/ml serta menghambat pembentukan biofilm S. mutans pada konsentrasi efektif 750 µg/ml.
Potential immunomodulatory activity of Phyllanthus niruri aqueous extract on macrophage infected with Streptococcus sanguinis Hutomo, Suryani; Putri, Denise Utami; Suryanto, Yanti Ivana; Susilowati, Heni
Dental Journal (Majalah Kedokteran Gigi) Vol. 51 No. 3 (2018): September 2018
Publisher : Faculty of Dental Medicine, Universitas Airlangga https://fkg.unair.ac.id/en

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (407.935 KB) | DOI: 10.20473/j.djmkg.v51.i3.p124-128

Abstract

Background: Streptococcus sanguinis is an oral commensal bacterium commonly found in periodontal lesions and deep abscesses that are usually dominated by anaerobic bacteria. As an important causative agent of systemic diseases, and with the increasingly numerous cases of antimicrobial resistance, some means of modulating the immune response to bacterial infection is thus necessary. Phyllanthus niruri Linn is widely used as a medicinal herb to both prevent and treat disease and demonstrates immunomodulatory properties. Purpose: This study aimed to observe the potential for aqueous extract of Phylanthus niruri to induce macrophage proliferation and NO production following S. sanguinis infection. Methods: Macrophages were isolated from the peripheral blood of healthy subjects, stimulated with P. niruri aqueous extract in graded doses and infected with S. sanguinis ATCC 10556 bacterial suspension. Cell proliferation and nitric oxide release was observed at 24 and 48 hours to determine macrophage activities. Results: NO production and cell proliferation started to increase upon 50 and 100µg/ml P niruri respective stimulation. Statistical analysis using One-way Anova demonstrated a significant difference of cell proliferation after stimulation with P. niruri aqueous extract at various doses (p<0.05). Conclusion: P. niruri aqueous extract induced macrophage proliferation and NO secretion upon S sanguinis infection, showing potential antibacterial and immunomodulatory activities. At the same concentrations, NO production and macrophage were higher at 48 hours than at 24 hours.
Antibacterial and anti-adherence effect of Laportea interupta ethanolic extract on Streptococcus mutans biofilm Hutomo, Suryani; Larope, Ceny Gloria; Putri, Denise Utami; Sooai, Christiane Marlene; Kristiyanto, Haryo Dimasto
Dental Journal (Majalah Kedokteran Gigi) Vol. 58 No. 3 (2025): September
Publisher : Faculty of Dental Medicine, Universitas Airlangga https://fkg.unair.ac.id/en

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/j.djmkg.v58.i3.p256-261

Abstract

Background: Due to its capacity to generate extracellular polymeric substances that aid in biofilm creation, Streptococcus mutans is a leading cause of dental caries. Natural remedies, including traditional plant extracts, are being explored for their antimicrobial potential. Laportea interupta (L. interupta), known as jelatang in Indonesia, is alleged to possess a bacteria-inhibiting effect. Purpose: The purpose of this study is to evaluate the potential use of L. interupta leaf ethanolic extract to inhibit S. mutans proliferation and adherence to hydroxyapatite (HA) discs, simulating the tooth surface. Methods: The broth microdilution approach was utilized to evaluate the minimum concentration of the extract that inhibits bacterial growth. For anti-adherence assessment, HA discs pre-coated with saliva were exposed to different extract concentrations and incubated with S. mutans. Bacterial adhesion was visualized using 0.1% crystal violet staining, quantified spectrophotometrically at 595 nm, and further verified using scanning electron microscopy (SEM). Results: The extract showed inhibitory effects on S. mutans growth at 7,500 μg/ml (MIC). Anti-adherence activity was optimal at 1,500 μg/ml, and SEM analysis confirmed a reduced biofilm formation on extract-treated samples. Conclusion: Laportea interupta ethanolic leaf extract demonstrated both antibacterial and anti-adherence effects against S. mutans, suggesting its potential as a natural anti-biofilm agent for oral health applications.
Pelatihan Pengolahan Tanaman Obat Keluarga (TOGA) Menjadi Kosmetika di Wilayah Kapanewon Sanden Krismi, Arum; Sooai, Christiane Marlene; Kurniawati, Novika; Hutomo, Suryani
Jurnal Atma Inovasia Vol. 5 No. 5 (2025)
Publisher : Lembaga Penelitian dan Pengabdian pada Masyarakat

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24002/jai.v5i5.10365

Abstract

Kosmetika adalah sediaan yang dimaksudkan untuk digunakan pada bagian luar tubuh manusia dengan tujuan antara lain untuk membersihkan tubuh. Salah satu produk kosmetika yang diminati adalah kosmetika yang mengandung bahan rempah, yang banyak ditanam oleh masyarakat sebagai Tanaman Obat Keluarga (TOGA). Tujuan akhir kegiatan pengabdian kepada masyarakat ini adalah meningkatkan pengetahuan tentang manfaat TOGA khususnya untuk kesehatan kulit, serta meningkatkan keterampilan pengolahan TOGA menjadi sediaan kosmetika untuk 90 orang kader Asuhan Mandiri TOGA di wilayah Kapanewon Sanden. Kegiatan pengabdian masyarakat dilaksanakan dengan penyampaian materi mengenai keluhan kulit dan keamanan lulur bahan alam, bentuk sediaan kosmetika, bahan alam yang dapat digunakan untuk kesehatan gigi dan mulut, pembuatan lulur dan sabun dari TOGA, kemudian dilanjutkan dengan praktek pelatihan pengolahan TOGA menjadi lulur. Lulur yang diproduksi oleh kader dan masyarakat telah digunakan oleh masyarakat dan keluarganya
Co-Authors Agustinus Rudolf Phym, Agustinus Arum Krismi, Arum Christiane Marlene Sooai, Christiane Marlene Denise Utami Putri, Denise Utami Devi Chretella Maheswara, Devi Chretella Dewi Agustina Heni Susilowati Kristiyanto, Haryo Dimasto Kurniawati, Novika Larope, Ceny Gloria Siagian, JW Siwi, Dyanasti P. Suryanto, Yanti Ivana