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All Journal Dental Journal (Majalah Kedokteran Gigi)
Denise Utami Putri, Denise Utami
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Dentistry

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Potential immunomodulatory activity of Phyllanthus niruri aqueous extract on macrophage infected with Streptococcus sanguinis Hutomo, Suryani; Putri, Denise Utami; Suryanto, Yanti Ivana; Susilowati, Heni
Dental Journal Vol. 51 No. 3 (2018): September 2018
Publisher : Faculty of Dental Medicine, Universitas Airlangga https://fkg.unair.ac.id/en

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (407.935 KB) | DOI: 10.20473/j.djmkg.v51.i3.p124-128

Abstract

Background: Streptococcus sanguinis is an oral commensal bacterium commonly found in periodontal lesions and deep abscesses that are usually dominated by anaerobic bacteria. As an important causative agent of systemic diseases, and with the increasingly numerous cases of antimicrobial resistance, some means of modulating the immune response to bacterial infection is thus necessary. Phyllanthus niruri Linn is widely used as a medicinal herb to both prevent and treat disease and demonstrates immunomodulatory properties. Purpose: This study aimed to observe the potential for aqueous extract of Phylanthus niruri to induce macrophage proliferation and NO production following S. sanguinis infection. Methods: Macrophages were isolated from the peripheral blood of healthy subjects, stimulated with P. niruri aqueous extract in graded doses and infected with S. sanguinis ATCC 10556 bacterial suspension. Cell proliferation and nitric oxide release was observed at 24 and 48 hours to determine macrophage activities. Results: NO production and cell proliferation started to increase upon 50 and 100µg/ml P niruri respective stimulation. Statistical analysis using One-way Anova demonstrated a significant difference of cell proliferation after stimulation with P. niruri aqueous extract at various doses (p<0.05). Conclusion: P. niruri aqueous extract induced macrophage proliferation and NO secretion upon S sanguinis infection, showing potential antibacterial and immunomodulatory activities. At the same concentrations, NO production and macrophage were higher at 48 hours than at 24 hours.
Antibacterial and anti-adherence effect of Laportea interupta ethanolic extract on Streptococcus mutans biofilm Hutomo, Suryani; Larope, Ceny Gloria; Putri, Denise Utami; Sooai, Christiane Marlene; Kristiyanto, Haryo Dimasto
Dental Journal (Majalah Kedokteran Gigi) Vol. 58 No. 3 (2025): September
Publisher : Faculty of Dental Medicine, Universitas Airlangga https://fkg.unair.ac.id/en

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/j.djmkg.v58.i3.p256-261

Abstract

Background: Due to its capacity to generate extracellular polymeric substances that aid in biofilm creation, Streptococcus mutans is a leading cause of dental caries. Natural remedies, including traditional plant extracts, are being explored for their antimicrobial potential. Laportea interupta (L. interupta), known as jelatang in Indonesia, is alleged to possess a bacteria-inhibiting effect. Purpose: The purpose of this study is to evaluate the potential use of L. interupta leaf ethanolic extract to inhibit S. mutans proliferation and adherence to hydroxyapatite (HA) discs, simulating the tooth surface. Methods: The broth microdilution approach was utilized to evaluate the minimum concentration of the extract that inhibits bacterial growth. For anti-adherence assessment, HA discs pre-coated with saliva were exposed to different extract concentrations and incubated with S. mutans. Bacterial adhesion was visualized using 0.1% crystal violet staining, quantified spectrophotometrically at 595 nm, and further verified using scanning electron microscopy (SEM). Results: The extract showed inhibitory effects on S. mutans growth at 7,500 μg/ml (MIC). Anti-adherence activity was optimal at 1,500 μg/ml, and SEM analysis confirmed a reduced biofilm formation on extract-treated samples. Conclusion: Laportea interupta ethanolic leaf extract demonstrated both antibacterial and anti-adherence effects against S. mutans, suggesting its potential as a natural anti-biofilm agent for oral health applications.
Co-Authors Christiane Marlene Sooai, Christiane Marlene Heni Susilowati Kristiyanto, Haryo Dimasto Larope, Ceny Gloria Suryani Hutomo, Suryani Suryanto, Yanti Ivana