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All Journal Indonesian Journal of Halal Research Journal of Advanced Technology and Multidiscipline (JATM)
Zein, Muhammad Ihda Hamlu Liwaissunati
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Religion Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Computer Science & IT Electrical & Electronics Engineering Environmental Science Industrial & Manufacturing Engineering Materials Science & Nanotechnology Medicine & Pharmacology Other

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Methods Validation of Pork Authentication in Processed Meat Products (Sausages) Through Densitometry Analysis Hermanto, Sandra; Rudiana, Tarso; Zein, Muhammad Ihda Hamlu Liwaissunati; Wisudawati, Asih Widi
Indonesian Journal of Halal Research Vol. 4 No. 1 (2022): February
Publisher : UIN Sunan Gunung Djati Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15575/ijhar.v4i1.11892

Abstract

Analysis of the halalness of food products based on animal origin needs to be carried out, especially for critical food products such as processed meat products (sausages). Some processed meat products are found that they are contaminated by pork in the processing. This research was conducted to determine the validity of the analysis method of pork contamination in one of the processed products (beef, pork, and goat sausages) based on the characteristics of the protein profile. Beef, pork, and goat sausages that have been purchased from supermarkets in Jakarta and South Tangerang are used as the sample.The praparation of sample was preceded by extraction of the sausage protein in PBS buffer pH 7.2. Then, the dissolved protein content was tested, and the protein extract was separated by SDS-PAGE (sodium dodecyl sulphate polyacrylamide gel electrophoresis). The protein profile result from SDS-PAGE separation was followed by densitometric analysis (ImageJ) to obtain biomarker protein candidates. The validation of the analytical method includes the precision (repeatability), test for accuracy, linearity, and robustness of the method by varying the extraction pH. The results show that the protein extract has the differences in protein content and composition for each sample. The SDS-PAGE analysis results show that biomarker protein candidates appeared below 50 kDa which were thought to be the protein fraction of actin. The precision and accuracy test results obtained for each sample have met the required standards, namely with a KV value <5% and a percent recovery value> 95%. The results of the linearity test and the toughness of the method also show that the test method is quite effective in testing the halalness of animal food products, especially in sausage products which are contaminated by pork.
Electrochemical Sensor and Biosensor Detection of Ethanol in Beverage Samples: Irkham, Irkham; Zalfadilah, Faizal Nur; Zein, Muhammad Ihda Hamlu Liwaissunati; Khaerani, Wulan; Zakiyyah, Salma Nur; Hartati, Yeni Wahyuni
Journal of Advanced Technology and Multidiscipline Vol. 4 No. 1 (2025): Journal of Advanced Technology and Multidiscipline
Publisher : Faculty of Advanced Technology and Multidiscipline Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jatm.v4i1.71985

Abstract

Ethanol detection is critical in the beverage industry, where it is essential to monitor alcohol concentrations for quality control and compliance with regulatory standards. Traditional analytical methods, such as gas chromatography and distillation, offer accuracy but are often labor-intensive, time-consuming, and require sophisticated equipment. In contrast, electrochemical sensors and biosensors have emerged as promising alternatives due to their rapid response, portability, cost-effectiveness, and potential for real-time monitoring. Electrochemical sensors, particularly those enhanced with metal nanoparticles like platinum, palladium, or gold, have shown significant improvements in sensitivity, selectivity, and response time. These sensors offer the advantage of miniaturization, making them ideal for on-site analysis, although issues such as electrode stability, susceptibility to interference, and long-term reliability remain. On the other hand, biosensors, which leverage biorecognition elements like alcohol dehydrogenase (ADH) or alcohol oxidase, provide high specificity for ethanol, reducing interference from other compounds commonly found in beverage samples. Recent advancements in biosensor technology have focused on improving sensor stability, enzyme immobilization techniques, and reducing production costs. While biosensors offer high selectivity and sensitivity, they may still face challenges related to enzyme denaturation and environmental factors such as temperature and pH fluctuations. Both electrochemical sensors and biosensors are continuously evolving, with recent developments including the use of nanomaterials and novel biorecognition elements to enhance performance. This review will explore recent advances in electrochemical sensors and biosensors for ethanol detection in beverage samples, highlighting their potential, challenges, and future directions in this field.
Co-Authors Asih Widi Wisudawati Irkham, Irkham Khaerani, Wulan Sandra Hermanto Tarso Rudiana, Tarso Yeni Wahyuni Hartati Zakiyyah, Salma Nur Zalfadilah, Faizal Nur