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All Journal Jurnal Ilmu Ternak Veteriner JITRO (Jurnal Ilmiah dan Teknologi Peternakan Tropis) JITPI : Jurnal Ilmu Dan Teknologi Peternakan Indonesia (Indonesian Journal of Animal Science and Technology)
Chairussyuhur Arman
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Agriculture, Biological Sciences & Forestry Veterinary

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Identification of Bmpr-1b and Bmp15 gene mutations in fat tail sheep ., Maskur; Arman, Chairussyuhur
Indonesian Journal of Animal and Veterinary Sciences Vol 15, No 1 (2010)
Publisher : Indonesian Animal Sciences Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (304.132 KB) | DOI: 10.14334/jitv.v15i1.673

Abstract

Fat tail sheep (FTS) is regarded as highly prolific local sheep and have been well adapted under tropical climate of Lombok island. BMPR-1B and BMP15 genes that controll reproductive traits such as ovulation rate and litter size in different type of sheep will be studied as candidate genes for prolific traits in FTS. These genes  have been reported by various investigators have different prolificacy mechanism between several breeds of sheep, and it is very likely will occur in FTS. This study was designed to understand different prolificacy mechanism that occurred among breed of sheep. One hundred and forty FTS potential for twin and triplet scattered in Lombok Island, West Nusa Tenggara Province were screened to identify mutation of BMPR-1B and BMP15 genes using Forced PCR-RFLP method. Furthermore, the frequency of allel and genotype caused by mutation was measured in each gene. Results of the study indicated that mutation of FecXG at BMP15 gene resulted in two alleles, namely “wild-type”(+) Allel  was 111 bp and 30 bp, and mutan allel (G) was 141 bp with frequency of 0.675 and 0.325. The combination of allel at BMP15 gen resulted in two genotipes, namely: ++ (111 bp/111 bp) and G+ (141 bp/111 bp) with frequency of 0.35 dan 0.65 in FTS. Mutation of FecB at BMPR-1B gen resulted in two allel, namely “wild-type”(+) Allel was 140 bp, and mutan allel (B) was 110 and 30 bp with frequency of 0.718 and 0.282. The combination of allel at BMPR-1B gen resulted in three genotipes, namely: BB (110 bp/110 bp), B+ (110 bp/140 bp), and ++ (140 bp/140 bp), its frequency was 0.11, 0.35 and 0.54 in FTS, respectively. Key words: Mutation, BMPR-1B, BMP15, Fat Tailed, Sheep
Efektifitas Kinerja Pentoksifilin Terhadap Kualitas dan Integritas Membran Plasma Utuh pada Sperma Sapi Bali Hasil Pemisahan dengan Menggunakan Albumin Rodiah Rodiah; Enny Yuliani; Adji Santoso Dradjat; Chairussyuhur Arman
Jurnal Ilmu Dan Teknologi Peternakan Indonesia (JITPI) Indonesian Journal of Animal Science and Technology) Vol 1 No 1 (2015): Jurnal Ilmu dan Teknologi Peternakan Indonesia (JITPI)
Publisher : Faculty of Animal Husbandry, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (200.932 KB) | DOI: 10.29303/jitpi.v1i1.12

Abstract

The purpose of this research was to determine the effect of Pentoxyfilline in maintaining the quality (motility, viability, morphology and plasma membrane intact) of Bali bull spermatozoa separated with albumine. A Completely Random Block Design was used in this study with two main factors (upper layer and bottom layer), diluents with four doses of pentoxyfilline (0, 15, 20 and 25 μM) with six replications. Evaluation of spermatozoa was performed by characterizing motility, viability, morphology, plasma membrane intact. Result of the study showed that the effect of four doses pentoxyfilline on the quality of Bali bull spermatozoa was non significant (p>0,05). However, the addition of 25μM pentoxyfilline was found to give the best effect compared to 15 μM, 20 μM and control. Result of the study also indicated that spermatozoa separated in the upper layer was signifiqantly higher (p<0,01) than that of separated in the bottom layer with the average motility, viability, morfology and plasma membrane intact was 66,67,80,50, 6,71 and 65,61% respectively.
Identifikasi Komponen, Faktor dan Resiko Relatif yang Berpengaruh pada Hasil Inseminasi Buatan pada Sapi Bali Adji Santoso Dradjat; Rodiah Rodiah; Enny Yuliani; Chairussyuhur Arman
Jurnal Ilmu Dan Teknologi Peternakan Indonesia (JITPI) Indonesian Journal of Animal Science and Technology) Vol 2 No 1 (2016): Jurnal Ilmu dan Teknologi Peternakan Indonesia (JITPI)
Publisher : Faculty of Animal Husbandry, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (166.266 KB) | DOI: 10.29303/jitpi.v2i1.26

Abstract

The aims of the research were to identify components, factors and relative risk (Odd ratio) which may influencethe artificial insemination results. The study were performed in two villages those were Kuranji and Tanah Beak,by identification of components and factors which may contribute to successful artificial insemination results,then the flow chart of successful and obstacle were designed. Based on the flow chart survey was performed to thefarmers and inseminators. Parameters evaluated were factors may influence of the result of insemination such as;management, oestrus detection by farmers and insemination by inseminators. The data was tabulated and analisedusing epidemiology analysis of odd ratios (OR). The results showed that insemination results of Kuranji villagedo not have the risk to be not pregnant as the odd ratio was less than 1 (OR <1,0), with the range of 0,10 to 0,20.While artificial insemination in Tanah Beak village showed risk of cows to be infertile or non pregnant relativelyhigher with OR higher than 1 (OR> 1.0), with the range of 1,5 to 2,0. The results showed that the risk to beunsuccessful to produce pregnancy were caused by oestrus detection, feeding management, skill inseminator andexperience of farmers. Finally it can be concluded that oestrus detection by farmers, feeding management,insemination by inseminator and experience of farmers may contribut to the high risk fail to produce pregnancyusing artificial insemination in Tanah beak village.
Identification of Bmpr-1b and Bmp15 gene mutations in fat tail sheep Maskur .; Chairussyuhur Arman
Jurnal Ilmu Ternak dan Veteriner Vol 15, No 1 (2010): MARCH 2010
Publisher : Indonesian Center for Animal Research and Development (ICARD)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (304.132 KB) | DOI: 10.14334/jitv.v15i1.673

Abstract

Fat tail sheep (FTS) is regarded as highly prolific local sheep and have been well adapted under tropical climate of Lombok island. BMPR-1B and BMP15 genes that controll reproductive traits such as ovulation rate and litter size in different type of sheep will be studied as candidate genes for prolific traits in FTS. These genes  have been reported by various investigators have different prolificacy mechanism between several breeds of sheep, and it is very likely will occur in FTS. This study was designed to understand different prolificacy mechanism that occurred among breed of sheep. One hundred and forty FTS potential for twin and triplet scattered in Lombok Island, West Nusa Tenggara Province were screened to identify mutation of BMPR-1B and BMP15 genes using Forced PCR-RFLP method. Furthermore, the frequency of allel and genotype caused by mutation was measured in each gene. Results of the study indicated that mutation of FecXG at BMP15 gene resulted in two alleles, namely “wild-type”(+) Allel  was 111 bp and 30 bp, and mutan allel (G) was 141 bp with frequency of 0.675 and 0.325. The combination of allel at BMP15 gen resulted in two genotipes, namely: ++ (111 bp/111 bp) and G+ (141 bp/111 bp) with frequency of 0.35 dan 0.65 in FTS. Mutation of FecB at BMPR-1B gen resulted in two allel, namely “wild-type”(+) Allel was 140 bp, and mutan allel (B) was 110 and 30 bp with frequency of 0.718 and 0.282. The combination of allel at BMPR-1B gen resulted in three genotipes, namely: BB (110 bp/110 bp), B+ (110 bp/140 bp), and ++ (140 bp/140 bp), its frequency was 0.11, 0.35 and 0.54 in FTS, respectively. Key words: Mutation, BMPR-1B, BMP15, Fat Tailed, Sheep
Substitusi Kuning Telur dengan Lesitin Kedelai sebagai Pengencer Semen dalam Mempertahankan Kualitas Spermatozoa Kerbau Penyimpanan 5°C Nifsu Sabaan; Chairussyuhur Arman; Enny Yuliani; Maskur Maskur
Jurnal Ilmu dan Teknologi Peternakan Tropis Vol 6, No 3 (2019): JITRO, September
Publisher : Universitas Halu Oleo

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (566.966 KB) | DOI: 10.33772/jitro.v6i3.8173

Abstract

ABSTRAKPengencer konvensional berbasis kuning telur yang bersumber dari hewan membawa risiko kontaminasi mikroba dan menghalangi jarak pandang pemeriksaan spermatozoa di bawah mikroskop. Penelitian ini dilaksanakan` untuk menilai apakah substitusi kuning telur dengan lesitin kedelai di dalam pengencer berbasis-tris dapat mempertahankan kualitas spermatozoa kerbau lumpur pada penyimpanan 5°C selama 120 jam. Ejakulat dari 3 ekor kerbau umur 3-4 tahun dengan bobot badan 450-500 kg ditampung dengan menggunakan vagina buatan.Semen diencerkan dengan pengencer konvensional yang mengandung 20% kuning telur (kontrol) dan pengencer lesitin dengan konsentrasi1%, 2%, 3% dan 4%(perlakuan) dan disimpan pada 5°C selama 120 jam. Parameter spermatozoa dinilai pada interval 0, 72, dan 120 jam setelah semen yang diencerkan disimpan 5°C.Persentase motilitas dan spermatozoa normal tidak berbeda nyata (P<0,05) antara pengencer 1% lesitin dan kontrol. Namun pengencer 1% lesitin mampu mempertahankan kedua parameter tersebut setelah penyimpanan 5°C selama 120 jam. Persentase viabilitas, keutuhan membran dan tudung akrosom spermatozoa pada pengencer 1% lesitin dibandingkan pengencer kontrol lebih tinggi (P<0,05) dari pengencer perlakuan 2, 3, dan 4% lesitin. Disimpulkan bahwa pengencer 1% lesitin dapat menyubstitusi pengencer konvensional berbasis kuning telur untuk penyimpanan semen kerbau pada 5ºC selama 120 jam.Kata kunci: kuning telur, lesitin, pengencer, semen cair, semen kerbauABSTRACTConventional egg yolk-based diluent animal origin carries the risk of microbial contamination and interference with microscopic examination. This study was conducted to assess whether substitution of egg yolk with soy lecithin in tris-based diluents can maintain the quality of buffalo spermatozoa stored at 5°C for 120 h. Ejaculate from 3 buffaloes aged 3-4 years and body weight of 450-500 kg was collected using an artificial vagina. Semen was diluted with diluent containing 20% egg yolk (control) and diluent with concentrations of lecithin 1%, 2%, 3% and 4% (treatment). Spermatozoa parameters were assessed at intervals of 0, 72 and 120 h after the diluted semen was stored at 5°C for 120 h. The percentages of motility and normal spermatozoa did not differ significantly (P<0.05) between 1% lecithin and control diluents. However, 1% of lecithin was able to maintain both parameters after stored at 5°C for 120 h. The percentages of viability, membrane integrity and functional acrosome of spermatozoa in 1% lecithin were higher (P<0.05) than control and the other three treatment diluents. In conclusion, 1% of lecithin diluent could substitute conventional egg yolk-based diluent for the storage of buffalo semen at 5ºC for 120 h.Keywords: buffalo semen, egg yolk, lecithin, diluents, liquid semen
Co-Authors Adji Santoso Dradjat Adji Santoso Dradjat Enny Yuliani Maskur . Maskur Maskur Nifsu Sabaan Rodiah Rodiah Rodiah Rodiah