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All Journal Indonesian Journal of Clinical Pathology and Medical Laboratory (IJCPML) Indian Journal of Forensic Medicine & Toxicology
Joewono Soeroso
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Biochemistry, Genetics & Molecular Biology Health Professions Medicine & Pharmacology Neuroscience Public Health

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Association of Plasma Interferon-a(IFN-a) with C-Reactive Protein (CRP) Level and Disease Activity in Systemic Lupus Erythematosus (SLE) Patients Awalia; Harianto Notopuro; Joewono Soeroso
Indian Journal of Forensic Medicine & Toxicology Vol. 16 No. 2 (2022): Indian Journal of Forensic Medicine & Toxicology
Publisher : Institute of Medico-legal Publications Pvt Ltd

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37506/ijfmt.v16i2.17942

Abstract

Background: CRP is normal or only slightly increased in active SLE. It is presumed that IFN-α mayinhibit the transcription process during CRP synthesis. There is also increasing in IFN-αgene expressionin active SLE. This study examined the correlation of plasma IFN-α with CRP and SLE activity.Methods:Forty SLE patients were included. SLAM and SLEDAI were used to measure SLE diseaseactivity. Laboratory tests were examined at dr.Soetomo Hospital Surabaya. CRP was measured usingimmunoturbidimetry. C3 and C4 were measured by radial immunodiffusion technique. IFN-αwasmeasured using ELISA.Results and conclusion:Twenty-six patients from the outpatient clinic and 14 from wards wereincluded from August 2019 to February 2020. The median age was 31.5 years old. The median SLAMscore was 8.5. Mean CRP was 5.19±2.69 mg/L. Median plasma IFN-α was 46.02 (16.43-177.96).Spearman correlation test revealed a moderate negative correlation between plasma IFN-α and CRPlevel (p=0.003; r=-0.455). A moderate positive correlation was showed between plasma IFN-α leveland SLAM score (p=0.001; r=0.568). No correlation found between CRP and SLAM. There was astrong correlation between complement levels with SLEDAI. Linear regression revealed a significantassociation of IFN-α and C3 (not C4) level with SLEDAI.
Green Tea Suppresses Serum TNF-? and TGF-?1 Levels In Mice Model of Systemic Lupus Erythematosus Herin Mawarti; Jusak Nugraha; Djoko Agus Purwanto; Joewono Soeroso
Indian Journal of Forensic Medicine & Toxicology Vol. 15 No. 2 (2021): Indian Journal of Forensic Medicine & Toxicology
Publisher : Institute of Medico-legal Publications Pvt Ltd

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37506/ijfmt.v15i2.14746

Abstract

Objective: This study aims to investigate the effects of green tea administration on TNF-a, Hsp70, andTGF-b1 levels in the systemic lupus erythematosus (SLE). Material and methods: A total of 32 micewill be divided into four groups (each 8 mice), namely the control group, the SLE group, the SLE groupwho were given green tea extract at a dose of 500 mg/kg body weight, and the SLE group who were givengreen tea extract at a dose of 1000 mg/kg body weight. Analysis of TNF-a, Hsp70, and TGF-b1 levels wascarried out using the enzyme-linked immunosorbent assay technique. Results: TNF-a and TGF-b1 levelswere significantly increased in the SLE group compared to the control group (p < 0.05). This increase canbe significantly reduced through the provision of green tea, even reaching levels comparable to the controlgroup (p > 0.05). Conclusions: It was concluded that green tea containing EGCG can suppress TNF-a andTGF-b1 in the SLE model. Thus, green tea can be an alternative in immunology modulation in SLE.
Environmental Factors and Protective Effects of Epigallocatechin-3-Gallate to Systemic Lupus Eritematosus: A Review Study Herin Mawarti; Jusak Nugraha; Djoko Agus Purwanto; Joewono Soeroso
Indian Journal of Forensic Medicine & Toxicology Vol. 15 No. 2 (2021): Indian Journal of Forensic Medicine & Toxicology
Publisher : Institute of Medico-legal Publications Pvt Ltd

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37506/ijfmt.v15i2.14750

Abstract

SLE (Systemic Lupus Erythematosus) is one of autoimmune disease in which the immune system attacksits own tissues, causing widespread inflammation and tissue damage in the affected organs. Etiology andpathogenesis are not clearly mention until now by the scientist, but environmental factor is contribute todevelopment of Lupus and Flare disease. The objective of this study to explain the relationship betweenenvironmental factors and pathogenesis development of SLE, and how the Epigallocatechin-3-Gallate(EGCG) can protect or prevent of organs damage and environmental effects.Method: The research design of this study was used literature review. The data sources collected fromElectronic database in Pubmed, Sage, Google Scholar and website of science including reports, journals andmostly published in the last 10 years.Results: The pathomechanism of SLE was influenced by environmental factors and this was caused anincreasing of oxidative stress, chronic inflammation, dysregulation of immune system and decreased theclean of immune complex and apoptosis of cells. The production of autoantibodies and immune complex arerelated with target tissue with is it was caused chronic inflammation and the end it caused irreversible damagein glomerulus of the kidneys, arteries, lungs and other tissues. EGCG plays a protecting role in environmentalfactors as a trigger because it works as an antioxidant, anti-inflammatory and immunomudulator. So EGCGcan as a potential agent to protect SLE.Conclusion: Environmental factors plays an important role as a triger of SLE and flare. Moreover, EGCG asa potential agent to protect the presence of oxidative stress, inflammation and immune dysregulation.
ASOSIASI HLA-DRB1* DAN HLA-DQB1* DENGAN IGM-RF SERUM PADA ARTRITIS REUMATOID Joewono Soeroso; Ferdinandus Maria Judajana; Handono Kalim
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol 18, No 3 (2012)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v18i3.373

Abstract

Rheumatoid arthritis (RA) is a chronic progressive polygenic autoimmune disease with a high socio-economic burden. Genetic polymorphisms expressed as a shared epitope (SE) at hypervariable regions of HLA-DRB1*01/*04/*10/*14 and linkage disequilibriums of HLA-DRB1*-HLA-DQB1* with some microorganisms, increase the risk of RA. Shared epitopes are also related with the increase of serum IgM-RF as well. A cross-sectional study on 48 RA patients diagnosed by ACR Criteria 1987 from 6 hospitals in Java and Bali was done to determine the associations of HLA-DRB1* and HLA-DQB1* alleles with serum IgM-RF levels. The presence of HLA-DRB1* and HLA-DQB1* alleles were assessed through PCR-SSO. Serum IgM-RF levels (IU/mL) were assessed through ELISA. Man-Whitney U tests were used to measure the associations. Value of p<0.05 is considered to be statistically significant. The results showed that mean ranks IgM-RF levels of subjects who are bearing HLA-DRB1*04 compared to subjects who are not bearing HLA-DRB1*04 were 34.10 IU/ml (n=10) vs 21.97 IU/ml (n=38), p=0.014. While, the mean ranks of IgM-RF levels of subjects who are bearing haplotype combination of HLA-DRB1*04-HLA-DQB1*03 vs subjects who are not bearing haplotype combination of HLA-DRB1*04- HLA-DQB1*03 were 32.89 IU/mL (n=9) vs 22.56 IU/mL (n=39) (p=0.046). It is concluded that there are association of HLA-DRB1*04, and haplotype combination of HLA-DRB1*04-HLA-DQB1*03 with increased levels of IgM-RF among Indonesians with RA.
Co-Authors Awalia Djoko Agus Purwanto Ferdinandus Maria Judajana Handono Kalim Harianto Notopuro Herin Mawarti Jusak Nugraha