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All Journal Folia Medica Indonesiana Dental Journal (Majalah Kedokteran Gigi) The Indonesian Biomedical Journal Jurnal Manajemen Kesehatan Yayasan RS.Dr. Soetomo Jurnal Media Analis Kesehatan YARSI Medical Journal INDONESIAN JOURNAL OF MEDICAL LABORATORY SCIENCE AND TECHNOLOGY Biomolecular and Health Science Journal JUXTA: Jurnal Ilmiah Mahasiswa Kedokteran Universitas Airlangga Indian Journal of Forensic Medicine & Toxicology Berkala Penelitian Hayati

Harianto Notopuro

Department Of Biochemistry, School Of Medicine And Tropical Disease Centre Airlangga University, Surabaya.

Author-ID : 331862

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Dentistry Health Professions Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology Neuroscience Public Health Social Sciences Other

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Karsinoma Nasopharynx dan infeksi EBV di Indonesia; analisis aspek klinis, Patologi dan Biomolekular Notopuro, Harianto; A Kentjono, Widodo; Handajani, Retno; B Notopuro, Paulus
Jurnal Kedokteran YARSI Vol 16, No 2 (2008): MEI - AGUSTUS 2008
Publisher : Lembaga Penelitian Universitas YARSI

Nasopharyngeal carcinoma (NPC) is a unique epithelial malignancy that occurs at a high frequency in certain regions of Southeast Asia. Previous study revealed the association between Epstein Barr Virus (EBV) and to a lesser extent, Human Papilloma Virus (HPV) with NPC. The role of the EBV in the pathogenesis of NPC was further supported by the recovery response of the tumor cells after being treated with EBV-specific T lymphocytes. The present study aims to determine the frequency distribution of EBV infection among the pathologically confirmed NPC patients and healthy control in Surabaya, Indonesia. The presence of Human Papilloma. Virus infection (HPV) was also analysed. The results indicated that EBV DNA existed in the 88% of the nasopharyngeal tissue biopsies of 25 NPC patients and none in the 10 healthy controls, and the difference was statistically significant.Analysis of the mononuclear cells of peripheral blood revealed that 60% of the 25 NPC patients carried EBV DNA whereas in control group 20% was found to be positive and the difference was statistically significant. Although a high positive rate EBV-DNA was detected in NPC patients, additional environmental and genetic factors must still be considered. Nevertheless, no HPV-DNA was detected from mononuclear cells of peripheral blood and nasopharyngeal tissue of the two groups. By this study there was no coexistence between the infection of EBV and HPV which promote carcinogenesis in NPC. Analysis using LMP1-DNA in tumor cell biopsies indicated that 72% of the NPC patients yielded PCR products and none of the healthy control, the difference was statistically significant. In conclusion the findings confirm the tight association between the EBV and NPC in Indonesia and that the specific presence of LMP1-DNAs in the tumor cells strongly indicates the important role of EBV in the pathogenesis of NPC. Coexistence of EBV and HPV infections was not found in NPC cases in Indonesia.

Association of Plasma Interferon-a(IFN-a) with C-Reactive Protein (CRP) Level and Disease Activity in Systemic Lupus Erythematosus (SLE) Patients Awalia; Harianto Notopuro; Joewono Soeroso
Indian Journal of Forensic Medicine & Toxicology Vol. 16 No. 2 (2022): Indian Journal of Forensic Medicine & Toxicology
Publisher : Institute of Medico-legal Publications Pvt Ltd

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37506/ijfmt.v16i2.17942

Background: CRP is normal or only slightly increased in active SLE. It is presumed that IFN-α mayinhibit the transcription process during CRP synthesis. There is also increasing in IFN-αgene expressionin active SLE. This study examined the correlation of plasma IFN-α with CRP and SLE activity.Methods:Forty SLE patients were included. SLAM and SLEDAI were used to measure SLE diseaseactivity. Laboratory tests were examined at dr.Soetomo Hospital Surabaya. CRP was measured usingimmunoturbidimetry. C3 and C4 were measured by radial immunodiffusion technique. IFN-αwasmeasured using ELISA.Results and conclusion:Twenty-six patients from the outpatient clinic and 14 from wards wereincluded from August 2019 to February 2020. The median age was 31.5 years old. The median SLAMscore was 8.5. Mean CRP was 5.19±2.69 mg/L. Median plasma IFN-α was 46.02 (16.43-177.96).Spearman correlation test revealed a moderate negative correlation between plasma IFN-α and CRPlevel (p=0.003; r=-0.455). A moderate positive correlation was showed between plasma IFN-α leveland SLAM score (p=0.001; r=0.568). No correlation found between CRP and SLAM. There was astrong correlation between complement levels with SLEDAI. Linear regression revealed a significantassociation of IFN-α and C3 (not C4) level with SLEDAI.

Effects of Ethanol Extract of Rosa damascene on HbA1c Level and NF-κB Expression in Diabetic Rats Choirotussanijjah Choirotussanijjah; Harianto Notopuro; Ema Qurnianingsih
JURNAL INDONESIA DARI ILMU LABORATORIUM MEDIS DAN TEKNOLOGI Vol 3 No 2 (2021): Molecule analysis to advance laboratory diagnosis
Publisher : Universitas Nahdlatul Ulama Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33086/ijmlst.v3i2.2151

Diabetes mellitus (DM) is a chronic hyperglycemic that can cause complications in several organs. It could lead glycosylated hemoglobin or HbA1c formation which has ability undergo further changes into Advanced Glycation End Products (AGEs) and stimulate activation of transcription factors such as nuclear factor kappa B (NF-κB). Some studies showed that anthocyanin has antioxidant and anti-inflammatory activity and it has been found Rosa damascene contain high level of anthocyanin. Total anthocyanin content was 0.459 ± 0.003 mg/L. The aim of this study was to investigate inhibitory effect of R. damascena extract on HbA1c levels and NF-κB activation in diabetic rats. Male wistar rats (n=24) were divided into 6 groups as normal control (KN), streptozotocin-induced diabetic control (KP), diabetic treated with R. damascene (P1, P2, P3; 250, 500 and 1000 mg/kg/d respectively) and diabetic treated with metformin (KM; 500 mg/kg/d). This was carried out for 28 days. The inhibition mode of R. damascene extract was examined by measuring HbA1c levels and expression of NFκB by immunohistochemistry. The results showed p values > 0.05 for HbA1c and p < 0.05 for NFκB. From immunohistochemical staining, it seen the expression of NFκB was low in treated group (P1, P2, P3 and KM) compared with KP. Thus, oral administration of R. damascene extract for 28 days could not reduce HbA1c levels, but can supress NFκB expression.

Molecular detection of interleukin-1A +4845 G→T gene in aggresive periodontitis patients Chiquita Prahasanti; Harianto Notopuro
Dental Journal (Majalah Kedokteran Gigi) Vol. 45 No. 4 (2012): December 2012
Publisher : Faculty of Dental Medicine, Universitas Airlangga https://fkg.unair.ac.id/en

Background: Abundant researches had been conducted based on the clinical and histopathological pathogenesis of aggresive periodontitis. Nevertheless, there were still few researches which based on molecular biology, and especially related to gene polymorphism. This study was done based on IL-1A +4845G→T gene polymorphism in aggressive periodontitis patients. Purpose: The purpose of this tudy was to characterized the generic variation of IL-1A +4845G→T as a risk factor aggressive periodontitis and chronic periodontitis. Methods: DNA from patients with aggressive periodontitis and chronic periodontitis was taken determination of IL-1A +4845 G→T polimorphism was conducted with PCR-RFLP technique. Results: Homozygous allele TT polymorphism was not found in all samples, only allele GG (wild type) and allele GT (heterozygous mutant) were not affect aggressive periodontitis and chronic periodontitis. Conclusion: The study showed there was no significant association between IL-1A +4845G→T gene polymorphism and aggressive periodontitis and chronic periodontitis. Latar belakang: Penelitian tentang patogenesa periodontitis agresif berdasar klinis dan histopatologi telah banyak dilakukan, akan tetapi penelitian berdasar biologimolekuler terutama polimorfisme gen masih sangat jarang dilakukan. Penelitian ini dilakukan berdasarkan pada polimorfisme gen IL-1A +4845G→T pada penderita periodontitis agresif. Tujuan: Tujuan dari penelitian ini adalah untuk mengetahui variasi genetik dari IL-1A +4845G→T yang merupakan faktor risiko periodontitis agresif dan periodontitis kronis. Metode: DNA dari penderita periodontitis agresif dan periodontitis kronis diisolasi, selanjutnya dilakukan determinasi dari polimorfisme gen IL-1A +4845G→T dengan menggunakan teknik PCR-RFLP. Hasil: Pada seluruh sampel penelitian ini tidak dijumpai polimorfisme allel TT (homosigot mutan), yang didapat adalah jenis allel GG (wild type) dan allel GT (heterosigot mutan) yang tidak berpengaruh terhadap periodontitis agresif dan periodontitis kronis. Kesimpulan: Polimorfisme gen IL-1A +4845G→T tidak mempunyai hubungan terhadap kejadian periodontitis agresif dan periodontitis kronis.

MTHFR C677T and TS 5’-UTR 3R/3R Gene Polymorphism in Methotrexate-Resistant Childhood Acute Lymphoblastic Leukemia I Dewa Gede Ugrasena; Harianto Notopuro; Subijanto Marto Sudarmo; Ketut Sudiana; Djajadiman Gatot; Ponpon Idjradinata
The Indonesian Biomedical Journal Vol 12, No 2 (2020)
Publisher : The Prodia Education and Research Institute (PERI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18585/inabj.v12i2.1109

BACKGROUND: Childhood acute lymphoblastic leukemia (ALL) is the most common pediatric malignancy in Indonesia and often treated by methotrexate (MTX). Though it can be cured in 30-60% of patients, MTX resistance remains the major cause of treatment failure in childhood ALL. Previous sudies showed that its anti-leukemic property was moderated by MTX ability to inhibitmethylene tetra hydrofolate reductase (MTHFR) and thymidylate synthase (TS) in folate metabolism. This study investigates the correlation between MTHFR and TS polymorphism and MTX resistance in ALL children.METHODS: A total of 155 subjects obtained from all subjects prior to chemotherapy. DNA from blood samples were extracted and underwent polymerase chain reactionrestriction fragment length polymorphism (PCR-RFLP) to evaluate MTHFR C677T and TS 5’-UTR 3R/3R polymorphism.RESULTS: There was significant correlation between MTHFR C677T and TS 5’-UTR 3R/3R gene polymorphism with MTX resistance. Subjectswith MTHFR C677T and TS 5’-UTR 3R/3R gene polymorphism were 4 times (p=0.007) and 6.4 times (p=0.001) more likely to be MTX resistant than those without gene polymorphisms, respectively.CONCLUSION: MTHFR C677T andTS 5’-UTR 3R/3R represent dominant gene polymorphism related to MTX resistance in childhood ALL.KEYWORDS: gene polymorphism, folate metabolism, acute lymphoblastic leukemia

The Improvement of Community Knowledge, Attitudes and Practices After Covid-19 Socialization Siti Khaerunnisa; Irmi Syafa'ah; Citrawati Dyah Kencono Wungu; Gwenny Ichsan Prabowo; Retno Handajani; Indri Safitri; Harianto Notopuro; Ema Qurnianingsih; Lina Lukitasari; Ira Humairah; Arief Bakhtiar; Suwandito Suwandito; Susi Wahyuning Asih; Zuhrotul Eka Yulis Anggraeni; Ginanjar Sasmito Adi; Ely Rahmatika Nugrahani; Ayesie Natasha Zulka; Soetjipto Soetjipto
Folia Medica Indonesiana Vol. 57 No. 2 (2021): June
Publisher : Universitas Airlangga

This study determined community knowledge, attitudes, and practices after COVID-19 socialization in Rambipuji and Suci Village, Jember District, East Java, Indonesia. This study used the analytic observational design study. As many as 40 people were given socialization about COVID-19 by gathering and online. The questionnaires were completed in two parts included before-socialization and after-socialization. The questionnaire data were analyzed descriptively by calculating frequency, percentage, and inferential statistics by t-test, Spearman correlation, and chi-square test. The data analysis used IBM SPSS version 23 software. The mean and standard deviation of percentage of knowledge pre-test, knowledge post-test, high attitude, moderate attitude, low attitude, very high practice, high practice, sufficient practice, and low practice were 58.33 ± 30.97, 77.70 ± 22.52, 62.5±21.7, 31±22.2, 6.5±17.1, 65±13.8, 32.3±13.8, 1.25±2.4, 1.25±1.8, respectively. Knowledge pre-test and post-test correlated 0.819 (p=0.001) and a t-test with p=0.003. Attitudes and practices correlated with p=0.001. Socialization of COVID-19 was useful to improve the community knowledge, attitudes, and practices in Rambipuji and Suci Village, Jember District, East Java, Indonesia that could prevent the transmission and inhibit the spread of the COVID-19 pandemic in Indonesia. Furthermore, continuous encouragement of COVID-19 socialization in wide areas was recommended.

The Effect of Trivalent Influenza Vaccines on Immunoglobulin G and Interleukin 4 in Ferret (Mustela putorius furo) Lestari Dewi1,2,, Erman Tritama3 , Reviany Vibrianita Nidom4,5, Kuncoro Puguh Santoso6 , Sri Agus Su
Indian Journal of Forensic Medicine & Toxicology Vol. 14 No. 4 (2020): Indian Journal of Forensic Medicine & Toxicology
Publisher : Institute of Medico-legal Publications Pvt Ltd

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37506/ijfmt.v14i4.12137

The seasonal influenza virus has infected 5-15% of the human population every year, resulting in 250,000-500,000 deaths worldwide. The seasonal influenza epidemic that occurs every year is caused by thecontinuous evolution of seasonal influenza viruses, which allows them to avoid body immunity due toprevious infection or vaccination, and the ability of these viruses that can be transmitted efficiently fromhuman to human through breathing, direct contact, and through items that have been touched by sufferersof seasonal influenza. Vaccination is the most effective method for controlling the infection of seasonalinfluenza and the most important strategy for pandemic prevention and control. The ideal vaccine mustimprove the humoral and cellular immune response to reduce morbidity and mortality. This study wasconducted to determine the role of trivalent influenza vaccination against the response of ferret antibodiesby testing IgG levels and IL-4 levels. IgG is an antibody formed in response to vaccination, whereas IL-4is a cytokine that stimulates B cells to differentiate into plasma cells and produce antibodies. The methodof this study is an experimental laboratory. Twenty five ferrets separated into 5 group, control, trivalentvaccine dose 3.8 µg, dose 7.5 µg, dose 15 µg, and dose 30 µg. Ferrets were vaccinated with trivalentvaccine, intramuscular, 3 weeks later, that were challenge by H1N1, H3N2 and by influenza H1, H3 dan B(wild subtype) virus. On day 35th serum was taken and examined for IgG and IL-4. The level of IgG andIL-4 was measured by Elisa. We used SPSS 23 for data analysis. From the results of the research conductedenhancement in specific levels of IgG was obtained against H1 and H3 antigen at dose of 3.8 ?g and 7.5 ?g.There is no change in specific levels of IgG against By antigen. There is no change in IL-4 levels. Therefore,we conclude that IgG and IL-4 values can be used as biomarkers in testing influenza vaccines.

Antibacterial Effects of Fluoride in Streptococcus mutans Growth in Vitro Yura Pradiptama; Marijam Purwanta; Harianto Notopuro
Biomolecular and Health Science Journal Vol. 2 No. 1 (2019): Biomolecular and Health Science Journal
Publisher : Faculty of Medicine, Universitas Airlangga, Surabaya, Indonesia

Introduction: Streptococcus mutans is a gram-positive coccus commonly found in the human oral cavity and is a pathogen of dental caries. S. mutans known to form biofilm in infective endocarditis heart-valve. Fluoride usage known to reduce the risk of dental caries. This study aims to analyze how usage of fluoride in inhibiting S. mutans growth.Methods: This study was an experimental study. Antibacterial activity test was performed to evaluate the minimum inhibitory concentration (MIC) using dilution method of sodium fluoride. The minimum bactericidal concentration (MBC) was determined by culturing from the previous dilution test into Chocolate Agar Plate.Results: MIC for sodium fluoride is 4,8 mg/ml and the MBC for sodium fluoride to S. mutans is 4,8 mg/ml. We found S. mutans growth in higher concentration than 19,2 mg/ml. Conclusion: S. mutans was inhibited in dilution test. Growth of the bacteria in higher concentration of sodium fluoride is explained with quasi-irreversible inhibtior effects of fluorida.

The Effect of Cigarette Smoke Exposure in the Fetal Growth and Fetal Development of Mice (Mus musculus) Endy Novryan Ridwan; Martono Tri Utomo; Harianto Notopuro
JUXTA: Jurnal Ilmiah Mahasiswa Kedokteran Universitas Airlangga Vol. 11 No. 1 (2020): Jurnal Ilmiah Mahasiswa Kedokteran Universitas Airlangga
Publisher : Faculty of Medicine Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/juxta.V11I12020.13-17

Introduction: This research aims to investigate and observe the effect of cigarette smoke exposure in the fetal growth and fetal development of mice (Mus musculus). Methods: This was an experiment with post-test only control group design. The sample of the research was 36 pregnant mice which were randomly divided into 2 groups: control group (K) pregnant mice which inhaled ambient air without cigarette smoke exposure, and treatment group (P) pregnant mice which were given cigarette smoke exposure for 14 days with 2 bars of cigarette each day. Results: The results showed a significant difference in the fetal birth weight between the group exposed to cigarette smoke (p < 0.05) compared with the control group. Fetal defect and stillbirth were not found in this research. Conclusion: The exposure of cigarette smoke gave negative effects of fetal growth and development because of the free radicals generated.

PENGARUH ASAM ASKORBAT TERHADAP KADAR TIMBAL FETUS DAN AKTIVITAS ENZIM SITOKROM P450 1A1 (CYP1A1) PADA INDUK MENCIT TERINTOKSIKASI TIMBAL Juliana Christyaningsih; Harianto Notopuro; Win Darmanto; Diah Titik Mutiarawati
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 16 No 1 (2010): December 2010
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23869/280

Lead contamination occurs through air pollution and industry, enter the body through respiratory and digestive tract. High lead content will accumulate and affect adversely the cognitive function, causing neuropsychological dysfunction, encephalopathy, hyperactivity and other problems in children, disrupt the central nervous system and the immune system of children as well. This experimental research was randomized control group post-test only design. The experiment used of 27 pregnant mice, divided into three groups: negative control group, which were given distilled water, positive control group were exposed only to lead and the third group were exposed to lead and administered ascorbic acid. 25 mg/kg/day/orally neutral lead acetate was given during gestation day 7 to 16, and ascorbic acid 64 mg/kg/day/orally, started on gestation day 9 to 16. Treatment group with ascorbic acid supplementation had the lowest CYP1A1 enzyme activity compared to positive and negative control groups. This results confirmed by the molecular weight of CYP1A1 enzyme ranges 53.7 to 59.2 kDa, and the western blotting test showed the same thin band both two groups. The lowest of the average lead concentration in the head of fetal mice was found on the group of mice that treated with vitamin C. Supplementation of ascorbic acid can protect the liver and fetuses, by suspected mechanism that ascorbic acid could chelate the lead and excrete it via urine.

Co-Authors A Kentjono, Widodo Amellya Octifani Aryati Aryati Awalia Ayesie Natasha Zulka B Notopuro, Paulus Bakhtiar, Arief Chiquita Prahasanti Choirotussanijjah Djajadiman Gatot Ely Rahmatika Nugrahani Endy Novryan Ridwan Ginanjar Sasmito Adi Humairah, Ira I Dewa Gede Ugrasena Indri Safitri Indri Safitri, Indri Jemima Lewi Santoso Joewono Soeroso Juliana Christyaningsih Jusak Nugraha Kadeq Novita Prajawanti Lukitasari, Lina Martono Tri Utomo Mutiarawati, Diah Titik Ponpon Idjradinata Prabowo, Gwenny Ichsan Prawitasari, Dita Sukmaya Purwanta, Marijam Qurnianingsih, Ema Retno Handajani S.Pd. M Kes I Ketut Sudiana . Siti Khaerunnisa SOETJIPTO . Sudarmo, Subijanto Marto Susi Wahyuning Asih Suwandito Suwandito Syafa’ah, Irmi Widajati, Rahma Win Darmanto Wungu, Citrawati Dyah Kencono Yohanes Adrian Kapri Negara Yura Pradiptama Zuhrotul Eka Yulis Anggraeni

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