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Skrining Fitokimia Dan Uji Sitotoksisitas Ekstrak Etanol Biji Anggur (Vitis vinifera L.) Dengan Metode BSLT (Brine Shrimp Lethality Test) Asriningtyas, Kinanti; Nasution, M. Pandapotan
AL-MIKRAJ Jurnal Studi Islam dan Humaniora (E-ISSN 2745-4584) Vol 5 No 01 (2024): Al-Mikraj, Jurnal Studi Islam dan Humaniora
Publisher : Pascasarjana Institut Agama Islam Sunan Giri Ponorogo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37680/almikraj.v5i01.5640

Cancer is an abnormal state of cells, which is the leading cause of death worldwide after cardiovascular disease and is expected to reach 12 million deaths by 2030. There are various kinds of plants that can be used as anti-cancer treatment, one of which is grape seeds (Vitis vinifera L.). Grape seeds contain 74–78% oligometric proanthocyanidins and grape seed extract contains flavonoids. Proanthocyanidins of grape seeds are a group of polyphenols. The objective of this research was to determine the content of grape seeds and determine the level of toxicity of LC50 using the BSLT (Brine Shrimp Lethality Test) method. The research was conducted using the BSLT (Brine Shrimp Lethality Test) method by calculating the number of deaths of Artemia salina larvae with Lethal concentration parameters 50 (LC50). Grape seed extract variation, chemical content, characterization and cytotoxicity tests were variables in the research. The results of phytochemical screening testing showed that red grape seeds were positive for alkaloids, flavonoids, saponins, tannins, and steroids. The results of cytotoxicity test with probit analysis showed an LC50 value of 367.2823 μg / mL so that it can be concluded that red grape seed ethanol extract is toxic and has the potential to be anticancer.

Aktivitas Antibakteri Ekstrak Etanol Daun Ruku-Ruku (Ocimum tenuiflorum L.) Terhadap Bakteri Staphylococcus epidermidis Alaina, Nadila; Mambang, Elysa Putri; Nasution, M. Pandapotan; Nasution, Haris Munandar
BEST Journal (Biology Education, Sains and Technology) Vol 6, No 2 (2023): September 2023
Publisher : Program Studi Pendidikan Biologi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30743/best.v6i2.8107

Daun ruku-ruku (Ocimum tenuiflorum L.) merupakan salah satu tanaman obat yang dikenal masyarakat digunakan sebagai bumbu masakan karena aromanya yang dapat mengurangi bau tidak sedap. Berdasarkan kandungan kimianya, daun ruku-ruku banyak mengandung alkaloid, flavonoid, tanin, saponin dan steroid/triterpenoid. Tujuan penelitian ini adalah untuk mengetahui aktivitas antibakteri ekstrak etanol daun ruku-ruku terhadap bakteri Staphylococcus epidermidis. Metode penelitian dilakukan secara eksperimental dengan variabel bebas yaitu ekstrak etanol daun ruku-ruku dengan konsentrasi 5%, 10% dan 20% sedangkan variabel terikatnya adalah aktivitas antibakteri terhadap Staphylococcus epidermidis. Ekstrak sederhana daun ruku-ruku dibuat dengan pelarut etanol 96%. Kontrol positif yang digunakan adalah Tetrasiklin dan kontrol negatif adalah DMSO. Pengujian yang dilakukan terhadap simplisia daun ruku-ruku meliputi pemeriksaan fitokimia, pemeriksaan makroskopis, pemeriksaan mikroskopis, pemeriksaan kadar air, pemeriksaan nira larut air, pemeriksaan kadar sari larut etanol, pemeriksaan kadar abu dan pemeriksaan kadar abu tidak larut asam. Hasil skrining fitokimia menunjukkan bahwa daun ruku-ruku (Ocimum tenuiflorum L.) mengandung senyawa metabolit sekunder yaitu alkaloid, flavonoid, tanin, saponin dan streroid/triterpenoid. Hasil penelitian aktivitas antibakteri menunjukkan bahwa daun ruku-ruku dapat digunakan sebagai antibakteri karena mempunyai daya hambat yang kuat pada konsentrasi 5%, 10% dan 20% yaitu 8,7 mm, 9,8 mm dan 12,1 mm terhadap bakteri Staphylococcus epidermidis

Determination of Total Flavonoid Content of Ethanol Extract and Ethyl Acetate Fraction of Avocado Seed (Persea americana Mill) by Visible Spectrofotometry Methods Natasya, Siti Adinda; Nasution, M. Pandapotan; Nasution, Haris Munandar; Yuniarti, Rafita
Indonesian Journal of Science and Pharmacy Vol. 2 No. 3 (2025): Indonesian Journal of Science and Pharmacy
Publisher : Pustaka Media Publishing

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.63763/ijsp.v2i3.98

Avocado (Persea americana Mill.) has long been used in traditional medicine, and its seeds are known to contain flavonoid compounds, which are natural phenolics with antioxidant properties and potential therapeutic benefits. This study aims to identify the chemical compounds present in the ethanol extract and ethyl acetate fraction of avocado seeds, and to determine their total flavonoid content using UV-Visible spectrophotometry. This experimental study included sample collection, simplicia characterization, extraction, fractionation, phytochemical screening, and flavonoid quantification. Phytochemical screening showed that the ethanol extract contains alkaloids, flavonoids, tannins, saponins, triterpenoids, and glycosides, while the ethyl acetate fraction contains alkaloids, flavonoids, tannins, steroids, and glycosides. Flavonoid content was determined using quercetin as a standard. The results showed that the total flavonoid content in the ethanol extract was 8.655 ± 0.144 mgQE/g, while the ethyl acetate fraction contained 32.828 ± 0.105 mgQE/g. It can be concluded that the ethyl acetate fraction contains a significantly higher level of flavonoids compared to the ethanol extract.

FORMULASI DAN UJI AKTIVITAS DEODORANT STICK EKSTRAK ETANOL DAUN KEMANGI (OCIMUM BASILICUM L) TERHADAP BAKTERI STAPHYLOCOCCUS AUREUS Selvicahyani, Selvicahyani; Nasution, M. Pandapotan; Yuniarti, Rapita; Dalimunthe, Gabena Indrayani; Lubis, Minda Sari
Jurnal Kesehatan Tambusai Vol. 6 No. 3 (2025): SEPTEMBER 2025
Publisher : Universitas Pahlawan Tuanku Tambusai

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31004/jkt.v6i3.34501

Daun kemangi (Ocimum basilicum L) merupakan tanaman yang memiliki potensi sebagai antimikroba karena mengandung senyawa metabolit sekunder seperti alkaloid, flavonoid, saponin, tanin, triterpenoid, dan glikosida. Penelitian ini bertujuan untuk mengetahui potensi ekstrak etanol daun kemangi dalam formulasi deodorant stick sebagai sediaan yang memiliki aktivitas antibakteri terhadap Staphylococcus aureus. Metode penelitian dimulai dengan pengambilan sampel daun kemangi secara purposif dari daerah Kampar, Provinsi Riau. Sampel dikeringkan untuk dijadikan simplisia, kemudian diekstraksi menggunakan metode maserasi dengan pelarut etanol 96% dan diuapkan menggunakan rotary evaporator. Skrining fitokimia dilakukan untuk mengetahui kandungan senyawa aktif dalam simplisia. Ekstrak kemudian diformulasikan dalam bentuk deodorant stick dengan konsentrasi 1%, 2%, dan 3%, serta dibandingkan dengan blangko dan produk komersial. Evaluasi sediaan meliputi uji organoleptis, homogenitas, pH, waktu leleh, waktu lebur, serta daya hambat terhadap bakteri Staphylococcus aureus. Hasil penelitian menunjukkan bahwa ekstrak daun kemangi mengandung berbagai senyawa metabolit sekunder dan simplisia memenuhi persyaratan mutu MMI 5. Formulasi deodorant stick dengan ekstrak etanol daun kemangi pada konsentrasi 3% menunjukkan daya hambat paling tinggi dengan diameter rata-rata 14,5 mm dan tergolong kategori kuat. Dengan demikian, ekstrak daun kemangi berpotensi digunakan sebagai bahan aktif alami dalam produk deodorant antimikroba.

Skrining Fitokimia Dan Uji Sitotoksisitas Ekstrak Etanol Biji Anggur (Vitis vinifera L.) Dengan Metode BSLT (Brine Shrimp Lethality Test) Asriningtyas, Kinanti; Nasution, M. Pandapotan
AL-MIKRAJ Jurnal Studi Islam dan Humaniora Vol. 5 No. 01 (2024): Al-Mikraj, Jurnal Studi Islam dan Humaniora
Publisher : Pascasarjana Institut Agama Islam Sunan Giri Ponorogo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37680/almikraj.v5i01.5640

Cancer is an abnormal state of cells, which is the leading cause of death worldwide after cardiovascular disease and is expected to reach 12 million deaths by 2030. There are various kinds of plants that can be used as anti-cancer treatment, one of which is grape seeds (Vitis vinifera L.). Grape seeds contain 74–78% oligometric proanthocyanidins and grape seed extract contains flavonoids. Proanthocyanidins of grape seeds are a group of polyphenols. The objective of this research was to determine the content of grape seeds and determine the level of toxicity of LC50 using the BSLT (Brine Shrimp Lethality Test) method. The research was conducted using the BSLT (Brine Shrimp Lethality Test) method by calculating the number of deaths of Artemia salina larvae with Lethal concentration parameters 50 (LC50). Grape seed extract variation, chemical content, characterization and cytotoxicity tests were variables in the research. The results of phytochemical screening testing showed that red grape seeds were positive for alkaloids, flavonoids, saponins, tannins, and steroids. The results of cytotoxicity test with probit analysis showed an LC50 value of 367.2823 μg / mL so that it can be concluded that red grape seed ethanol extract is toxic and has the potential to be anticancer.

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