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All Journal Jurnal Analis Medika Biosains (JAMBS) Human Care Journal Quagga: Jurnal Pendidikan dan Biologi Jurnal Kesehatan Cendikia Jenius
Ikhsan Mujahid
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Biochemistry, Genetics & Molecular Biology Education Health Professions Immunology & microbiology Medicine & Pharmacology Nursing Public Health Veterinary

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Potensi Air Kelapa Muda Dan Air Kelapa Obat Terhadap Pertumbuhan Bakteri Methicillin-Resistant Staphylococcus Aureus (MRSA) Dengan Metode Dilusi Finda Rizky Putri Prabowo; Ikhsan Mujahid; Arif Mulyanto
Jurnal Analis Medika Biosains (JAMBS) Vol 8, No 2 (2021): JURNAL ANALIS MEDIKA BIOSAINS (JAMBS)
Publisher : Poltekkes Kemenkes Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32807/jambs.v8i2.235

Abstract

Methicillin-Resistant Staphylococcus aureus merupakan strain S. aureus yang telah resisten terhadap aktivitas antibiotik golongan β-laktam, termasuk golongan penicillinase-resistant penicillins (oxcacillin, methicillin, nafcillin, cloxacillin, dicloxacillin), cephalosporin dan carbapenem. Kelapa yang sering dimanfaatkan sebagai obat yaitu kelapa muda kulit hijau dan kelapa obat atau sering disebut dengan kelapa wulung yang ditandai dengan bagian mesokarp (sabut) berwarna merah muda. Kelapa wulung memiliki khasiat untuk mengobati berbagai jenis penyakit. Tujuan penelitian ini adalah untuk mengetahui kemampuan air kelapa muda dan air kelapa obat dalam mempengaruhi pertumbuhan bakteri Methicillin-Resistant Staphylococcus aureus (MRSA). Penelitian ini dilakukan secara true eksperimental dengan rancangan pre test post test control group design. Terdiri dari 6 kelompok dengan 2 kelompok sebagai kontrol positif dan kontrol negatif serta 4 kelompok diberi perlakuan air kelapa muda kulit hijau, air kelapa muda kulit coklat, air kelapa obat kulit hijau, dan air kelapa obat kulit coklat. Jumlah bakteri sebelum dan sesudah perlakuan dihitung secara langsung menggunakan haemocytometer dan tidak langsung ditumbuhkan pada medium MHA. Penelitian ini dilakukan dari bulan Juli-Oktober 2020. Data dianalisis dengan uji repeated ANOVA. Hasil penelitian menunjukkan adanya pengaruh air kelapa terhadap jumlah bakteri MRSA dengan metode langsung, metode tidak langsung, dan perbandingan antara metode langsung dan tidak langsung. Air kelapa obat kulit coklat paling baik dalam menghambat pertumbuhan bakteri MRSA dengan penurunan jumlah bakteri 8,2 x 105 ± 5,27 x 105 CFU/mL sebelum perlakuan menjadi 1,7 x 105 ± 9,01 x 104CFU/mL setelah perlakuan pada metode tidak langsung.
EFEKTIFITAS JUS JERUK DAN JUS TOMAT TERHADAP HIPERTENSI DI DESA TAMBAKSOGRA BANYUMAS Rani Marlina; Ikhsan Mujahid
HUMAN CARE JOURNAL Vol 5, No 3 (2020): Human Care Journal
Publisher : Universitas Fort De Kock

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32883/hcj.v5i3.760

Abstract

Latar Belakang: Hipertensi atau Peningkatan tekanan darah yang berlangsung dalam jangka waktu lama menimbulkan kerusakan pada ginjal, jantung, dan otak bila tidak dideteksi secara dini dan mendapat pengobatan yang memadai.Tujuan: Menganalisa efektifitas jus jeruk dan jus tomat terhadap penurunan tekanan darah pasien hipertensi Metode: Penelitian ini menggunakan metode kuantitatif desain Quasy Eksperimen dengan rancangan two group pre-test post-test design teknik purposive sample yaitu 15 responden jus jeruk dan 15 jus tomat. Uji statistik Independent sampel t-test.Hasil: Hasil penelitian rata-rata tekanan darah sistolik jus tomat sebelum 155,60±3.54159 dan setelah 130.20±6.57050. rata-rata tekanan diastolik sebelum diberikan jus tomat adalah 97,26±2.15362 dan setelah 81.33±4.08248. Dan rata-rata tekanan sistolik sebelum diberikan jus jeruk adalah 155,46±3.46135 dan setelah 137.26±4.09646. Tekanan diastolik mean sebelum diberikan jus jeruk adalah 97.86±1.24595 dan setelah 87.80±2.27408.Kesimpulan : Terdapat perbedaan signifikan rata-rata sistolik dan diastolik kedua kelompok terhadap perubahan tekanan darah pasien hipertensi dengan nilai t hitung sistolik 3.820 dan diastolik 4.482 atau t hitung > t table dan p-value <0.05. Jus tomat lebih efektif dibandingkan dengan jus jeruk untuk menurunkan tekanan darah
Relationship Long Time Storage of Trunk Fish (Euthnymus Affinis) Smoke With The Presence Of Staphylococcus Aureus Bacteria At Bobotsari Traditional Market Zakiyatudin Anduko Romadhon; Arif Mulyanto; Ikhsan Mujahid; Oei Stefani Yuanita Widodo
Jurnal Analis Medika Biosains (JAMBS) Vol 10, No 2 (2023): JURNAL ANALIS MEDIKA BIOSAINS (JAMBS)
Publisher : Poltekkes Kemenkes Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32807/jambs.v10i2.325

Abstract

Fresh fish can experience spoilage due to the activity of enzymes and microorganisms contained in the body of the fish itself due to the oxidation process in body fat by air. The body of the fish contains water which is quite high, namely 60-80% and has a pH of 7.2 so that it can be a good medium for the growth of putrefactive bacteria. Preservation methods are carried out to reduce the presence of spoilage alternatif preservation can be done, among others, by salting, cooling, drying, and smoking. Excess fumigation contains phenol which can function as an anti-microbial so that microbes will die and not develop. The purpose of this study was to determine the relationship between long storage of smoked tuna (Euthynmus affinis) and the presence of Staphylococcus aureus bacteria. The sample in this study were 12 samples of smoked cob fish which were sold in the traditional markets of the ballpark and the sampling technique used was random sampling. This type of research was experimentally analyzed using the Kruskall Wallis test. The results of the study of 12 samples of tuna that contained Staphylococcus aureus bacteria concluded that there was a relationship between storage duration of smoked tuna (Euthynmus affinis) and the presence of Staphylococcus aureus bacteria.
Viability of Methicillin-Resistant Staphylococcus aureus (MRSA) Bacteria Preserved at Different Temperature and Time Sekar Karima Rachma; Kurniawan; Ikhsan Mujahid; Kurnia Ritma Dhanti
Quagga: Jurnal Pendidikan dan Biologi Vol 16 No 2 (2024): QUAGGA : Jurnal Pendidikan dan Biologi
Publisher : Program Studi Pendidikan Biologi, Fakultas Keguruan dan Ilmu Pendidikan, Universitas Kuningan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25134/quagga.v16i2.60

Abstract

The bacteria Staphylococcus aureus, a normal component of human flora, is easily mutated and antibiotic-resistant, leading to the creation of Methicillin-Resistant Staphylococcus aureus (MRSA). This bacterium is a difficult-to-control nosocomial infection in healthcare settings, so it must be stored using specialized preservation agents, like DMSO, to investigate the nature and characteristics of these bacteria for prevention, control, and therapy. The purpose of this study was to evaluate the survival of MRSA bacteria that had been kept in a DMSO solution at various temperatures and periods. This study was carried out between July and October2022 using a one-shot study case research design, data analysis, and quantitative descriptive analysis. According to the findings, MRSA bacteria held in 15% DMSO solution lost viability over time and at various storage temperatures. Among various bacterial species held at various storage temperatures, MRSA bacteria had the lowest colony loss rate (-20 °C). Based on the findings of this study, it can be said that the preservation of MRSA bacteria at freezing temperature (-20 °C) and storage length of one month resulted in the greatest survivability of the bacteria when stored in 15% DMSO solution.
Antibacterial Effectiveness of Beetroot (Beta vulgaris L.) Juice as an Alternative to Eosin for the Quality of Soil-Transmitted Helminth Egg Examination Yunita Nurmalasari; Ikhsan Mujahid; Dita Pratiwi Kusuma Wardani; M. Luthfi Almanfaluthi
Jurnal Kesehatan Cendikia Jenius Vol. 3 No. 1 (2025): Desember
Publisher : CV. CENDIKIA JENIUS INDONESIA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.70920/jenius.v3i1.335

Abstract

The 2% eosin reagent is commonly used in Soil Transmitted Helminth (STH) egg examination; however, its high cost, toxicity, and poor storage stability highlight the need for safer, natural-based alternatives. This study aimed to evaluate the effectiveness of beetroot (Beta vulgaris L.) juice as a substitute for eosin in staining STH eggs. A true experimental design with a post-test only control group was employed, consisting of six groups: one positive control (2% eosin) and five treatment groups using beetroot juice at concentrations of 40%, 50%, 60%, 80%, and 100%, with 11 preparations per group. Data collection was conducted through microscopic observation in July–August 2025 and analyzed using the Kruskal-Wallis test followed by the Mann-Whitney U post hoc test. The findings revealed significant differences in staining effectiveness (p = 0.027), with the 60% and 100% concentrations showing the best clarity and completeness of egg morphology. The study concludes that beetroot juice is an effective natural alternative to eosin. Further research is recommended to assess storage stability and explore other eco-friendly natural dyes.
Co-Authors Arif Mulyanto Dita Pratiwi Kusuma Wardani Finda Rizky Putri Prabowo Ketut Tirtayasa Kurnia Ritma Dhanti Kurniawan M. Luthfi Almanfaluthi Oei Stefani Yuanita Widodo Rani Marlina Sekar Karima Rachma Yunita Nurmalasari Zakiyatudin Anduko Romadhon