Siti Bahirrah
Department Of Orthodontics, Faculty Of Dentistry, Universitas Sumatera Utara, Medan, Indonesia

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Mobe leaf (Artocarpus lakoocha Buch. Ham) ethanol extract's antibacterial activity on Streptococcus mutans cell membrane leakage and biofilm formation: An in vitro study Bahirrah, Siti; Satria, Denny; Yuwanda, Cindy
Dental Journal (Majalah Kedokteran Gigi) Vol. 56 No. 4 (2023): December
Publisher : Faculty of Dental Medicine, Universitas Airlangga https://fkg.unair.ac.id/en

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/j.djmkg.v56.i4.p226-232

Abstract

Background: Using fixed orthodontic appliances inhibits oral hygiene, which can lead to the increased development of biofilms and Streptococcus mutans, a cariogenic bacterium that is well known for causing dental caries, derived from bacteria of the Streptococcus genus. In order to decrease biofilm and the degree of cariogenic bacteria in the oral cavity, a variety of herbal ingredients are used. Among these, mobe (Artocarpus lakoocha Buch. Ham) leaves are herbal ingredients with antibacterial properties. Purpose: This study aimed to investigate the antibacterial activity, antibiofilm, and leakage of DNA and protein from mobe leaves. Methods: The diffusion method was used to assess antibacterial activity and determine the minimum inhibitory concentration. The antibiofilm activity was evaluated with Ultraviolet–visible (UV-Vis) spectrophotometry (600 nm), using violet crystal staining. The detection of DNA and protein leakage was carried out by checking for absorbance values using the UV-Vis spectrophotometry (260 nm and 280 nm). An increase in the absorbance value in the measured cell indicated an increase in the level of cell content produced by the cell. One-way analysis of variance was used statistically analyze the results of this study (P < 0.05). Results: Mobe leaf (A. lakoocha) extract's minimum inhibitory concentration level was 3.125 mg/ml, the effective concentration of ethanol extract for inhibiting biofilm formation was 3.125 mg/ml, and the effective concentration of ethanol extract that could cause DNA and protein leakage was 50 mg/ml. Conclusions: Mobe leaf extract has good MIC for S. mutans.
The Effects of Different Bracket Types on Orthodontic Treatment Evaluated with the Objective Grading System Suli Andryani; Nazruddin Nazruddin; Siti Bahirrah
Scientific Dental Journal VOLUME 3, ISSUE 1, JANUARY 2019
Publisher : Faculty of Dentistry, Universitas Trisakti

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25105/sdj.v3i1.3634

Abstract

Background: Standard edgewise and preadjusted Roth are two bracket types widely used for orthodontic treatment. Whether one of these bracket types offers better treatment results than the other requires further evaluation. The Objective Grading System created by the American Board of Orthodontics (ABO) is one of the most reliable indices used to evaluate treatment outcomes. Objectives: To determine the effects of using two different bracket types on treatment outcomes by using the Objective Grading System. Methods: The sample for this study consisted of 64 randomly selected post-treatment dental casts and panoramic radiographs. Of these samples, 32 were treated with a standard edgewise bracket, and the others were treated with a preadjusted Roth bracket. Patient samples were included if they had non-extraction Class I malocclusion (minor crowding < 4 mm for both bracket types, ANB = 2º ± 2º), no history of dental trauma, complete teeth (except third molars), and no growth or development disturbances. All samples were evaluated using eight parameters of the Objective Grading System and statistically analyzed using Mann–Whitney and chi-squared tests. The score range for each tooth in each parameter was 0 – 2. Results: The total score was 19.00 ± 12.00 for the standard edgewise bracket and 15.00 ± 7.00 for the preadjusted Roth bracket, with no statistically significant differences between the two bracket types (p = 0.149). There were also no statistically significant differences in the scores of the eight parameters of the Objective Grading System between these brackets (p > 0.05). The highest score was found for the buccolingual inclination parameter, and the lowest score was for interproximal contacts. Conclusion: According to the Objective Grading System, there are no statistically significant differences between the orthodontic treatment outcomes obtained using a standard edgewise or a preadjusted Roth bracket.