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All Journal Jurnal Penelitian dan Pengembangan Pertanian Jurnal Ilmu Ternak Veteriner WARTAZOA Indonesian Bulletin of Animal and Veterinary Sciences
Sjamsul Bahri
Research Institute for Veterinary Science Bogor 16114
Agriculture, Biological Sciences & Forestry Decision Sciences, Operations Research & Management Veterinary

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The effect of aflatoxins B1 (AFB1) on chick embryo Bahri, Sjamsul; Widiastuti, R; Mustikaningsih, Y
Indonesian Journal of Animal and Veterinary Sciences Vol 10, No 2 (2005)
Publisher : Indonesian Animal Sciences Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (298.84 KB) | DOI: 10.14334/jitv.v10i2.469

Abstract

Aflatoxins are toxic compounds which occurred in cereals especially low qualities corn and peanuts. Aflatoxins are mutagenic, teratogenic and carcinogenic. The presence of aflatoxin in food including derived food in Indonesia had been observed, however, the observation on its toxicity effect is still limited. This research was conducted to study the effect of innoculation of aflatoxin B1 (AFB1) on the development of embryonic chicken egg, mortality and hatchability. The AFB1 was innoculated 10 μl in each 5 days age embryonic egg through air sacs dosaged 0; 15,6; 31,2; 62,5; 125 and 250 ng. The results showed that hatchability of those embryos were 66, 28, 26, 16, 0 and 0% respectively for 0; 15,6; 31,2; 62,5; 125 and 250 ng innoculation of AFB1. Innoculation of AFB1 caused malformation of the embryos, malabsorbtion of the yolk egg. The weight of hatched eggs was not significantly different in each group, eventhough there was a tendency that high AFB1 innoculation will decreased the live weight.     Key Words: Aflatoxin B1, Toxicity, Chick Embryo
Serological study against transmissible gastroenteritis (TGE) virus in several area in Indonesia Sendow, Indrawati; Syafriati, Tatty; Bahri, Sjamsul; Sarosa, Antonius
Indonesian Journal of Animal and Veterinary Sciences Vol 3, No 3 (1998)
Publisher : Indonesian Animal Sciences Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (161.884 KB) | DOI: 10.14334/jitv.v3i3.114

Abstract

A number of 1,168 pig and dog sera from 8 provinces in Indonesia were tested serologically for transmissible gastroenteritis (TGE) antibodies using serum neutralisation test to detect the prevalence of TGE in Indonesia. The sera were obtained from serum bank at Research Institute for Veterinary Science, Bogor. All sera collected before 1995 were negative antibody to TGE. However, sera collected from 2 provinces Sumatera Utara and Sulawesi Utara in 1996 had antibodies against TGE virus (14.03%). Titration of reacted sera showed varied between titres of 8 to 128.   Key words: Transmissible gastroenteritis virus, serum neutralization test
Protective antibody titre against Newcastle disease in ostriches (Struthio camelus) ., Darminto; Bahri, Sjamsul; Suryana, N.
Indonesian Journal of Animal and Veterinary Sciences Vol 3, No 4 (1998)
Publisher : Indonesian Animal Sciences Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (160.691 KB) | DOI: 10.14334/jitv.v3i4.124

Abstract

The aim of this study was to define an estimated antibody titre which was considered to be protective against Newcastle disease (ND) virus infection in ostriches. Eighteen young ostriches of 4 days of age were divided into two groups each containing 9 birds. The first group was unvaccinated and the second group was vaccinated against ND virus twice at 4 and 14 days of age. Antibody titres were monitored at 1, 14, 28, 42, 56, 70 and 85 days of age by haemagglutination inhibition (HI) test. All birds were then challenged with a velogenic strain of ND virus, Ita strain, at 42 days of age. The excretion of the challenge virus were monitored daily after challenge up to the end of this experiment. Several organs such as brain, trachea, lungs and spleen were collected from died birds for re-isolation of the challenged virus. Results indicated that all unvaccinated birds succumbed to the challenged virus, except one bird that survived challenged. In contrast to the unvaccinated birds, all vaccinated birds survived challenged, except two birds with low antibody titres succumbed challenged. All birds with antibody titres of 4 (HI-log2) or greater survived challenged. All challenged birds excreted the challenged virus through out their oropharyngs. Moreover, challenged virus can be successfully re-isolated from most organs of the died birds. This study concludes that : (a) the estimated protective titre against ND in ostriches is 4 (HI-log2), (b) the immune status for ostrich with antibody titre less the 4 (HI-log2) could not be defined, and (c) vaccination against Newcastle disease in ostriches could successfully prevent birds from sick and died of ND, but unable to prevent virus infection and unable to stop carrier status after infection. Key words : Newcastle disease, ostrich, antibody, protective titre
Co-Authors Antonious Sarosa Antonius Sarosa Bess Tiesnamurti Darminto . Eny Martindah Indraningsih . Indrawati Sendow Muharam Saepulloh N. Suryana R Maryam R Widiastuti Sri Poernomo T B Murdiati T Syafriati Tatty Syafriati Y Mustikaningsih Yulvian Sani