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Cryopreservation of Aceh Swamp Buffalo (Bubalus bubalis) Semen with Combination of Glycerol and Lactose Eriani, Kartini; Sari, Nisa; Rosnizar, Rosnizar; Dasrul, Dasrul; Suhartono, Suhartono; Rizal, Muhammad
Biosaintifika: Journal of Biology & Biology Education Vol 9, No 3 (2017): December 2017
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Aceh swamp buffaloes(Bubalus bubalis) are decreasing their population and genetic quality. This study was conducted to determine the influence of lactose and glycerol cryoprotectants on spermatozoa of Aceh swamp buffaloesafterthawing.This study used completelya factorial randomized design with nine treatments, and five replications.Fresh semen of the Aceh swamp buffalowere diluted by using a combination extender lactose cryoprotectants 0 mM (L0), 60 mM (L60), 120 mM(L120) and glycerol 3% (G3), 5% (G5), 7% (G7) with the equilibration of 4 hours.The results showed that the combination of cryoprotectants L120G7 influenced significantly (P < 0.05) on the quality of spermatozoa of the Aceh swamp buffalo(B. bubalis)after thawing.The percentage of sperm motility L120G7 (42.60 1.14);viability L120G7 (55.00 0.71);acrosome integrity L120G7 (52.00 0.71); and plasma membrane integrity L120G7(53.20 1.48). The combination of lactose cryoprotectants 120 mM (L120) and glycerol 7% (G7) was the best combination to maintain the quality of spermatozoa of swamp buffalo. This finding could be used to define a policy for the spermatozoa storage of Aceh swamp buffalo to artificial insemination (AI).

UJI EFEK IMUNOSTIMULAN BUAH KURMA (Phoenix dactylifera) PADA MENCIT JANTAN (Mus musculus) GALUR BALB/C Rosnizar Rosnizar; Kartini Eriani; Iskandar Muda Ramli; Fajar Muliani
Prosiding Seminar Nasional Biotik Vol 3, No 1 (2015): PROSIDING SEMINAR NASIONAL BIOTIK III 2015
Publisher : Prosiding Seminar Nasional Biotik

Buah kurma (Phoenix dactylifera) merupakan tanaman Mediteranian yang mengandung senyawa-senyawa aktif yang diduga mempunyai efek imunostimulan. Penelitian ini bertujuan untuk mengetahui efek imunostimulan ekstrak buah kurma pada mencit jantan galur Balb/C. Penelitian ini telah dilakukan di di Laboratorium Mikroteknik Jurusan Biologi dan Laboratorium Penelitian Jurusan Kimia, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Syiah Kuala. Rancangan yang digunakan pada penelitian adalah rancangan acak lengkap (RAL) yang terdiri dari 4 perlakuan dan 5 ulangan yaitu 0 mg/kg BB (P0), 250 mg/kg BB (P1), 500 mg/kg BB (P2) dan 750 mg/kg BB (P3). Parameter penelitian yang dilakukan adalah penghitungan jumlah leukosit total dan, jumlah jenis-jenis leukosit. Hasil penelitian menunjukkan bahwa ekstrak buah kurma pada semua dosis dapat meningkatan jumlah leukosit total dan limfosit dibandingkan dengan kelompok kontrol dengan perbedaan nilai signifikasi P

IMMUNOSTIMULATORY EFFECT OF METHANOL EXTRACT OF FLAMBOYANT LEAF [Delonix regia (Boj. ex Hook.) Raf.] IN MICE Kartini Eriani; Ainsyah Ainsyah; Rosnizar Rosnizar; Yunita Yunita; Ichsan Ichsan; Al Azhar
Jurnal Natural Volume 18, Number 1, February 2018
Publisher : Universitas Syiah Kuala

Abstract. Flamboyant [Delonix regia (Boj. ex Hook) Raf.] leaf contains flavonoid compounds that are expected to have immunostimulatory effect. This research was done to determine the effect of flamboyant leaf extract on immune response by accessing the activity of immune cells and capability test the extract as immunostimulant in mice. Leaf extraction was done by maceration using methanol in the Laboratory of Biology of Chemistry Department, Faculty of Mathematics and Natural Sciences of Syiah Kuala University whereas animal treatment and testing were carried out Micro-technique Laboratory of Biology Department of the same faculty. This research used 20 male mice strain Swiss-Webster aged 7-8 weeks were randomly assigned to 4 treatment groups with five replications each. Group 1 (P0) was untreated control; group 1-3 were mice administration flamboyant leaf extract 250 mg/kg BW (P1), 500 mg/kg BW (P2), and 750 mg/kg BW (P3) per oral. The treatments were given for 14 days after one week of adaptation period. Blood samples were collected before and after extract treatment and used for leukocyte count analysis. Phagocytosis activity was accessed by carbon clearance assay on day 15. At the end of the study, all mice were sacrificed for spleen weight analysis. Data obtained was analyzed by Analysis of Variance followed by Tukey test (Leukocyte count and spleen weight) or regression analysis (carbon clearance). The results showed a flamboyant leaf extract administration resulted in increased leukocyte counts that were significantly different (p0.05) between treatment groups. Phagocytosis test indicated the extract had moderate to strong immunostimulatory effect whereas spleen weight analysis did not show any difference among treatment groups. In conclusion, flamboyant leaf methanol extract was able to increase immune cells and had potential immunostimulatory activity in mice.Keywords: Delonix regia, immunostimulant, leukocytes, lymphocyte proliferation.

MOLECULAR ANALYSIS OF PBMSP-1 GENE IN ERYTHROCYTE OF MICE INFECTED WITH Plasmodium berghei Rosnizar Rosnizar; Kartini Eriani
Jurnal Kedokteran Hewan Vol 12, No 1 (2018): March
Publisher : Universitas Syiah Kuala

This study aimed to analyze MSP-1 gene in mice erythrocyte infected with Plasmodium berghei using polymerase chain reaction (PCR). The result showed the existence of 462 bp DNA band which was assumed to encode MSP-1 protein with molecular weight of 19 kDa that can only be found in erythrocyte infected with Plasmodium berghei. BLASTn analysis showed that PbMSP-1 obtained in this study have 100% similar identity with mRNA of PbMSP-1 partial sequence (462 bp), 93% similarity with PbMSP-1 complete sequence (5750 bp and 5376 bp), and 87% similarity with PbMSP-1 incomplete sequence (333 bp).

Cryopreservation of Aceh Swamp Buffalo (Bubalus bubalis) Semen with Combination of Glycerol and Lactose Kartini Eriani; Nisa Sari; Rosnizar Rosnizar; Dasrul Dasrul; Suhartono Suhartono; Muhammad Rizal
Biosaintifika: Journal of Biology & Biology Education Vol 9, No 3 (2017): December 2017
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v9i3.11426

Aceh swamp buffaloes (Bubalus bubalis) are decreasing their population and genetic quality. This study was conducted to determine the influence of lactose and glycerol cryoprotectants on spermatozoa of Aceh swamp buffaloes after thawing. This study used completelya factorial randomized design with nine treatments, and five replications. Fresh semen of the Aceh swamp buffalo were diluted by using a combination extender lactose cryoprotectants 0 mM (L0), 60 mM (L60), 120 mM (L120) and glycerol 3% (G3), 5% (G5), 7% (G7) with the equilibration of 4 hours. The results showed that the combination of cryoprotectants L120G7 influenced significantly (P 0.05) on the quality of spermatozoa of the Aceh swamp buffalo (B. bubalis) after thawing. The percentage of sperm motility L120G7 (42.60 ± 1.14); viability L120G7 (55.00 ± 0.71); acrosome integrity L120G7 (52.00 ± 0.71); and plasma membrane integrity L120G7 (53.20 ± 1.48). The combination of lactose cryoprotectants 120 mM (L120) and glycerol 7% (G7) was the best combination to maintain the quality of spermatozoa of swamp buffalo. This finding could be used to define a policy for the spermatozoa storage of Aceh swamp buffalo to artificial insemination (AI).

IMMUNOSTIMULATORY EFFECT OF METHANOL EXTRACT OF FLAMBOYANT LEAF [Delonix regia (Boj. ex Hook.) Raf.] IN MICE Kartini Eriani; Ainsyah Ainsyah; Rosnizar Rosnizar; Yunita Yunita; Ichsan Ichsan; Al Azhar
Jurnal Natural Volume 18, Number 1, February 2018
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24815/jn.v18i1.9830

Abstract. Flamboyant [Delonix regia (Boj. ex Hook) Raf.] leaf contains flavonoid compounds that are expected to have immunostimulatory effect. This research was done to determine the effect of flamboyant leaf extract on immune response by accessing the activity of immune cells and capability test the extract as immunostimulant in mice. Leaf extraction was done by maceration using methanol in the Laboratory of Biology of Chemistry Department, Faculty of Mathematics and Natural Sciences of Syiah Kuala University whereas animal treatment and testing were carried out Micro-technique Laboratory of Biology Department of the same faculty. This research used 20 male mice strain Swiss-Webster aged 7-8 weeks were randomly assigned to 4 treatment groups with five replications each. Group 1 (P0) was untreated control; group 1-3 were mice administration flamboyant leaf extract 250 mg/kg BW (P1), 500 mg/kg BW (P2), and 750 mg/kg BW (P3) per oral. The treatments were given for 14 days after one week of adaptation period. Blood samples were collected before and after extract treatment and used for leukocyte count analysis. Phagocytosis activity was accessed by carbon clearance assay on day 15. At the end of the study, all mice were sacrificed for spleen weight analysis. Data obtained was analyzed by Analysis of Variance followed by Tukey test (Leukocyte count and spleen weight) or regression analysis (carbon clearance). The results showed a flamboyant leaf extract administration resulted in increased leukocyte counts that were significantly different (p0.05) between treatment groups. Phagocytosis test indicated the extract had moderate to strong immunostimulatory effect whereas spleen weight analysis did not show any difference among treatment groups. In conclusion, flamboyant leaf methanol extract was able to increase immune cells and had potential immunostimulatory activity in mice.Keywords: Delonix regia, immunostimulant, leukocytes, lymphocyte proliferation.

Imunogenitas Vaksin HPV dalam Pencegahan Kanker Serviks di Indonesia: Efikasi, Kebijakan, dan Hambatan Fadhliana, Nana; Malliny, Indry; Firdus, Firdus; Rosnizar, Rosnizar
Jurnal Jeumpa Vol 12 No 1 (2025): Jurnal Jeumpa
Publisher : Department of Biology Education, Faculty of Teacher Training and Education, Samudra University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33059/jj.v12i1.11681

Kanker serviks merupakan penyebab utama kematian perempuan di Indonesia dan dunia, dengan infeksi Human Papillomavirus (HPV) sebagai penyebab utama. Data Kementerian Kesehatan Indonesia menunjukkan bahwa jumlah kasus kanker serviks meningkat dari 18.1 juta kasus global pada 2018 ke 36.633 kasus di Indonesia pada 2023, dengan angka kematian mencapai sekitar 21.000 per tahun secara nasional. Vaksin HPV terbukti efektif dalam mencegah infeksi HPV onkogenik dan menurunkan angka kejadian kanker serviks. Namun, implementasi di berbagai provinsi Indonesia menunjukkan cakupan vaksinasi dan deteksi dini yang belum merata, serta angka kematian yang masih tinggi. Penelitian ini bertujuan meninjau imunogenisitas vaksin HPV, efektivitasnya dalam pencegahan infeksi HPV, serta kebijakan dan hambatan dalam pelaksanaan program vaksinasi di Indonesia. Metode yang digunakan adalah literature review dari jurnal nasional terakreditasi dan data terbaru terkait prevalensi dan program vaksinasi. Artikel yang dikaji berdasarkan topik utama yang relevan dan memiliki data primer dengan tahun publikasi antara 2014-2024. Hasil penelitian menunjukkan bahwa meskipun vaksin HPV tersedia dan digunakan di berbagai daerah di Indonesia, masih terdapat hambatan utama yang meliputi rendahnya pengetahuan masyarakat, kekhawatiran terhadap efek samping, serta kendala biaya dan logistik. Data dari provinsi menunjukkan tingkat deteksi dini berkisar 10-45%, cakupan vaksinasi 20-60%, dan angka kematian bervariasi dari 100 hingga 1.300 kasus di beberapa provinsi, sedangkan secara nasional mencapai sekitar 21.000 kasus per tahun. Kebijakan pemerintah telah mendukung distribusi vaksin, namun peningkatan edukasi masyarakat diperlukan untuk mencapai cakupan vaksin yang lebih luas dan menurunkan angka kematian akibat kanker serviks.

The immunostimulant effect of jamblang stem bark (Syzygium cumini L.) ethanol extract against mice macrophages phagocytosis activity and capacity Rosnizar, Rosnizar; sari, widya; Syahfitri, Widya; Kusuma, Hendrix Indra; safitri, novi; izzazaya, annisa
Jurnal Natural Volume 25 Number 2, June 2025
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar

This study aims to determine the effect of administering Jamblang Stem Bark Ethanol Extract (Syzygium cumini L.) (JSBEE) on the activity and phagocytosis capacity of macrophages in mice (Mus musculus) infected with Staphylococcus aureus bacteria. The research method used a completely randomized design (CRD) with five treatments and five repetitions, conducted in-vitro and in vivo test. The treatments for in vitro involved administering distilled water (T0), Stimuno (T1), and JSBEE at concentrations of 10 ppm (T2), 100 ppm (T3), and 1000 ppm (T4). Subsequently, in vivo treatment was conducted using distilled water (T0), stimuno (T1), JSBEE at 10 mg/kg (T2), 100 mg/kg (T3), and 1000 mg/kg (T4). Initially, mice underwent in vivo administration of JSBEE, administered orally via catheter tip, with a dosage of 1 mL per 10 g of body weight. JSBEE was administered orally for 10 days, followed by infection with S. aureus on the 11th day. The in vitro tests were conducted by isolating macrophage cells from the intraperitoneal fluid, to which S. aureus and JSBEE were added. Intraperitoneal fluid collected from the mice was used to prepare smears using the thin blood smear method and Giemsa staining. Macrophage phagocytosis activity was observed and assessed based on the percentage activity formula, and the phagocytic capacity of macrophages was measured by the number of S. aureus cells phagocytosed. The results indicated that JSBEE significantly affected (P0.05) both the activity and phagocytic capacity of macrophages in vivo and in-vitro. The best concentration of JSBEE for increasing both the activity value and phagocytic capacity of macrophages was 100 ppm (T3).

MOLECULAR ANALYSIS OF PBMSP-1 GENE IN ERYTHROCYTE OF MICE INFECTED WITH Plasmodium berghei Rosnizar, Rosnizar; Eriani, Kartini
Jurnal Kedokteran Hewan Vol 12, No 1 (2018): March
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v12i1.7452

This study aimed to analyze MSP-1 gene in mice erythrocyte infected with Plasmodium berghei using polymerase chain reaction (PCR). The result showed the existence of 462 bp DNA band which was assumed to encode MSP-1 protein with molecular weight of 19 kDa that can only be found in erythrocyte infected with Plasmodium berghei. BLASTn analysis showed that PbMSP-1 obtained in this study have 100% similar identity with mRNA of PbMSP-1 partial sequence (462 bp), 93% similarity with PbMSP-1 complete sequence (5750 bp and 5376 bp), and 87% similarity with PbMSP-1 incomplete sequence (333 bp).

Effect of Ethanol Extract of Sugar Apple (Annona squamosa L.) Stem Bark on Rat SGPT and SGOT Masykur Masykur; Nurdin; Lukman Hakim; Rosnizar; Widya Sari; Munira Ulfa; Novi Yana Sari; Ria Ceriana
Jurnal Penelitian Pendidikan IPA Vol 9 No 10 (2023): October
Publisher : Postgraduate, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jppipa.v9i10.4773

This study aims to compare hepatoprotector potency of the ethanol extract of sugar apple (A. squamosa) stem bark (EESSB) against the enzyme activity of SGOT and SGPT in rats induced by toxic doses of paracetamol. The method used in this study is Completely Randomized (CRD) consisting of 6 treatment groups and 3 replications. The parameters that are being observed in this study are phytochemical, antioxidant, clinical symptoms, macroscopic, SGOT and SGPT rats. Data were analyzed using Analysis of Variance (ANOVA) with a significant level of 5% with the Duncan test as a follow-up test. The clinical and macroscopic symptoms of rat liver including changes in color, surface structure and consistency as well as relative organ weight. Data were analyzed using ANOVA significant level 5% and Duncan's test as a follow-up test.Â Extracts contains alkaloid, steroid and phenolic compounds. The results showed that there was a significant difference (P <0.05) in the levels of SGOT and SGPT after giving ethanol extract of stem bark A.squamosa (EESSB). Based on the results of the study, it was concluded that all EESSB doses had the potential to reduce the levels of AST and ALT in paracetamol induced rats. The results showed that there was a significant difference (P <0.05) in the macroscopic changes in the liver based on changes in color and degree of liver damage. Relative organ weight had no significant effect (P> 0.05). The ethanol extract ofÂ A. squamosa (EESSB) stem bark has the potential to be a hepatoprotector, however a dose of 150 mg/kg BW is a more effective dose as a hepatoprotector against paracetamol.

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