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All Journal HAYATI Journal of Biosciences JSFK (Jurnal Sains Farmasi & Klinis) The Avicenna Medical Journal

CHRIS ADHIYANTO

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Author-ID : 2419328

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Earth & Planetary Sciences Health Professions Immunology & microbiology Medicine & Pharmacology Public Health

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Mutation of mtDNA ND1 Gene in 20 Type 2 Diabetes Mellitus Patients of Gorontalonese and Javanese Ethnicity AMIEN RAMADHAN ISHAK; RINI PUSPITANINGRUM; RISMA DWI UTARI; MELLA FERANIA; CHRIS ADHIYANTO; TAKENORI NITTA; AB SUSANTO; HATTORI YUKIO; YASUHIRO YAMASHIRO
HAYATI Journal of Biosciences Vol. 21 No. 4 (2014): December 2014
Publisher : Bogor Agricultural University, Indonesia

Mitochondrial gene mutation plays a role in the development of type two diabetes mellitus (T2DM). A point mutation in the mitochondrial gene Nicotinamide adenine dinucleotide dehydrogenase 1 (mtDNA ND1) gene mainly reported as the most common mutation related to T2DM. However, several studies have identified another SNP (single-nucleotide polymorphisms) in the RNA region of mtDNA from patients from specific ethnic populations in Indonesia. Building on those findings, this study aimed to use PCR and DNA sequencing technology to identify nucleotides in RNA and ND1 fragment from 20 Gorontalonese and 20 Javanese T2DM patients, that may trigger T2DM expression. The results showed successful amplification of RNA along 294 bp for all samples. From these samples, we found two types of point mutation in Javanese patients in the G3316A and T3200C points of the rRNA and ND1 gene. In samples taken from Gorontalonese patients, no mutation were found in the RNA or ND1 region. We conclude that T2DM was triggered differently in our two populations. While genetic mutation is implicated for the 20 Javanese patients, T2DM pathogenesis in the Gorontalonese patients must be traced to other genetic, environmental, or behavioral factors.

Preliminary Study in Overview of CYP2A6 Gene Variation in Infertile Male Patients Jihadin Rasyadi Mumtaz; Zeti Harriyati; Chris Adhiyanto; Nurul Hiedayati; Yona Mimanda; Muniroh Muniroh
The Avicenna Medical Journal Vol 2, No 1 (2021)
Publisher : Faculty of Medicine, UIN (State Islamic University) Syarif Hidayatullah Jakarta

Introduction: Infertility is a condition which is described by WHO as inability for having children after one year of relationship without birth control. this condition can be caused both from inside and outside the body. One of the major outside factors is exposure to chemical substances, which in this case nicotine substance that came from cigarettes. The CYP2A6 gene is a coding gene for the xenobiotic metabolic enzyme, in this case nicotine which can cause infertility in men. Mutations in this gene can lead to nicotine buildup, increasing nicotine effect thus increasing the risk of infertility in men. The aim of this study was to determine whether there were variations in the CYP2A6 gene in male infertile patients.Methods: The method used was the isolation of infertile and fertile sperm DNA samples (five samples each) which were then amplified at the target DNA (CYP2A6) by PCR method and then sequenced to see the nucleutide sequence.Result: The results of this study showed that there were variations in the CYP2A6 gene that occurred only in infertile patients, heterogeneous mutations in the 7788th(C-A), 8040th (C-T), 7661st (G-C) nucleutide sequence of the CYP2A6 gene and SNP mutations in the 8250th(T>C), 8409th (T>G/C), and 8428th(C>T) nucleutide sequence of the CYP2A6 gene.Conclusion: The conclusion of this study is that there are CYP2A6 variations that found only in infertile male patients.

Analisis Cemaran Daging Babi pada Bakso Sapi yang Dijual di Tanjung Priok menggunakan Real-Time Polymerase Chain Reaction (RT-PCR) Zilhadia Zilhadia; Chris Adhiyanto; Ayu Gustida; Nadiah Khairunnisa
Jurnal Sains Farmasi & Klinis Vol 7, No 1 (2020): J Sains Farm Klin 7(1), April 2020
Publisher : Fakultas Farmasi Universitas Andalas

Bakso sapi sebagai makanan yang disukai oleh masyarakat Indonesia rawan terhadap cemaran daging babi karena daging sapi harganya relatif mahal. Kasus pencampuran tersebut tentu menimbulkan ketidaknyamanan bagi masyarakat muslim di Indonesia karena daging babi tidak halal dikonsumsi. Karena itu perlu dilakukan analisis cemaran daging babi pada bakso sapi. Sampel diambil dari wilayah Tanjung Priuk karena jumlah penganut yang boleh mengkonsumsi daging babi di daerah tersebut signifikan banyaknya. Bakso kontrol (100% babi, 100% sapi, campuran 50% babi) dan 10 bakso sampel di analisis menggunakan metode real-time polymerase chain reaction (RT-PCR). Isolat DNA yang digunakan adalah DNA mitokondria dengan daerah target sitokrom B. Hasil amplifikasi RT-PCR pada 3 bakso kontrol menggunakan primer babi menunjukkan bakso kontrol 100% babi dan campuran 50% babi menghasilkan kenaikan kurva amplifikasi dengan CP 22,82 dan 20,03. Sedangkan hasil amplifikasi 10 sampel bakso sapi menggunakan primer babi tidak menghasilkan kenaikan kurva amplifikasi yang menunjukkan bahwa tidak adanya DNA babi yang teramplifikasi pada produk bakso sapi tersebut.

Co-Authors AB SUSANTO AMIEN RAMADHAN ISHAK Ayu Gustida HATTORI YUKIO Jihadin Rasyadi Mumtaz MELLA FERANIA Muniroh Muniroh Nadiah Khairunnisa Nurul Hiedayati Rini Puspitaningrum RISMA DWI UTARI TAKENORI NITTA YASUHIRO YAMASHIRO Yona Mimanda Zeti Harriyati Zilhadia Zilhadia

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