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All Journal HAYATI Journal of Biosciences SIMBIOSIS Bioma : Jurnal Biologi Indonesia JURNAL PENDIDIKAN TAMBUSAI Sarwahita : Jurnal Pengabdian Kepada Masyarakat CERATA Jurnal Ilmu Farmasi Parameter Patria : Jurnal Pengabdian Kepada Masyarakat CERATA

Rini Puspitaningrum

Program Studi Biologi, Fakultas Matematika Dan Ilmu Pengetahuan Alam, Universitas Negeri Jakarta

Author-ID : 350587

Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Earth & Planetary Sciences Education Health Professions Immunology & microbiology Languange, Linguistic, Communication & Media Mathematics Medicine & Pharmacology Public Health Social Sciences Other

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PENERAPAN PROGRAM REMEDIAL DALAM PEMBELAJARAN BIOLOGI DI SEKOLAH MENENGAH ATAS Mutmainah, Mutmainah; Irnidayanti, Yulia; Puspitaningrum, Rini
Parameter Vol 27 No 2 (2015): Parameter
Publisher : Lembaga Penelitian dan Pengabdian Kepada Masyarakat

Abstract: Remedial learning can improve learning achievement of learners so as to achieve a minimum completeness criteria specified. Remedial learning is learning to be a good therapeutic or cured, from the difficulty to master certain competencies expected in learning. Remedial education in Indonesia is regulated by the Ministry of Education. This study was conducted to determine the feasibility remedial procedure, performed senior high school biology teacher in Bekasi, based Juknis made by the Education Ministry, 2010. The method used is descriptive method, with survey techniques. The result showed that, the implementation of remedial highly dependent on the condition of each school. School conditions were observed in this study can be grouped into three grade, namely grade A, B and C. The procedure implementation of remedial in senior high school Bekasi, not in accordance with the National Education Ministry, but only one school. The few things that have not been appropriate including instructional time, remedial test questions do not test any indicator incomplete, remedial tests given before students follow a remedial learning, relearning not use different methods and media. Abstrak: Pembelajaran remedial dapat meningkatkan prestasi belajar peserta didik sehingga mencapai kriteria ketuntasan minimal yang ditentukan. Pembelajaran remedial adalah belajar untuk menjadi baik terapeutik atau sembuh, dari kesulitan untuk menguasai kompetensi tertentu diharapkan dalam pembelajaran. Perbaikan pendidikan di Indonesia diatur oleh Departemen Pendidikan. Penelitian ini dilakukan untuk mengetahui kelayakan prosedur perbaikan, dilakukan guru biologi SMA di Bekasi, Juknis berdasarkan dibuat oleh Departemen Pendidikan, 2010. Metode yang digunakan adalah metode deskriptif, dengan teknik survei. Hasil penelitian menunjukkan bahwa, pelaksanaan perbaikan sangat tergantung pada kondisi masing-masing sekolah. Kondisi sekolah yang diamati dalam penelitian ini dapat dikelompokkan menjadi tiga kelas, yaitu kelas A, B dan C. Pelaksanaan Prosedur perbaikan di SMA Bekasi, tidak sesuai dengan Departemen Pendidikan Nasional, tetapi hanya satu sekolah. Beberapa hal yang belum sesuai termasuk waktu pembelajaran, pertanyaan tes remedial tidak menguji setiap indikator yang tidak lengkap, tes remedial diberikan sebelum siswa mengikuti pembelajaran remedial, belajar kembali tidak menggunakan metode dan media yang berbeda.

Myoglobin Expression in Chelonia mydas Brain, Heart and Liver Tissues RINI PUSPITANINGRUM; SEPTELIA INAWATI WANANDI; RONDANG ROEMIATI SOEGIANTO; MOHAMAD SADIKIN; DARYL ROBERT WILLIAMS; ANDREW ROBERT COSSINS
HAYATI Journal of Biosciences Vol. 17 No. 3 (2010): September 2010
Publisher : Bogor Agricultural University, Indonesia

An understanding of the underpinning physiology and biochemistry of animals is essential to properly understand the impact of anthropogenic changes and natural catastrophes upon the conservation of endangered species. An observation on the tissue location of the key respiratory protein, myoglobin, now opens up new opportunities for understanding how hypoxia tolerance impacts on diving lifestyle in turtles. The respiratory protein, myoglobin has functions other than oxygen binding which are involved in hypoxia tolerance, including metabolism of reactive oxygen species and of the vascular function by metabolism of nitric oxide. Our work aims to determine whether myoglobin expression in the green turtle exists in multiple non muscle tissues and to confirm the hypothesis that reptiles also have a distributed myoglobin expression which is linked to the hypoxiatolerant trait. This initial work in turtle hatch Chelonia mydas confirms the presence of myoglobin transcriptin brain, heart and liver tissues. Furthermore, it will serve as a tool for completing the sequence and generating an in situ hybridization probe for verifying of cell location in expressing tissues.

The aim of this research was to gain the profile of blood gasses of green turtle (Chelonia mydas) hatch. Blood gas of the green turtle was analysed after exposuring them at 28 oC and 50% of humidity for 24 hours in a pvc tube and at 34 oC under sunlight exposured with 47% of humidity for 30 minutes. The result showed the different values of blood gas contents. This result showed indication of metabolism activities and poikilothermic adaptation of green turtle hatch. This information can be used RINI PUSPITANINGRUM; SEPTELIA INAWATI WANANDI; RONDANG ROEMIATI SOEGIANTO; MOHAMAD SADIKIN
HAYATI Journal of Biosciences Vol. 18 No. 3 (2011): September 2011
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.18.3.147

Isolation and Characterization of Chelonia mydas Myoglobin MOHAMAD SADIKIN; RINI PUSPITANINGRUM; SEPTELIA INAWATI WANANDI; RONDANG ROEMIATI SOEGIANTO
HAYATI Journal of Biosciences Vol. 19 No. 2 (2012): June 2012
Publisher : Bogor Agricultural University, Indonesia

Green sea turtle – Chelonia mydas is a lung respiration animal that is able to dive and stay uder sea water for hours without needing to surface for oxygen. As oxygen supply in muscles is assured by myoglobin, we propose to study some characteristic of this muscle protein in green sea turtles. To achieve this objective, pure green sea turtle myoglobin has to be made available. Therefore, our first task is to purify this muscle protein from green sea turtles. Skeletal muscles from 3 green sea turtle hatchlings were studied microscopically and biochemically. Microscopy observation showed a general structure of striated muscle. Biochemical studies revealed that green sea turtle myoglobin could be more purely isolated to a certain degree by Sephadex G-75 gel filtration and purified by immunoaffinity gel chromatography rather than direct purification by DEAE-Sepharose ion exchange chromatography technique. SDS-PAGE analysis showed that green sea turtle myoglobin ran together with horse myoglobin as 17 kDa molecular weight proteins

Comparison of Hemoglobins from Various Subjects Living in Hypoxia RINI PUSPITANINGRUM; KURNIA NUZTIR MANTOLINI; . RUSDI; MOHAMAD SADIKIN
HAYATI Journal of Biosciences Vol. 20 No. 3 (2013): September 2013
Publisher : Bogor Agricultural University, Indonesia

The aim of this research was to obtain the different characteristics of haemoglobin molecules in subjects under hypoxic condition, namely eel, catfish, suckermouth fish, green sea turtle using an electrophoresis technique. We used human umbilical cord blood and thalassemia patient blood, as well as a normal adult-human blood as controls. The proteins obtained after electrophoresis process were stained with two different colouring techniques, each based on different principles. Both staining techniques gave practically identical results. Subject that live in hypoxic condition has a different haemoglobin in comparison to the one found in adult human live in normal oxygen condition (normoxia). These hypoxia-adapted or -needed hemoglobin migrate slower than adult human hemoglobin from normoxia. This observation suggests that hemoglobin which is needed to live in hypoxic condition or environment is a different molecule. Whether this hemoglobin from hypoxic condition has a higher affinity to oxygen is not yet known.

Mutation of mtDNA ND1 Gene in 20 Type 2 Diabetes Mellitus Patients of Gorontalonese and Javanese Ethnicity AMIEN RAMADHAN ISHAK; RINI PUSPITANINGRUM; RISMA DWI UTARI; MELLA FERANIA; CHRIS ADHIYANTO; TAKENORI NITTA; AB SUSANTO; HATTORI YUKIO; YASUHIRO YAMASHIRO
HAYATI Journal of Biosciences Vol. 21 No. 4 (2014): December 2014
Publisher : Bogor Agricultural University, Indonesia

Mitochondrial gene mutation plays a role in the development of type two diabetes mellitus (T2DM). A point mutation in the mitochondrial gene Nicotinamide adenine dinucleotide dehydrogenase 1 (mtDNA ND1) gene mainly reported as the most common mutation related to T2DM. However, several studies have identified another SNP (single-nucleotide polymorphisms) in the RNA region of mtDNA from patients from specific ethnic populations in Indonesia. Building on those findings, this study aimed to use PCR and DNA sequencing technology to identify nucleotides in RNA and ND1 fragment from 20 Gorontalonese and 20 Javanese T2DM patients, that may trigger T2DM expression. The results showed successful amplification of RNA along 294 bp for all samples. From these samples, we found two types of point mutation in Javanese patients in the G3316A and T3200C points of the rRNA and ND1 gene. In samples taken from Gorontalonese patients, no mutation were found in the RNA or ND1 region. We conclude that T2DM was triggered differently in our two populations. While genetic mutation is implicated for the 20 Javanese patients, T2DM pathogenesis in the Gorontalonese patients must be traced to other genetic, environmental, or behavioral factors.

MYOGLOBIN CONTENT IN CARDIAC MUSCLE OF PHYSICAL EXERCISE INDUCED RAT (Rattus norvegicus) Sri Rahayu; Rini Puspitaningrum; Mohammad Sadikin
SIMBIOSIS Vol 4 No 2 (2016)
Publisher : Jurusan Biologi Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (724.305 KB)

ABSTRACT The research was aimed to analyzing the impact of 7 days arobic exercise in elevating rat cardiac muscle myoglobin. Experimental in vivo study on 20 male Wistar rat induced by 20m/ min treadmill aerobic exercise for 1, 3 and 7 days. Research was done in Animal house and protein laboratory, Biochemistry and Molecular Biology Department, FK UI and laboratory of Biochemistry and Molecular Biology, Biology Department FMIPA UNJ for 10 months. Myoglobin concentration was analyzed by spectrofotometry techique based on myoglobin Soret absorbance in ?503nm. Myoglobin protein expression was assesed with Western blot technique. Standard curve measurement on myoglobin content with horse myoglobin shown that there was an increase on the absorbance value. The result showedanthat the standar curve was able to be used as standard curve to calculate myoglobin concentration on cardiac muscle within 503 nm. Spectrophotometry myoglobin concentration measurement on Soret absorbance has shown increasing concentration on myoglobin since the first treatment day. Mean myoglobin concentration value has increased on the 3rd and 7th day, with highest value on the 7th treatment day (0,0656 ± 0,0001). Western blot analysis showed ng on darker band on treatment group compared on to control. When aerobic exercise was given longer, the band shown darker means that myglobin content was higher. Myoglobin concentration can be measured by spectrophotometry on 503 nm. Aerobic physical exercise increased myoglobin on rat cardiac muscle since the first treatment day and still increase oduring the treatment day. Highest level was shown on the 7th treatment day. Keywords : myoglobin, cardiac, muscle, exercise, rat

INOVASI ANEKA PANGAN BERGIZI TINGGI DARI BAHAN KEDELAI IRADIASI GAMASUGEN UNTUK MENINGKATKAN PENDAPATAN MASYARAKAT RAWAMANGUN JAKARTA TIMUR Elsa Lisanti; Rini Puspitaningrum; Nur Eka Tresnawati; Arwin
Bahasa Indonesia Vol 18 No 01 (2021): Sarwahita : Jurnal Pengabdian Kepada Masyarakat
Publisher : Lembaga Penelitian dan Pengabdian Kepada Masyarakat

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21009/sarwahita.181.8

Soybeans (Glycine max) are among the seeds that contain the highest protein of many types of nuts. Gamasugen irradiated soybeans are soybeans that can be relied on to overcome protein deficiency in the diet of the Indonesian people. The purpose of this activity is to develop various Gamasugen irradiated soybean preparations into products that can be used by the wider community in general and the community of RW 01 in particular. RW 01 on Jl. Pemuda Rawamangun is a densely populated area located in front of the Universitas Neger Jakarta. This community empowerment program was carried out on September 12, 2020, with the limited number of participants, due to the Covid-19 pandemic outbreak. The method used is the delivery of material using banners, distributing brochures, and bringing the processed Gamasugen soybean products and uploading them on You Tube. Even though with the limited conditions, the training went well. Based on results of the pre-test and post-test, it appears that there was an increase in public, the average value of the pre-test was 65 to 89 during the post-test.The result of this program showed an increase in the knowledge and skills of RW 01 community to utilize the potential of soybeans as a highly nutritious processed food from Gamasugen soybeans. Abstrak Kedelai (Glycine max) termasuk biji-bijian yang mengandung protein paling tinggi dari banyak macam jenis kacang. Kedelai iradiasi Gamasugen merupakan kedelai yang dapat diandalkan untuk mengatasi kekurangan protein dalam menu makanan rakyat Indonesia. Tujuan kegiatan ini adalah untuk mengembangkan berbagai olahan kedelai iradiasi Gamasugen menjadi produk yang dapat dimanfaatkan oleh masyarakat luas umumnya dan masyarakat RW 01 khususnya. RW. 01 di Jl. Pemuda Rawamangun merupakan kawasan lokasi padat penduduk yang terletak di depan kampus Universitas Negeri JakartaProgram Pengabdian Masyarakat ini dilakukan pada tanggal 12 September 2020, dengan jumlah peserta yang terbatas, karena adanya wabah pandemic Covid-19. Metode yang dilakukan adalah penyampaian materi dengan menggunakan banner, menyebarkan brosur, membawa hasil-hasil produk olahan kedelai Gamasugen dan mengupload di You Tube. Metode yang dilakukan adalah penyampaian materi dengan menggunakan banner, menyebarkan brosur, serta membawa hasil-hasil produk olahan kedelai Gamasugen dan mengupload di you tube. Walaupun dengan kondisi yang terbatas, pelatihan berjalan lancar. Berdasarkan hasil pre test dan pos tes tampak terjadi peningkatan pemahaman dan pengetahuan masyarakat, nilai rata-rata pre test 65 menjadi 89 pada saat post test. Hasil dari program ini menunjukkan adanya peningkatan pengetahuan dan keterampilan masyarakat RW. 01 untuk memanfaatkan potensi kedelai sebagai bahan pangan olahan yang bergizi tinggi dari kedelai Gamasugen.

Kajian Komplikasi Operasi Refraksi Mata Menggunakan Relex-Smile Risda Putri Indriani; Sudaryanti Sudaryanti; Rusdi Rusdi; Rini Puspitaningrum
Jurnal Pendidikan Tambusai Vol. 6 No. 2 (2022): Agustus 2022
Publisher : LPPM Universitas Pahlawan Tuanku Tambusai, Riau, Indonesia

RELEX SMILE menjadi pilihan alternatif selain prosedur LASIK dalam mengobati kelainan refraksi mata. Prosedur ini dinilai lebih aman dan mengurangi resiko pasca operasi. Sehingga banyak orang menggunakan prosedur ini di seluruh dunia. Namun prosedur ini bukanlah operasi mata yang tidak memiliki resiko sama sekali. Tujuan dari penelitian ini adalah untuk mendeskripsikan komplikasi yang terjadi pada prosedur RELEX SMILE. Metode penelitian berupa studi literatur dari beberapa artikel ilmiah internasional dan nasional yang diterbitkan tahun 2012-2022. Hasil penelitian didapatkan beberapa komplikasi yang terjadi selama prosedur yaitu suction loss, opaque bubble layer, bintik hitam, mata kering, ekstasia kornea, diffuse lamellar keratitis, induksi higher-order aberration, transient light sensitivity syndrome, dan kesalahan bias. Komplikasi tersebut dapat dicegah dengan skrining yang menyeluruh, pemberian informasi yang komperhensif pada pasien, ahli yang berpengalaman, disiplin dalam pengobatan dan kontrol pasca operasi.

STUDI LITERATUR DAMPAK KORTIKOSTEROID PADA WANITA HAMIL DAN PERTUMBUHAN JANIN Reza Dino Mahardika; Feni Oetari; Rini Puspitaningrum
CERATA Jurnal Ilmu Farmasi Vol 13 No 1 (2022): Cerata Jurnal Ilmu Farmasi
Publisher : UNIVRSITAS MUHAMMADIYAH KLATEN

Show Abstract | Download Original | Original Source | Check in Google Scholar

Kortikosteroid yang dibuat secara sintetis berperan penting dalam dunia medis. Kortikosteroid digunakan dalam pengobatan antiinflamasi, penyakit kulit dan autoimunitas. Penggunaan kortikosteroid pada penulisan ini dibatasi pada wanita hamil. Wanita hamil dan janin yang dikandung sangat rentan terhadap obat kortikosteroid. Efek kortikosteroid yang dapat meningkatkan tekanan darah beresiko menyebabkan preeklampsia pada wanita hamil. Selain itu kortikosteroid berpotensi mengganggu proses fisiologis plasenta. Meskipun demikian, obat kortikosteroid memiliki dampak positif terhadap wanita hamil, seperti meningkatkan pembentukan surfaktan dan perkembangan paru-paru pada janin.

Co-Authors . RUSDI AB SUSANTO Ade Suryanda Adisyahputra Adisyahputra, Adisyahputra Ali Djamhuri Amelia, Ria AMIEN RAMADHAN ISHAK ANDREW ROBERT COSSINS Arwin Auliya, Nailul Rahmi CHRIS ADHIYANTO DARYL ROBERT WILLIAMS Dewahrani, Yulilina Retno Dino Mahardika, Reza Elsa Lisanti Febrianti, Isrina Feni Oetari HATTORI YUKIO Indriani, Risda Putri KURNIA NUZTIR MANTOLINI Lestari, Amanda Putri MELLA FERANIA MOHAMAD SADIKIN MOHAMAD SADIKIN MOHAMAD SADIKIN Mohamad Sadikin Mohammad Sadikin Murtiati, Tri Nur Eka Tresnawati Oetari, Feni Reza Dino Mahardika Risda Putri Indriani RISMA DWI UTARI RONDANG ROEMIATI SOEGIANTO RONDANG ROEMIATI SOEGIANTO RONDANG ROEMIATI SOEGIANTO Rusdi Rusdi Rusdi Rusdi SEPTELIA INAWATI WANANDI SEPTELIA INAWATI WANANDI SEPTELIA INAWATI WANANDI Sri Rahayu Sudaryanti Sudaryanti Sudaryanti Sudaryanti TAKENORI NITTA YASUHIRO YAMASHIRO YULIA IRNIDAYANTI

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