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All Journal Journal of Food and Pharmaceutical Science VALENSI JSFK (Jurnal Sains Farmasi & Klinis) Pharmaceutical And Biomedical Sciences Journal (PBSJ) Pharmaceutical Sciences and Research (PSR)

Zilhadia Zilhadia

Pharmacy Department Faculty Of Health Sciences Syarif Hidayatullah State Islamic University Jakarta

Author-ID : 4268097

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemistry Health Professions Immunology & microbiology Medicine & Pharmacology Public Health

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Protein Profilesof Beef (Bos indicus), Pork (Sus domesticus),and SausagesBy Using SDS-PAGE (Sodium Dodecyl Sulphate Polyacrylamide Gel Electrophoresis) Method Zilhadia, Zilhadia
Journal of Food and Pharmaceutical Sciences Vol 2, No 2 (2014): J. Food Pharm. Sci (May-August)
Publisher : Fakultas Farmasi, Universitas Gadjah Mada

A research has been done to analyze the protein profile in fresh beef, fresh pork, and 10 beef sausage by using SDS PAGE (Sodium Dodecyl Sulphate Polyacrylamide Gel Electrophoresis) with 2 plate gel electrophoresis. From this research, we found several protein bands that become distinctive protein bands. On raw beef protein we found three bands that are not found in pork. They are protein band with molecular weight (MW) of 144,54 kDa, 81,28 kDa and 58,88 kDa respectively. On the raw pork, we found 5 protein bands that are not found in raw beef, namely protein bands with MW 154,88 kDa; 146,55 kDa; 83,18 kDa; 69,18 kDa and 61,66 kDa. There is a band on pork protein found on the second plate on MW gel is 69,18 kDa. Whereas in 10 samples of beef sausages we did not found any specific protein bands. This is presumably due to the difference in manufacturing process performed by the manufacturer.

Determination Of Formaldehyde In Tofu From Ciputat Traditional Market With Colorimetry Method Zilhadia Zilhadia; Sanny Susanti
Jurnal Kimia Valensi Jurnal Valensi Volume 2, No.2, Mei 2011
Publisher : Syarif Hidayatullah State Islamic University

Tofu, a food product made from soybean, contain water and it is very easy to overgrown microbes.Based on a survey, many products of tofu contain formaldehyde as preservative. Formaldehyde is apreservative that the use is prohibited for food because it can cause cancer in humans. The aim of thisstudy was determined concentration of formaldehyde in tofu from traditional market Ciputat usingSpectrophotometer UVI-VIS. Steam distillation was used for sample extraction process. Distillate wasreacted with Nash reagent. The equation of calibration curve is y=0,0032x – 0,0079 and correlationcoefficient of the linear regression of 0,9992. The experimental results obtained LOD value of11.1328 μg/ml, LOQ value of 37,1094 μg/mL The precision of this analytical method were lower than2% for each of the sample, while method’s accuration for tofu was 98,69% ± 0,4085%. Results ofsample analysis from Ciputat market, samples were detected containing formaldehyde. Formaldehydeconcentrations are 104,87 μg/mL, 11,21 μg/mL, 1,96 μg/mL, 190,80 μg/mL, 201,98 μg/mL, 10,47μg/mL, and 3,31 μg/mL.

Aplikasi Metode SDS-PAGE (Sodium Dodecyl Sulphate Poly Acrylamide Gel Electrophoresis) untuk Mengidentifikasi Sumber Asal Gelatin pada Kapsul Keras Sandra Hermanto; Fahrur Rahman Saputra; Zilhadia .
Jurnal Kimia Valensi Jurnal Kimia VALENSI Volume 1, No. 1, Mei 2015
Publisher : Syarif Hidayatullah State Islamic University

Gelatin as the main ingredient of capsules is still a problem for a moslem. Most of gelatin production remains largely derived from non-halal materials. One of gelatin source is came from collagen of the skin and bones of bovine or pork. The main of study is determine the source of gelatin used in hard capsules by using SDS-PAGE (Sodium Dodecyl Sulphate Gel electrophoresis Poly Acrylamide) method. In the early stages, optimization of standards bovine and pork gelatin were hydrolyzed by pepsin at pH 4.5 and 60°C for 1 hour, 2 hours, and 3 hours. Gelatin hydrolyzateswere analyzed by SDS-PAGE to determine the optimal hydrolysis time. Identification of gelatin hydrolyzate fragments were carried by molecular weight. Hydrolysis time optimization throught applied to identify the source of hard gelatin capsules in the samples obtained from market and compared with the simulation of hard gelatin capsules. The results showed there were of specific bands of bovine gelatin with a molecular weight of 11,4 kDa; 34 kDa; 47kDa and specific bands of pork gelatin with a molecular weight of 24.7 kDa; 28 kDa; and 60 kDa. Similar results were obtained on a sample of hard capsules with bands of protein fragments that were identical to bovine gelatinstandard. Based on the results,each of the samples were tested contain of bovine gelatin respectively. DOI :http://dx.doi.org/10.15408/jkv.v0i0.3150

Simultaneous Determination of Metformin and Glimepiride in Fixed-Dose Combination Tablets Sold in Pramuka Market using Validated UV Spectrophotometric Method Zilhadia Zilhadia; Supandi Supandi; Salman Alfarisi
Jurnal Sains Farmasi & Klinis Vol 8, No 3 (2021): J Sains Farm Klin 8(3), Desember 2021
Publisher : Fakultas Farmasi Universitas Andalas

Combination of metformin and glimepiride as a diabetes mellitus drug has recently shown significant increase due to its benefits in improving patient compliance and offering cost effectiveness. This study aimed at analyzing metformin-glimepiride in fixed dose combinations purchased at Pramuka Market (the largest drug market in Jakarta) using validated simultaneous UV-spectrophotometry. The maximum wavelength of metformin and glimepiride is 237 nm and 228 nm respectively. The validation result of metformin and glimepiride at concentration range of 2-8 µg/mL and 5-17 µg/mL showed Linearity with 0.9999 and 0.9996, LOD values with 0.0962 µg/mL and 0.2954 µg/mL, LOQ values with 0.3208 µg/mL and 0.9846 µg/mL, and recovery (accuracy test) with 99.72% ± 1.88% to 103.86 ± 0.87%, respectively. The Relative Standard Deviation (RSD) as the precision test indicator for both drugs was less than 2%. The level of Metformin-glimepiride fixed-dose combination purchased at Pramuka Market was about 96.15% ± 3.56% to 102.71% ± 1.50%. The conclusion of this study showed that the level of metformin-glimepiride sold in the Pramuka market met the requirements

Perbandingan Metode SYBR Green dan Hydrolysis Probe dalam Analisis DNA Gelatin Sapi dan Gelatin Babi Menggunakan Real Time Polymerase Chain Reaction Zilhadia Zilhadia; Afifah Nurul Izzah; Ofa Suzanti Betha
Jurnal Sains Farmasi & Klinis Vol 4, No 1 (2017): J Sains Farm Klin 4(1), November 2017
Publisher : Fakultas Farmasi Universitas Andalas

Gelatin has a large application in the food, pharmaceutical and cosmetics industries. Gelatin is mostly derived from the skin or bone of porcine and bovine. Porcine gelatin is forbidden for Muslim and Jews. For this reason, analytical methods to detect gelatin are needed to make sure the source of gelatin. One of the analytical techniques that can differentiate bovine and porcine gelatin is Real Time Polymerase Chain Reaction (PCR). There are two popular methods of fluorescence dye, namely SYBR green and hydrolysis probe. This study was conducted to compare SYBR green and hydrolysis probe method in analyzing porcine and bovine gelatin DNA using Real Time PCR. The DNA was isolated by commercial kit. The obtained porcine and bovine gelatin DNA were 19.38 ng/μl and 13.63 ng/μl with purity were 1,566 and 1,573, respectively. Then, isolated DNA was analyzed by SYBR green and hydrolysis methods. SYBR green methods was done by annealing temperature of 65 oC for bovine primer and 60 oC for porcine primer. Therefore, hydrolysis probe methods were analyzed by annealing temperature of 60 oC for both porcine primer and bovine primer. The result showed that the hydrolysis probe was higher specificity to identify of porcine and bovine gelatin DNA than SYBR green method.

Analisis Cemaran Daging Babi pada Bakso Sapi yang Dijual di Tanjung Priok menggunakan Real-Time Polymerase Chain Reaction (RT-PCR) Zilhadia Zilhadia; Chris Adhiyanto; Ayu Gustida; Nadiah Khairunnisa
Jurnal Sains Farmasi & Klinis Vol 7, No 1 (2020): J Sains Farm Klin 7(1), April 2020
Publisher : Fakultas Farmasi Universitas Andalas

Bakso sapi sebagai makanan yang disukai oleh masyarakat Indonesia rawan terhadap cemaran daging babi karena daging sapi harganya relatif mahal. Kasus pencampuran tersebut tentu menimbulkan ketidaknyamanan bagi masyarakat muslim di Indonesia karena daging babi tidak halal dikonsumsi. Karena itu perlu dilakukan analisis cemaran daging babi pada bakso sapi. Sampel diambil dari wilayah Tanjung Priuk karena jumlah penganut yang boleh mengkonsumsi daging babi di daerah tersebut signifikan banyaknya. Bakso kontrol (100% babi, 100% sapi, campuran 50% babi) dan 10 bakso sampel di analisis menggunakan metode real-time polymerase chain reaction (RT-PCR). Isolat DNA yang digunakan adalah DNA mitokondria dengan daerah target sitokrom B. Hasil amplifikasi RT-PCR pada 3 bakso kontrol menggunakan primer babi menunjukkan bakso kontrol 100% babi dan campuran 50% babi menghasilkan kenaikan kurva amplifikasi dengan CP 22,82 dan 20,03. Sedangkan hasil amplifikasi 10 sampel bakso sapi menggunakan primer babi tidak menghasilkan kenaikan kurva amplifikasi yang menunjukkan bahwa tidak adanya DNA babi yang teramplifikasi pada produk bakso sapi tersebut.

Kejadian Luar Biasa COVID-19, Sebuah Tinjauan Literatur Secara Singkat zilhadia Zilhadia
Pharmaceutical and Biomedical Sciences Journal (PBSJ) Vol 2, No 1 (2020)
Publisher : UIN Syarif Hidayatullah Jakarta

Abstract: The world of health is totally horrendous with a disease that attacks internal breathing and can cause death. The cause of this disease was identified as Severe Acute Respiratory Syndrome Corona Virus 2 (SARS-CoV-2) and the desease called Corona Virus Desease 2019 (COVID-19). For the first time in the last two decades, a disease has an impact on the ideology, politics, economics, social, culture, psychology, defense and security of a country, and occurs globally. COVID-19 is a zoonotic disease with a mortality rate of 6,9%. Person-to-person transmission can occur through droplets or direct contact. COVID-19 will be transmitted massively if infection control is delayed or there is no adequate personal protective equipment for health workers. Confirmation of patients who are positive for COVID-19 requires a relatively long time because it requires a careful examination of RT-PCR with a limited number of devices in Indonesia. At present, there is no definitive treatment for COVID-19 even though several drugs are being investigated. To immediately identify patients and prevent further spread, doctors must be aware of travel or contact history with patients who have symptoms of COVID-19. Abstrak: Dunia tiba tiba dihebohkan dengan adanya sebuah penyakit yang menyerang pernafasan bagian dalam dan dapat menyebabkan kematian. Penyakit ini diberi nama Corona Virus Desease 2019 (COVID-19) yang disebabkan oleh Severe Acute Respiratory Syndrome Corona Virus 2 (SARS-CoV-2). Untuk pertama kalinya sepanjang 2 dekade terakhir, sebuah penyakit berdampak terhadao ideologi, politik, ekonomi, social, budaya, psikologi, pertahanan dan keamanan suatu negara, dan terjadi secara global. COVID-19 adalah penyakit zoonosis dengan tingkat kematian mencapai 6,9%. Penularan dari orang ke orang dapat terjadi melalui droplet atau kontak langsung. COVID-19 akan menular secara masif jika pengendalian infeksi terlambat atau tidak ada alat pelindung diri yang memadai bagi petugas kesehatan. Konfirmasi pasien yang positif terkena COVID-19 membutuhkan waktu yang relatif lama karena memerlukan pemeriksaan yang teliti yaitu RT-PCR dengan jumlah alat yang terbatas di Indonesia. Saat ini, tidak ada pengobatan yang pasti untuk COVID-19 meskipun beberapa obat sedang diselidiki. Untuk segera mengidentifikasi pasien dan mencegah penyebaran lebih lanjut, dokter harus mewaspadai perjalanan atau riwayat kontak dengan pasien yang mempunyai gejala COVID-19.

Mutu Sediaan Racikan Puyer di Kecamatan Ciputat Timur Ofa Suzanti Betha; Yardi Yardi; Yetika Alvionita; Zilhadia Zilhadia; Barita Juliano Siregar
Pharmaceutical and Biomedical Sciences Journal (PBSJ) Vol 1, No 1 (2019)
Publisher : UIN Syarif Hidayatullah Jakarta

Peracikan obat merupakan salah satu dari bentuk pelayanan kefarmasian yang menjadi tanggung jawab seorang apoteker di apotek dan sarana pelayanan kefarmasian lainnya. Proses peracikan yang baik akan menghasilkan sediaan yang bermutu, aman dan efektif. Sediaan obat termasuk sediaan hasil peracikan harus terjamin mutu dan keamanannya. Di Indonesia khususnya belum terdapat prosedur dan standar mutu sediaan racikan.Penelitian ini bertujuan melakukan evaluasi mutu puyer hasil racikan apotek di kecamatan Ciputat yaitu keseragaman sediaan. Sediaan puyer disampling dari semua apotek (8 apotek) yang terdapat di wilayah kecamatan Ciputat Timur. Analisa yang dilakukan adalah keragaman bobot dan keseragaman kandungan. Penetapan keragaman bobot dilakukan menggunakan timbangan analitik dan keseragaman kandungan menggunakan Spektrofotometer UV-Vis. Hasil dari penelitian ini menunjukkan bahwa, sediaan racikan puyer dari 8 apotek di kecamatan Ciputat Timur tidak memenuhi uji keragamanan bobot dan keseragaman kandungan menurut Farmakope Indonesia V.

Epidemiologi Dan Diagnosis Dengue Di Indonesia Zilhadia, Zilhadia
Majalah Ilmu Kefarmasian Vol. 4, No. 3
Publisher : UI Scholars Hub

Show Abstract | Download Original | Original Source | Check in Google Scholar

Dengue fever/DF and dengue hemorrhagic fever/DHF is a global public health problem that occured in tropical and subtropical region. Epidemic dengue occurs every years, and it continues to be a major health problem in Indonesia. Due to its asymptomatic nature, a reliable, rapid and accurate dengue diagnosis is needed. Dengue diagnosis method based on molecular dengue virus properties and it will be developed by researcher. Dengue rapid test isnewly method. This article explaine about dengue epidemiology, molecular dengue virus properties, clinical diagnosis, serology diagnosis and progress research of dengue virus.

Diferensiasi Gelatin Sapi dan Gelatin Babi pada Gummy Vitamin C Menggunakan Metode Kombinasi Spektroskopi Fourier Transform Infrared (FTIR) dan Principal Component Analysis (PCA) Zilhadia, Zilhadia; Kusumaningrum, Farida; Betha, Ofa Suzanti; Supandi, Supandi
Pharmaceutical Sciences and Research Vol. 5, No. 2
Publisher : UI Scholars Hub

Show Abstract | Download Original | Original Source | Check in Google Scholar

Gelatin is a polypeptide obtained by partial hydrolysis of collagen derived from skin and bones of bovine and porcine. It has an essential component for the manufacture of gummy vitamin C as a gel-forming agent. The porcine gelatin is haram for moslems. This study was conducted to differentiate bovine gelatin and porcine gelatin in vitamin C gummy by using Fourier Transform Infrared (FTIR) combined with PCA. Gelatin was extracted from vitamin C gummy by using acetone at -20ºC and analyzed at wave number 4000-750 cm-1. The spectrum of both bovine and porcine gelatin extract from vitamin C gummy have almost the same number absorption that can not be distinguished. Therefore, the spectrum was analyzed by PCA. PCA result, represented with the plot score, showed that both bovine and porcine gelatin extracted has a clear dictinction. PCAcombined FTIR could differentiate of them. This study obtained that the commercial vitamin C gummy contained bovine gelatin.

Co-Authors Abdi Wira Septama Afifah Nurul Izzah Ayu Gustida Barita Juliano Siregar Bayyinah Bayyinah Bayyinah, Bayyinah CHRIS ADHIYANTO CHRIS ADHIYANTO Fahrur Rahman Saputra Farida Kusumaningrum Fathmah Syafiqoh Gustida, Ayu H Herdini Khairunnisa, Nadiah Kusumaningrum, Farida Maulina Handayani Munfarikhatin, Atina Nadiah Khairunnisa Ofa Suzanti Betha Sabrina Dahlizar Salman Alfarisi Salman Alfarisi Sandra Hermanto Sanny Susanti Sofa Fajriah Supandi Supandi Supandi Supandi Supandi suryani, nelly Suzanti Betha, Ofa Syafiqoh, Fathmah Tengku Riza Zarzani N Yardi Yardi Yetika Alvionita Yuni Anggraeni

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