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All Journal HAYATI Journal of Biosciences Jurnal Rekayasa Proses MEDIA MEDIKA INDONESIANA Indonesian Journal of Cancer Chemoprevention Indonesian Journal of Biotechnology Majalah Obat Tradisional INDONESIAN JOURNAL OF PHARMACY Jurnal Rekayasa Proses
Ratna Asmah Susidarti
1Department Of Pharmaceutical Chemistry, Faculty Of Pharmacy, Universitas Gadjah Mada, Yogyakarta 55281, Indonesia. 2Cancer Chemoprevention Research Center, Faculty Of Pharmacy, Universitas Gadjah Mada, Yogyakarta 55281, Indonesia.
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Earth & Planetary Sciences Education Health Professions Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology Public Health Social Sciences

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Cytotoxic Activity of 1-(2,5-dihydroxyphenyl)-3-pyridine-2-yl-propenone on Colon Cancer Cell WiDr Nur Ismiyati; Yuli Puspito Rini; Andi Eko Wibowo; Ratna Asmah Susidarti
Indonesian Journal of Cancer Chemoprevention Vol 6, No 1 (2015)
Publisher : Indonesian Society for Cancer Chemoprevention

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14499/indonesianjcanchemoprev6iss1pp12-15

Abstract

Colon cancer is one of the most common death-caused cancer. The high mortality rate indicates that chemotherapy has not overcome cancer disease. Strategies and development of colon cancer treatment should be pursued. Compound 1-(2,5-dihydroxyphenyl)-3-pyridine-2-yl-propenone is the 2ʹ,5ʹ-dihydroxychalcone derivative, of which the B ring was substituted with 2-pyridine ring. Chalcone and its derivatives have been reported to have several biological activities, such as cytotoxic, anti-inflammatory, antiHIV, and as a tyrosine kinase inhibitor. The objectives of this research was to determine the cytotoxic activity on WiDr colon cancer cells of 1-(2,5-dihydroxyphenyl)-3-pyridine-2-yl-propenone. Cytotoxic activity was measured using MTT assay. Compound 1-(2,5-dihydroxyphenyl)-3-pyridine-2-yl-propenone inhibited WiDr cell growth with the IC50 of  16 µM. Morphology of WiDr cell showed that compound 1-(2,5-dihydroxyphenyl)-3-pyridine-2-yl-propenone inhibited cell growth in dose dependent.Keywords:  compound 1-(2,5-dihydroxyphenyl)-3-pyridine-2-yl-propenone, WiDr colon cancer cell line, cytotoxic activity
Ethyl Acetate Fraction of Caesalpinia sappan L. Enhances Cisplatin’s Cytotoxicity on HeLa Cells via G1 and S Arrest through p53 Expression Ulfatul Husnaa; Ni Putu Linda Laksmiani; Ratna Asmah Susidarti; Edy Meiyanto
Indonesian Journal of Cancer Chemoprevention Vol 8, No 2 (2017)
Publisher : Indonesian Society for Cancer Chemoprevention

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14499/indonesianjcanchemoprev8iss2pp51-60

Abstract

Cisplatin (cisp) is the first line chemotherapeutic agent for several cancer diseases which can cause significant side effects and cellular resistance. Combination-chemotherapy treatment (co-chemotherapy) was reported to be able to reduce cisp effects. Therefore, this study was carried out to investigate the cytotoxic activity of ethyl acetate fraction of C. sappan (EFC) in combination with cisp by observing apoptosis induction and cell cycle profile. Cytotoxic activity was evaluated by MTT assay. Cell cycle and apoptosis analysis were performed using flow cytometry and p53 expression was analyzed using immunocytochemistry. EFC performed cytotoxic effect on HeLa cells by showing morphological changes such as cell shrinkage, rounding and decreasing of cells viability in concentration dependent manner, giving IC50 value of 65 μg/mL. Combination of EFC and cisp in low concentration decreased cell viability into 36.86%. Further assay indicated that this combination caused redistribution of cell cycle arrest in G1 and S phases through p53 stabilization in nucleus. However, that mechanism was not followed by apoptosis. These results provide evidence to support EFC development as the enhancer of cisp effect, by improving its cytotoxicity on HeLa cells. EFC increases HeLa cells sensitivity to cisp through G1 and S cells’ arrest depending on p53 expression. Key words: co-chemotherapy, EFC, cervix cancer HeLa cells, p53, G1 and S arrest.         
Antiproliferative Effect and Apoptosis Induced in Human Cell Lines by Bruguiera gymnorhiza Barks Methanol Extract Warsinah Warsinah; Sismindari Sismindari; Ratna Asmah Susidarti
Indonesian Journal of Cancer Chemoprevention Vol 2, No 3 (2011)
Publisher : Indonesian Society for Cancer Chemoprevention

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14499/indonesianjcanchemoprev2iss3pp299-303

Abstract

The Antiproliferative effects of methanol extract from Bruguiera gymnorhiza (B. gymnorhiza) barks were tested in vitro against three human cell lines: Hela, Raji and Myeloma cells. The extract was found to have antiproliferative effects against Hela, Raji and Myeloma cells with an IC50 value of 133, 504 and 384 µg/mL, respectively. The antiproliferative test was then performed on Hela, Raji, and Myeloma cells. Cytotoxicity assay of the extract was then determined using MTT method. There were some indications of apoptosis, such DNA fragmentation, as assessed by acrydine orange- ethidium bromide staining. These results indicate that extract from B. gymnorhiza barks can induce apoptosis in human cell lines.Keywords: Antiproliferative, Apoptosis, B. gymnorhiza
Brazilein Increased Cytotoxic Activity of Doxorubicin on MCF-7/DOX Cells Ni Putu Linda Laksmiani; Ratna Asmah Susidarti; Edy Meiyanto
Indonesian Journal of Cancer Chemoprevention Vol 6, No 2 (2015)
Publisher : Indonesian Society for Cancer Chemoprevention

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14499/indonesianjcanchemoprev6iss2pp58-63

Abstract

Brazilein is a compound obtained in a large amount from the dried heartwood of Secang (Caesalpinia sappan L.). Brazilein has strong cytotoxic effect in several cancer cell lines. This research was designed to evaluate the cytotoxic effect of brazilein and its combination with a chemotherapy agent, doxorubicin on MCF-7/DOX breast cancer cells. In the cytotoxicity assay, MCF-7/DOX cells were cultured in the presence of brazilein solely and in combination with doxorubicin for 24 hours and cell viability was evaluated by using MTT assay. MTT assay showed a dose-dependent inhibition of cell proliferation with IC50 value of 37 µM. Brazilein increased doxorubicin’s cytotoxic activity on MCF-7/DOX cells. Both of single treatment with different concentration of brazilein 12.5 and 25 mM or doxorubicin 0.8 and 1 mM gave cell viability percentage above 80%, but combination of them led to decrease the cell viability percentage significantly. Based on this research, it can be concluded that brazilein is potential to be developed as a co-chemotherapy agent on breast cancer cell that have been resistant to doxorubicin. Futher study must be held to evaluate its molecular mechanism.Keywords : brazilein, doxorubicin, MCF-7/DOX, cytotoxic. 
Kinetics Study of Paracetamol Production from Para-Aminophenol and Acetic Anhydride Rifki Wahyu Kurnianto; Muhammad Fahrurrozi; Hilda Ismail; Raden Rara Endang Lukitaningsih; Indah Tri Nugraha; Pudjono Pudjono; Rochmadi Rochmadi; Ari Sudarmanto; Ratna Asmah Susidarti
Jurnal Rekayasa Proses Vol 15, No 1 (2021)
Publisher : Departemen Teknik Kimia Fakultas Teknik Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jrekpros.64551

Abstract

In the last decade, Indonesia intensifies the efforts to reduce pharmaceutical imports. One of the initiatives is establishing a paracetamol production facility to start operating in 2024. Kinetics study is needed as a basis to design the paracetamol reactor. This study investigated the optimal temperature, reactant mole ratio, and agitation speed in the reactor for paracetamol production. In this study, aqueous solution of para-aminophenol was reacted with acetic anhydride. The mole ratio of para-aminophenol to acetic anhydride was varied to 1:1, 1:1.2, 1:1.5, and 1:2 while the temperature was varied to 80 °C, 90 °C, and 110 °C. However, due to uncontrolled heat of the reaction and limitation of the mixture’s boiling point, the actual reaction temperatures were 86 °C, 90 °C, and 108 °C. In addition, the agitation speed of 250 RPM and 350 RPM were also studied. Thin layer chromatography (TLC) and densitometry were used to determine the concentration of paracetamol in the reacting mixture. The optimum temperature, reactant mole ratio, and agitation speed in this study were 108 °C, 1:1.5, and 350 RPM, respectively. In addition, a reaction performed under those operating parameters gave the reaction rate constant of 1.95 L mol-1 min-1.Keywords: acetic anhydride; kinetics; para-aminophenol; paracetamol; pharmaceutical industry A B S T R A KDalam sepuluh tahun terakhir ini, Indonesia bertekad mengurangi impor bahan baku farmasi. Salah satu upaya yang dilakukan adalah membangun fasilitas produksi parasetamol yang akan mulai beroperasi pada tahun 2024. Studi kinetika diperlukan sebagai dasar perancangan reaktor parasetamol. Oleh karena itu, penelitian ini mengkaji kondisi operasi optimal pada reaksi produksi parasetamol yang akan dibutuhkan sebagai dasar perancangan pabrik. Pada percobaan ini, para-aminofenol direaksikan dengan anhidrida asetat dengan media air. Rasio mol para-aminofenol terhadap asetat anhidrida divariasikan 1:1 1:1,2, 1:1,5, dan 1:2 sedangkan temperatur divariasikan 80 °C, 90 °C, dan 110 °C. Akan tetapi, karena panas reaksi yang tidak dikontrol dan batasan berupa titik didih dari campuran reaksi, temperatur aktual reaksi menjadi 86 °C, 90 °C, dan 108 °C. Selain itu, kecepatan putaran pengadukan juga divariasikan pada angka 250 RPM dan 350 RPM. Kromatografi lapis tipis (KLT) dan densitometri digunakan untuk menentukan konsentrasi parasetamol dalam campuran reaksi. Temperatur, rasio mol reaktan, dan kecepatan putaran pengadukan yang optimum pada penelitian ini masing-masing adalah 110 °C, 1:1,5, dan 350 RPM. Selain itu, reaksi yang dilakukan dengan kondisi operasi tersebut menghasilkan konstanta laju reaksi 1,95 L mol-1 menit-1.Kata kunci: anhidrida asetat, industri farmasi, kinetika, para-aminofenol, parasetamol
Co-Authors Abdul Manaf Ali Akhmad Darmawan Andi Eko Wibowo Ari Sudarmanto Baehaqi Bambang Tri Purwanto Edy Meiyanto Edy Meiyanto Edy Meiyanto Edy Meiyanto Edy Sulistyowati Endang Lukitaningsih Fitria Rahmi Hari Susanti Hazar B.M. Ismail Hilda Ismail, Hilda Ika Puspita Sari Indah Purwantini Indah Tri Nugraha Jenie, Riris Istighfari Julius Kulip Kurnianto, Rifki Wahyu M. Aspollah Sukari Marwadi Rahmani Maulita Cut Nuria Muhammad Fahrurrozi Mustofa Mustofa Ni Putu Linda Laksmiani Nur Ismiyati Peter G. Waterman Pudjono Pudjono Puspa Dewi Narrij Lotulung Riris Jenie Rochmadi Rochmadi Sismindari Sismindari Siti Musinah Sri Handayani Sri Handayani Sri Handayani Subagus Wahyuono Ulfatul Husnaa Wahyono . Wahyono Wahyono Warsinah Warsinah Yuli Puspito Rini Zalinar Udin