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All Journal HAYATI Journal of Biosciences AL KAUNIYAH Ovozoa: Journal of Animal Reproduction
Nova Anita
Department Of Biology, Faculty Of Mathematics And Natural Sciences, Universitas Indonesia, Depok, Indonesia
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Earth & Planetary Sciences Materials Science & Nanotechnology Veterinary

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Effect of Ethylene Glycol on Structural Integrity at Each Stage of Preantral Follicle Development Post Vitrification of Rat Ovary-Histological Analysis Nova Anita; Abinawanto Abinawanto; Ahmad Aulia Jusuf; Anom Bowolaksono; Huriyah Adani Saoemi
HAYATI Journal of Biosciences Vol. 28 No. 4 (2021): October 2021
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.28.4.304-311

Abstract

The structure of follicular tissue affects the ability to maintain the structural integrity of follicles against cryoinjury post-vitrification. Histological analysis was conducted on the structural integrity of each stage of preantral follicles post-vitrification using 7.5% and 15.0% doses of ethylene glycol (EG), and ovarian sections with HE staining were observed using an Olympus CX21 microscope connected to Optilab 3.0 lens and Image Raster software. Analysis was conducted on the ovarian cortex in the tracing line area using polygon measure tools to obtain follicle density (follicles/mm2) and follicle index (%) data. The result showed that the EG group 7.5% (KP1) increased follicle density compared to the vitrified group (KKV) in primordial (15.83±1.77) and primary (22.94±8.51) stages. Meanwhile, KP2 (EG 15%) was in primordial (41.92±6.45), primary (11.69±1.95), secondary (33.48±3.63), and tertiary (5.93±0.69) stages. KP1 increased grade 3 follicle index compared to KKV in primary (27.66±2.34), secondary (32.41±6.99), and tertiary (25.00±5.00) stages. Meanwhile, KP2 was in primary (26.87±6.68) and tertiary (25.00±5.00) stages. Both doses of 7.5% and 15.0% EG were able to maintain structural integrity at certain stages of preantral follicles. Secondary and tertiary follicles are the best stages in maintaining grade 3 follicular integrity with the addition of 7.5% EG.
The Effect of NaFeEDTA as an Iron Fortificant an Hematocrit and Blood Platelet Levels of Rats (Rattus norvegicus L.) Sprague-dawley Strain Nova Anita; Agustino Zulys; Amelia Rosvita; Aisyah Safrina
Al-Kauniyah: Jurnal Biologi Vol. 19 No. 2 (2026): AL-KAUNIYAH: JURNAL BIOLOGI
Publisher : Department of Biology, Faculty of Science and Technology, Syarif Hidayatullah State Islami

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15408/kauniyah.v19i2.46512

Abstract

The potential of NaFeEDTA as an iron fortificant can be assessed by identifying hemoglobin and red blood cell levels and their relationship to hematocrit levels. High hematocrit values can affect blood circulation. Iron can also affect platelet levels, excessive platelet aggregation can inhibit blood flow.  This study aims to identify hematocrit and blood platelet levels of rats after NaFeEDTA in soy milk (SM). The method is RAL with 25 male rats in 5 treatment groups: KK1 (without SM); KK2 (SM 10 mL/kgBW); KP1 (SM + NaFeEDTA 2.7 mg Fe/kgBW); KP2 (SM + NaFeEDTA 5.4 mg Fe/kgBW); KP3 (SM + NaFeEDTA 10.8 mg Fe/kgBW) for 14 days. Hematocrit was measured using a hematology analyzer (t0 and t14). ANOVA-LSD test (P <0.05) showed hematocrit increases in KK2, KP1, KP2, and KP3 compared to KK1, highest in KP2 (6.22%). NaFeEDTA significantly reduced platelet levels, highest decrease in KP2 (3.06% compared to KK1 and 2.81% compared to KK2). Despite the decrease, platelet levels remained normal. Thus, NaFeEDTA has the potential to fortify iron absorption at an optimal dose of 5.4 mg Fe/kgBW.
Histological structure of rat preantral follicles after vitrification using date juice as a natural extracellular cryoprotectant Nova Anita; Aisyah Safrina; Ahmad Aulia Jusuf; Rahimi Syaidah
Ovozoa: Journal of Animal Reproduction Vol. 15 No. 2 (2026): Ovozoa: Journal of Animal Reproduction
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/ovz.v15i2.2026.90-101

Abstract

Cold shock, cryoinjury, and apoptosis remain major challenges in ovarian vitrification because they compromise the structural integrity of follicular cells. Date juice (DJ), which contains natural sugars and antioxidant compounds, may provide cryoprotective effects during vitrification. This study evaluated the effectiveness of DJ combined with ethylene glycol (EG) in preserving the structural integrity of preantral follicles. Ovaries were collected from 24 female Sprague–Dawley rats and assigned to eight groups: CN and CV (0.9% NaCl), CT1 (7.5% EG), CT2 (15% EG), T1 (7.5% EG + 7.5% DJ), T2 (7.5% EG + 15% DJ), T3 (15% EG + 7.5% DJ), and T4 (15% EG + 15% DJ). After vitrification, ovarian sections were stained with hematoxylin and eosin (H&E). Follicular integrity was evaluated histologically as the percentages of intact and non-intact primordial, primary, and secondary follicles. Statistical analysis (P ≤ 0.05) showed significant differences between the non-vitrified and vitrified control groups across all follicle stages. T1 yielded the highest percentage of intact primordial follicles (95.851%) and the lowest percentage of non-intact primordial follicles (4.149%). T2 produced the best outcomes for primary and secondary follicles, with intact follicle percentages of 90.343% and 87.158%, respectively. Overall, combining EG (7.5% or 15%) with 7.5% DJ improved follicular integrity compared with EG alone. These findings suggest that DJ has potential as a natural extracellular cryoprotectant for ovarian tissue vitrification.
Co-Authors Abinawanto Agustino Zulys Ahmad Aulia Jusuf Aisyah Safrina Aisyah Safrina Amelia Rosvita ANOM BOWOLAKSONO Huriyah Adani Saoemi Rahimi Syaidah