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All Journal HAYATI Journal of Biosciences Journal of Aquatropica Asia Jurnal Ilmiah Biosaintropis (Bioscience-Tropic) Jurnal Biologi Tropis Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Jurnal TAMBORA Jurnal Warta Desa (JWD) JURNAL AGROTEKNOSAINS Seminar Ilmiah Nasional Teknologi, Sains, dan Sosial Humaniora (SINTESA)
Khotibul Umam
Study Program of Technobiology, Faculty of Technobiology, Sumbawa University of Technology, Sumbawa, Indonesia
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Computer Science & IT Earth & Planetary Sciences Economics, Econometrics & Finance Education Environmental Science Health Professions Immunology & microbiology Industrial & Manufacturing Engineering Social Sciences Other

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UJI EFEKTIFITAS FESES TERNAK (SAPI, KERBAU DAN KUDA). TERHADAP PRODUKSI BIOGAS YANG DIHASILKAN Umam, Khotibul
Jurnal TAMBORA Vol 3 No 3 (2019): EDISI 8
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (249.271 KB) | DOI: 10.36761/jt.v3i3.402

Abstract

Sumbawa, dengan jumlah populasi yang tinggi dapat menjadi salah satu alternatif dalam pemenuhan kebutuhan energi di masyarakat, dengan memanfaatkan residu (feses) ternak sebagai biogas. Tujuan dari penelitian ini untuk mengetahui jenis perlakuan yang efektif dalam menghasilkan biogas. Metode yang digunakan dalam penelitian ini disusun dengan menggunakan Rancangan Acak Lengkap (RAL) dengan empat perlakuan dan tiga ulangan. Perlakuan menggunakan sampah sayur (SS), Feses Sapi (FS), Feses Kerbau (FK), dan Feses Kuda (Fku). Fermentor berisi kotoran dan air dengan perbandingan 1:1 difermentasi selama 13 hari. Melalui penelitian ini dilakukan uji efektifitas dengan menggunakan 4 perlakuan yakni sampah sayuran (Kontrol/SS), Feses Sapi, Feses Kerbau, dan Feses Kuda. Hasil penelitian ini menujukkan bahwa feses kerbau memiliki volume gas yang lebih tinggi yaitu sebesar 75 cm3 sedangkan dari waktu fermentasi kontrol sebagai/ sampah sayuran menujukkan hasil yang lebih cepat namun, tidak menghasilkan nyala api sebagai indikator kandungan biogas yang diharapkan dari penelitian ini. Secara berturut-turut volume gas yang dihasilkan yaitu sampah sayuran 35 cm3 Feses Sapi 45 cm3 Feses Kerbau 75 cm3 dan Feses Kuda 53 cm3 hasil analisis yang dilakukan menggunakan ANOVA One Way dengan uji lanjut Least Significance Different (LSD) menunjukkan tidak adanya perbedaan signifikan dari setiap perlakuan. Selain itu dilakukan juga uji nyala api yang hasilnya menunjukkan bahwa feses sapi yang memiliki waktu nyala terlama yaitu 13 detik dibandingkan dari perlakuan lainnya. oleh karena itu, hasil penelitian ini menunjukkan bahwa penggunaan feses sapi dalam produksi biogas lebih efektif dilihat dari uji nyala.
PENGENDALIAN RESIKO BAHAYA NAIK TURUN TANGGA DENGAN MEMASANG ANTI TOE SLIP DAN PENERAPAN THREE POINT CONTACT DI AREA SRU PERTAMINA RU IV CILACAP Umam, Khotibul; Tirta, Andy
Jurnal TAMBORA Vol. 5 No. 1 (2021): EDISI 12
Publisher : Wakil Rektor 3, Direktorat Pengabdian kepada Masyarakat dan Publikasi, Universitas Teknologi Sumbawa

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36761/jt.v5i1.1000

Abstract

The occupational hazard risks that generally occur in the RFCC refinery area, namelythe event of a fall while going up or down stairs. Usually this happens because of the concept of threethe point of contact is often overlooked, namely the safe and secure use of the stairsmaintains three points of contact at all times. In addition, anti-coin innovation is also appliedslip as a double safety in working when going up and down stairs. The purpose ofThis study was to determine the effect of using anti toe slip and threepoint methodcontact in going up and down stairs and the level of understanding of workers in educationregarding the risk of hazards in the area of ??the RFCC Pertamina RU IV Cilacap Refinery. Toget comprehensive data in research, used qualitative analysis methodsdescriptive, where the data is translated from the results of field observations in the form of interviewsand a questionnaire with relevant questions. Furthermore, the data is analyzed and presentedin the form of a diagam or histogram. This study shows that the use of antitoe slip and the application of three point contact is very important because it canminimize the occurrence of falls when going up and down stairs during work.As many as 64% and 24% of respondents stated that the use of this method was very strongit is safe to work at a height because of the safety equipment that prevents slipperywhen going up the stairs. While the concept of thee point contact can help workers tocontinue to focus on positioning the hands and feet while on the stairs so that the incidentfalling can be avoided. Providing education about the use of anti toe slip and threepoint of contact is a factor that also determines the level of security at work atthe height when using the stairs. Of the 50 respondents who were observed, as much as 94%I totally agree that education makes workers more understanding and awarewith safety equipment that must be used and of course prevent fromrisk of falling while climbing stairs.
Cross-Species Amplification and Variability of Microsatellite DNA Markers in Domesticated Indonesian Mahseer; A Case Study with Tor soro, Tor douronensis and their Interspecific Hybrids Imron Imron; Agung Asrori; Khotibul Umam; Otong Zenal Arifin; Dessy Nurul Astuti
HAYATI Journal of Biosciences Vol. 29 No. 3 (2022): May 2022
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.29.3.409-416

Abstract

Indonesian mahseer (Tor spp.) are freshwater species of high economic, cultural, and conservatory values. Owing to their high values and environmental degradation, the population of Tor fish gradually decreased, and domestication efforts have been made to conserve the population. This study was aimed to assess the cross-species amplification and microsatellite genetic diversity in Indonesian mahseer Tor soro (SS), Tor douronensis (DD), and their interspecific hybrids using primers developed for Tor putitora. Eleven primer sets were used to test for amplifiability and screen genetic diversity in 40 progenies derived from those three groups. Results showed that seven primer sets (64%) successfully amplified loci. Genetic screening using the three most consistently amplifying primers showed that the number of alleles in the three populations was low, ranging from 2 to 5 alleles. The observed heterozygosity (HO) was high ranging from 0.650 to 0.789, and the fixation index (FIS) was negative, indicating heterozygosity excess. In line with other parameters, the P-values of the HW parameter of several loci-population combinations were significantly departed from equilibrium (P <0.05). A few private alleles were observed in parental line DD and the hybrids. Overall, the cross-species primers developed from T. putitora were able to amplify loci in T. soro, T. douronensis and their hybrids and genetic diversity in the hybrid population was slightly higher than those in parental lines. Possible factors driving the phenomena and practical implications of these findings on the conservation measures are discussed.
The roles of heat shock protein 70 (HSP70) and heat shock protein 90 (HSP90) in osmoregulatory and metabolic tissues of milkfish (Chanos chanos) under osmotic stress Khotibul Umam; Cheng-Hao Tang; Tsung-Han Lee
Journal of Aquatropica Asia Vol 2 No 2 (2016): Journal of Aquatropica Asia
Publisher : Jurusan Akuakultur, Universitas Bangka Belitung

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

As an euryhaline fish, milkfish was able to adapt to a wide range of environmental salinity. However, to improve the survival and health of the stressed milkfish, the roles of HSPs are needed to prevent some damages caused by salinity effects. Therefore, maintenance of osmotic homeostasis is very important for milkfish. The present study investigated the HSPs expression induced by hypotonic stress in gills, kidney (osmoregulatory organ) and liver (metabolic organ) of euryhaline milkfish. Since heat shock response is a predominant cellular stress response, two of its major components, heat shock protein 70 (HSP70) and 90 (HSP90) were examined in this study. Two major hsp genes were first identified by cloned from the transcriptome database. In addition, the protein expression were examined through seawater (SW; 35‰) and freshwater (FW) acclimation experiments (> 2 weeks). At the osmoregulatory organ (gill and kidney), the expression of gill HSP90 and 70 revealed upregulation in the FW group. While, kidney HSP90 and 70 was showed no significant different between FW and SW. On the other hand, the expression of HSP90 and 70 based on relative intensities of immunoreactive bands at liver organ was similar between the FW and SW. These findings demonstrated that compared to SW environment, the FW was more stressful to milkfish osmoregulation and effected significantly to osmoregulatory organ.
The Effectivity of Bioactive Compounds from Montanoa hibiscifolia Benth Plants in inhibiting the Growth of Candida albicans. Askar Fardiansyah; Khotibul Umam; Wawan Sujarwo; Putu Agus Hendra Wibawa
Jurnal Biologi Tropis Vol. 20 No. 3 (2020): September - Desember
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v20i3.2174

Abstract

Leucorrhoea is one of common disease in Indonesia that caused by infections of fungus Candida albicans. In dealing with this fungi, the synthetic and even an natural compound had used. Based on pre-screening data of the plants from botanical garden of Eka Karya Lipi Bali, there found apotential plant. This study aims to find the latest alternative treatment using the extract of Montanoa hiscifolia Benth plant from the collection of the "Eka Karya" LIPI Bali Botanical Garden which can inhibit fungal growth C. albicans. This research method begins with the literature skrining process using T.K Liem books and direct plant selection. After that proceed with the extraction process of 10 plant samples dose using the evaporator. The plant extracts were then tested directly on 4 types of fungi, from Eka Karya Lipi Bali’s collected.The dose used in ths study were 3%, 2%, 1% and 0.5% respectively. The final minimum dosage test was carried out using various concentrations of M. hiscifolia Benth plant extract which is the only type of best plant extract that can inhibit growth of C. albicans. Therefore, the minimum dosage test was carried out by reducing the concentration from 3 % to 0,0001 % to determine the minimum dose of extract M. hiscifolia Benth inhibiting the fungus C. albicans. This study found that at a concentration of 3 % was showed the best results for inhibition zone. On the contrary, at the concentration of 0,0001%, whereas the area of the inhibition zone was 7,2 mm and  categorized as a medium inhibitor. At the last, we were compared the dose of commercial product that oftenly used as  Leucorrhoea treatment with M. hibiscifolia benth’s bioactive and it proven the M. hibiscifolia benth  having  a hight potential in order to cope the C. albicans. Further research might as well to check it bioactive compound that specifically play important role against the C. Albican. Beside, eventually develop this plant extract into a real product in order to be used as easy as for the people who need it.
Anti Fungi Potential of Psidium cattleianum afzel ex Sabin extraction as an inhibitor of fungi Fusarium solani Lina Febriantini; Khotibul Umam; Putu Agus Hendra Wibawa; Wawan Sujarwo
Jurnal Biologi Tropis Vol. 22 No. 1 (2022): January - March
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v22i1.3167

Abstract

The use of fungicides plays an important role in controlling plant diseases caused by Fusarium. However, the development of environment friendly fungicides is considered more effective, which used the plant secondary metabolites. This study aims to find the latest alternative fungicides using Psidium Cattleianum Afzel plant extract, ex Sabine from the collection of the "Eka Karya" LIPI-Bali Botanical Garden, which can inhibit the growth of F. solani and to find the minimum dose of the plant extract in inhibiting growth of F. solani. The method used was early screening of plant and fungi, medium preparation for fusarium and minimum dose analyze. This study found that based on 19 plants tested, Psidium cattleianum proved effective in inhibiting the F. solani.  Further testing of psidium plants was carried out by looking at the minimum dose of inhibition against fusarium. The inhibition zone contained in the 1% treatment was greater than the concentrations of 0.75%, 0.50% and 0.25%. The research was continued at a concentration reduction of 0.20-0.005% with the results showing that a concentration of 0.20% to a concentration of 0.05% resulted in a moderate inhibition zone of 8 mm to 9.2 mm. Therefore, it can be concluded that the concentration of 1% is the concentration with the minimum dose of Psidium cattleianum in inhibiting the growth of the fungus Fusarium solani.
Identifikasi Keanekaragaman Tanaman Bunga sebagai Sumber Pakan Lebah Madu di Kawasan Hutan Desa Batu Dulang, Kecamatan Batu Lanteh, Sumbawa Khotibul Umam; Lili Suharli; Baso Manguntungi; Kus Dianawati; Riri R. Anggih Chaidir
Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Vol 38, No 1 (2021)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.mib.2021.38.1.1049

Abstract

Ketersedian tanaman bunga menjadi kunci penting untuk sumber pakan lebah dan madu yang dihasilkan di masyarakat Sumbawa. Penelitian ini bertujuan untuk mengidentifikasi jenis jenis tumbuhan bunga yang berpotensi menjadi sumber pakan lebah madu di kawasan hutan, Desa Batu Dulang, Sumbawa. Metode penelitian menggunakan analisis vegetasi dengan petak ukur kuadran yang berukuran 1 m x 1 m. Data tanaman yang diperoleh dianalisis dengan indeks keanaekaragaman Shanon-Wienner (H’), indeks kemerataan Shanon (E’) dan indeks dominansi Simpson (D). Hasil penelitian diperoleh data sebanyak 216 individu tanaman bunga dari 28 spesies tanaman dan terbagi dalam 12 famili. Sebanyak 23 spesies berhasil diidentifikasi, sedangkan 4 spesies belum bisa diidentifikasi. Jenis tanaman bunga terbanyak >10 individu tanaman ditemukan pada 8 spesies dengan tanaman Kirinyuh (Chromolaena odorata) sebagai spesies terbanyak dan 4 spesies tanaman dengan jumlah paling sedikit. Hasil analisis indeks keanekaragaman (H’), kemerataan (E’) dan dominansi (D) tanaman berturut turut yaitu 2.97, 0.89, dan 0.067. Nilai tersebut menunjukkan tingkat keanekaragaman tanaman yang sedang melimpah, kemerataan yang tinggi dan dominansi tanaman yang rendah. Tingginya keanaekaragaman tanaman akan memberikan dampak positif pada ketersediaan sumber pakan lebah madu yang cukup melimpah. Tentu pula harus dengan didukung oleh kondisi hutan yang terjaga kelestariannya dengan melibatkan masyarakat lokal. Penelitian selanjutnya akan difokuskan pada waktu berbunga dari tanaman sumber pakan lebah melalui kalender pembungaan sehingga diketahui tingkat kecukupan pakan bagi lebah dan kuantitas madu yang dihasilkan dalam setahun.
PEMBERDAYAAN MASYARAKAT PETERNAK MELALUI PROGRAM DEBEST (DESA BEBAS FESES) MENJADI BIOGAS DAN PUPUK ORGANIK KANDANG DI DESA SERADING Khotibul Umam
Jurnal Warta Desa (JWD) Vol. 1 No. 3 (2019): Jurnal Warta Desa (JWD)
Publisher : Lembaga Penelitian dan Pengabdian Masyarakat Universitas Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (593.638 KB) | DOI: 10.29303/jwd.v1i3.83

Abstract

Desa Serading dikenal sebagai sentra ternak Sumbawa yang memiliki potensi besar. Potensi ini karena banyaknya jumlah ternak, kelompok peternak dan sektor lain yang terlibat. Debest atau desa bebas feses merupakan inti dari program untuk mengelola feses ternak sehingga dapat dioptimalkan menjadi produk yang bermanfaat. Pelaksanaan pengabdian ini bertujuan untuk memberikan pendampingan dalam memberdayakan masyarakat peternak di desa serading untuk mengelola peternakannya secara integrative. Akan ada banyak manfaat dan sektor yang terdampak positif dengan pengelolaan berbasis bioteknologi yang diterapkan masyarakat Serading, Sumbawa. Metode pelaksanaan pemberdayaan masyarakat melalui tiga tahapan yaitu  pemetaan potensi, penyampaian potensi dan sosialisasi program pengabdian serta pelaksanaan workshop untuk penerapan secara langsung. Hasil yang diperoleh dari pelaksanaan pemberdayaan ini selanjutnya diukur melalui direct interview kepada sampling masyarakat. Melalui program pemberdayaan masyarakat peternak di Serading melalui prinsip bioteknologi, banyak teknologi dan produk yang bisa didapatkan masyarakat. Teknologi tersebut diaplikasikan dengan memanfaatkan feses ternak sebagai bahan utama untuk pembuatan biogas dan pupuk kandang organik (POK). Program pemberdayaan masyarakat Serading ini menunjukkan bahwa besarnya potensi ternak di desa serading berkorelasi positif dengan meningkatnya produk produk bioteknologi yang bisa dihasilkan dan dapat dimanfaatkan warga baik untuk penggunaan sendiri maupun dikomersialisasi.______________________
UJI EFEKTIVITAS PUPUK ORGANIK BERBASIS LIMBAH BIOGAS DAN ORGANIK KOMERSIL PADA TANAMAN PADI BANYUASIN (Oryza sativa L.) di DESA BARU TAHAN, SUMBAWA Khotibul Umam
JURNAL AGROTEKNOSAINS Vol 4, No 1 (2020): Jurnal Agroteknosains
Publisher : Universitas Quality

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36764/ja.v4i1.378

Abstract

Penelitian ini bertujuan untuk melihat pengaruh dan tingkat efektivitas dari penggunaan pupuk organic berbasis limbah biogas dan organik komersial pada pertumbuhan tanaman padi Banyuasin. Metode yg digunakn dalam penelitian ini yaitu Rancangan acak kelompok dengan beberapa perlakuan, yakni P1 = Tanpa pemberian pupuk (Kontrol), P2 = Pemberian pupuk organic limbah biogas, P3 = Pemberian pupuk organic komersial dan dianalisis menggunakan program Costat (one way anova) dan apabila menunjukkan perbedaan nyata maka akan di uji lanjut dengan uji Duncan dengan taraf nyata 5%. Data menunjukkan bahwa perbandingan uji efektivitas antara kedua jenis pupuk diperoleh pupuk organik komersial memiliki efektivitas yang baik dalam meningkatkan pertumbuhan tanaman padi banyu asin dari jumlah anakan yang lebih banyak, tinggi tanaman yang lebih baik dan berat kering tanaman yang lebih tinggi dibandingkan dengan perlakuan pupuk organic dari limbah biogas.
Antibacterial Screenings of Chromolaena odorata L. and its potential against Staphyloccocus aureus, Bacillus subtilis, Staphyloccocus typhimurium, and Escherhicia coli in Sumbawa Ainurrohmah Aulia; Khotibul Umam
Jurnal Biologi Tropis Vol. 22 No. 3 (2022): July - September
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v22i3.4220

Abstract

Kirinyuh plant extract is one of potential antibacterial sources. Metabolite extract of kirinyuh leaf extract, which contains tannins, flavonoids, saponins, and alkaloids, is known to have antibacterial activities. In order to demonstrate the effectiveness of kirinyuh leaf extract against the bacteria Staphyloccocus aureus (SA), Baciluss subtilis (BS), Staphylococus typhimurium (BT), and Escherhicia coli (EC), this study sought to identify the prosite of bioactive compounds as well as an antibacterial. The method used was leaf extraction and several doses level treatments of 0 (control), 1, 2, 4, 3, and 5 (concentrations of 20%, 40%, 60%, 80%, and 100%, respectively). This research findings showed that kirinyuh leaf extract has modest antibacterial activity against SA, BS, and ST, but that it has considerable inhibitory effect against EC. Together, this study demonstrated the potential of Kirinyuh as a antimicrobial to specific microbial since it has widely used in our society.
Co-Authors Agung Asrori Ainurrohmah Aulia Askar Fardiansyah Chaidir, Riri Rimbun Anggih Cheng-Hao Tang Dessy Nurul Astuti Imron Imron Kus Dianawati Lina Febriantini Linda Wulandari Manguntungi, Baso Otong Zenal Arifin Putu Agus Hendra Wibawa Putu Agus Hendra Wibawa Suharli, Lili Tirta, Andy Tsung-Han Lee Wawan Sujarwo Wawan Sujarwo