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All Journal HAYATI Journal of Biosciences Jurnal Ilmu Pertanian Indonesia Jurnal Teknologi Dan Industri Pangan Jurnal Ilmu Dasar Jurnal Penelitian Pascapanen Pertanian
Betty Sri Laksmi Jenie
Departemen Ilmu Teknologi Pangan, Fakultas Teknologi Pertanian, IPB, Bogor 16680, Indonesia
Agriculture, Biological Sciences & Forestry Control & Systems Engineering Earth & Planetary Sciences Industrial & Manufacturing Engineering Mathematics

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Journal : Jurnal Ilmu Dasar

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Phenotypic and genotypic identification of lactic acid bacteria isolated from spontaneous fermentation of unripe var. agung semeru banana (Musa paradisiaca formatypica) N Nurhayati; Betty Sri Laksmi Jenie; Harsi D Kusumaningrum; sri widowati
Jurnal ILMU DASAR Vol 12 No 2 (2011)
Publisher : Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Jember

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Abstract

Lactic acid bacteria (LAB) can be found on food products such as milk and meat products, cerealia, cassava, fruits or vegetable products. The objectives of this research were to identify phenotypic and genotypic of lactic acid bacteria isolated from spontaneous fermentation of unripe var agung semeru banana (Musa paradisiaca formatypica). Phenotypic identification was based on general morphology, physiological test, API and Biolog system. Genotypic identification used polymerase chain reaction (PCR) method and analyses of 16S rRNA sequence. The result showed that two groups of LAB (FSnh 1 and FSnh A isolate) can use glicerol, D-ribose, D- xylose, D-glucose, D-fructose, D-mannose, methyl α D-gluco pyranoside, N-acetyl glucosamine, esculin ferric citrate, salicin, D-celiobiose, D-saccharose, gentibiose and potassium gluconate as carbon source. Beside that FSnh 1 isolate used D-galactose, L-sorbose, L-rhamnose, and amygdalin, while FSnh A isolate used metil αD- glukopiranosa, arbutin, D-maltose, D-lactose, D-trehalose, D-turanose, and potassium 5-ketogluconate as carbon source. The genotypic identification showed that Lactobacillus sp associated with the spontaneous fermentation of var agung semeru banana were identified as Lactobacillus salivarus and Lactobacillus fructivorans.
Co-Authors . Nuratifa . Suliantari Anis Usfah Prastu Jati Bambang Pontjo Priosoeryanto Dr. Adolf J.N. Parhusip Feri Kusnandar Gatot Trimulyadi Rekso Harsi D. Kusumaningrum Herastuti Rukmini Herastuti Sri Rukmini IWAN SASKIAWAN Maggy T Suhartono Miksusanti Miksusanti Mirnawati Sudarwamto Mirnawati Sudarwanto N Nurhayati Novelina Raden Haryo Bimo Setiarto Reski Praja Putra Rifda Naufalin Rizal Syarief SEDARNAWATI YASNI Soewarno T Soekarto Sri Widowati Suliantari - Susono Saono Winiati Pudji Rahayu