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All Journal Indian Journal of Forensic Medicine & Toxicology
Yutti Amornlertwatana
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Estimation of Chronological Age from Advanced Glycation End Products Level in Vitreous Fluid Kanicnan Intui; Churdsak Jaikang; Somlada Watcharakhom; Yutti Amornlertwatana
Indian Journal of Forensic Medicine & Toxicology Vol. 15 No. 2 (2021): Indian Journal of Forensic Medicine & Toxicology
Publisher : Institute of Medico-legal Publications Pvt Ltd

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37506/ijfmt.v15i2.14301

Abstract

Background: Chronological age estimation is an important process for corpse identification. Advancedglycation end products (AGEs) are accumulated during lifetime and have been used for forensic identification.The AGEs in vitreous humor still lack of information for age estimation. Objective: To investigate correlationbetween the AGEs level contained in vitreous humor and chronological age in postmortem cases. Methods:The vitreous humor samples were collected from the postmortem cases (n=142). The AGEs levels weredetermined by spectrofluorometer and presented in microgram of quinidine hemisulfate equivalent (?g QE/mL) and nanogram of pentosidine equivalent (ng PE/mL). Stepwise linear regression was used to generatethe equation. Results: The QE and PE showed mild positive correlation (r = 0.350 and 0.195; p <0.001,respectively) while the PE/QE ratio showed moderate negative correlation with age (r = -0.561, p <0.001).The predicted equation showed error value 12 years and gave accurate prediction in range 40-59 years old.Conclusion: The AGEs contained in the vitreous humor correlated with chronological age in postmortemcases. The other fluorescence molecules might be interfere during the AGEs measurement then the specificmethods should be developed for measuring the AGEs level to decrease the error value.
Gender Differentiation in Tryptophan Metabolism in Postmortem Cases Somlada Watcharakhom; Kanicnan Intui; Yutti Amornlertwatana; Churdsak Jaikang
Indian Journal of Forensic Medicine & Toxicology Vol. 15 No. 3 (2021): Indian Journal of Forensic Medicine & Toxicology
Publisher : Institute of Medico-legal Publications Pvt Ltd

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37506/ijfmt.v15i3.15955

Abstract

Tryptophan (TRP) is an essential amino acid and is metabolized via kynurenine and serotonin pathway. Both pathways are significant role in many diseases and have been studied as biomarkers for differentiate and clarify in many diseases including forensic works. Metabolism in the body might be affected by both intrinsic and extrinsic factors. This study aimed to compare postmortem blood level of TRP and its metabolites in male and female to be applied in gender identification in forensic medicine cases. Blood samples were collected from male group (n=11) and female group (n=11) and the level of the TRP and its metabolites were identified by HPLC-DAD. The values of the TRP, metabolites and metabolite ratios did not different between the groups. While, the value of ratio of picolinic acid/ quinolinic acid different significantly (p <0.05) among the groups which might be applied for gender identification in postmortem cases. In conclusion, we found that gender have no effect on the TRP and its metabolites. By the way, the effect of gender should be expanded sample size and develop to gender identification method.
Hematological Parameters Alteration in Thai Garlic Farmers Exposed to Mixed Pesticides Chepatee Srilesin; Jirapak Ruttanapattanakul; Yutti Amornlertwatana; Rangsan Watcharakawin; Churdsak Jaikang
Indian Journal of Forensic Medicine & Toxicology Vol. 16 No. 3 (2022): Indian Journal of Forensic Medicine and Toxicology
Publisher : Institute of Medico-legal Publications Pvt Ltd

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37506/ijfmt.v16i3.18263

Abstract

Fungicides, herbicides, and insecticides are commonly agrochemicals used to eliminate pests during garliccultivation. Occupational exposure to mixed pesticides can cause hematological abnormalities. The aim of thisstudy was toassess the adverse effects of chronic mixed pesticide exposure in the garlic farmers by using thehematological parameters. The blood sample was collected from 137 Thai farmers who chronically exposed tomixed pesticide and 59 control group who were not a history of exposure to mixed pesticide. Complete BloodCount (CBC) analysis was performed to investigate the hematological parameters alteration pattern of Thaifarmers. Statistical analysis also performed by using Mann-Whitney U test. Our investigations revealed thatthemean values of white blood cell (WBC) count, lymphocyte, basophil, platelet distribution width, mean plateletvolume and platelet large cell ratio significantly decreased in the chronic exposed group but did not affect whencompared with the normal reference ranges. The results clearly demonstrated that chronic exposure to the mixedpesticides during garlic cultivation alters the hematological parameters. The farmers should be aware and checktheir health to prevent the adverse effects from pesticide intoxication.
Allele Frequencies and Forensic Statistical Parameters for 21 Autosomal Short Tandem Repeats (STRs) loci in Northern Thai Population Supakit Khacha-ananda; Yutti Amornlertwatana; Phatcharin Mahawong; Nitaya Khamkomkai
Indian Journal of Forensic Medicine & Toxicology Vol. 16 No. 3 (2022): Indian Journal of Forensic Medicine and Toxicology
Publisher : Institute of Medico-legal Publications Pvt Ltd

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37506/ijfmt.v16i3.18310

Abstract

Background: Although the genetic information of STRs has been established over the entire world, the differentcharacteristics between populations influenced the STRs information. FBI’s guideline recommended to expandadditional number of STRs loci to increase power of discrimination and exclusion. Unfortunately, the currentdata of STRs in northern Thai population have not fully covered additional loci. The aim of this study is to obtainaccurate allele frequencies of STRs among northern Thai population.Methods: The genetic profiles ofunrelated individuals were characterized by Investigator 24 plex Go kit. Allelefrequencies and forensic statistical parameters were calculated within GenoProof®3and Arlequin ver 3.5.2.2.Results: The SE33 and TH01 loci represented largest and lowest number of different alleles, respectively. Therewas no significant deviation from the Hardy–Weinberg equilibrium (HWE) after Bonferroni correction in all loci(p=0.002). The SE33 locus showed the greatest PD and PE, whereas the TPOX represented the lowest PD and PE.The studied population (northern Thai) appear to be most closely related to previously reported populationscontaining of Chinese, Japanese, and Vietnamese based on allele frequency.Conclusion: Our results recommended that current autosomal STRs data extended the application of STRs typingin parentage analyses and human identification among the localized population in northern region of Thailanddue to highly informative polymorphic data.
Co-Authors Chepatee Srilesin Churdsak Jaikang Jirapak Ruttanapattanakul Kanicnan Intui Nitaya Khamkomkai Phatcharin Mahawong Rangsan Watcharakawin Somlada Watcharakhom Supakit Khacha-Ananda