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Identifikasi Spesies Reaktif yang Dihasilkan Plasma Nonthermal lucutan penghalang dielektrik permukaan dengan Variasi Sumber Gas (Udara, N2, O2) untuk Membunuh Bakteri Renaldo Apriandi Kasa; Unggul Pundjung Juswono; Dionysius Joseph Djoko Herry Santjojo
Jurnal Penelitian Pendidikan IPA Vol. 8 No. 4 (2022): October
Publisher : Postgraduate, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jppipa.v8i4.2167

Abstract

Non-thermal plasma is one of the new techniques that is being focused on in the medical world, one of which is used for sterilization because non-thermal plasma is known to have a bactericidal effect. Reactive species produced by non-thermal plasma are antimicrobial. One factor affecting plasma species' reactive composition is the gas source used. Therefore, this study aimed to determine the effect of reactive species produced from non-thermal plasma generation of SDBD using different gas sources, namely free air, oxygen, and nitrogen, in treating Escherichia Coli bacteria. The gas flow used is 1 liter/minute. Reactive species produced by plasma were characterized using Optical Emission Spectrometry (OES). SDBD Non-thermal plasma treatment for Escherichia Coli bacteria samples was repeated three times for each treatment, and then the Anova test was performed. The results of this study indicate that bacterial death at the decontamination level depends on the composition of the gas used during treatment. Plasma using an O2 gas source has a more effective inactivation ability, namely 6 x 107 CFU/ml, compared to the control, which is 409 x 107 CFU/ml. At the same time, the treatment results with free air and nitrogen gas sources were 6.33 x 107 CFU/ml and 41.67 x 107 CFU/ml. These results indicate that the composition of ROS and RNS influences bacterial inactivation, where ROS is more effective in inactivating bacteria than RNS
bahasa inggris Margaretha Berek; Johan Andoyo Effendi Noor; Unggul Pundjung Juswono; Fatimah Kunti Hentihu; Alfian Khoiri Anto; Sigma Nur Rismawati; Ayu Hardianti Pratiwi; Karimah Karimah
Jurnal Penelitian Pendidikan IPA Vol 8 No 6 (2022): December
Publisher : Postgraduate, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jppipa.v8i6.2364

Abstract

Research has been carried out on Measuring and Determining the Output Factors of 6 and 10 MV WFF photon beams in Small Square and Rectangular fields using Semiflex TM 31010 and PintPoint TM 31014 Ionization Chamber. This study aims to determine the value of the small field output factor. Measurements were carried out in a water phantom at a depth of 10 cm with a 100 cm SSD technique. The area of ​​the irradiation field used is (2 × 2; 3 × 3; 4 × 4; 2.5 × 1.6; 3.6 × 2.5; 4.5 × 2; and 5 × 3.2) cm2. Field output factor calculations were performed using the IAEA TRS. 483 dosimetry protocol. The results of measurements and calculations obtained for the two detectors used show that a decrease in the value of the output factor occurs when the field size is getting smaller. The output factor value in a small rectangular field looks smaller when compared to the output factor value in a small square field.
The Effects of Reactive Oxygen and Nitrogen Species (RONS) Produced by Surface Dielectric Barrier Discharge (SDBD) Non-Thermal Plasma with Treatment Time and Distance Variations to Kill Escherichia coli Renaldo Apriandi Kasa; Unggul Pundjung Juswono; Dionysius J. D. H. Santjojo
Science and Technology Indonesia Vol. 8 No. 1 (2023): January
Publisher : Research Center of Inorganic Materials and Coordination Complexes, FMIPA Universitas Sriwijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26554/sti.2023.8.1.45-51

Abstract

Research on the inactivation of Escherichia coli causing diarrheal disease using non-thermal plasma SDBD has been carried out. SDBD is a new technique for non-thermal plasma generation with several advantages: low power generation, comprehensive treatment area coverage, and reducing the potential effects of burning and drying tissue. This study aimed to analyze the effect of treatment time variations, namely 0 as control, 60, 75, 90, 105, and 120 seconds and treatment distance variations of 3, 6, 9, 12, and 15 mm of non-thermal plasma treatment of SDBD on E. coli. The results of the non-thermal plasma SDBD treatment with variations in time and distance showed that the longer the treatment time, the more bacterial cells died. Colony counts decreased to 4.33 x 107 CFU/mL compared to the control, 409 x 107 CFU/mL, with a treatment time variation of 120 seconds, yielding the best treatment results. At the same time, the results of the treatment for variations in the non-thermal plasma distance of SDBD showed that the smaller the treatment distance, the greater the bacterial death rate, with the best treatment results at a 3 mm treatment interval, with colony counts of 8 x 107 CFU/mL, compared to 409 x 107 CFU/mL in control. Based on these results, SDBD non-thermal plasma treatment can be used to inactivate or kill bacteria with effectiveness in killing bacteria depending on the length of treatment time and the distance of treatment.