Nenny Prasetyaningrum
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Potential Effect of Djambal Catfish (Pangasius djambal) Gelatin as Biomaterial Product on Healing Socket after Tooth Extraction in Rats Fredy Mardiyantoro; Nenny Prasetyaningrum; Miftakhul Cahyati; Zefry Zainal Abidin; Norifumi Nakamura
Indian Journal of Forensic Medicine & Toxicology Vol. 15 No. 2 (2021): Indian Journal of Forensic Medicine & Toxicology
Publisher : Institute of Medico-legal Publications Pvt Ltd

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37506/ijfmt.v15i2.14728

Abstract

Purpose: Adequate wound healing after a tooth extraction is significant because the oral cavity has amasticatory function and plays a role in nutrient intake. Various scientific studies have been conducted tofind the ideal biomaterial to support wound healing. This study aims to determine the wound healing activityusing Djambal Catfish (Pangasius djambal) gelatin after tooth extraction.Method: Gelatin was obtained from Djambal Catfish (Pangasius djambal). The sample was selected byrandom sampling, divided into 6 groups, namely the control group on the 3rd day (n=6), the control groupon the 5th day (n=6), the control group on the 7th day (n=6), the treatment group on the 3rd day (n=6), thetreatment group on the 5th day (n=6), and the treatment on the 7th day (n=6). Wound healing was examinedby evaluating wound healing marker such as macrophages, fibroblasts, epithelialization, angiogenesis, andcollagen. Data were processed using statistical analysis.Results: The treatments had a positive result in wound healing as compared with the control groups.Histological assessment showed a remarkable sign of an increased number of macrophage, fibroblast,epithelialization, and collagen deposition.Conclusion: Gelatin derived from Djambal Catfish (Pangasius djambal) can accelerate the wound healingprocess thus could be a component therapeutic potential product that may be beneficial in wound healing inthe future.
APLIKASI TOPIKAL NANOTRANSFERSOM EKSTRAK KULIT JERUK NIPIS (Citrus aurantifolia Swingle) TERHADAP MAKROFAG PADA PROSES PENYEMBUHAN LUKA MUKOSA TIKUS WISTAR Nenny Prasetyaningrum; Ayu Fitria Adiningdyah
E-Prodenta Journal of Dentistry Vol 5 No 2 (2021)
Publisher : Fakultas Kedokteran Gigi UB

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.eprodenta.2021.005.02.4

Abstract

Latar belakang: Tanaman herbal telah banyak digunakan masyarakat sebagai obat untuk menyembuhkan luka dengan efek samping yang minimal. Salah satu tanaman yang memiliki banyak manfaat, yaitu kulit jeruk nipis (Citrus aurantifolia Swingle) yang diketahui memiliki kadar flavonoid tinggi, sehingga dapat mempercepat proses penyembuhan luka dari ulkus. Pada fase penyembuhan luka, makrofag mempunyai peran utama sebagai fagosit dan sekresi growth factor. Untuk memaksimalkan efektivitas penghantaran obat, ekstrak kulit jeruk nipis dibentuk dalam sediaan nanoTransfersom sistem. Tujuan: untuk mengetahui pengaruh pemberian nanotransfersom ekstrak kulit jeruk nipis (Citrus aurantifolia Swingle) terhadap jumlah makrofag pada penyembuhan luka mukosa labial tikus. Metode: Penelitian ini menggunakan true experimental in vivo dengan induksi termal mukosa labial tikus Wistar, terbagi 4 kelompok dengan time series hari ke-3 dan 7, yaitu kontrol negatif [K(-)], kontrol positif diberi triamcinolone acetonida 0,1% [(K(+)3], perlakuan yang diberi ekstrak kulit jeruk nipis (PA), dan diberi nanoTransfersom ekstrak kulit jeruk nipis (PB). Jumlah makrofag diamati menggunakan mikroskop perbesaran 400x. Hasil: Analisa data menggunakan uji One Way Anova menunjukkan terdapat perbedaan yang signifikan (p<0.05) pada jumlah makrofag antar kelompok pada hari ke-3 dan ke-7. Kesimpulan: NanoTransfersom ekstrak kulit jeruk nipis (Citrus aurantifolia Swingle) berpengaruh terhadap jumlah makrofag pada penyembuhan luka mukosa labial tikus wistar.
UJI EFEKTIVITAS EKSTRAK ETANOL KECAMBAH KACANG HIJAU (Vigna radiata) SEBAGAI ANTIFUNGI TERHADAP Candida albicans SECARA IN VITRO: Indonesia Lukman Hakim Hidayat; Naufal Fadhli Hardickdo; Viranda Sutanti; Nenny Prasetyaningrum
E-Prodenta Journal of Dentistry Vol 6 No 1 (2022)
Publisher : Fakultas Kedokteran Gigi UB

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.eprodenta.2022.006.01.5

Abstract

Latar Belakang: Oral candidiasis adalah infeksi oportunis rongga mulut yang disebabkan oleh Candida albicans. Namunperawatan menggunakan obat yang saat ini tersedia dapat menimbulkan efek samping. Maka dari itu diperlukan alternatif lainseperti Kecambah Kacang Hijau (Vigna radiata). Tanaman ini memiliki zat aktif berupa flavonoid dan fenol yang berfungsi sebagaipenghambat pertumbuhan Candida albicans. Tujuan: untuk membuktikan efektivitas ekstrak etanol kecambah kacang hijau(Vigna radiata L) sebagai antifungi terhadap Candida albicans secara in vitro. Metode: Penelitian ini merupakan rancanganeksperimental murni menggunakan metode dilusi cair. Penelitian diawali dengan penelitian pendahuluan menggunakan ekstrakdengan konsentrasi 12000 mg/ml, 6000 mg/ml, 3000 mg/ml, 1500 mg/ml, 750 mg/ml, dan 375 mg/ml yang kemudian dilanjutkanuji perapatan dengan konsentrasi 9000 mg/ml, 8400 mg/ml, 7800 mg/ml, 7200 mg/ml, 6600 mg/ml, 6000 mg/ml, 5400 mg/ml,dan 4800 mg/ml. Penelitian ini menggunakan kontrol negatif (konsentrasi ekstrak 0%) dan kontrol postif (nistatin 1%). Hasil:ekstrak dengan konsentrasi 9000 mg/ml dapat membunuh Candida albicans secara in vitro. Analisis data menggunakan ujiKruskal-Wallis dengan α=0,05 menunjukkan hasil signifikan dan uji korelasi Spearman menunjukkan arah korelasi negatif yangberarti semakin tinggi konsentrasi ekstrak yang digunakan, semakin sedikit koloni Candida albicans yang terbentuk. Kesimpulan:ekstrak Kecambah Kacang Hijau mampu membunuh Candida albicans dengan konsentrasi sebesar 9000 mg/ml.Kata Kunci: Candida albicans, ekstrak Kecambah Kacang Hijau, antifungi, dilusi cairABSTRACTBackground: Oral candidiasis is an opportunistic infection of the oral cavity caused by Candida albicans. However treatmentsusing currently available drugs can cause side effects. Therefore another alternative is needed such as Green Bean Sprouts (Vignaradiata). This plant has active substances in the form of flavonoids and phenols which function as inhibitors of the growth ofCandida albicans. Objective: to prove the effectiveness of ethanolic extracts of mung bean sprouts (Vigna radiata L) as anantifungal against Candida albicans in vitro. Method: This research was a purely experimental design using the liquid dilutionmethod. The study began with a preliminary study using extracts with concentrations of 12000 mg / ml, 6000 mg / ml, 3000 mg/ ml, 1500 mg / ml, 750 mg / ml, and 375 mg / ml which was then continued with a concentration test with a concentration of9000 mg / ml , 8400 mg / ml, 7800 mg / ml, 7200 mg / ml, 6600 mg / ml, 6000 mg / ml, 5400 mg / ml, and 4800 mg / ml. Thisstudy uses a negative control (extract concentration of 0%) and positive control (nystatin 1%). Result: extract with aconcentration of 9000 mg / ml can kill Candida albicans in vitro. Data analysis using the Kruskal-Wallis test with α = 0.05 showedsignificant results and the Spearman correlation test showed a negative correlation direction which meant the higher theconcentration of the extract used, the fewer colonies of Candida albicans formed. Conclusion: Green Bean Sprout extract cankill Candida albicans with a concentration of 9000 mg / ml.
PENGARUH GEL NANOTRANSFERSOME EKSTRAK KULIT JERUK NIPIS (Citrus aurantifolia Swingle) TERHADAP EKSPRESI MMP-9 PADA PENYEMBUHAN LUKA ULKUS TIKUS WISTAR Nenny Prasetyaningrum; Annisa Pramuditha Ardana
E-Prodenta Journal of Dentistry Vol 7 No 1 (2023)
Publisher : Fakultas Kedokteran Gigi UB

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.eprodenta.2023.007.01.2

Abstract

Background: Traumatic ulcers are generally caused by physical, chemical, or thermal trauma. When an ulcer occurs, wound healing processes such as hemostasis, inflammation, proliferation, and remodeling occur. In the wound healing process, the expression level of matrix metalloproteinase-9 (MMP-9) must be tightly controlled because increased expression of MMP-9 can delay wound healing by degrading the extracellular matrix (ECM) and collagen uncontrollably. Nanotransfersome gel of lime peel extract (Citrus aurantifolia Swingle) contains flavonoids, tannins, saponins, and alkaloids which have anti-inflammatory properties and can accelerate the wound healing process. Nanotransfersomes can improve tissue localization and minimize excessive drug release. Purpose: This study aims to determine the effect of lime peel extract nanotransfersome gel on the expression of MMP-9 on wound healing of wistar rats ulcers. Methods: This study used 4 groups of wistar rats with time series on days 3 and 7, namely the control group, the treatment group given lime peel extract, the treatment group given lime peel extract nanotransfersome gel, and the treatment group given standardized herbal medicine. Observation of the MMP-9 using 400x magnification. Result: Data analysis using One Way Anova test showed that there were significant differences in MMP-9 expression in the treatment groups K7 with P7B and P7A with P7B. Conclusion: Based on the results of the study it can be concluded that lime extract nanotransfersome gel can reduce MMP-9 expression in wound healing of wistar rats ulcers.   Keywords: wound healing, ulcers, nanotransfersome gel of lime peel extract, matrix metalloproteina