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Produksi Xilanase dari Isolat Sumber Air Panas Sonai, Sulawesi Tenggara, menggunakan Limbah Pertanian Susilowati, Prima Endang; Raharjo, Sapto; Kurniawati, Desi; Rahim, Rahmawati; Sumarlin, Sumarlin; Ardiansyah, Ardiansyah
Jurnal Natur Indonesia Vol 14, No 3 (2012)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (274.469 KB) | DOI: 10.31258/jnat.14.3.199-204

Abstract

Xylanase is the enzyme with prospec for hydrolysis hemicellulases. Many industry use xylanase thermostable. This processes require enzymes which are operationally stable at high temperature thus allowing e.g. easy mixing, better substrate solubility, high mass transfer rate, and lower risk of contamination. Thermophiles have often been proposed as sources of industrially relevant thermostable enzymes. Thermophilic bacteria, live at hot-springs, are can produced thermostable enzymes. In this work, we studied the bacteria strains isolated from water collected in the Sonai hot-springs of Southeast Sulawesi region and condition production enzyme. Screaning bacteria xylanolitic use semi-quantitative detection at agar plate with xilan, and quantitative detection activity enzyme. Result experinces in the 28 isolates screened, isolat IIA-3 (Pseudomonas sp.) showed the highest xylanase production. Organism efficiently used 2% rice straw as substrates. Pseudomonas sp. was used to hydrolyses rice straw at 50oC, agitation 150 rpm and pH 9.
Penentuan Jenis dan Status Konservasi Pari Layang-Layang yang Didaratkan Di TPI Gunung Lingkas Kota Tarakan Dengan Pendekatan Molekuler Syamsidar Gaffar; Sumarlin Sumarlin; M. Gandri Haryono; Harisna Pidar
Biotropika: Journal of Tropical Biology Vol 9, No 1 (2021)
Publisher : University of Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.biotropika.2021.009.01.09

Abstract

Pari yang diperdagangkan di Tempat Penampungan Ikan (TPI) Tarakan perlu diketahui jenisnya. Hal ini merupakan langkah awal agar jenis pari yang tergolong dilindungi atau populasinya telah berkurang di alam dapat dihindari untuk diperdagangkan. Penelitian ini dilakukan dengan tujuan untuk mengungkap spesies pari layang-layang yang diperdagangkan dari TPI Gunung Lingkas, Tarakan, Kalimantan Utara, dengan menggunakan metode pengidentifikasian secara molekuler (DNA barcoding) dan menentukan status konservasinya. Berdasarkan hasil BLAST, sampel teridentifikasi memiliki kemiripan 99,80% dengan spesies Aetoplatea zonura isolate GZON2. Hasil BOLD-IDS juga memberikan persentase kemiripan sebesar 99,8% dengan spesies Gymnura zonura (sinonim A. zonura). Nilai kemiripan >98% menjelaskan bahwa homologi sekuen berada pada tingkat spesies sehingga sampel yang diidentifikasi merupakan G. zonura. Hasil analisis filogenetik dengan menggunakan tiga metode analisis yaitu NJ, ML, dan ME menempatkan sampel ray-T2, G. zonura, dan G. cf zonura konsisten berada pada kelompok yang sama (monofiletik). Artinya, sampel ray-T2 berkerabat dekat dengan G. zonura. Status konservasi G. zonura terkategori vulnerable (vu) di laman IUCN yang berarti spesies ini sedang menghadapi risiko kepunahan di alam sedangkan status pada CITES masih dalam posisi belum terevaluasi.
Diversifikasi Hasil Perikanan menjadi Berbagai Olahan Pangan Bagi Kelompok PKK Kampung Enam, Kota Tarakan Kartina Kartina; Nurhikma Nurhikma; Christine Dyta Nugraeni; Tuty Alawiyah; M. Gandri Haryono; Miska Sanda Lembang; Sumarlin Sumarlin; Syamsidar Gaffar; Dena Pramita Dewi; Yane Rahayu
Literasi: Jurnal Pengabdian Masyarakat dan Inovasi Vol 1 No 2 (2021)
Publisher : Pengelola Jurnal Politeknik Negeri Ketapang

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (519.486 KB) | DOI: 10.58466/literasi.v1i2.83

Abstract

Tarakan City is one of the cities which is famous for its fishery potential. Utilization of fishery products in an efficient and integrated manner is necessary, because fishery products are very susceptible to damage or deterioration of quality so that proper handling is needed, for example through diversification into various processed products. This activity is intended to provide knowledge and skills to PKK group as motivators in the community. This activity was held on Friday, February 7, 2020 which was attended by 40 participants. Activities consist of delivering materials and demonstrations of the manufacture of processed fish and shrimp products. The result of the activity is an increase in the skills and knowledge of the participants in the diversification of various fishery products into nutritious food. Given the great benefits of this activity, it is necessary to conduct similar training to the PKK group regarding packaging techniques so that the products produced can be marketed.