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All Journal Tropical Medicine Journal Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Medula Jurnal Dunia Kesmas Jurnal Biomedika BALABA (JURNAL LITBANG PENGENDALIAN PENYAKIT BERSUMBER BINATANG BANJARNEGARA) IJES : Indonesian Journal of Environmental Sustainability.

Sitti Rahmah Umniyati

Unknown Affiliation

Author-ID : 2437798

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Earth & Planetary Sciences Energy Engineering Environmental Science Health Professions Immunology & microbiology Medicine & Pharmacology Neuroscience Nursing Public Health Veterinary

Published : 12 Documents Claim Missing Document

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Articles

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The Differences of the Prevalences and Serotypes of Dengue Virus on Aedes Aegypti Mosquitoes from Pagutan and Pagutan Timur in the Sub District of Mataram Nurul Inayati; Supargiyono -; Sitti Rahmah Umniyati
Tropical Medicine Journal Vol 2, No 1 (2012): Tropical Medicine Journal
Publisher : Pusat Kedokteran Tropis

Introduction: Sub district of Mataram is one of endemic area of Dengue Haemoraghagic Fever in the West of Nusa Tenggara province, the number of dengue cases are reported increasing every years. Pagutan is a village that has been reported as higth case incidance area and Pagutan Timur as low case insidance area.Objectives: The aims of this study is to determine the density of the Aedes aegypti mosquitoes and to know the prevalence and dominant serotypes of dengue virus in the Aedes aegypti mosquitoes from the village of Pagutan and Pagutan Timur Mataram sub district.Methods: The Collection of Aedes aegypti mosquitoes were conducted by ovitrap provided indoor and outdoor of randomly selected houses. The mosquitoes density was determined by calculating the average number of mosquitoes emerged from eggs collected each houses. The prevalence of dengue virus were calculated by the percentage of mosquitoes that containing of dengue virus as examined by immunocytochemistry method, using the monoclonal antibody anti-dengue DSSE10, while dengue virus serotypes determined by Reverse Transcriptase-Polymerase Chain Reaction using Lanciotti specific primers.Results: The density of Aedes aegypti mosquitoes from Pagutan is significantly higher than Pagutan Timur Village (p<0.05). The confirmed statistical analysis with Paired t test were obtained. The prevalence of dengue virus in Pagutan and Pagutan Timur are 18.4% and respectively 14.3%. The serotypes of dengue virus in Pagutan areas is dengue 1, dengue 2 and dengue 3, whereas in Pagutan Timur areas is dengue 1.Conclusion: There is diffences of mosquitoes density, prevalence and the serotypes of dengue virus found in Aedes aegypti mosquitoes from Pagutan and Pagutan Timur in the sub district of Mataram.Keywords: Mosquito density, Aedes aegypti, dengue virus, prevalence and serotype

Laboratory Testing of Transovarial Transmission in Aedes Aegypti Mosquito Guntur Benedict Hutadjulu; Sitti Rahmah Umniyati; Jarir At Thobari
Tropical Medicine Journal Vol 2, No 1 (2012): Tropical Medicine Journal
Publisher : Pusat Kedokteran Tropis

Introduction: Ae. aegypti is one of the vectors causing Dengue Hemorrhagic Fever (DHF) that feeds on human blood suffering from DHF and transmit it to another healthy human. The ability of Dengue Virus (DENV) to survive in nature is due to 2 mechanisms i.e. horizontal transmission among viremic vertebratae infected by Aedes mosquitos and vertical (transovarial) transmission from infected female mosquito to another generation.Objectives: To know the differences between next generation’s viral carriage, percentage ofmosquito descendants carrying DENV, and the distribution of DENV in its infected mosquitos.Methods: Study design was a quasi experimental posttest-only design with control group. The study was conducted on 53 second-generation female Ae. aegypti mosquitos which are grouped into treatment and control groups. Dengue Viral antigen in both groups were detected by immunohistochemistry method using Streptavidin Biotin Peroxidase Complex (SBPC) in paraffin embedding preparation of mosquitos aged 1-7 days. Fisher exact test was used to compare proportion of virus detected in moaquitos between 2 groups.Results: The virus detected in second generation of DEN-infected and uninfected mosquitos were 100% and 85.7%, respectively. The virus was distributed from day 1 to 7 in all organs such as brains, thorax, salivary gland, gastric epithelial cells, and ovarium of the infected mosquitos. Similar distribution was not detected in control mosquitoes.Conclusion: There was no difference in next mosquito generation’s transovarial infection among DENV-infected and uninfected Ae. aegypti mosquitos.Keywords: Ae. aegypti mosquito; Dengue virus (DENV); transovarial transmission; immunohistochemistry method, Streptavidin Biotin Peroxidase Complex (SBPC).

Head Lice Infestation and Its Relationship with Hygiene and Knowledge among Urban School Children in Yogyakarta Anastasia Joyce Lim Yit Zhen; E. Elsa Herdiana Murhandarwati; Sitti Rahmah Umniyati
Tropical Medicine Journal Vol 1, No 1 (2011): Tropical Medicine Journal
Publisher : Pusat Kedokteran Tropis

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/tmj.4565

Introduction: Pediculosis capitis is a common infestation in school children and is a worldwide public health concern. Despite this worldwide spread, incidence studies remain few. There is also a misconceptionthat head lice infestation is rarely found in the urban environment.Objectives: The objective of this study is to determine the proportion of head lice infestation among urban school children in Yogyakarta and study its relationship with hygiene and knowledge.Methods: This is a cross-sectional study conducted upon 2 schools in Yogyakarta on 154 pupils. Children range from ages 9 to 13 years. Data was collected from the schools by trained persons. A detailed questionnaire was fi lled for each pupil prior to hair examination.Results: In this research, we report the proportion of head lice infestation among urban school children in Yogyakarta and assess its risk factors. Nineteen pupils (12.3%) were infected with head lice, 5 boys(6.3%) and 14 girls (18.7%). There was a signifi cant relationship between head lice infestation and sex (P<0.05). However, no signifi cant relationship was found between head lice infestation with hygiene andknowledgeConclusion: The results show a total proportion of 12.3% among urban school children in Yogyakarta.Keywords: pediculosis capitis, urban, hygiene, knowledge, school children.

Variations in Dilution of DSSE 10 Antibody in Immunocytochemistry Technique to Detect Dengue-3 Virus in Aedes aegypti Mosquitoes Dyah Widiastuti; Sitti Rahmah Umniyati
Tropical Medicine Journal Vol 1, No 1 (2011): Tropical Medicine Journal
Publisher : Pusat Kedokteran Tropis

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/tmj.4566

Introduction: Dengue viruses, globally the most prevalent arboviruses, are transmitted to humans by persistently infected Aedes mosquitoes. The most important vector of Dengue virus is the mosquito Ae. aegypti, which should be the main target of surveillance and control activities. Virologic surveillance for Dengue viruses in its vector has been used as an early warning system to predict outbreaks. Detection of Dengue virus antigen in mosquito head squash using immunocytochemical streptavidin biotin peroxidase complex (SBPC) assay is an alternative method for Dengue vector surveillance.Objectives: The study was aimed to compare several variations of MAb DDSE10 dilutions used in immunocytochemical SBPC assay to detect Dengue virus infection in head squash of Ae. aegypti.Methods: The study design was experimental. Artifi cially-infected adult Ae. aegypti mosquitoes of DENV 3 were used as infectious samples and uninfected adult Ae. aegypti mosquitoes were used as normal ones. The immunocytochemical SBPC assay using monoclonal antibody DSSE10 with 4 variations of dilution (1:5, 1:10, 1:20, and 1:50) was applied on mosquito head squash to detect Dengue virus antigen. The results were analyzed descriptively.Results: All variants of MAb DSSE10 dilutions in immunocytochemical SBPC assay showed positive imunoreaction in infected mosquito head squash. All variants of MAb DSSE10 dilutions in immunocytochemicalSBPC assay showed negative immunoreaction in uninfected mosquito head squash.Conclusion: Monoclonal antibody DSSE10 could be used in immunochemistry technique to detect Dengue-3 virus antigen in Aedes aegypti infected intrathoracally, with 1:50 dilution.Keywords: Aedes aegypti, Dengue virus, Immunocytochemical, SPBC, Monoclonal Antibody DSSE-10

Transovarial Transmission Index of Dengue Virus on Aedes aegypti and Aedes albopictus Mosquitoes in Malalayang District in Manado, North Sulawesi, Indonesia Angle Maria Hesti Sorisi; Sitti Rahmah Umniyati; Tri Baskoro Tunggul Satoto
Tropical Medicine Journal Vol 1, No 2 (2011): Tropical Medicine Journal
Publisher : Pusat Kedokteran Tropis

Introduction: Dengue Hemorrhagic Fever (DHF) is an infectious vector-borne disease caused by Aedes sp mosquitoes still cause serious health problem in Indonesia. Based on Manado Health Office Report, Malalayang was identified as dengue-endemic areas. In 2010, number of DHF cases in Malalayang is 211 cases with Incidence Rate (IR) 328 per 100,000 populations. Dengue viruses (DENV) survive in nature by two mechanisms; by horizontal transmission through infected vertebrates and mosquitoes, and by vertical (transovarial) transmission in the mosquitoes. Transovarial transmission is assumed as an important aspect in the maintanance of DENV during inter epidemic, but this problem has not been studied in Malalayang District, Manado. An effort to prevent and control DHF requires knowledge of an Aedes sp Dengue virus transovarial infection.Objectives: To prove the existence of Dengue virus transmission in Ae. aegypti and Ae. albopictus mosquitoes and its relationship with the incidence of DHF in Malalayang District in Manado, North Sulawesi, Indonesia.Methods: The method of this research was an observational analytic study with cross-sectional design. Study samples were unbloodfed Aedes aegypti and Aedes albopictus mosquitoes on the F1 generation from ovitrap placed in five selected villages based on the number of cases in the District Malalayang. The secondary data of DHF patients from Malalayang district was obtained from Health Office Manado and the Community Health Center in 2010. The presence of dengue antigen in head squashes preparation were detected using monoclonal antibody against dengue (DSSE10) based on immunohistochemical streptavidin biotin peroxidase complex (ISBPC) technique to confirm the presence of transovarial transmission of dengue virus both in Ae. Aegypti and Ae. Albopictus, and to obtain the data of transovarial transmission index. Fisher’s Exact test and Pearson correlation are used to analyze those data.Results: Transovarial transmission of Dengue virus in Aedes sp was found from 5 villages in Malalayang district with Transovarial Transmission Index (TTI) ranges 6.1%-17.1%. Statistic test showed significant differences in positive rate (p-value=0.00<0.05) on Ae. aegypti higher than Ae. albopictus. It is also known that there is no statistically significant correlation (p-value=0.528>0.05) between the Aedes sp. Dengue virus TTI and DHF IR in Malalayang district.Conclusion: This study demonstrates the existence of Dengue virus transovarial transmission in Aedes sp in Malalayang district. Ae. aegypti’s TTI is higher than that of Ae. Albopictus, and no significant correlation between TTI and DHF IR in Malalayang district.Keywords: DHF, transovarial transmission, Ae. aegypti, Ae. albopictus

The Relationship Between The Prevalence of Head Lice Infestation with Hygiene and Knowledge Among The Rural School Children In Yogyakarta Hemelamariae Munusamy; Elisabeth Elsa Herdiana Murhandarwati; Sitti Rahmah Umniyati
Tropical Medicine Journal Vol 1, No 2 (2011): Tropical Medicine Journal
Publisher : Pusat Kedokteran Tropis

Introduction: Pediculosis capitis causes considerable social distress. Rural children are in great risk because they live in conditions where poor knowledge and hygiene are related to this matter. However, the prevalence of pediculosis capitis among school age children in rural area in Yogyakarta have not known yet.Objectives: The objective of this study is to find the prevalence of head lice infestation among the rural school children and associating it with hygiene and knowledge.Methods: This was a cross sectional study done in February 2011 in 2 primary public schools in the rural areas of Yogyakarta with 158 subjects age ranging from 8 to 16 years old. Questionnaires inclusive of hygiene and knowledge aspect were given prior to the gross visual head lice examination by trained personnel.Results: The prevalence of head lice infestation among the rural school children is 19.6%. There is no significant relationship between this with knowledge and hygiene.Conclusion: The prevalence of head lice infestation in rural school areas in Yogyakarta is 19.6%. There is no significant relationship between the prevalence of head lice infestation with hygiene and knowledge among the rural school children in Yogyakarta.Keywords: Pediculosis capitis, head lice infestation, hygiene, knowledge

Co-Authors Anastasia Joyce Lim Yit Zhen Angle Maria Hesti Sorisi Dewi Sulistyawati Dyah Widiastuti E. Elsa Herdiana Murhandarwati Guntur Benedict Hutadjulu Hari Kusnanto Hemelamariae Munusamy Jarir At Thobari Mubarak Mubarak Nastiti Wijayanti Nurminha Nurminha Nurul Inayati Nurweni, Susi Prima Widayani Supargiyono - Tri Baskoro Tunggul Satoto Wayan T. Artama

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