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Efek Imunostimulator Kubis (Brassica Oleracea Var. Capitata Alba) Terhadap Titer Imunoglobulin G (Ig G) Pada Kelinci Yang Diinduksi Dengan Sel Darah Merah Domba Nurul Inayati; Fihiruddin Fihiruddin; I Wayan Getas
Jurnal Analis Medika Biosains (JAMBS) Vol 7, No 2 (2020): JURNAL ANALIS MEDIKA BIOSAINS (JAMBS)
Publisher : Poltekkes Kemenkes Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32807/jambs.v7i2.196

Abstract

Kubis (Brassica aloracae var.capitata alba) adalah salah satu sayuran yang berpotensi sebagai imunostimulator. Kubis banyak megandung nutrien yang mempunyai nutrisi tinggi seperti vitamin, mineral, dietary fiber, glukosinolates, polyphenol dan phenolic acid. Disamping itu juga kubis mengandung peptida yang berperan sebagai imunoglobulin production stimulating factor (IPSF) sehingga dapat berfungsi sebagai imunostimulator yang akan dapat meningkatkan sistem imun tubuh dengan cara merangsang peningkatan sistem fagositik dan produksi interleukin 5. Penelitian ini merupakan penelitian ekprimental dengan desain statistic group comparation yang bertujuan mengetahui pengaruh pemberian kubis (Brassica oleracea capitata alba) untuk meningkatkan pembentukan immunoglobulin G (IgG) pada kelinci yang diinduksi dengan sel darah merah domba 2 %. Hewan coba yang digunakan dikelompokkan menjadi 2 kelompok yaitu kelompok perlakuan dan kelompok kontrol. Hewan coba yang akan digunakan adalah kelinci dengan berat 700 – 800 gr sebanyak 20 ekor yang akan dibagi menjadi dua kelompok yaitu kelompok perlakuan dan kontrol. Metode pemeriksaan yang digunakan adalah dengan uji heamaglutinasi dengan mengamati terjadinya aglutinasi antara serum yang diambil dari darah kelinci dengan sel darah merah domba   (SDMD) 2 %. Rata-rata titer Imunoglobulin G (IgG) pada kelompok perlakuan dan kelompok kontrol masing-masing adalah 2.26 µl/ml dan 1.62 µl/ml. Hasil uji statistik dengan paired t test dengan tingkat kepercayaan 0,95 % menunjukkan bahwa terdapat perbedaan yang signifikan ( p < 0,05) antara kelompok perlakuan dan kelompok kontrol yaitu dengan nilai signifikannya adalah 0,043.
ACCURATE DETECTION OF VIRAL SEROTYPE DENGUE HEMORRHAGIC FEVER THROUGH AEDES SP MOSQUITOES USING REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION (RT-PCR) Erlin Yustin Tatontos; Fihiruddin Fihiruddin; Nurul Inayati
Jurnal Riset Kesehatan Vol 10, No 2 (2021): NOVEMBER 2021
Publisher : Poltekkes Kemenkes Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (170.111 KB) | DOI: 10.31983/jrk.v10i2.7706

Abstract

Dengue Hemorrhagic Fever (DHF) caused Dengue virus which transmitted through the bite of the Aedes sp. Detection of dengue virus serotypes is essential for epidemiology as well as potential disease pathogens. RT-PCR method was more effective in mosquitoes, because the virus contained in the mosquito body is passed on to the next generation (trans ovarian). The purpose of this research is to know the serotype of DHF virus accurately through vector mosquito using RT-PCR method in Mataram City, so that vector control, early diagnosis and management of DHF disease could be done quickly and precisely. This research was a laboratory explorative research with cross sectional design that determines serotype of DHF virus through vector mosquito using RT-PCR method in Mataram City. The population of this study were Aedes aegypti female mosquitoes from eggs. Collected from houses located in Community Health Center Mataram city worked area with high dengue cases: Pagesangan, Karang Taliwang, Karang Pule, Tanjung Karang, Mataram, and Selaparang especially around the house’s sufferers. The results showed that only the mosquito group from the worked area of the Mataram Community Health Center showed the ribbon image with the base pair in accordance with serotype 1 (DENGUE 1), the other negative.
The Differences of the Prevalences and Serotypes of Dengue Virus on Aedes Aegypti Mosquitoes from Pagutan and Pagutan Timur in the Sub District of Mataram Nurul Inayati; Supargiyono -; Sitti Rahmah Umniyati
Tropical Medicine Journal Vol 2, No 1 (2012): Tropical Medicine Journal
Publisher : Pusat Kedokteran Tropis

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (5671.85 KB) | DOI: 10.22146/tmj.4290

Abstract

Introduction: Sub district of Mataram is one of endemic area of Dengue Haemoraghagic Fever in the West of Nusa Tenggara province, the number of dengue cases are reported increasing every years. Pagutan is a village that has been reported as higth case incidance area and Pagutan Timur as low case insidance area.Objectives: The aims of this study is to determine the density of the Aedes aegypti mosquitoes and to know the prevalence and dominant serotypes of dengue virus in the Aedes aegypti mosquitoes from the village of Pagutan and Pagutan Timur Mataram sub district.Methods: The Collection of Aedes aegypti mosquitoes were conducted by ovitrap provided indoor and outdoor of randomly selected houses. The mosquitoes density was determined by calculating the average number of mosquitoes emerged from eggs collected each houses. The prevalence of dengue virus were calculated by the percentage of mosquitoes that containing of dengue virus as examined by immunocytochemistry method, using the monoclonal antibody anti-dengue DSSE10, while dengue virus serotypes determined by Reverse Transcriptase-Polymerase Chain Reaction using Lanciotti specific primers.Results: The density of Aedes aegypti mosquitoes from Pagutan is significantly higher than Pagutan Timur Village (p<0.05). The confirmed statistical analysis with Paired t test were obtained. The prevalence of dengue virus in Pagutan and Pagutan Timur are 18.4% and respectively 14.3%. The serotypes of dengue virus in Pagutan areas is dengue 1, dengue 2 and dengue 3, whereas in Pagutan Timur areas is dengue 1.Conclusion: There is diffences of mosquitoes density, prevalence and the serotypes of dengue virus found in Aedes aegypti mosquitoes from Pagutan and Pagutan Timur in the sub district of Mataram.Keywords: Mosquito density, Aedes aegypti, dengue virus, prevalence and serotype
KONSENTRASI CARBOL FUCHSIN DAN WAKTU PENYIMPANAN SEDIAAN HAPUSAN SPUTUM +2 HASIL PEWARNAAN ZIEHL NEELSEN Fihiruddin Fihiruddin; Nurul Inayati
Jurnal Kesehatan Prima Vol 9, No 2 (2015): Jurnal Kesehatan Prima
Publisher : poltekkes kemenkes mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (0.272 KB) | DOI: 10.32807/jkp.v9i2.69

Abstract

Abstract: Tuberculosis (TB) is an infectious disease caused by Mycobacterium tuberculosis. Laboratory diagnosis of TB disease can be confirmed by microscopic examination using the Ziehl Neelsen Methode by carbol fuchsin dye with concentration is 0.3% which it is the standard prosedure. to reduce the error rate of performed sputum smear examination  must be carried out by laboratories established in a network of TB laboratory services. This study aims to determine the concentration of carbol fuchsin and time effective storage of performed sputum smear that show 2+ Ziehl Neelsen staining, and analyze the differences of each carbol fuchsin concentration and storage time. This study was an experimental study. The result showed the average number of acid-resistent bacteria with carbol fuchsin concentration  0.3% is as much as 23, the concentration  1% carbol fuchsin is as much as 54  and the concentration  2% carbol fuchsin is as much as 2. Based on the average amount of storage time, Acid-resistent bacteria (BTA) that storage under 1 month (0 mounth) was available as many as 34, storage for 1 month as many as 28 and stored for 2 months as many as 23, whereas stored for 3 months as many as 18. Based on the survey results know that the concentration of carbol fuchsin that provide maximum results for Ziehl Neelson staining is concentrations 1% and the storage time of performed  sputum smear  2+ is not more than 1 month. Results of statistical analysis indicating that there is significant different at each concentration carbol fuchsin and storage time with of  p value  <0.05.
Description of SGPT Levels in Construction Workers Who Have an Active Smoking Habit Eva Sapna; Nurul Inayati; Yudha Anggit Jiwantoro; Erna Kristinawati
Jurnal Analis Medika Biosains (JAMBS) Vol 11, No 1 (2024): JURNAL ANALIS MEDIKA BIOSAINS (JAMBS)
Publisher : Poltekkes Kemenkes Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32807/jambs.v11i1.349

Abstract

Physical activity is body movement that requires skeletal muscles and can cause energy expenditure. Physical activity is divided into three, namely light, moderate and heavy physical activity. Construction workers who carry out heavy physical activity for 7 to 8 hours every day can lose a lot of energy and fluids as well as important micronutrients, one of which is iron. Apart from carrying out heavy physical activities, construction workers also have an active smoking habit, which means they are considered active smokers if they smoke more than 20 cigarettes every day. Smoking is something that can cause health problems and smoking has become the biggest cause of death in the world. Cigarette raw materials such as tar, nicotine and carbon monoxide are the main toxicants that can trigger the formation of free radicals. The Research objective is to determine the description of SGPT levels in construction workers who have an active smoking habit. The methods of this research design is descriptive observational research where this research was carried out using the enzymatic kinetic method a total of 28 samples based on calculations using the Slovin formula. The results of the research is examining SGPT levels in construction workers from 28 samples showed an increase in active smokers 11-15 years, namely 3 samples and active smokers 16-20 years, namely 2 samples, there was an increase or were outside the normal value, which is where the normal value of SGPT levels in men -men, namely 42 U/L. The conclusion of this research is showed that 5 of the 28 samples had increased SGPT levels with levels of 63 U/L, 58 U/L, 52 U/L and 49 U/L in 2 samples.
Uji Efektivitas Kombinasi Ekstrak Bawang Putih (Allium sativum) dan Ekstrak Daun Srikaya (Annona squamosa L) sebagai Larvasida Aedes sp Penyebab Demam Berdarah Dengue Haryanti, Alya; Erna Kristinawati; Iswari Pauzi; Nurul Inayati
Journal of Indonesia Laboratory Students (JILTS) Vol. 4 No. 1 (2025): Journal of Indonesia Laboratory Students
Publisher : Poltekkes Kemenkes Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32807/jilts.v4i1.194

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Latar Belakang: Demam Berdarah Dengue (DBD) merupakan penyakit yang disebabkan oleh nyamuk Aedes sp. melalui gigitannya. Pengendalian DBD dalam mengatasi perkembangan vektor dilakukan dengan penggunaan larvasida sebagai insektisida yang dapat menghambat perkembangbiakan larva, tetapi penggunaan larvasida kimia memiliki efek samping yang dapat merugikan lingkungan, sehingga dibuat larvasida alternative dari bahan alami seperti bawang putih (Allium sativum) dan daun srikaya (Annona squamosa L.) keduanya memiliki kandungan yang terbukti sebagai larvasida.Tujuan Penelitian: Mengetahui efektivitas kombinasi ekstrak bawang putih dan ekstrak daun srikaya sebagai larvasida larva nyamuk Aedes sp.Metode Penelitian: Jenis penelitian ini quasi eskperiment dengan desain penelitian Post Test Only With Group Desaign. Jumlah unit percobaan 24 unit (4 perlakuan dengan 6 pengulangan) yaitu dengan konsentrasi perbandingan 40%:60%, 50%:50%, 60%:40% dan kontrol negative, serta dilakukan uji statistik One Way Anova.Hasil Penelitian: Rerata kematian larva nyamuk Aedes sp. pada konsentrasi perbandingan 40%:60% sebesar 76% kematian, konsentrasi 50%:50% sebesar 80% kematian, dan pada konsentrasi 60%:40% sebesar 88% kematian larva.Kesimpulan: Ada efektivitas kombinasi ekstrak bawang putih dan daun srikaya terhadap kematian larva Aedes sp. ditandai dengan diperoleh nilai p = 0,000 < 0,05. Kata Kunci: Larvasida, Larva Aedes sp. Bawang Putih, Daun Srikaya
Pengaruh Lama Diet Intermittent Fasting terhadap Kadar Glukosa Darah Puasa Aqila Fadia; Lale Budi Kusuma Dewi; I Wayan Getas; Nurul Inayati
Galen: Jurnal Riset Ilmu Farmasi dan Kesehatan Vol. 1 No. 2 (2025): Galen: Jurnal Riset Ilmu Farmasi dan Kesehatan
Publisher : PT Pustaka Cendekia Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.71417/galen.v1i2.62

Abstract

Intermittent fasting (IF) merupakan metode diet dengan pembatasan waktu makan, salah satunya adalah metode 16:8. Diet ini diduga berpotensi mengurangi kadar glukosa darah berkat peningkatan aktivitas insulin dan perubahan metabolisme energi. Durasi pelaksanaan diet menjadi faktor penting dalam pencapaian hasil metabolik yang optimal. Tujuannya untuk mengetahui pengaruh lama diet intermittent fasting terhadap kadar glukosa darah puasa. Penelitian ini menggunakan desain observasional analitik dengan pendekatan prospektif. Sebanyak 30 responden yang menjalani diet IF metode 16:8 diperiksa kadar glukosa darah puasanya sebelum diet, setelah 2 minggu, dan setelah 4 minggu menggunakan metode enzimatik GOD-PAP. Data dianalisis menggunakan uji Shapiro-Wilk untuk normalitas dan uji ANOVA untuk mengetahui signifikansi perbedaan. Terdapat penurunan rerata kadar glukosa darah puasa dari 119,1 mg/dl (sebelum diet) menjadi 108,63 mg/dl (setelah 2 minggu), dan 98,37 mg/dl (setelah 4 minggu). Uji ANOVA menunjukkan perbedaan yang signifikan antar ketiga waktu pengukuran (p < 0,001). Lama pelaksanaan diet intermittent fasting berpengaruh signifikan terhadap penurunan kadar glukosa darah puasa. Diet IF metode 16:8 selama 4 minggu terbukti bisa menurunkan kadar glukosa darah puasa secara bermakna dan dapat dijadikan alternatif non-farmakologis dalam pengelolaan glukosa darah.
Analisis Tingkat Infeksi Soil Transmitted Helmints (STH) dengan Kadar Haemoglobin pada Ibu Hamil di Wilayah Kerja Puskesmas Ampenan Anwariah, Zuhairatun; Nurul Inayati; Erna Kristinawati; Rohmi
Journal of Indonesia Laboratory Students (JILTS) Vol. 4 No. 1 (2025): Journal of Indonesia Laboratory Students
Publisher : Poltekkes Kemenkes Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32807/jilts.v4i1.122

Abstract

Latar Belakang: STH (Soil Transmitted Helminths) merupakan Nematoda usus membutuhkan tanah untuk proses pematangan sel telur dalam siklus hidupnya yang dapat menjadikan stadium non infektif menjadi stadium infektif. Sedangkan Hemoglobin merupakan molekul yang terdiri dari kandungan heme atau zat besi dan rantai polipeptida globin yaitu alfa, beta, gama, dan delta. Tujuan Penelitian: Mengetahui tingkat infeksi STH (Soil Transmitted Helminths) dengan kadar haemoglobin pada ibu hamil di wilayah kerja Puskesmas Ampenan. Metodelogi Penelitian: Rancangan pada penelitian ini adalah observasional analitik dengan metode cross sectional. Teknik pengambilan sampel yang digunakan adalah teknik non probability sampling yaitu accidental sampling dan uji statistik yang di gunakan adalah uji Sperman Rank, dengan jumlah sampel sebanyak 24 ibu hamil dengan Hb ≤ 11gr%. Hasil Penelitian: Berdasarkan hasil pemeriksaaan telur cacing Soil Transmitted Helmint pada ibu hamil dengan kadar Hb ≤11gr% di dapati 2 responden yang menunjukan hasil positif terinfeksi STH , sedangkan 22 responden menunjukan hasil negative dikarenakan tidak ditemukan telur cacing STH. Adapun jenis telur cacing yang ditemukan adalah Trichuris trichura. Rata-rata kadar haemoglobin ibu hamil yang di periksa feses nya adalah 10,7 gr%. Jumlah telur cacing Trichuris trichiura yang terhitung pada hasil positif dengan metode pemeriksaan Kato Katz adalah 40 per gr feses yang masuk dalam kategori tingkat infeksi rendah. Kesimpulan: Secara statistik tidak tedapat hubungan yang signifikan antara tingkat infeksi STH (Soil Transmitted Hemlinths) terhadap kejadian anemia pada ibu hamil.
PENGARUH SUPERVISI KEPALA SEKOLAH DAN BUDAYA ORGANISASI TERHADAP KINERJA GURU SD DI KECAMATAN PRINGAPUS KABUPATEN SEMARANG Nurul Inayati; Noor Miyono; Sumarno
Pendas : Jurnal Ilmiah Pendidikan Dasar Vol. 10 No. 01 (2025): Volume 10 Nomor 1, Maret 2025
Publisher : Program Studi Pendidikan Guru Sekolah Dasar FKIP Universitas Pasundan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23969/jp.v10i01.20574

Abstract

The problems in this study are (1) is there an influence of principal supervision on the performance of elementary school teachers in Pringapus District, Semarang Regency?, (2) is there an influence of organizational culture on the performance of elementary school teachers in Pringapus District, Semarang Regency?, (3) is there an influence of principal supervision and organizational culture on the performance of elementary school teachers in Pringapus District, Semarang Regency?. The objectives of this study are: (1) to determine the influence of principal supervision on the performance of elementary school teachers in Pringapus District, Semarang Regency, (2) to determine the influence of organizational culture on the performance of elementary school teachers in Pringapus District, Semarang Regency, (3) to determine the influence of principal supervision and organizational culture on the performance of elementary school teachers in Pringapus District, Semarang Regency. The population of this study was all elementary schools in Pringapus District, Semarang Regency, totaling 211 people, and with a sample of 138 people using the proportional random sampling technique. The data collection method used was the questionnaire method. The data analysis used was descriptive data analysis, prerequisite testing and hypothesis testing. Data analysis using SPSS for Window 26 program. The results of this study are as follows: (1) there is a significant influence of principal supervision on teacher performance stated in the equation Ŷ = 53.746 + 0.542X1 with a correlation strength of 0.500 with a contribution size of 0.250 = 25%, meaning that the magnitude of the influence of variable X1 on Y is 25% and the remaining 75% is influenced by other factors. The first hypothesis is proven, (2) there is a significant influence of organizational culture on performance stated in the equation Ŷ = 55.063 + 0.557X2 with a correlation strength of 0.599 with a contribution size of 0.359 = 35.9%, meaning that the influence of variable X2 on Y is 35.9% and the remaining 64.1% is influenced by other factors. The second hypothesis is proven, (3) there is a significant influence of principal supervision and organizational culture on teacher performance which is stated by the equation Ŷ=46.631+0.176X1+0.452X2 with a contribution of 0.373 or 37.3% this value shows that 37.7% of teacher performance is influenced by principal supervision and organizational culture. The remaining 62.7% is influenced by other variables. Hypothesis 3 is proven. The researcher's suggestion is for teachers to carry out learning planning professionally and systematically for the smoothness and effectiveness of the learning process, so that teacher performance will increase. For principals, they can plan, implement and evaluate supervision objectively and measurably so that teacher performance in learning planning can increase. For the education office, hold a learning innovation workshop to provide new knowledge and skills about more creative and effective teaching methods.