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Penggunaan KOH String Test Sebagai Alternatif Identifikasi Awal Bakteri Gram Negatif Elisa Rinihapsari; Shelfi Julianasya
Jurnal Ilmu Kedokteran dan Kesehatan Indonesia Vol. 1 No. 1 (2021): MARET : Jurnal Ilmu Kedokteran dan Kesehatan Indonesia
Publisher : Pusat Riset dan Inovasi Nasional

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55606/jikki.v1i1.2882

KOH string test is a simple identification method without staining, requiring only one reagent to determine the Gram reaction in bacteria. Considering the simplicity of this method, its use needs to be introduced further. However, to ensure the validity of this method, research needs to be carried out to verify the results of the KOH string test when compared with the identification method using Gram staining, which is the gold standard. This study aimed to determine whether there is a difference in the identification results between Gram staining and the KOH string test in determining the Gram characteristics of bacteria, especially Gram-negative bacteria, to find out whether there is a difference in the length of mucus produced from the KOH string-test between KOH reagent concentrations of 1, 3, and 5%, as well as knowing the KOH concentration that was best used for bacterial identification with the KOH string test. The results of the study showed that there was no difference in results between identification using Gram staining and the KOH string test. Statistical results showed a significant difference between the mucus lengths of the three reagent concentrations, and 3% KOH was the recommended reagent for use in the KOH string test method.

Pengaruh Pemanasan Berulang Media Nutrient Agar terhadap Hasil Uji ALT Bakteri Staphylococcus aureus dan Escherichia coli Elisa Rinihapsari; Benaya Yamin Onesiforus; Salsa Aten Riya
Vitamin : Jurnal ilmu Kesehatan Umum Vol. 1 No. 3 (2023): July : Jurnal ilmu Kesehatan Umum
Publisher : Asosiasi Riset Ilmu Kesehatan Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.61132/vitamin.v1i3.400

Nutrient Agar is a universal medium containing agar, meat extract, yeast extract and peptone. NA media is often made in large quantities, stored under sterile conditions, and then reheated when needed. Repeated heating can reduce the number of bacterial colonies that grow because the components that make up the media become damaged. This study aimed to determine the effect of repeated heating of NA (Nutrient Agar) media 4 times on the Total Plate Count (TPC) test results for Staphylococcus aureus and Escherichia coli bacteria. The number of bacteria that grew on the media with varying amounts of heating was calculated, and the results showed that repeated heating 4 times caused a decrease in the number of bacterial colonies that grew on the NA media. The ANOVA test gave a value of p = 0.000 for the two types of bacteria separately, which showed that there is a significant difference between the number of bacteria in varying amounts of media heating. This research concluded that repeated heating of NA (Nutrient Agar) media affects the TPC test results for Staphylococcus aureus and Escherichia coli.

Production of Poly Lactic Glycolic Acid from Solid Waste of Palm Starch Industry for Applications in the Field of Medical Biomaterials Purnavita, Sari; Rahayu, Lucia Hermawati; Rinihapsari, Elisa
World Chemical Engineering Journal VOLUME 1 NO. 5 DECEMBER 2017
Publisher : Chemical Engineering Department, Engineering Faculty, Universitas Sultan Ageng Tirtayasa

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.62870/wcej.v1i5.1241

Abstract Palm starch industry bring out solid waste that contain high total cellulose (cellulose and hemicellulose), that is 82,03%. Cellulose from solid waste of palm starch industry can be used in lactic acid production through hydrolysis and fermentation processes. Enzymatic hydrolysis and fermentation processes will work efficient and effectively when run simultaneously in one reactor, known as Simultaneous Sacharificatian and Fermentation (SSF). This research aimed to find out the most effective pH and quantity of inoculum in SSF process. This research was using substrate from solid waste of palm starch industry that treated by delignification using NaOH 7% and then saccharificated and fermented simultaneously using enzyme from Trichodermareesei for saccharification process, and Lactobacillus delbrueckii bacteria FNCC 0045 for lactic acid fermentation. The research was held by pH variation of 4, 5, 6 and 7, while quantity of inoculum were 5, 10, 15, 20 and 25% respectively. The incubation was at 46°C for 96 hour. The result showed that optimal pH was 6 and 25% inoculum was the best. Production of PLGA films was started with the polymerization reaction between lactic acid and glycolic acid at various ratios of monomer (wt%) = 95:5 and PLGA :PVA at various ratio (wt%)= 3:2, 3:3, 3:4, 3:5.The solution of PLGA in chloroform was added with PVA solution (as the film forming agents) and aloe vera gel (as an anti-microbial agents) subsequently, and then stirred with a magnetic stirrer for about 10 minutes until homogeneous. The homogeneous PLGA solution thereafter poured on the mold (glass plate). The results showed that the greater the amount of poly vinyl alcohol (ratio 3:5 % by weight), the greater the tensile strength of the film, but the lower elongation. Keywords: solid waste of palm starch industry; lactic acid; SSF;PLGA

Penggunaan KOH String Test Sebagai Alternatif Identifikasi Awal Bakteri Gram Negatif Elisa Rinihapsari; Shelfi Julianasya
Jurnal Ilmu Kedokteran dan Kesehatan Indonesia Vol. 1 No. 1 (2021): Maret : Jurnal Ilmu Kedokteran dan Kesehatan Indonesia
Publisher : Pusat Riset dan Inovasi Nasional

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55606/jikki.v1i1.2882

KOH string test is a simple identification method without staining, requiring only one reagent to determine the Gram reaction in bacteria. Considering the simplicity of this method, its use needs to be introduced further. However, to ensure the validity of this method, research needs to be carried out to verify the results of the KOH string test when compared with the identification method using Gram staining, which is the gold standard. This study aimed to determine whether there is a difference in the identification results between Gram staining and the KOH string test in determining the Gram characteristics of bacteria, especially Gram-negative bacteria, to find out whether there is a difference in the length of mucus produced from the KOH string-test between KOH reagent concentrations of 1, 3, and 5%, as well as knowing the KOH concentration that was best used for bacterial identification with the KOH string test. The results of the study showed that there was no difference in results between identification using Gram staining and the KOH string test. Statistical results showed a significant difference between the mucus lengths of the three reagent concentrations, and 3% KOH was the recommended reagent for use in the KOH string test method.

Pengaruh Pemanasan Berulang Media Nutrient Agar Terhadap Hasil Uji Sensitivitas Elisa Rinihapsari; Benaya Yamin Onesiforus; Samuel Marchel Nugroho
Antigen : Jurnal Kesehatan Masyarakat dan Ilmu Gizi Vol. 1 No. 4 (2023): November : Antigen: Jurnal Kesehatan Masyarakat dan Ilmu Gizi
Publisher : LPPM STIKES KESETIAKAWANAN SOSIAL INDONESIA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.57213/antigen.v1i3.191

Nutrient agar media is a universal medium that can be used in bacterial sensitivity tests. Sensitivity testing is used to test the sensitivity of bacteria to a type of antibiotic. This test is instrumental in determining appropriate therapy for patients who experience recurrent or persistent infectious diseases. Nutrient Agar media is usually made in large quantities for time and energy efficiency. The media is used as needed, and the rest is stored 2-8oC in the refrigerator before being used again. This research aims to determine the effect of repeated heating of Nutrient Agar media on the sensitivity test results for Staphylococcus aureus and Escherichia coli bacteria. Tests were carried out on the first, second, third and fourth heating times. The results of statistical tests with Kruskal Wallis showed a sig value of <0.05, meaning there was a difference in sensitivity test results between media heated once, twice, three times, or four times for the two types of bacteria tested.

PERBANDINGAN KEMAMPUAN FERMENTASI KHAMIR Saccharomyces cerevisiae DARI BERBAGAI MEDIA KULTUR Benaya Yamin; Elisa Rinihapsari; Firstania Tirza Diandra; Raka Pradistya
Bioma Vol. 17 No. 2 (2021): Bioma
Publisher : LPPM Universitas Negeri Jakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21009/Bioma17(2).3

Media SDA (Sabouraud Dextrose Agar) dan PDA (Potato Dextrose Agar) merupakan jenis media yang umum digunakan untuk menumbuhkan kapang dan khamir. Kekurangan dari kedua media ini adalah memiliki harga yang mahal. Sebagai alternatif, beberapa institusi pendidikan umumnya menggunakan media PDA racikan sendiri yang terbuat dari air rebusan kentang dan nutrient agar untuk menghemat biaya. Penelitian ini bertujuan untuk menelaah kualitas khamir Saccharomyces cerevisiae yang ditumbuhkan pada media SDA, PDA, dan PDA racikan. Penelitian merupakan penelitian eksperimental dengan cara menanam biakan S. cerevisiae, yang ditumbuhkan dari 3 jenis media, pada sari buah nanas. Kualitas S. cerevisiae dinilai dari kemampuan melakukan fermentasi yang dilihat dari kadar alkohol dan kadar gula reduksi. Hasil penelitian menunjukkan S. cerevisiae yang ditumbuhkan pada ketiga jenis media memiliki kualitas yang berbeda. Kadar alkohol dan kadar gula reduksi yang dihasilkan oleh S. cerevisiae yang ditumbuhkan pada media SDA (29,91% dan 0,39%), PDA (29,61% dan 0,38%), dan PDA racikan (24,02% dan 0,6%) memiliki nilai yang berbeda signifikan (p < 0,05). Kata kunci: media agar; S. cerevisiae; fermentasi; kadar alkohol; kadar gula reduksi

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