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All Journal HAYATI Journal of Biosciences Pharmaceutical Sciences and Research (PSR)
Rissyelly, Rissyelly
Departemen Farmasi, FMIPA UI
Agriculture, Biological Sciences & Forestry Earth & Planetary Sciences

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The Inhibition of 15-Lipoxygenase by Blechnum orientale Leaves and its Glycoside-flavonoid Isolates: In Vitro and In Silico Studies Rissyelly Rissyelly; Syaikhul Aziz; Frangky Sangande; Agung Wibawa Mahatva Yodha; Krisyanti Budipramana; Elfahmi Elfahmi; Sukrasno Sukrasno
HAYATI Journal of Biosciences Vol. 29 No. 3 (2022): May 2022
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.29.3.353-359

Abstract

Fern is one of the groups of primitive plants rich in secondary metabolites that are commonly used to treat various diseases, including antioxidant, anti-hyaluronidase, anti-inflammation, and respiratory disease but less investigated. Flavonoid is one of the secondary metabolites abundantly present in ferns. This study aims to isolate major compounds found in Blechnum orientale act as 15-lipoxygenase (15-LOX) inhibitors. Inhibition of lipoxygenase decrease the production of leukotriene that induces bronchoconstriction in asthma. Isoquercitrin (Quercetin-3-O-β-glucopyranoside) and trifolin (kaempferol-3-O-β-D-galactoside) have been successfully isolated from Blechnum orientale. Further in silico study was performed to explain the binding mode between flavonoid pyranoside or galactoside and flavonoid aglycone in the 15-LOX cavity and their amino acid residues interaction. Isoquercitrin binds with Ile663, Ile400, Leu408, Leu597, Ala404, and Arg403 in the 15-LOX cavity as a lipoxygenase inhibitor. Trifolin binds the same amino acids as isoquercetin with addition His366, Gln596, and Phe175. Both isoquercitrin and trifolin act as competitive inhibitors against lipoxygenase enzymes.
Uji Penghambatan Xantin Oksidase secara In Vitro Ekstrak Kulit Rambutan Putri, Nurul Eka; Rissyelly, Rissyelly; Mauldina, Marista Gilang
Pharmaceutical Sciences and Research Vol. 3, No. 1
Publisher : UI Scholars Hub

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Abstract

Hyperuricemia is a condition have higher uric acid levels that can cause a cumulation uric acid crystals in the tissues. Xanthine oxidase is an enzyme which catalyze the oxidation of hypoxanthine into xanthine and into uric acid.Therefore, the inhibition of xanthine oxidase will reduce ammount of uric acid. This research aims to determine xanthine oxidase inhibition activity and also to identify chemical constituent group of extract rambutan (Nephelium lappaceum Linn.) skin. Rambutan fruit skin was extracted by graded maceration using a three solvent, based on polarity the solvent are n-hexane, ethyl acetate and methanol. The test of inhibition xanthine oxidase activity was using a spectrophotometer at λ = 274.79 nm, pH 7.8, substrate concentration of xanthine 0.15 mM and an incubation temperature of 30°C. Inhibition on Allopurinol as positive control has IC50 0.15 μg/mL. The result showed that methanol extract of rambutan skin had the highest inhibition percentage with IC50 3.71 μg/mL. Phytochemical screening showed that the most active extract methanol of rambutan fruit skin contain flavonoids, saponins, tannins and terpenoids.
Co-Authors . Katrin Agung Wibawa Mahatva Yodha Budipramana, Krisyanti Elfahmi Elfahmi, Elfahmi Frangky Sangande Jamilah Abbas Marista Gilang Mauldina Marista Gilang Mauldina, Marista Gilang Nurul Eka Putri Nurul Eka Putri, Nurul Eka Sukrasno Sukrasno Syaikhul Aziz