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Aktivitas Antioksidan Senyawa Asam 5(E)-Eikosenoat dari Batang Tanaman Polygonum pulchrum Bl Malaka, Muhammad Hajrul; Yodha, Agung Wibawa Mahatva; Azhar, Muhammad; Hamsidi, Rini; Sahidin, Sahidin
Pharmauho: Jurnal Farmasi, Sains, dan Kesehatan Vol 1, No 2 (2015): Pharmauho
Publisher : Fakultas Farmasi Universitas Halu Oleo

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (328.093 KB) | DOI: 10.33772/pharmauho.v1i2.3462

Abstract

Isolation and identification of secondary metabolite stem bark of Polygonum pulchrum and its antioxidant activity were done. Extraction was performed by maceration using methanol solvent. The separation and purification obtained by using vacuum liquid chromatography (VLC), radial chromatography (RC) and monitored by thin layer chromatography (TLC). Identification of isolated compounds determined by spectroscopy method i.e. FT-IR, 1D-NMR (1H, 13C-NMR and DEPT) and 2-D NMR (HMQC and HMBC). White solid compound was isolated and characterized as 5(E)-eicosenoic acid. Antioxidant was analyzed towards DPPH (1,1-diphenyl-picrilhydrazil). The compound showed that has antioxidant potency with IC50 value at 348.10 µM.Keywords: Polygonum pulchrum, 5(E)-eicosenoic acid, antimicrobial, antioxidant 
The Inhibition of 15-Lipoxygenase by Blechnum orientale Leaves and its Glycoside-flavonoid Isolates: In Vitro and In Silico Studies Rissyelly Rissyelly; Syaikhul Aziz; Frangky Sangande; Agung Wibawa Mahatva Yodha; Krisyanti Budipramana; Elfahmi Elfahmi; Sukrasno Sukrasno
HAYATI Journal of Biosciences Vol. 29 No. 3 (2022): May 2022
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.29.3.353-359

Abstract

Fern is one of the groups of primitive plants rich in secondary metabolites that are commonly used to treat various diseases, including antioxidant, anti-hyaluronidase, anti-inflammation, and respiratory disease but less investigated. Flavonoid is one of the secondary metabolites abundantly present in ferns. This study aims to isolate major compounds found in Blechnum orientale act as 15-lipoxygenase (15-LOX) inhibitors. Inhibition of lipoxygenase decrease the production of leukotriene that induces bronchoconstriction in asthma. Isoquercitrin (Quercetin-3-O-β-glucopyranoside) and trifolin (kaempferol-3-O-β-D-galactoside) have been successfully isolated from Blechnum orientale. Further in silico study was performed to explain the binding mode between flavonoid pyranoside or galactoside and flavonoid aglycone in the 15-LOX cavity and their amino acid residues interaction. Isoquercitrin binds with Ile663, Ile400, Leu408, Leu597, Ala404, and Arg403 in the 15-LOX cavity as a lipoxygenase inhibitor. Trifolin binds the same amino acids as isoquercetin with addition His366, Gln596, and Phe175. Both isoquercitrin and trifolin act as competitive inhibitors against lipoxygenase enzymes.
Antioxidant and Cytotoxic Properties of Soft Coral Nepthea sp. Baru Sadarun; Nur Syifa Rahmatika; Agung Wibawa Mahatva Yodha; Adryan Fristiohady; Andini Sundowo; Syarul Nataqain Baharum; Idin Sahidin
ILMU KELAUTAN: Indonesian Journal of Marine Sciences Vol 27, No 1 (2022): Ilmu Kelautan
Publisher : Marine Science Department Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/ik.ijms.27.1.29-36

Abstract

Soft coral Nepthea sp. grows in the seas of South-East Sulawesi, Indonesia. However, information on the chemical and pharmaceutical aspects of this genus is still limited. Therefore, this research aims to explore the chemical contents and biological activities of Nepthea sp. The sample was collected from the waters of Saponda Island by SCUBA diving. It was extracted by ethyl acetate and fractionated using vacuum liquid chromatography. The chemical content was analyzed by phytochemical screening, LC-MS/MS analysis, Total Phenolics Content and Total Flavonoids Contents. Antioxidant potency was evaluated by DPPH (2,2-diphenyl-1-picrylhydrazyl) radicals and ABTS (2,2’-azino-bis-3-ethylbenzthiazoline-6-sulphonic acid). Cytotoxicity property was analyzed by MTT (3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assays. The result showed that the fractionation of Nepthea ethylacetate extracts produced six fractions (A-F). Fractions A and B contain non-polar compounds. Based on LC-MS/MS data, the non-polar compounds in Fraction A and B include achillin, atractylenolide II, buthyl isobuthyl phthalate, rengyolester, 2a-acetoxycostic acid, ocotillol acetate, petasitolone and some unidentified compounds that are C33H58O4, C15H21NO, C21H33NO, and C16H20O4. In general, the antioxidant and cytotoxic properties of all samples are in the weak category, however, when examined for each sample, the antioxidant properties of fraction B is slightly better than fraction A based on the IC50 value of DPPH and ABTS. Cytotoxicity of Fraction A is better than Fraction B against Breast Cancer cell lines MCF-7. The non-polar fraction of Nepthea sp. can be developed as raw material for the discovery of new compounds, antioxidant and anticancer agents, especially breast cancer.
Nephroprotective effect of extract Etlingera elatior (Jack) R.M. Smith on CCl4-induced nephrotoxicity in rats Adryan Fristiohady; Wahyuni Wahyuni; Muhammad Ilyas Y; Mentarry Bafadal; La Ode Muhammad Julian Purnama; Firasmi Sangadji; Muhammad Hajrul Malaka; Fadhliyah Malik; Junli Hong; Agung Wibawa Mahatva Yodha; I Sahidin
Current Research on Bioscences and Biotechnology Vol. 1 No. 2 (2020)
Publisher : Institut Teknologi Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5614/10.5614/crbb.2019.1.2/DAPM6106

Abstract

Reactive oxidative stress (ROS) can lead to cell damage, and one of them is the kidney's cell. Etlingera elatior (Jack) R.M. Smith can be utilized as an agent that can protect the cell from ROS. This study aimed to investigate the protective effect of E. elatior fruit on the kidney's cell. We used experimental animals which were treated with Na-CMC (Group I), Na-CMC (Group II), the extracts of E. elatior fruit 200, 300, 400 mg/kg BW for Group III, IV, and V, respectively, for seven days. The blood was collected after treatment. At day 8, group I, III, IV, and V were induced by CCl4. At day 9, blood was collected and the kidneys were harvested for histopathology analysis. Blood collected were measured for albumin, total protein, urea and creatinine levels. After treatment, albumin and total protein showed no increased levels; urea decreased at doses of 200, 300, and 400 mg/kg BW, respectively, and creatinine levels only decreased at the dose of 400 mg/kg BW (p<0.05). The dose of 200 and 300 mg/kg BW showed protecting effects in the tubular cells of renal. Therefore, the ethanol extract of E. elatior showed a nephroprotective effect by normalizing the urea and creatinine levels of rats and protecting tubular cells of renal.
Compounds Profile of Polar Subfraction of Ethyl Acetate Fraction of Soft Coral Nepthea sp., Antioxidant and Cytotoxic Properties Baru Sadarun; Adryan Fristiohady; Nur Syifa Rahmatika; Agung Wibawa Mahatva Yodha; Muhammad Hajrul Malaka; Andini Sundowo; I. Sahidin
Nusantara Science and Technology Proceedings 2nd Bioinformatics and Biodiversity Conference
Publisher : Future Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/nstp.2022.2103

Abstract

Nepthea sp., a soft coral In Indonesia's South-East Sulawesi, it thrives. The chemical and pharmacological properties of this genus have not been studied in this location. As a result, the goal of this page is to offer a broad overview of both properties of Nepthea sp. The sample was taken from Hoga Island. Methanol was used to extract the material, which was then fractionated with hexane and ethyl acetate to produce n-hexane, ethyl acetate, and methanol fractions. Vacuum liquid chromatography was also used to fractionate the ethyl acetate fraction. Phytochemical screening, LC-MS/MS, Total Phenolics Content (TPC), and Total Flavonoids Content (TFC) were used to determine the chemical contents. DPPH radicals and ABTS were used to assess antioxidant activity and MTT assays for cytotoxicity. Six subfractions are produced by fractioning the ethyl acetate fraction (160 g), with the most polar subfraction (F) weighing 15.4 g. The phenolics and flavonoids constituents in Subfraction F have IC50 values of 68.77±4.8 mg GAE/g extract and 0.22±0.1 mgQE/g extract, respectively. The presence of phenolics, flavonoids, and alkaloids in the subfraction F is supported by the LC-MS/MS data, Subfraction F has 3-ter-butyl-4-methoxyphenol (terpenoids or phenolics compound), isosalsoline (alkaloids), valine (amin acid), and several unidentified chemicals with molecular formulas C15H23NO3, C33H47NO4, C29H49NO3 (alkaloids or amino acid) and C8H18O3 (phenolic compound).The IC50 values (mg/L) of 101.1 ± 8.8 (DPPH) and 89.76 ± 7.5 (ABTS) indicate that F has antioxidant capacity, and it is also cytotoxic against breast cancer cell lines (MCF-7) with an IC50 of 170.72±6.5mg/mL.
Formulasi dan Karakterisasi Ekstrak Etanol Wualae (Etlingera elatior) dalam Sistem Penghantaran Vesikuler Fitofosfolipid Astrid Indalifiany; Sahidin Sahidin; Wahyuni Wahyuni; Mentarry Bafadal; Agung Wibawa Mahatva Yodha; Rina Andryani; La Ode Muhammad Fitrawan; Dian Munasari
Jurnal Mandala Pharmacon Indonesia Vol. 8 No. 1 (2022): Jurnal Mandala Pharmacon Indonesia
Publisher : Program Studi Farmasi Universitas Mandala Waluya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35311/jmpi.v8i1.152

Abstract

Etlingera elatior (Wualae) termasuk dalam tumbuhan famili zingiberaceae yang memiliki aktivitas antiinflamasi. Namun, bioavailabilitas dan permeabilitas zat aktif yang rendah sehingga diperlukan suatu sistem penghantaran dalam komplek lipid-bahan alam. Sistem yang dikenal dengan fitofosfolipid ini merupakan penghantaran fitokonstituen dalam matriks fosfolipid yang dapat meningkatkan permeabilitas dan bioavailabilitas zat aktif melalui karakteristik ampifilik dan aksi emulsifikasi oleh fosfolipid. Penelitian ini bertujuan untuk melakukan formulasi dan karakterisasi sistem vesikel sehingga diperoleh sistem yang optimum antara ekstrak etanol buah wualae dan fosfatidilkolin yang memiliki karakteristik vesikel terbaik. Preparasi dilakukan    menggunakan    teknik    hidrasi    lapis   tipis dengan adanya variasi komposisi ekstrak dan fosfatidilkolin, yakni 0,5:0,5 (Formula 1); 0,5:1 (Formula 2); 1:0,5 (Formula 3); 1:1 (Formula 4); 1:2 (Formula 5); dan 2:1 (Formula 6). Hasil yang diperoleh adalah komposisi formula optimum antara ekstrak dan fosfatidilkolin yaitu pada F4 dengan komposisi 1:1 dengan karakterisasi meliputi pengamatan morfologi berbentuk bulat (sferis) menggunakan mikroskop optic, efisiensi penjerapan vesikel diperoleh 99,20%, ukuran partikel 898,2 nm dan indeks polidispersitas 0,573 dengan kategori vesikel jenis LUV (Large Unilamellar Vesicle) menggunakan Particel Size Analyzer.
ISOLATION AND IDENTIFICATION OF ANTIOXIDANT COMPOUNDS FROM METHANOL EXTRACT OF SAPPAN WOOD (Caesalpinia sappan) Agung Wibawa Mahatva Yodha; Muamar Abdillah; Astrid Indalifiany; Elfahmi Elfahmi; Sahidin Sahidin
Jurnal Farmasi Sains dan Praktis Vol 7 No 3 (2021): Supplementary Issue (The 4th National Pharmacy Conference 2021 Universitas Halu O
Publisher : Universitas Muhammadiyah Magelang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31603/pharmacy.v7i3.6096

Abstract

Sappan wood extract (Caesalpinia sappan) provides better antioxidant properties than ascorbic acid. Empirically, Sappan wood is often used as traditional medicine. To support the utilization of Sappan wood, the isolation and identification of active antioxidant compounds from the methanol extract of Sappan wood had been conducted from this study. Extraction was performed with maceration and evaporation methods. The spots of active antioxidant compounds in TLC-DPPH observations were isolated using liquid vacuum chromatography to obtain two pure compounds. The results of the LC-MS/MS identification of the two compounds, respectively, exhibited peak fragmentation of molecular ions at m/z 271.09, 257.15, 167.03, 151.06, 123.12, and 104.06 at the retention time of 6.35 minutes which were translated as Alpinetine and peak fragmentation of molecular ion at m/z 287.09, 177.06, 164.05, 123.04 and 110.04 at a retention time of 5.75. minutes which were translated as 3-Deoxysappanone B. The antioxidant activity quantitatively showed that Alpinetine and 3-Deoxysappanone B were capable of scavenging DPPH radicals with IC50 values ​​of 20.11 M and 15.28 M, respectively.
TOKSISITAS AKUT DAN LETHAL DOSE (LD50) EKSTRAK BUAH WALAY (Meistera chinensis) ASAL SULAWESI TENGGARA TERHADAP MENCIT (Mus musculus) Musdalipah Musdalipah; Agung Wibawa Mahatya Yodha; Karmilah; Selfyana Austin Tee; Reymon; Nur Saadah Daud; Muh. Azdar Setiawan; Esti Badia; Agustini
Pharmacoscript Vol. 5 No. 2 (2022): Pharmacoscript
Publisher : Lembaga Penelitian dan Pengabdian Masyarakat, Universitas Perjuangan Tasikmalaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36423/pharmacoscript.v5i2.1039

Abstract

Buah walay (Meistera chinensis) mengandung senyawa fenolat, flavonoid, steroid, terpenoid, alkaloid, dan saponin. Beberapa metabolit sekunder, termasuk triterpenoid dan flavonoid, memiliki potensi sebagai antioksidan, antibakteri dan toksisitas. Berdasarkan penelitian sebelumnya dengan menggunakan metode Brine Shrimp Lethality Test ekstrak buah Meistera chinensis memberikan efek sangat toksik dengan IC50 dari 5,02 ± 1,11 mg/mL. Penelitian ini bertujuan untuk mengetahui efek toksik yang ditimbulkan dari ekstrak buah walay ditinjau dari penentuan nilai LD50. Jenis penelitian ini adalah penelitian eksperimental. Penentuan kategori toksisitas berdasarkan Generally Recognized As Safe/GRAS dan perhitungan LD50 menggunakan Reed-Muench Penelitian menggunakan ekor mencit yang dibagi ke dalam 5 kelompok. Kelompok kontrol diberi Na-CMC 0,5 % dan kelompok perlakuan diberi ekstrak buah dengan dosis 50 mg/KgBB; 500 mg/KgBB; 5000 mg/KgBB dan 15.000 mg/KgB Rute pemberian yang digunakan adalah secara oral. Parameter yang diamati dalam penelitian ini adalah gejala toksik yang timbul dan nilai LD50 yang diperoleh berdasarkan perhitungan jumlah hewan uji yang mati. Hasil penelitian menunjukkan bahwa ekstrak buah walay  memberikan gejala toksik berupa penurunan aktivitas gerak, laju nafas cepat serta tremor dan nilai LD50 adalah 865,765 mg/KgBB (Toksisitas ringan). Berdasarkan hasil penelitian dapat disimpulkan bahwa ekstrak buah walay (Meistera chinensis) memiliki efek toksik ringan.
Essential Oils of Alpinia monopleura and Their Antibacterial and Antioxidant Activity Agung Wibawa Mahatva Yodha; Esti Badia; Musdalipah Musdalipah; Muhammad Azdar Setiawan; Nur Saadah Daud; Angriani Fusvita; Adryan Fristiohady; Sahidin Sahidin
Molekul Vol 18 No 1 (2023)
Publisher : Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.jm.2023.18.1.6265

Abstract

Alpinia monopleura is an endemic plant which widespread and abundant in Sulawesi. Nevertheless, previous studies have not studied the chemical compound and biological activity. This study describes the chemical compounds and antibacterial and antioxidant properties of the essential oils isolated from A. monopleura. The essential oil was obtained by the hydro distillation method and interpreted by GCMS. Antibacterial properties were determined by the agar diffusion method, while DPPH and ABTS determined antioxidant activity. The most important components are α-caryophyllene, β-pinene, limonene, α-pinene, β-caryophyllene and caryophyllene oxide. Essential oils of leaves and fruit showed excellent antibacterial properties with a MIC strength of 31.3 μg/mL against Staphylococcus aureus ATCC 25023 and Escherichia coli ATCC 35218. The highest antioxidant effect was also shown by inhibiting ABTS and DPPH radicals, with IC50 strength ​​of essential oil obtained from the leaf being 15.60 and 19.42 μg/mL, respectively, while from the fruit were 10.44 and 11.93 μg/mL. These antibacterial and antioxidant properties indicate that essential oils have advantages in their use in the food and pharmaceutical industries.
Apotek Hidup: Upaya Pelestarian Penggunaan Obat Tradisional di Masyarakat melalui Pemanfaatan Tanaman Obat Keluarga Idin Sahidin; Gusti Ray Sadimantara; Muhammad Hajrul Malaka; Muhammad Azdar Setiawan; Nur Saadah Daud; Adryan Fristiohady; Yulianti Fauziah; Sernita Sernita; Musdalipah Musdalipah; Agung Wibawa Mahatva Yodha; Wahyuni Wahyuni
Jurnal Abdi dan Dedikasi kepada Masyarakat Indonesia Vol 1 No 1 (2023): NADIKAMI: Januari 2023
Publisher : POLITEKNIK BINA HUSADA KENDARI

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Penduduk Desa Lamomea Kecamatan Konda Kabupaten Konawe Selatan Provinsi Sulawesi Tenggara sebagian besar masyarakatnya berpendidikan minimal rata-rata lulusan SMA, sehingga pengetahuan tentang budidaya dan pemanfaatan tanaman obat masih sangat kurang. Salah satu upaya yang dapat dilakukan untuk membantu pelaksanaan program pemerintah terkait pemanfaatan tanaman obat. Tujuan dari kegiatan pengabdian masyarakat ini adalah untuk menyampaikan informasi tentang tanaman obat melalui sosialisasi, diskusi, dan praktik langsung budidaya tanaman obat keluarga di pekarangan rumah. Evaluasi peningkatan pengetahuan masyarakat dilakukan dengan pre-test dan post-test. Hasil dari kegiatan ini menunjukkan adanya peningkatan pengetahuan dasar tentang jenis, manfaat, dan cara membuat ramuan. Kegiatan pengabdian ini menunjukkan adanya keberhasilan dan sangat bermanfaat, karena adanya peningkatan pengetahuan dengan skor rata-rata lebih dari 60 yaitu skor rata-rata post-test 86.11 dibandingkan saat sebelum kegiatan dari nilai rata-rata pre-test 59.72. Masyarakat menunjukkan keterampilan yang memadai saat melakukan praktik langsung mulai dari penanaman, hingga pemeliharaan tanaman. Hasil budidaya tanaman obat ini, selain merupakan upaya pelestarian tanaman obat tradisional, juga mampu menghasilkan produk kosmetik yang kemudian dapat di manfaatkan sebagai produk herbal atau dijual untuk menambah nilai ekonomi.
Co-Authors Adryan Fristiohadi Adryan Fristiohady Adryan Fristiohady Adryan Fristiohady Adryan Fristiohady Adryan Fristiohady Agustini Amalia, Fajri Fitra Andini Sundowo Andini Sundowo Andini Sundowo Angriani Fusvita Arba, Muhammad Arfan Arfan Arfan Arfan Arfan Asasutjarit, Rathapon Asnani Asnani Astrid Indalifiany Austin Tee, Selfyana Azdar Setiawan, Muh. Badia, Esti Bafadal, Mentarry Baharum, Syarul Nataqain Baru Sadarun Baru Sadarun Budipramana, Krisyanti Daud, Nur Saadah Dian Munasari Elfahmi Elfahmi, Elfahmi Eny Nurhikma Esti Badia Esti Badia Fadhliyah Malik Fauziah, Yulianti Firasmi Sangadji Firdayanti, Firdayanti Fitrawan, La Ode Muhammad Frangky Sangande Fristiohady, Adryan Fusvita, Angriani Gusti Ayu Kade Sutariati Gusti Ray Sadimantara Harnita, Wa Ode Haruna, Lidya Agriningsih Haslianti Haslianti, Haslianti I Sahidin I Wayan Sutapa I. Sahidin Idin Sahidin Idin Sahidin Idin Sahidin Idin, Sahidin Ilyas Y, Muhammad Imran Irnawati Irnawati Irvan Anwar Jumadil Junli Hong Karmilah Karmilah Karmilah Karmilah Karmilah, Karmilah La Ode Muh. Fitrawan La Ode Muhammad Julian Purnama Lisa Wulansari Siregar Loly Subhiaty Idrus Malaka, Muh. Hajrul Malaka, Muhammad Hajrul Malaka, Muhammad Hajrul Muamar Abdillah Muh. Arnas Muh. Azdar Setiawan Muhammad Azdar Setiawan Muhammad Azhar Muhammad Hajrul Malaka Muhammad Hajrul Malaka Muhammad Hajrul Malaka Muhammad Ilyas Y Muhammad Israwan Azis Murtafia Murtafia Musdalipah Musdalipah Musdalipah Musdalipah Musdalipah Musdalipah Musdalipah, Musdalipah Nirwati Rusli Nohong Nur Saadah Daud Nur Saadah Daud Nur Syifa Rahmatika Nur Syifa Rahmatika Nurcahyani, Fitria Nurpajriani, Nurpajriani Nurramadhani A. Sida Nursaadah Daud Pascayantri, Asniar Purnama, La Ode Muh. Julian Purnama, La Ode Muhammad Julian Rahmat Muliadi Randa Wulaisfan Reymon Reymon Rina Andryani Rini Hamsidi Rissyelly Rissyelly Sahidin Sahidin . Sahidin Sahidin Sahidin Sahidin Sahidin Sahidin Sahidin Sahidin Sahidin, Idin Santi Oktaviani, Santi Saripuddin Selfyana Austin Tee Sernita Sernita Setiawan, Muh. Azdar Sitti Raodah Nurul Jannah Solo, Dian Munasari Sukrasno Sukrasno Syaikhul Aziz Syamsu Alam Syarul Nataqain Baharum Tee, Selfyana Austin THAMRIN AZIS WAHYUNI Wahyuni Wahyuni Wahyuni Wahyuni wahyuni wahyuni Yulianti Fauziah