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The detection of infectious bronchitis viral antigen by means of immunohistochemical technique in broiler chicken infected with I-269 IB isolate or injected with H-120 live vaccine Rini Damayanti; Darminto .
Jurnal Ilmu Ternak dan Veteriner Vol 6, No 4 (2001): DECEMBER 2001
Publisher : Indonesian Center for Animal Research and Development (ICARD)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (164.179 KB) | DOI: 10.14334/jitv.v6i4.247

Abstract

A study was carried out to detect the antigen of infectious bronchitis vius (IBV) in broiler chicken by means of immunohistochemical technique. A total of 150 - fourteen days old broiler chicken were divided into three groups i.e. 50 chicken were infected with an IB isolate of I-269, 50 chicken were injected with H-120 life vaccine, and 50 chicken served as un-treated control. Clinical signs and gross pathological changes were observed. Each of five chicken of each group were necropsied at 1, 2, 3, 4, 7, 10, 14, 21, 28, and 35 day(s) post infection/vaccination. The antigen could be detected at one day through 35 days post vaccination/infection. In the vaccinated group, histopathological lesions and the detected antigen were minimal. In contrast, the infected chicken showed varied histolopathological lesion in accordance with the numerous antigens. The antigen were observed in the lymphocytes/macrophages in the trachea, lungs and kidney, and in the epithelium of trachea, alveoli, broncheolus and tubular sitoplasm of the kidney of both vaccinated and infected groups. In the infected group, antigen was also detected in the lymphocytes and macrophages of the affected organs.   Key words: Infectious bronchitis, broiler chicken, I-269 IB isolate, immunohistochemistry
Application of Polymerase Chainreaction (PCR) In Diagnosis of Malignant Catarrhal Fever (MCF) in Indonesia Muharam Saepulloh; Darminto .
WARTAZOA, Indonesian Bulletin of Animal and Veterinary Sciences Vol 8, No 2 (1999)
Publisher : Indonesian Center for Animal Research and Development

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (832.395 KB) | DOI: 10.14334/wartazoa.v8i2.733

Abstract

Malignant catarrhal fever (MCF) is a fatal viral disease of cattle, buffalo and other ruminants characterized by proliferation and infiltration of lymphocytes and followed by necroses of infected organs . According to the aetiological of the agents, MCFis classified into two types: wildebeest-associatedMCF(WA-MCF) which is caused by Alcelaphinae herpesvirus-1 (AHV-1) and sheep-associated MCF (SA-MCF) which is caused by Ovine herpesvirus-2 (OHV-2). Most MCF cases in Indonesia is SA-MCF. Due to the intact virus of SA-MCF has not been able isolated, the diagnosis of this disease is based on the clinical signs and pathological changes. However, a segment of DNA virus of SA-MCF has been isolated from MCF cases in rabbit, deer, and cattle which provided an opportunity to study a DNA sequencing and led to the development of diagnostic technique based on the molecular biology, Polymerase Chain Reaction (PCR). The PCR technique was able to detect segments of DNA of SA-MCF virus in infected organs . This publication describes the application of PCR for diagnosis of SA-MCF in  Indonesia. Key words : MCF, PCR, diagnosis, cattle, buffalo
Epidemiology, Diagnosis and Control of Infectious Laryngotracheitis in Chickens Muharam Saepulloh; Darminto .
WARTAZOA, Indonesian Bulletin of Animal and Veterinary Sciences Vol 8, No 1 (1999)
Publisher : Indonesian Center for Animal Research and Development

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (773.943 KB) | DOI: 10.14334/wartazoa.v8i1.738

Abstract

Infectious laryngotracheitis (ILT) is an acute, highly contagious respiratory disease of poultry characterized by respiratory disorder such as coughing with blood exudate from the trachea. The disease is caused by Herpesvirus of the family Herpesviridae and subfamily of Alphaherpesvirinae. The virus has been characterized as Gallid herpesvirus-1. ILT is worldwide distribution and has been reported to be present in Indonesia. However, the information on the disease in this country is limited. Spread of the ILT among chickens can be by inhalation or digestion, but ELT virus is not transmitted vertically by eggs . The morbidity rate of the disease is about 90-100% with mortality rate between 10-70%. ILT may reduce body weight gain and reduce egg production, so it causes lost in layers, broilers as well as breeders . Diagnosis of the disease can be based on the isolation and identification of the virus using embryonated chicken eggs. There is no treatment available for ILT, so the control of the disease is mainly by vaccination. To ensure the results of vaccination program, monitoring antibody titres following vaccination is essentially required. The most widely used serological test for antibody monitoring is enzyme-linked immunosorbent assay (ELISA). Keywords : ILT, epidemiology, diagnosis, control, chicken
Cases of Anthrax in Humans and Animal in Bogor at the Beginning 2001 Susan M Noor; Darminto .; S Hardjoutomo
WARTAZOA, Indonesian Bulletin of Animal and Veterinary Sciences Vol 11, No 2 (2001)
Publisher : Indonesian Center for Animal Research and Development

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (221.569 KB) | DOI: 10.14334/wartazoa.v11i2.752

Abstract

Anthrax is a zoonotic disease caused by Bacillus anthracis. The incidence of anthrax in human and animal has been reported in Indonesia including the case of anthrax in ostrich in Purwakarta in 1999. Virtually all animals, to some degree are susceptible to anthrax but ruminants are considered to be the most susceptible. There are 11 provinces in Indonesia reported as endemic areas. Retrospective study of anthrax in Bogor reported that the disease has been spread in nine areas. The manifestation of human anthrax could be visceral, cutaneus, pulmonum, meningitis or combination but the cutaneus type is the most frequent (95%). In early 2001, there was an outbreak of anthrax affecting both human and animals in Bogor. A definitive diagnosis of anthrax has been made in 5 people in Hambalang village, Citeureup subdistrict of Bogor. The objective of this study is to describe the chronological event of the recent anthrax wabah in Bogor and to give a brief discussion of anthrax in human and animal.   Key word: Antraks cases, human beings, animals, Bogor, 2001
Study and Control of Newcastle Disease in Ducks Muharam Saepulloh; Darminto .
WARTAZOA, Indonesian Bulletin of Animal and Veterinary Sciences Vol 15, No 2 (2005): JUNE 2005
Publisher : Indonesian Center for Animal Research and Development

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (910.191 KB) | DOI: 10.14334/wartazoa.v15i2.830

Abstract

A study on the infection of Newcastle disease (ND) in ducks has been conducted at some areas in Indonesia and other countries by serology method and viral isolation. The result indicated that ND virus was capable of infecting ducks, stimulated immune response, with or without manifestation of the clinical signs of ND . Based on the pathotyping tests, viral isolates were considered as velogenic strains. Some infected ducks could spread the virus to the environment through faeces, so that it will be very dangerous for chicken breeder residing in the same location with many duck populations . On that account, ducks have an important role in spreading the ND virus, thus care should be taken on the presence of ducks in the poultry farms . Key word : Newcastle disease, ducks, velogenic strain
Marek's Disease in Chicken: I. Aetiology, Pathogenesis and its Control R M A Adjid; R Damayanti; H Hamid; T Sjafriati; Darminto .
WARTAZOA, Indonesian Bulletin of Animal and Veterinary Sciences Vol 12, No 2 (2002)
Publisher : Indonesian Center for Animal Research and Development

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (153.523 KB) | DOI: 10.14334/wartazoa.v12i2.768

Abstract

Marek’s disease (MD) is a neoplastic and neuropathic disease of poultry, especially chicken, caused by a highly contagious cell-associated herpesvirus. Marek’s disease is one of poultry diseases that is seriously concerned by poultry farmers. Before introduction of vaccine technology, the disease caused great economic loss in poultry industry, for example, in the United State the annual losses was estimated around $150 millions. Marek’s disease virus is relatively resistant to physical and chemical influence.  In  poultry  farms  the  virus  remains infectious  for  months  and  play  an  important  role  as  source of  infection. Pathogenesis of the disease is complex and influenced by factors. There are four phases of infection: early cytolytic infection, latent infection, second phase of cytolytic infection, and proliferative infection. Pathogenesis influenced by factors causes variety on clinical syndroms and incubation period of the disease in field condition. Successful disease control can be achieved, with vaccination as the main program, by selection of breed resistance and good management systems. The emergence of more virulent pathotypes of MD is a serious threat to the poultry industry, which requires development of more effective control strategies.   Key words. Marek, poultry, etiology, pathogenesis, control
Infectious Bronchitis (IB) Disease and its Control in Chicken Risa Indriani; Darminto .
WARTAZOA, Indonesian Bulletin of Animal and Veterinary Sciences Vol 9, No 2 (1999)
Publisher : Indonesian Center for Animal Research and Development

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (274.556 KB) | DOI: 10.14334/wartazoa.v9i2.723

Abstract

Infectious Bronchitis (IB) is an acute, highly contagious viral respiratory disease of chicken’s caracterized by tracheal rallies, coughing, sneezing and nasal discharge in young chicks. In addition, the disease may affect kidhney, and in laying flock there is usually a drop in egg production and quality. IB is a major negative economic importance in poultry industry because the disease causes poor weight gain and feed efficiency, mortality in young chicks, reduction in egg production and egg quality in laying flock. IB is distributed worldwide and has been reported to be present in Indonesia. IB is caused by virus of a member of Coronaviridae under genera of Coronavirus. Spreading of IB virus among chickens usually by inhalation. Diagnosis of the disease can be based on the isolation and identification of the virus using embryonated chicken eggs and trachea organ culture. There is no treatment available for IB, so the control of the disease is mainly by vaccination. The existence of multiple serotipes of IB virus requires vaccines which are represent the antigenic spectrum of field isolates. To ensure the results of vaccination program, monitoring antibody titers following vaccination is recommended. The most widely used serological test for antibody monitoring is an enzyme linked immunosorbent assay (ELISA) or Haemaglutination Inhibition (HI) test.   Key words: IB, virus, chicken, control
Zoonotic Diseases Associated with Encephalitis Darminto .; Sjamsul Bahri; Muharam Saepulloh
WARTAZOA, Indonesian Bulletin of Animal and Veterinary Sciences Vol 9, No 1 (1999)
Publisher : Indonesian Center for Animal Research and Development

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (338.897 KB) | DOI: 10.14334/wartazoa.v9i1.728

Abstract

Outbreak of encephalitis, which killed more than 80 people in Malaysia, invited public attention throughout the world, especially  stakeholders  in  animal husbandry  and  veterinary  practices.  The  disease  was initially  diagnosed as Japanese-B- encephalitis (JE) which was transmitted to human from infected pigs. Recently, the causal agent of the outbreak has been identified as morbilivirus which was called Hendra-like Virus or Nipah Virus. Indonesia as neighboring country to Malaysia needs to take anticipation to prevent the occurrence of similar outbreak. For anticipation, it is required to understand all diseases, which cause encephalitis, especially the zoonotic diseases. From many infectious diseases of animal, only 17 diseases which are able to cause encephalitis, 9 of them are zoonotic diseases: Eastern equine encephalomyelitis (EEE), Western equine encephalomyelitis   (WEE),   Venezuelan   equine   encephalomyelitis   (VEE),   Japanese-B-encephalitis   (JE),   Murray   valley encephalitis (MVE), Louping-ill, Rabies and Equine morbilivirus (EMV) and Nipah Virus. The viral agents and the mode of transmission to human are discussed in this publication. All those encephalitic zoonoses, except Rabies and JE, are exotic to Indonesia. So it is required to keep the free status of Indonesia to those diseases by strict quarantine measures. All imported animals to Indonesia should be confirmed free from those diseases based on the laboratory examination.   Key words: Encephalitis, zoonosis, nipah virus