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All Journal Medical Laboratory Technology Journal Meditory : The Journal of Medical Laboratory Jurnal Skala Kesehatan Jurnal Labora Medika Husada Mahakam: Jurnal Kesehatan Jaringan Laboratorium Medis Tropical Health and Medical Research Journal Jurnal Karya Generasi Sehat

Ratih Dewi Dwiyanti

Department Of Medical Laboratory Technology Poltekkes Kemenkes Banjarmasin

Author-ID : 2504739

Religion Biochemistry, Genetics & Molecular Biology Chemistry Dentistry Environmental Science Health Professions Immunology & microbiology Medicine & Pharmacology Nursing Public Health Other

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Contamination of Bacillus cereus in Elementary School Snack Food Ayu fahani; Ratih Dewi Dwiyanti; Ahmad Muhlisin
Tropical Health and Medical Research Vol. 1 No. 2 (2019): Tropical Health and Medical Research
Publisher : Baiman Bauntung Batuah Center

Many food snacks have the advantage but still have health risks as evidenced by the many cases of food poisoning, one of the causes of food poisoning is Bacillus cereus. This study aims to determine the amount and percentage of Bacillus cereus pollution on snacks sold in elementary schools. This research is expected to provide information to the public about the presence or absence of bacteria Bacillus cereus contamination. This research uses purposive sampling technique. The sample used in this study was hawker food sold in Elementary School as many as 20 samples consisting of 10 samples that have not fried and ten samples that have cooked. The results of the questionnaire study showed 83% of sellers did not put snacks in closed containers and found Bacillus cereus in meals. Conclusion 9 (45%) of food samples contaminated with Bacillus cereus bacteria

Growth of Malassezia furfur in Media with The Addition of Basil (Ocimum basilicum Linn) Powder Haris Nurdin Pratama; Ratih Dewi Dwiyanti; Ahmad Muhlisin
Tropical Health and Medical Research Vol. 2 No. 1 (2020): Tropical Health And Medical Research
Publisher : Baiman Bauntung Batuah Center

Malassezia furfur is very easy to infect the skin of people who are often in damp places with higher water content for a long time. One of the plants that can add to inhibit the growth of Malassezia furfur is basil. The purpose of this study was to determine the number of Malassezia furfur mushroom growths formed on the SDA media with the addition of basil powder concentrations of 2%, 4%, 6%, and 8% on day 5. The method used in this study was experimental with a post-test research design with control. The material used is the leaves of basil plants obtained from basil plantations in Sukamara Village, Landasan Ulin Banjarbaru, Indonesia. The results of the study, Malassezia furfur, grew in all concentrations with a creamy colony, smooth and soft texture with sizes between 1-2 mm and microscopically obtained hyphae and spores. The conclusion of this study, Malassezia furfur mushroom growth in all concentrations of 2%, 4%, 6%, and 8% with the results of the number of colonies in a row that is 798 colonies, 755 colonies, 661 colonies, 622 colonies. The results showed a decrease in the name of colonies, with each increase in concentration.

Garlic Extract (Allium sativum L.) Effectively Inhibits Staphylococcus aureus and Escherichia coli by Invitro Test Agnina Listya Anggraini; Ratih Dewi Dwiyanti; Anny Thuraidah
Tropical Health and Medical Research Vol. 2 No. 2 (2020): Tropical Health and Medical Research
Publisher : Baiman Bauntung Batuah Center

Infection is a disease caused by the presence of pathogenic microbes, including Staphylococcus aureus and Escherichia coli. Garlic (Allium sativum L.) has chemical contents such as allicin, alkaloids, flavonoids, saponins, tannins, and steroids, which can function as an antibacterial against Staphylococcus aureus and Escherichia coli. This study aims to determine the antibacterial properties of garlic extract powder against Staphylococcus aureus and Escherichia coli. This research is the initial stage of the development of herbal medicines to treat Staphylococcus aureus and Escherichia coli infections. The antibacterial activity test was carried out by the liquid dilution method. The concentrations used were 30 mg/mL, 40 mg/mL, 50 mg/mL, 60 mg/mL and 70 mg/mL. The results showed that the Minimum Inhibitory Concentration (MIC) against Staphylococcus aureus and Escherichia coli was 40 mg/mL and 50 mg / mL. Minimum Bactericidal Concentration (MBC) results for Staphylococcus aureus and Escherichia coli are 50 mg/mL and 70 mg/mL. Based on the Simple Linear Regression test, the R2 value of Staphylococcus aureus and Escherichia coli is 0.545 and 0.785, so it can be concluded that there is an effect of garlic extract powder on the growth of Staphylococcus aureus and Escherichia coli by 54.5% and 78.5%. Garlic (Allium sativum L.) extract powder has potential as herbal medicine against bacterial infections but requires further research to determine its effect in vivo.

Ethanol Extract Of Centella Asiatica (L.) Urban Leaves Effectively Inhibit Streptococcus pyogenes and Pseudomonas aeruginosa by Invitro Test Dini Aulia Azmi; Nurlailah Nurlailah; Ratih Dewi Dwiyanti
Tropical Health and Medical Research Vol. 2 No. 2 (2020): Tropical Health and Medical Research
Publisher : Baiman Bauntung Batuah Center

Streptococcus pyogenes and Pseudomonas aeruginosa are some of the causes of infectious diseases. Centella asiatica (L.) Urban has many benefits for humans, including overcoming fever, anti-bacterial, and anti-inflammatory. This study aims to determine the inhibition of Centella asiatica (L.) Urban leaves ethanol extract on the growth of Streptococcus pyogenes and Pseudomonas aeruginosa. This research is the initial stage of the development of herbal medicines to treat Streptococcus pyogenes and Pseudomonas aeruginosa infections. The independent variable was the concentration of ethanol extract of Centella asiatica (L.) Urban leaves and the dependent variable was the growth of Streptococcus pyogenes and Pseudomonas aeruginosa. The anti-bacterial activity test was carried out by the liquid dilution method. The concentrations used are 20%, 40%, 60%, 80%. 100% The results showed that the minimum inhibitory concentration (MIC) against Streptococcus pyogenes: 40% and Pseudomonas aeruginosa: 40%. Minimum bactericidal concentration (MBC) results for Streptococcus pyogenes: 60% and Pseudomonas aeruginosa: 60%. So it can be concluded that there is inhibition of the ethanol extract of Centella asiatica (L.) Urban leaves on the growth of Streptococcus pyogenes and Pseudomonas aeruginosa. Centella Asiatica (L.) Urban extract has potential as herbal medicine against bacterial infections but requires further research to determine its effect in vivo.

Bacterial Contamination that Causes Food Poisoning in Fruit Salads in Banjarbaru Indonesia Nurmalasari Nurmalasari; Ekrima Dayanti; Ahmad Muhlisin; Ratih Dewi Dwiyanti; Erfan Roebiakto
Tropical Health and Medical Research Vol. 3 No. 2 (2021): Tropical Health and Medical Research
Publisher : Baiman Bauntung Batuah Center

Fruit salad is one food combination that often causes food poisoning because it contains cheese and milk. This study aims to analyze the bacterial contamination that causes food poisoning in fruit salads sold in Banjarbaru, Indonesia, and the factors that play a role in such pollution. Food poisoning from fruit salad can be anticipated by knowing bacterial contamination and the factors that play a role in it. This study uses an observational method with a cross-sectional design. The sample in this study was fruit salads from 18 different producers sold in Banjarbaru, Indonesia. Fruit salad with criteria containing fruit, cheese, and mayonnaise. The bacteria that caused food poisoning to find in the fruit salad, namely Staphylococcus aureus, and Bacillus cereus. In conclusion, his research found the contamination of bacteria that cause food poisoning in Fruit Salad in Banjarbaru City with a percentage of 22 percent. The types of bacteria are Staphylococcus aureus (11 percent) and Bacillus cereus (11 percent). It recommends that salad sellers wash their hands before processing, use running water to wash fruit, and use secure salad packaging.

Analysis of Adolescent Hemoglobin Levels on Knowledge, Body Mass Index, and Menstrual Patterns Siti Mas'odah; Ratih Dewi Dwiyanti; Ricko Dharmadi Utama
Tropical Health and Medical Research Vol. 3 No. 2 (2021): Tropical Health and Medical Research
Publisher : Baiman Bauntung Batuah Center

Anemia can be related to several factors, including knowledge, body mass index, and menstrual patterns. Adolescent girls are a high-risk group who experience anemia. This study aims to analyze the hemoglobin levels of adolescent girls about knowledge, body mass index, and patterns menstrual. Information on the relationship between hemoglobin levels and these factors expects to be a reference for strategies to overcome anemia in adolescent girls. The sample of this research is the students of Martapura Indonesia vocational high school, totaling 45 people. Data collection includes weight and height measurements, hemoglobin examination (using Easy Touch GCHb), and questionnaires. Data analysis used bivariate analysis of chi-square test with 0.05 with 95% confidence degree. Results Respondents had fewer hemoglobin levels 47%, normal 53%. The body mass index value of respondents is less than 85%, normal 13%, excess 2%. The respondent's menstrual pattern obtained 26% abnormal data, 74% normal data. Knowledge of anemia of respondents with enough category 34%, good category 66%. The conclusion is that there is no relationship between hemoglobin levels and body mass index (p-value 0.281), menstrual patterns (p-value 0.073), and knowledge of anemia (p-value 0.402) in adolescent girls. Hemoglobin levels may more influence by the consumption pattern of foods containing iron, so it recommends that young women consume more iron-rich foods and avoid the simultaneous consumption of substances that inhibit their absorption, such as tea and coffee.

Fungicidal Effects of Chloroform Extract of Red Galangal (Alpinia purpurata (Vieill.) K. Sch) on the Growth of Trichophyton rubrum Nur Asy Syifa Azizah; Anny Thuraidah; Ratih Dewi Dwiyanti
Tropical Health and Medical Research Vol. 4 No. 1 (2022): Tropical Health and Medical Research
Publisher : Baiman Bauntung Batuah Center

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35916/thmr.v4i1.59

Trichophyton rubrum is the most common causative agent of anthropophilic dermatophytosis worldwide. Treatment of dermatophytosis can use natural ingredients. In Indonesia, several medicinal plants have been used, one of which is red galangal (Alpinia purpurata (Vieill.) K. Sch). This study aims to determine the effectiveness of red galangal rhizome chloroform extract on the growth of Trichophyton rubrum. This study is a True Experiment study with Posttest Only With Control Group Design consisting of 8 treatments namely positive control, extract control, 0 mg/mL (negative control), 20 mg/mL, 30 mg/mL, 40 mg/mL, 50 mg/ml, and 60 mg/mL extract of red galangal rhizome chloroform with 3 repetitions. The antifungal activity test was carried out using the determination of Minimum Inhibitory Concentration (MIC), Minimum Fungicidal Concentration (MFC), and Bioautography Test. The results showed that the red galangal rhizome chloroform extract in the Agar Overlay Bioautography Test contained an inhibition zone on the growth of Trichophyton rubrum. The determination of MIC and MFC were 20 mg/mL and 40 mg/mL, respectively. The study concludes that the red galangal rhizome chloroform extract affects the growth of the fungus Trichophyton rubrum. Research can be continued by knowing more specifics about the bioactive compounds from the red galangal rhizome, which have antifungal activity against Trichophyton rubrum.

Staphylococcus aureus pada keyboard di unit pelayanan penunjang rumah sakit Deby Arnisya Putri; Erpan Roebiakto; Ratih Dewi Dwiyanti
Jurnal Skala Kesehatan Vol 9 No 1 (2018): JURNAL SKALA KESEHATAN
Publisher : Politeknik Kementerian Kesehatan Banjarmasin

Computer keyboard can be a cause of health problems due to poor cleanliness. Keyboard that is not cleaned contains more bacteria. Staphylococcus aureus is one of the bacteria that causes nosocomial infection that can be found on the keyboard. The purpose of this research is to know the description of Staphylococcus aureus bacteria in Hospital Supporting Unit. This type of research is descriptive survey with 16 samples with total sampling. Based on research conducted on the keyboard in the Medical Support Unit Hospital found Staphylococcus aureus as much as 68.75% It is recommended to do keyboard cleanliness and cleanliness of computer users before and after using the computer.

Efektivitas Air Perasan Jeruk Nipis (Citrus aurantifolia) dalam Menghambat Pertumbuhan Escherichia coli Ratih Dewi Dwiyanti; hana nailah; Ahmad Muhlisin; Leka Lutpiatina
Jurnal Skala Kesehatan Vol 9 No 2 (2018): JURNAL SKALA KESEHATAN
Publisher : Politeknik Kementerian Kesehatan Banjarmasin

Lime (Citrus aurantifolia) can be used for cough medicine, sputum laxative, influenza, and acne medication. Lime contains useful chemical compounds, one of which is essential oils and flavonoids which function as antibacterials and play a very important role in inhibiting bacterial growth. The purpose of this study was to determine the effectiveness of lime juice (Citrus aurantifolia) at a concentration of 10%, 20%, 30%, 40%, 50%, 60%, 70%, 80%, 90% and 100% on the growth of Escherichia coli in vitro. This research is experimental with post test only control group design. The research sample was lime juice. Antibacterial power testing using well diffusion method. Antibacterial power parameters were determined by measuring the inhibitory zone (mm) formed around the growth of test bacteria on Muller Hinton Agar media. The results of the study showed that lime juice inhibition zones against the growth of Eschericia coli at concentrations of 40%, 50%, 60%, 70%, 80%, 90% and 100% were 7.25mm, 13.25mm, 14.25mm, 16mm, 17mm, 18.25mm, and 20.75mm. Based on linear regression test obtained significant value of 0.000 <α 0.05 so it can be concluded that there is an effect of lime juice on Eschericia coli growth in vitro. It is recommended that further research be carried out to test the inhibitory power of lime juice using other methods or against bacteria of other species.

Angka Kuman Es Batu Produksi Rumah Tangga Annisa Nur Yulianti; Ratih Dewi Dwiyanti; Wahdah Norsiah; Leka Lutpiatina
Jurnal Skala Kesehatan Vol 9 No 1 (2018): JURNAL SKALA KESEHATAN
Publisher : Politeknik Kementerian Kesehatan Banjarmasin

Air adalah kebutuhan dsar manusia, baik untuk keperluan sehari-hari seperti mandi, cuci, masak, dan minum. Air tidak hanya untuk air minun saja, tetapi dapat dibuat es batu yang memenuhi syarat bakteriologis. Penelitian bertujuan untuk mengetahui hasil TPC (Total Plate Count) pada es batu produksi rumah tangga di Kelurahan Sungai Besar. Penelitian ini bersifat survei deskriptif, sampel diperiksa dengan menggunakan metode TPC (Total Plate Count) dengan pengenceran 100 sampai 10-4. Sampel pemeriksaan berupa es batu yang diambil dari rumah produksi di Kelurahan Sungai Besar yang berjumlah 6. Hasil penelitian dari 24 sampel es batu produksi rumah tangga di Kelurahan Sungai Besar adalah 2 sampel yang memenuhi syarat dan 22 sampel tidak memenuhi syarat. Kesimpulan dari penelitian ini adalah 80% sampel tidak memenuhi syarat Standar Nasional Indonesia (SNI) 01-3553-2006. Saran penelitian lebih lanjut dengan melakukan identifikasi terhadap jenis bakteri yang terdapat dalam es batu tersebut.

Co-Authors Agnina Listya Anggraini Ahmad Muhlisin Aima Insana Aima Insana Aina Nurvita Sari Akhmad Muhlisin akhmad muntaha Amalia Amalia Annisa Nur Yulianti Anny Thuraidah Anny Thuraidah Ayu fahani Ayu Sutiany Deby Arnisya Putri Dini Aulia Azmi Dinna Rakhmina Dinna Rakhmina Ekrima Dayanti Erfan Roebiakto Erpan Roebiakto Fransiskus Xaverius Agung Pinto Laksono Haitami Haitami hana nailah Haris Nurdin Pratama Indah Kurnia Widiningsih leka lutpiatina, leka Neni Oktiyani Nur Asy Syifa Azizah Nur Lailah Lailah Nur Rizka Adriana Nur Rizqi Amaliah Nurlailah Nurlailah Nurlailah Nurlailah Nurmalasari Nurmalasari Nurul Huda Rahmayunita Rahmayunita Rahmayunita Rifqoh Rifqoh Rima Putri Wulandari Rion Dediq Siti Mas'odah Utama, Ricko Dharmadi wahdah norsiah Yuli Susanti

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