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All Journal Medical Laboratory Technology Journal Meditory : The Journal of Medical Laboratory Jurnal Skala Kesehatan Jurnal Labora Medika Husada Mahakam: Jurnal Kesehatan Jaringan Laboratorium Medis Tropical Health and Medical Research Journal Jurnal Karya Generasi Sehat
Ratih Dewi Dwiyanti
Department Of Medical Laboratory Technology Poltekkes Kemenkes Banjarmasin
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DAYA HAMBAT EKSTRAK DAUN KENIKIR (Cosmos caudatus Kunth.) TERHADAP Staphylococcus aureus Leka Lutpiatina; Nur Rizqi Amaliah; Ratih Dewi Dwiyanti
Meditory : The Journal of Medical Laboratory Vol 5, No 2 (2017): meditory ,Volume 5, No 2, Desember 2017
Publisher : Jurusan Analisis Kesehatan, Poltekkes Kemenkes Denpasar

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (247.129 KB) | DOI: 10.33992/m.v5i2.116

Abstract

Daun kenikir (Cosmos caudatus Kunth.) sudah lama dimanfaatkan masyarakat untuk dikonsumsi maupun pengobatan tradisional. Daun kenikir memiliki kandungan fenol, flavonoid, saponin dan tanin yang berfungsi sebagai antibakteri. Penelitian ini bertujuan untuk mengetahui konsentrasi hambat minimal dan konsentrasi bunuh minimal ekstrak daun kenikir terhadap bakteri Staphylococcus aureus secara in vitro. Penelitian ini bersifat true eksperiment dengan rancangan postest only control group design. Variabel bebas dalam penelitian ini adalah konsentrasi ekstrak daun kenikir (Cosmos caudatus Kunth.).  Variabel terikat dalam penelitian ini adalah bakteri Staphylococcus aureus. Hasil penelitian Konsentrasi Hambat Minimal (KHM) menunjukkan kekeruhan pada konsentrasi 160 mg/ml, 320 mg/ml dan adanya kejernihan pada konsentrasi 340 mg/ml, 380 mg/ml dan 400 mg/ml. Hasil penelitian Konsentrasi Bunuh Minimal (KBM) diperoleh jumlah koloni pada konsentrasi 160 mg/ml sebanyak 60 CFU/ml, 320 mg/ml sebanyak 12 CFU/ml, 340 mg/ml sebanyak 3 CFU/ml, 380 mg/ml sebanyak 0 CFU/ml dan 400 mg/ml sebanyak 0 CFU/ml. Berdasarkan hasil penelitian dapat disimpulkan bahwa KHM ekstrak daun kenikir adalah 340 mg/ml dan KBM ekstrak daun kenikir adalah 380 mg/ml. Disarankan dilakukan penelitian lebih lanjut mengenai pengaruh ekstrak daun kenikir terhadap bakteri Staphylococcus aureus adalah dengan menggunakan pelarut lain.
Screening of Sengkuang (Dracontomelon dao (Blanco) Merr. & Rofe) as Antibacterial Escherichia coli Ratih Dewi Dwiyanti; Nurlailah Nurlailah
Medical Laboratory Technology Journal Vol. 8 No. 1 (2022): June
Publisher : Poltekkes Kemenkes Banjarmasin Jurusan Analis Kesehatan

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (236.077 KB) | DOI: 10.31964/mltj.v0i0.420

Abstract

Dracontomelon dao (Blanco) Merr. & Rofe has antimicrobial potential, but Dracontomelon dao (Blanco) Merr. & Rofe, originating from Kalimantan, Indonesia, has not been tested for its antibacterial properties and secondary metabolite content. This study aimed to analyze the secondary metabolite content of various Dracontomelon dao (Blanco) Merr leaf extracts. & Rofe and their antibacterial effectiveness against Escherichia coli. The study used 96% ethanol extract, ethyl acetate, petroleum ether, and chloroform by maceration simplicia leaves. Dracontomelon dao (Blanco) Merr. & Rofe is from South Kalimantan, Indonesia. Antibacterial testing was carried out using the agar diffusion method, the Minimum Inhibitory Concentration (MIC) method, and the Minimum Bactericidal Concentration (MBC) method. The results showed that the triterpenoid content was the highest in all extracts, namely ethanol extract 669.8 ± 2,000 mg/ml, ethyl acetate 90 ± 7,638 mg/ml, petroleum ether 792, 800 ± 4,583 mg/ml, chloroform 615, 467 ± 0,577 mg/ml. ml. The antibacterial test using the well diffusion method showed the best inhibition of bacterial growth in ethanol extract, with the largest inhibition zone of 24 mm at a concentration of 500 mg/ml. The study's conclusion was the extract of ethanol, ethyl acetate, petroleum ether and chloroform from the leaves of Dracontomelon dao (Blanco) Merr. & Rofe contains the same five secondary metabolites: flavonoids, alkaloids, saponins, tannins and triterpenoids. All types of extracts showed varying abilities to inhibit the growth of Escherichia coli. Further research is needed on the antibacterial properties of Dracontomelon dao (Blanco) Merr extract. & Rofe, especially on the active substance triterpenoid, which is the largest content in all types of extracts.
Pengaruh Lama Penyimpanan Jamu Beras Kencur terhadap Pertumbuhan Kapang Fransiskus Xaverius Agung Pinto Laksono; Ratih Dewi Dwiyanti; Rifqoh Rifqoh; Aima Insana
Jaringan Laboratorium Medis Vol 4, No 2 (2022): November 2022
Publisher : Poltekkes Kemenkes Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31983/jlm.v4i2.8571

Abstract

During the Covid-19 pandemic, people began to maintain their health, by using herbal medicine as an alternative treatment. One type of herbal medicine used is jamu beras kencur because it has properties to increase stamina, increase appetite, and relieve aches and cough disorders. In the Regulation of the Minister of Health of the Republic of Indonesia No. 007 of 2012 carrying herbs such as jamu beras kencur does not require marketing authorization. The quality of the herbal medicine produced has not been confirmed as feasible from microbial contamination, especially in molds, the number of yeast molds in the jamu beras kencur must comply with BPOM standards Number 32 of 2019 namely AKK 103 colonies/mL. This study aimed to determine the effect of the storage time of jamu beras kencur on mold growth with storage treatments of 0 hours, 4 hours, 8 hours, 12 hours, and 24 hours at room temperature. This type of research is a Quasi Experiment with a research design of One Group Pretest-Postest Design. The results of this study showed that the average AKK value was 0 hours 0.2x100 colonies/mL, 4 hours 0.4x100 colonies/mL, 8 hours 0.4x100 colonies/mL. 12 hours 0.8x100 colonies/mL and 24 hours 2.2x100 colonies/mL. These results stated that the jamu beras kencur met the BPOM standard requirements Number 32 of 2019 which was AKK 103 Colonies/mL. The results of the Kruskal Wallis test obtained a sig value of 0.286, it was concluded that there was no effect of storage time for the jamu beras kencur on the growth of molds. The identification results on the jamu beras kencur obtained fungal species, namely Aspergillus flavus, Aspergillus niger, and Penicillium sp. Suggestions for further researchers can examine the effect of storage time on mold growth in other types of herbal medicine.
Efektivitas Ekstrak Etanol Daun Jeruk Sambal (Cytrus amblycarpa (Hassk) Ochse) Sebagai Biolarvasida Terhadap Mortalitas Larva Nyamuk Aedes aegypti Rahmayunita Rahmayunita Rahmayunita; Rifqoh Rifqoh Rifqoh; Nur Lailah Lailah; Ratih Dewi Dwiyanti
Jurnal Labora Medika Vol 6, No 2 (2022): Jurnal Labora Medika
Publisher : Program Studi Teknologi Laboratorium Medik

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26714/jlabmed.6.2.2022.33-35

Abstract

Pengendalian vektor Aedes aegypti dalam pemberantasan larva berupa larvasida berbahan kimia dapat menimbulkan efek negatif seperti resistensi serta berbahaya bagi lingkungan dan manusia jika terus menerus digunakan sehingga diperlukan alternatif biolarvasida salah satunya daun jeruk sambal. Tujuan penelitian untuk mengetahui efektivitas ekstrak etanol daun jeruk sambal sebagai biolarvasida terhadap mortalitas larva nyamuk Aedes aegypti. Jenis penelitian adalah eksperimen dengan rancangan Posttest With Control Group Design. Subjek penelitian yang digunakan adalah larva nyamuk Aedes aegypti sebanyak 700 ekor. Hasil penelitian menunjukan  kematian larva nyamuk Aedes aegypti setelah terpajan 24 jam ekstrak daun jeruk sambal konsentrasi 0,5%, 1%, 1,5%, 2%, dan 2,5% secara berturut-turut adalah 48%, 58%, 71%, 87%, dan 100%. Setelah terpajan 48 jam jumlah kematian larva secara keseluruhan adalah 100%. Berdasarkan hasil uji regresi yang dilakukan didapatkan efektifitas ekstrak daun jeruk sambal sebesar 83,8%, dan nilai LC90 didapatkan pada konsentrasi 0,181802%. Sehingga disimpulkan bahwa ekstrak etanol daun jeruk sambal (Cytrus amblycarpa) efektif terhadap kematian larva nyamuk Aedes aegypti. Bagi peneliti selanjutnya, disarankan melakukan penelitian dengan pelarut yang berbeda seperti pelarut non polar dengan menggunakan metode ekstraksi yang lain.
Efektivitas Ekstrak Etanol Daun Jambu Biji Merah (Psidium guajava L.) Sebagai Larvasida Terhadap Mortalitas Larva Aedes aegypti Nur Rizka Adriana; Rifqoh Rifqoh; Dinna Rakhmina; Ratih Dewi Dwiyanti
Jurnal Labora Medika Vol 6, No 2 (2022): Jurnal Labora Medika
Publisher : Program Studi Teknologi Laboratorium Medik

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26714/jlabmed.6.2.2022.36-40

Abstract

ABSTRACT  The vector control of Aedes aegypti mosquito that causes DHF is carried out by chemical control of the larvae. Resistance can occur if used repeatedly over a long time. Another alternative that can be used is with natural ingredients such as red guava leaves because they have secondary metabolites that can be used as larvicides. This study aimed to determine the effectiveness of red guava leaf extract (Psidium guajava L.) as a larvicide on mortality of Aedes aegypti and Lethal Concentration 50 and 90 from ethanol extract of red guava leaves. This type of research is a quasi-experimental and posttest with control group design. The test material used was 25 larvae of Aedes aegypti instar III each treatment and the extract was obtained using the maceration extraction method. The concentration variation is 8%; 9%; 10%; 11%; 12% with 4 repetitions. The results obtained that the average mortality of larvae at a concentration of 8% was 13 tails (51%); 9% 18 tails (72%); 10% 22 tails (88%); 11% 23 tails (92%), 12% 25 tails (100%). The Regression test results showed the effect of adding extract was 77.9% at a 24-hour exposure time. The results of the Probit test for the LC50 value obtained a concentration of 24.0824 ppm and LC90 of 32.0479 ppm. Keywords: Ethanol Extract of Guava Leaves, Aedes aegypti Larvae, Lethal Concentration 50, Lethal Concentration 90.  ABSTRAK Pengendalian vektor nyamuk Aedes aegypti penyebab DBD dilakukan dengan pengendalian larva secara kimiawi. Resistensi dapat terjadi jika digunakan berulang dalam jangka waktu yang panjang. Alternatif lain yang dapat digunakan yaitu dengan bahan alami seperti daun jambu biji merah karena memiliki senyawa metabolit sekunder yang dapat digunakan sebagai larvasida. Tujuan penelitian ini untuk mengetahui efektivitas dari ekstrak daun jambu biji merah (Psidium guajava L.) sebagai larvasida terhadap mortalitas larva Aedes aegypti dan Lethal Concentration 50 dan 90 dari ekstrak etanol daun jambu biji merah. Jenis penelitian ini adalah Quasi eksperiment dan rancangan Posttest With Control Group Design. Bahan uji yang digunakan adalah larva Aedes aegypti instar III sebanyak 25 ekor setiap perlakuan dan ekstrak daun jambu biji merah dengan metode ekstraksi maserasi. Variasi konsentrasi yaitu 8%; 9%; 10%; 11%; 12% dengan 4 kali pengulangan. Hasil penelitian yang didapatkan rata-rata kematian larva pada konsentrasi 8% yaitu 13 ekor (51%); 9% 18 ekor (72%); 10% 22 ekor (88%); 11% 23 ekor (92%), 12% 25 ekor (100%). Hasil uji Regresi menunjukkan pengaruh penambahan ekstrak sebesar 77,9% pada waktu pemaparan 24 jam. Hasil uji Probit nilai LC50 didapatkan konsentrasi sebesar 24,0824 ppm dan LC90 sebesar 32,0479 ppm. Kata Kunci: Ekstrak Etanol Daun Jambu Biji Merah, Larva Aedes aegypti, Lethal Concentration 50, Lethal Concentration 90
KARAKTERISTIK DAN PEMERIKSAAN RAPID TES ANTIBODI COVID-19 PADA IBU HAMIL DI PUSKESMAS WILAYAH MARTAPURA Dinna Rakhmina; Aina Nurvita Sari; Ratih Dewi Dwiyanti
Husada Mahakam Vol 11 No 2 (2021): Nopember 2021
Publisher : Poltekkes Kemenkes Kalimantan Timur (URL: http://poltekkes-kaltim.ac.id/)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35963/hmjk.v11i2.258

Abstract

Wuhan, China was the place where the first cases of Covid-19 were found, and 118 pregnant women with COVID-19 were identified from 50 hospitals throughout the city of Wuhan from December 2019 – March 2020. The purpose of this study was to find out the description of the Covid-19 examination in pregnant women at Puskesmas Martapura City. A descriptive survey is conducted using a cross-sectional design. All pregnant women in their third trimester who had a rapid test for Covid-19 antibodies performed at the Puskesmas Martapura City and samples taken utilizing a full sampling approach were included in this study. The study's conclusions were mostly based on their age (20-35 years) 14 individuals (70%), senior high school 12 people (60%), housewives 18 people (90%), and knowledge level (100%). The results showed that 131 people were reactive and 957 were non-reactive in the antibody rapid test examination for pregnant women in their third trimester from June 2020 to March 2021. According to the findings, 12.04% of pregnant women in their third trimester are reactive, whereas 87.96% are non-reactive. Suggestions to the public on how to better understand clinical symptoms, mechanisms of transmission, and Covid-19 prevention efforts in order to encourage good lifestyle choices. This research can be continued by observing the severity of Covid-19 symptoms in pregnant women who are infected and the effect of pregnant women infected with Covid-19 on babies born. Keywords: Covid-19, Antibody rapid test, Pregnant woman
Perbandingan Nilai Laju Endap Darah Dengan Metode Automatik Dan Metode Westergren Pada Pasien Tuberkulosis Rima Putri Wulandari; Ahmad Muhlisin; Wahdah Norsiah; Ratih Dewi Dwiyanti; Aima Insana
Jurnal Karya Generasi Sehat Vol. 1 No. 1 (2023): Edisi Desember Tahun 2023
Publisher : Poltekkes Kemenkes Banjarmasin

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31964/jkgs.v1i1.81

Abstract

Pada pasien tuberkulosis terjadi peningkatan LED yang disebabkan oleh inflamasi yang menyebabkan eritrosit rouleaux mengendap dengan cepat sehingga nilai LED tinggi.Ada beberapa metode manual yang digunakan dalam pemeriksaan LED, salah satu metode manual yang dianjurkan oleh International Committee for Standardisation in Hematology (ICSH) adalah metode Westergren. Seiring perkembangan yang ada metode otomatis menjadi pemeriksaan LED yang digunakan untuk menghindari atau mengurangi resiko paparan petugas laboratorium terhadap kontaminasi agen infeksi dengan prinsip kerja memakai infrared yang memungkinkan membantu kecepatan pengendapan sel darah dengan waktu pemeriksaan 2 kali lebih cepat. Penelitian ini bertujuan untuk mengetahui bagaimana perbandingan nilai laju endap darah dengan metode automatik dan metode westergren pada pasien tuberkulosis. Jenis penelitian ini adalah survey analitik dengan rancangan study komparatif. Populasi penelitian ini adalah seluruh penderita tuberkulosis di Wilayah Puskesmas Cempaka yang berjumlah 36 orang. Hasil pemeriksaan LED pada pasien tuberkulosis dengan metode automatik memiliki rata-rata 36.97 mm/jam, pada metode westergren memiliki rata-rata 38.53 mm/jam. Berdasarkan uji T Dependent menunjukkan bahwa nilai p value 0.003 yang artinya terdapat perbedaan yang bermakna antara nilai laju endap darah dengan metode automatik dan metode westergren pada pasien tuberkulosis.
Co-Authors Agnina Listya Anggraini Ahmad Muhlisin Aima Insana Aima Insana Aina Nurvita Sari Akhmad Muhlisin akhmad muntaha Amalia Amalia Annisa Nur Yulianti Anny Thuraidah Anny Thuraidah Ayu fahani Ayu Sutiany Deby Arnisya Putri Dini Aulia Azmi Dinna Rakhmina Dinna Rakhmina Ekrima Dayanti Erfan Roebiakto Erpan Roebiakto Fransiskus Xaverius Agung Pinto Laksono Haitami Haitami hana nailah Haris Nurdin Pratama Indah Kurnia Widiningsih leka lutpiatina, leka Neni Oktiyani Nur Asy Syifa Azizah Nur Lailah Lailah Nur Rizka Adriana Nur Rizqi Amaliah Nurlailah Nurlailah Nurlailah Nurlailah Nurmalasari Nurmalasari Nurul Huda Rahmayunita Rahmayunita Rahmayunita Rifqoh Rifqoh Rima Putri Wulandari Rion Dediq Siti Mas'odah Utama, Ricko Dharmadi wahdah norsiah Yuli Susanti