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PRODUKSI BIODIESEL DARI BIOMASSA Chlamydomonas sp. ICBB 9113 DIKULTIVASI MENGGUNAKAN MEDIA YANG MURAH: EFEKTIFITAS DARI BEBERAPA METODE EKSTRAKSI Patmawati Patmawati; Bustami Ibrahim; Iriani Setyaningsih; Untung Sudadi
Widyariset Vol 17, No 2 (2014): Widyariset
Publisher : Pusbindiklat - LIPI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (344.292 KB) | DOI: 10.14203/widyariset.17.2.2014.269-276

Abstract

The main challenges to overcome in biodiesel production from microalgae are lower oil yield, as compared to those derived from plant and animal biomass, and expensive culture media. This work was aimed to compare the effectivity of three extraction methods differed in solvent used, e.g. n-hexane (N-hex), ethanol (Eth), and mixture of chloroform-methanol-water (CMW), to extract crude lipid and biodiesel from dry biomass of Chlamydomonas sp. ICBB 9113. This microalgae was cultivated in a cheap culture media using N and P soil fertilizers as nutrient sources. The results showed that, by using N-hex, Eth, and CMW methods, it could be extracted, respectively, 0.06%, 4.51%, and 20.45% crude lipid, and 384.2, 1333.8, and 2430.6 mg/100g biodiesel. The fatty acid profile of the studied microalgae biomass was: C8:0 (0.11%), C10:0 (0.09%), C14:0 (7.70%), 16:0 (1.39%), C18:0 (0.85%), C14:1 (5.12%), C16:1 (7.09%), C18:1 (8.28%), C18:2 (12.80%), and C18:3 (42.57%). Fatty acid characterization showed that Chlamydomonas sp. ICBB 9113 was dominated by C18: 3 and C16:0. Therefore, these microlagae were suitable to be used as raw material for biodiesel production to substitute the conventional fuel.
The Influence of Harvesting Period on Lipid Associated Antioxidant Activity of Semicontinuously Grown Chlorella vulgaris Tjandara Chrismadha; Diani Sartika; Iriani Setyaningsih; Uju Uju
ANNALES BOGORIENSES Vol 14, No 1 (2010): Annales Bogorienses
Publisher : Research Center for Biotechnology - Indonesian Institute of Sciences (LIPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (522.466 KB) | DOI: 10.14203/ann.bogor.2010.v14.n1.25-30

Abstract

A green alga,  Chlorella vulgaris  was grown semi-continuosly at various harvesting periods, and the lipid content and its associated antioxidant activity was examined. The harvesting periods were 9, 18, and 27 days, while the algal culture was placed  in 10 L glass  bottles provided with aeration  for gas exchange and  mixing. Light  was  obtained  from  440  watt  cool  fluorescent  lamps  placed  at  one  side  of  the  culture.  Room temperature was  26-32C.  Algal  lipid  extraction  was  done  based  on  liquid  phase  separation  of  methanol :chloroform:water  and  the  antioxidant  activity  was  examined  by  means  of  oxidation  inhibitory  in ethanol emulsified  limnoleic  acid.  In  addition,  a  TLC  analysis  was  performed  to  identify  the  antioxidant compouns soluble  in  the  lipid.  The  results  showed  that  harvesting  per iod  had  a  significant  influence  on  the algal lipid  content,  which  were  11.94,  12.96,  and  16.51%  of  the  dry  weight  in  the  culture  with  harvesting periods of 9, 18, and 27 days, respectively. No remarkable effect of the  harvesting period on the  antioxidant activity, which were observed to inhibit oxidation of linoleic acid up to 67-71%. There were five compounds found can  be associated   with  the  algal  antioxidant  activity,  which  were  pheophorbide -a,  chlorophyll-b,chlorophyll-a, phaeophytin-a, -carotene, and an unidentified one. Key words: microalgae, Chlorella vulgaris, lipid, antioxidant, harvesting period 
Penilaian Risiko Semikuantitatif Logam Berat pada Ikan Salmon di Jabodetabek: Semi-Quantitative Risk Assessment of Heavy Metals in Salmon Distributed in Jabodetabek Nindya Hambar Wasisto; Wini Trilaksani; Iriani Setyaningsih
Jurnal Pengolahan Hasil Perikanan Indonesia Vol 25 No 2 (2022): Jurnal Pengolahan Hasil Perikanan Indonesia 25(2)
Publisher : Masyarakat Pengolahan Hasil Perikanan Indonesia (MPHPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17844/jphpi.v25i2.40550

Abstract

Salmon imports continue to rise, while there were indications in some waters of the salmon's origin country that is a potential danger of heavy metals contamination. This phenomenon raises awareness regarding the food safety of salmon consumption, therefore a risk assessment is crucial to be conducted. The purposes of this study were to determine the content of Hg, Pb, and Cd in salmon meat and to assess the risk of heavy metals in salmon consumption in Jabodetabek area. Salmon fish, 132 in total was randomly taken from Tanjung Priok Port, the heavy metal analysis used the Flameless Inductively Coupled Plasma Mass Spectrometry instrument, risk assessment based on the United States Environmental Protection Agency method and risk ranger based on Ross and Sumner, supported by systematic literature review and questionnaires. The heavy metals Hg, Pb, and Cd in salmon were detected at 0.0646±0.0056, 0.0505±0.0446, and 0.0119±0.0006 ppm, respectively, indicating that there were still below the thresholds set by Food and Drug Supervisory Agency of the Republic of Indonesia (0.5;0.2;and 0.1 ppm). The results of the semi-quantitative risk assessment for Hg, Pb, and Cd were 0.0567, 0.0013, and 0.0010 (less than 1) implying that salmon consumption have no a potential risk of hazard. However heavy metals are accumulated in human body, hence, the diet consumption analysis needed to be performed. The risk ranger showed that the estimated risk ranking of heavy metals was in the medium category. Based on the results, it can be concluded that imported and consumed salmon by Jabodetabek residents was still relatively safe.
Fraksinasi Flavonoid Spirulina platensis dengan Metode Kromatografi Lapis Tipis dan Aktivitas Inhibisi Enzim α-Glukosidase Hartoyo Notonegoro; Heder Djamaludin; Iriani Setyaningsih; Kustiariyah Tarman
Jurnal Kelautan Tropis Vol 25, No 3 (2022): JURNAL KELAUTAN TROPIS
Publisher : Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jkt.v25i3.13905

Abstract

Spirulina platensis is a type of Cyanobacterium microalgae that forms multicellular helicoidal filaments. Spirulina platensis contains primary and secondary metabolites. The type and amount of the active compound Spirulina platensis depends on the method of extraction, fractionation, and isolation. So far, there are not much research data related to the active compound of Spirulina platensis extract cultured on Walne. The purpose of this study was to use of thin layer chromatography (TLC) method to separate flavonoids from Spirulina platensis biomass extract cultured on Walne media and the activity of a-glucosidase enzyme inhibition using biomass, crude extract, active fraction of flavonoids and phycocyanins from Spirulina platensis. This research method is descriptive experimental, which the Spirulina platensis is cultured on 80 g/L NaNO3 modified Walne media, extracted by maceration, fractionated by TLC and isolated the active compound by Preparative TLC (PTLC). The analysis included fraction and isolation of flavonoids from Spirulina platensis. The results showed that the TLC could be used to identify the active compound of Spirulina platensis extract cultured on Walne. Fractionation of Spirulina platensis extract using stationary phase silica gel Si 60 GF254 and the best mobile phase with a combination of chloroform:ethyl acetate (6:4) and an Rf1 value of 0.58; Rf2 0.71; and Rf3 0.83, as well as yellow-orange spots. Isolation of the active compound of Spirulina platensis extract using PLTC stationary phase silica gel Si 60 PF254 and the best mobile phase combination eluent chloroform:ethyl acetate (9:1). RF value of Rf2 0.57; Rf3 0.86; and Rf4 0.93 with dark yellow-brown spots. The color of the spots from the PLTC results shows that the active compounds of Spirulina platensis extract are flavonoid compounds. Biomass, crude extract, phycocyanine extract and flavonoids from Spirulina do not have inhibitory activity against α-glucosidase enzyme.  Spirulina platensis merupakan jenis mikroalga Cyanobacterium yang membentuk filamen helicoidal multiseluler. Spirulina platensis mengandung senyawa metabolit primer dan sekunder. Jenis dan jumlah senyawa aktif Spirulina platensis tergantung pada metode ekstraksi, fraksinasi dan isolasi. Sejauh ini belum banyak data hasil penelitian terkait senyawa aktif ekstrak biomassa Spirulina platensis yang dikultur pada media Walne. Tujuan penelitian ini yaitu penggunaan metode kromatografi lapis tipis (KLT) untuk memisahkan flavonoid ekstrak biomassa Spirulina platensis yang dikultur pada media Walne serta aktivitas inhibisi enzim α-glukosidase menggunakan biomassa, ekstrak kasar, fraksi aktif flavonoid dan fikosianin dari Spirulina platensis. Metode penelitian ini adalah eksprimental deksriptif dimana mikroalga Spirulina platensis dikultur pada media Walne modifikasi 80 g/L NaNO3, diekstraksi dengan maserasi, difraksinasi dengan KLT dan diisolasi senyawa aktif dengan KLT Preparatif (KLTP). Analisis yang dilakukan meliputi analisis fraksi dan isolasi flavonoid dari Spirulina platensis. Hasil penelitian menunjukkan metode KLT dapat digunakan untuk mengindetifikasi senyawa aktif ekstrak Spirulina platensis yang dikultur pada media Walne. Fraksinasi ekstrak Spirulina platensis menggunakan fase diam silika gel Si 60 GF254 dan fase gerak kombinasi eluen terbaik kloroform:etil asetat (6:4) dan nilai Rf1 0,58; Rf2 0,71; dan Rf3 0,83, serta bercak berwarna kuning-oranye. Isolasi senyawa aktif ekstrak Spirulina platensis menggunakan KLTP fase diam silika gel Si 60 PF254 dan fase gerak kombinasi eluen terbaik kloroform:etil asetat (9:1). Nilai Rf2 0,57; Rf3 0,86; dan Rf4 0,93 dengan bercak berwarna kuning-cokelat gelap. Warna bercak hasil KLTP menunjukkan komponen senyawa aktif ekstrak Spirulina platensis berupa senyawa golongan flavonoid. Biomassa, ekstrak kasar, ekstrak fikosianin dan flavonoid dari Spirulina tidak memiliki aktivitas penghambatan terhadap enzim α-glukosidase.
In Vitro and In Vivo Malondialdehyde Inhibition Activities of Stichopus hermanii and Spirulina platensis Mega Safithri; Kustiariyah Tarman; Iriani Setyaningsih; Yanti Fajarwati; Imanniar Yuta Ellana Dittama
HAYATI Journal of Biosciences Vol. 29 No. 6 (2022): November 2022
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.29.6.771-781

Abstract

Previous research showed that Stichopus hermannii and Spirulina platensis had an antioxidant activity. It is indicated by the reduced malondialdehyde (MDA) level in the liver of the diabetic rats. However, the STZ administration did not significantly increase MDA concentration of diabetic rats' blood serum for 14 days. This research aimed to determine in vitro and in vivo MDA inhibition of S. hermanii and S. platensis. The in vitro antioxidant activity test was conducted using the MDA-TBA method, and a positive control used α-tocopherol. For in vivo experiment, diabetic rats (DM) were induced by streptozotocin for 21 days. Twenty-five rats which were divided into five groups: normal rat group (NA), diabetic rat group (DA), diabetic rat group + glibenclamide (DG), diabetic rats + Stichopus hermanii (SH), and diabetic rats + Spirulina platensis (SP). The in vitro results showed that the antioxidant activity of 25 ppm Spirulina platensis had the same MDA inhibitory activity as 200 ppm α-tocopherol, but 200 ppm Stichopus hermanii had lower inhibition than 200 ppm α-tocopherol. The in vivo result showed that Stichopus hermanii treatment was more effective in suppressing blood serum MDA concentration, but Spirulina platensis was more effective in suppressing liver MDA concentration.
Karakteristik fisikokimia tablet berbasis mikrokapsul minyak mata tuna dan spirulina: Evaluation of physicochemical characteristics of tuna’s eye oil microcapsule-spirulina tablets Fahri Sinulingga; Wini Trilaksani; Iriani Setyaningsih
Jurnal Pengolahan Hasil Perikanan Indonesia Vol 27 No 1 (2024): Jurnal Pengolahan Hasil Perikanan Indonesia 27(1)
Publisher : Department of Aquatic Product Technology IPB University in collaboration with Masyarakat Pengolahan Hasil Perikanan Indonesia (MPHPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17844/jphpi.v27i1.49473

Abstract

Omega-3 is very important for preventing the effects of decreased intelligence. The limitation of omega-3 is that it is highly susceptible to oxidation, which necessitates the inclusion of additional substances with antioxidant properties. Spirulina platensis can be used to prevent the oxidation of omega-3 in the form of dry dosages. The objective of this study was to identify the optimal formulation for incorporating microcapsules of tuna eye oil and S. Platensis in the production of tablets by considering physicochemical parameters, peroxide value, and water activity. The present study encompasses three distinct phases: extraction and encapsulation of tuna eye oil, cultivation of S. platensis, and formulation of tablets using the compression method. Treatments involving the tablet formula were classified into four distinct categories: F1 (containing 300 mg of tuna eye oil microcapsules and 140 mg of vitamin C), F2 (comprising 280 mg of tuna eye oil microcapsules and 160 mg of Spirulina culture), F3 ( 300 mg of tuna eye oil microcapsules and 140 mg of Spirulina culture), and F4 (containing 300 mg of tuna eye oil microcapsules and 140 mg of commercial Spirulina). The examination demonstrated that the eye oil derived from tuna satisfied the criteria for fish oil quality, with an acid value of 0.26±0.01 mg KOH/g, a peroxide value of 4.07±0.25 meq/kg, an anisidine value of 8.21±0.15 meq/kg, and a total oxidation value of 16.35±0.18 meq/kg, as well as a microencapsulation efficiency of 91.14%. The cultivation method employed in this study also ensured that the resulting Spirulina met the acceptable quality standards. The dry Spirulina had a water content of 9.02±0.07%, an ash content of 6.24±0.06%, a protein content of 57.55±0.21%, a fat content of 2.07±0.02%, and a carbohydrate content of 25.12±0.16%. The F3 tablet formulation was the most effective treatment, with physical properties that met the standards for tablet quality. It has a firmness value of 0.55%, disintegration time of less than 12 min, and fat content of 13.57%. The F3 formula demonstrated greater stability with respect to peroxide value and water activity during the storage period than the other formulas.
Perubahan kimiawi dan mikrobiologis selama fermentasi bekasam ikan nila menggunakan starter tunggal dan campuran: Change of chemical and microbiological during the fermentation of bekasam nile tilapia fish using single and mixed starters Desniar Desniar; Iriani Setyaningsih; Ike Marta Fransiska
Jurnal Pengolahan Hasil Perikanan Indonesia Vol 26 No 3 (2023): Jurnal Pengolahan Hasil Perikanan Indonesia 26 (3)
Publisher : Department of Aquatic Product Technology IPB University in collaboration with Masyarakat Pengolahan Hasil Perikanan Indonesia (MPHPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17844/jphpi.v26i3.50664

Abstract

Bekasam is a fermented fish product that tastes sour. Bekasams commonly employ freshwater fish, carbohydrate sources, and salt, which are left to ferment spontaneously for a period of 5-10 days. The quality of the bekasam produced was not uniform. This study aimed to investigate the microbiological and chemical properties of bekasam during the fermentation process using a single and mixed starter, and to identify the optimal treatment for the selected starter in terms of its chemical composition and sodium chloride content. The addition of 7.5% (weight-to-weight) salt and 25% (weight-to-weight) dry rice to the prepared Nile tilapia fish was followed by sterilization at 100 °C for 30 min.The sterilized fish mixture was subjected to treatments involving the incorporation of a single starter culture containing Lactobacillus plantarum SK (5) at a concentration of 10% (v/b), as well as a mixed starter culture consisting of L. plantarum SK (5) and Pediococcus pentosaceus BP (20) at a concentration of 10% (v/w). Undertaking the fermentation of Bekasam was fermented for an interval of 8 days every 2 days, encompassing the quantification of total Lactic Acid Bacteria (LAB), pH, and Total Acid Titratable (TAT). The incorporation of single and mixed starters during fermentation resulted in consistent microbiological and chemical characteristics, characterized by an increase in the overall count of LAB and TAT, accompanied by a decrease in pH. The incorporation of a mixed starter resulted in a higher overall concentration of LAB and TAT, as well as a more rapid decrease in pH, when compared with the use of a single starter. The chosen treatment method involved the incorporation of a mixed starter possessing certain characteristics, including a moisture content of 66.56%, ash content of 5.92%, protein content of 23.06%, fat content of 3.96%, and sodium chloride content of 2.57%. Both L. plantarum SK(5) and P. pentosaceus BP(20) were deemed suitable for use as starters in bekasam fermentation
Peningkatan Pengetahuan dan Keterampilan Warga Desa Babakan melalui Pelatihan Pembuatan Cookies Spirulina Iriani Setyaningsih; Kustiariyah Tarman; Safrina Dyah Hardiningtyas; Rizfi Faris Pari; Desniar; Uju Uju; Wahyu Ramadhan; Tati Nurhayati
Agrokreatif: Jurnal Ilmiah Pengabdian kepada Masyarakat Vol. 10 No. 1 (2024): Agrokreatif Jurnal Ilmiah Pengabdian Kepada Masyarakat
Publisher : Institut Pertanian Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/agrokreatif.10.1.76-84

Abstract

Cookies are dry cakes made with flour as the primary baking ingredient. Spirulina platensis was rich in protein, amino acids, glycoproteins, essential fatty acids, polysaccharides, and beta-carotene. Therefore, using sago flour combined with spirulina and seaweed in cookies can increase the nutritional value and produce gluten-free cakes. This training aims to increase knowledge about spirulina, packaging, and product marketing strategies and the skills of Babakan village residents by providing training on making spirulina cookies. This community service activity was conducted by counseling and training on making spirulina cookies in Babakan Village, Dramaga. Counseling activities were carried out to increase knowledge about the potential of spirulina, good packaging, and product marketing strategies, as well as training on making spirulina cookies to improve the skills of Babakan village residents. There were 18 training participants: Family Welfare Empowerment administrators and members. Pre-tests and post-tests were carried out before and after the training activities regarding the topic provided by the speakers. The achievements of the training activities were seen from an increase in post-test results, which was 60% answered correctly compared to only 40% for the pre-test. The evaluation results of the participant's acceptance of training topics and training speakers had an average of 58 to 69%, respectively, perfect. While the participants's assessment of the training facilities and consumption had an average of 50%, they were good.
Purification of Phycocyanin from Spirulina platensis Using Natural Deep Eutectic Solvents with Varied Hydrogen Bond Donor Eirene Tentua; Safrina Dyah Hardiningtyas; Iriani Setyaningsih
Jurnal Ilmiah Perikanan dan Kelautan Vol. 17 No. 1 (2025): JURNAL ILMIAH PERIKANAN DAN KELAUTAN
Publisher : Faculty of Fisheries and Marine Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jipk.vi.54788

Abstract

Graphical Abstract  Highlight Research NaDES-based purification offers a sustainable and cost-effective method for extracting phycocyanin from Spirulina platensis. ChCl:Citric Acid (NaDES) achieved 2.3-fold purity (71.83%), surpassing ammonium sulfate precipitation (70.15%). Phycocyanin retained its structural integrity, with enhanced antioxidant activity (IC50 = 40.54 ppm) post-purification. The aqueous two-phase system (ATPs) efficiently separated impurities, improving extraction scalability. This method supports applications in nutraceuticals, cosmetics, and functional food industries as a green alternative.     Abstract Phycocyanin, a pharmacologically potent blue pigment extracted from Spirulina platensis, requires optimal purity for effective utilization. Traditional purification methods, although effective, are impeded by time and cost constraints. Addressing this challenge, aqueous two-phase systems (ATPs) incorporating natural deep eutectic solvents (NaDES) emerge as promising alternatives. These systems offer operational simplicity and cost-effectiveness, ensuring efficient purification with high purity and optimal recovery of phycocyanin. This study investigated the impact of various types of hydrogen bond donors (HBD) within choline chloride-based natural deep eutectic solvents (NaDES) on the purification of phycocyanin. Phycocyanin was extracted from Spirulina platensis biomass through ultrasonication and purified using an aqueous two-phase system with various HBD of NaDES, including urea, glycerol, and citric acid. The experimental design adhered to a completely randomized design. The initial purity index and yield of crude extract phycocyanin were 0.54±0.02 and 12.79±0.27%, respectively. The NaDES system with ChCl:citric acid exhibited superior performance, demonstrating a high purity index and recovery (2.3-fold, 71.83±2.36%) compared to ammonium sulfate (1.5-fold, 70.15±4.10%). The obtained phycocyanin was partially pure compared to commercial phycocyanin (purity index: 1.60), as indicated by SDS-PAGE. Moreover, the antioxidant activity of phycocyanin was enhanced post-purification, evident in the IC50 value of 40.54 ppm. In summary, organic acid-based NaDES has proven effective in increasing the purity and achieving a significant recovery percentage of phycocyanin compared to conventional ammonium sulfate methods. The antioxidant activity of phycocyanin was enhanced after purification. These results indicate the promising potential of NaDES-based ATP systems for producing functional protein-based ingredients, exemplified by phycocyanin.