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Iriani Setyaningsih
Departemen Teknologi Hasil Perairan, Fakultas Perikanan Dan Ilmu Kelautan Divisi Bioteknologi Kelautan, Pusat Kajian Sumberdaya Pesisir Dan Lautan Institut Pertanian Bogor
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The Influence of Harvesting Period on Lipid Associated Antioxidant Activity of Semicontinuously Grown Chlorella vulgaris Tjandara Chrismadha; Diani Sartika; Iriani Setyaningsih; Uju Uju
ANNALES BOGORIENSES Vol 14, No 1 (2010): Annales Bogorienses
Publisher : Research Center for Biotechnology - Indonesian Institute of Sciences (LIPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (522.466 KB) | DOI: 10.14203/ann.bogor.2010.v14.n1.25-30

Abstract

A green alga,  Chlorella vulgaris  was grown semi-continuosly at various harvesting periods, and the lipid content and its associated antioxidant activity was examined. The harvesting periods were 9, 18, and 27 days, while the algal culture was placed  in 10 L glass  bottles provided with aeration  for gas exchange and  mixing. Light  was  obtained  from  440  watt  cool  fluorescent  lamps  placed  at  one  side  of  the  culture.  Room temperature was  26-32C.  Algal  lipid  extraction  was  done  based  on  liquid  phase  separation  of  methanol :chloroform:water  and  the  antioxidant  activity  was  examined  by  means  of  oxidation  inhibitory  in ethanol emulsified  limnoleic  acid.  In  addition,  a  TLC  analysis  was  performed  to  identify  the  antioxidant compouns soluble  in  the  lipid.  The  results  showed  that  harvesting  per iod  had  a  significant  influence  on  the algal lipid  content,  which  were  11.94,  12.96,  and  16.51%  of  the  dry  weight  in  the  culture  with  harvesting periods of 9, 18, and 27 days, respectively. No remarkable effect of the  harvesting period on the  antioxidant activity, which were observed to inhibit oxidation of linoleic acid up to 67-71%. There were five compounds found can  be associated   with  the  algal  antioxidant  activity,  which  were  pheophorbide -a,  chlorophyll-b,chlorophyll-a, phaeophytin-a, -carotene, and an unidentified one. Key words: microalgae, Chlorella vulgaris, lipid, antioxidant, harvesting period 
Penilaian Risiko Semikuantitatif Logam Berat pada Ikan Salmon di Jabodetabek: Semi-Quantitative Risk Assessment of Heavy Metals in Salmon Distributed in Jabodetabek Nindya Hambar Wasisto; Wini Trilaksani; Iriani Setyaningsih
Jurnal Pengolahan Hasil Perikanan Indonesia Vol 25 No 2 (2022): Jurnal Pengolahan Hasil Perikanan Indonesia 25(2)
Publisher : Masyarakat Pengolahan Hasil Perikanan Indonesia (MPHPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17844/jphpi.v25i2.40550

Abstract

Salmon imports continue to rise, while there were indications in some waters of the salmon's origin country that is a potential danger of heavy metals contamination. This phenomenon raises awareness regarding the food safety of salmon consumption, therefore a risk assessment is crucial to be conducted. The purposes of this study were to determine the content of Hg, Pb, and Cd in salmon meat and to assess the risk of heavy metals in salmon consumption in Jabodetabek area. Salmon fish, 132 in total was randomly taken from Tanjung Priok Port, the heavy metal analysis used the Flameless Inductively Coupled Plasma Mass Spectrometry instrument, risk assessment based on the United States Environmental Protection Agency method and risk ranger based on Ross and Sumner, supported by systematic literature review and questionnaires. The heavy metals Hg, Pb, and Cd in salmon were detected at 0.0646±0.0056, 0.0505±0.0446, and 0.0119±0.0006 ppm, respectively, indicating that there were still below the thresholds set by Food and Drug Supervisory Agency of the Republic of Indonesia (0.5;0.2;and 0.1 ppm). The results of the semi-quantitative risk assessment for Hg, Pb, and Cd were 0.0567, 0.0013, and 0.0010 (less than 1) implying that salmon consumption have no a potential risk of hazard. However heavy metals are accumulated in human body, hence, the diet consumption analysis needed to be performed. The risk ranger showed that the estimated risk ranking of heavy metals was in the medium category. Based on the results, it can be concluded that imported and consumed salmon by Jabodetabek residents was still relatively safe.
Fraksinasi Flavonoid Spirulina platensis dengan Metode Kromatografi Lapis Tipis dan Aktivitas Inhibisi Enzim α-Glukosidase Hartoyo Notonegoro; Heder Djamaludin; Iriani Setyaningsih; Kustiariyah Tarman
Jurnal Kelautan Tropis Vol 25, No 3 (2022): JURNAL KELAUTAN TROPIS
Publisher : Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jkt.v25i3.13905

Abstract

Spirulina platensis is a type of Cyanobacterium microalgae that forms multicellular helicoidal filaments. Spirulina platensis contains primary and secondary metabolites. The type and amount of the active compound Spirulina platensis depends on the method of extraction, fractionation, and isolation. So far, there are not much research data related to the active compound of Spirulina platensis extract cultured on Walne. The purpose of this study was to use of thin layer chromatography (TLC) method to separate flavonoids from Spirulina platensis biomass extract cultured on Walne media and the activity of a-glucosidase enzyme inhibition using biomass, crude extract, active fraction of flavonoids and phycocyanins from Spirulina platensis. This research method is descriptive experimental, which the Spirulina platensis is cultured on 80 g/L NaNO3 modified Walne media, extracted by maceration, fractionated by TLC and isolated the active compound by Preparative TLC (PTLC). The analysis included fraction and isolation of flavonoids from Spirulina platensis. The results showed that the TLC could be used to identify the active compound of Spirulina platensis extract cultured on Walne. Fractionation of Spirulina platensis extract using stationary phase silica gel Si 60 GF254 and the best mobile phase with a combination of chloroform:ethyl acetate (6:4) and an Rf1 value of 0.58; Rf2 0.71; and Rf3 0.83, as well as yellow-orange spots. Isolation of the active compound of Spirulina platensis extract using PLTC stationary phase silica gel Si 60 PF254 and the best mobile phase combination eluent chloroform:ethyl acetate (9:1). RF value of Rf2 0.57; Rf3 0.86; and Rf4 0.93 with dark yellow-brown spots. The color of the spots from the PLTC results shows that the active compounds of Spirulina platensis extract are flavonoid compounds. Biomass, crude extract, phycocyanine extract and flavonoids from Spirulina do not have inhibitory activity against α-glucosidase enzyme.  Spirulina platensis merupakan jenis mikroalga Cyanobacterium yang membentuk filamen helicoidal multiseluler. Spirulina platensis mengandung senyawa metabolit primer dan sekunder. Jenis dan jumlah senyawa aktif Spirulina platensis tergantung pada metode ekstraksi, fraksinasi dan isolasi. Sejauh ini belum banyak data hasil penelitian terkait senyawa aktif ekstrak biomassa Spirulina platensis yang dikultur pada media Walne. Tujuan penelitian ini yaitu penggunaan metode kromatografi lapis tipis (KLT) untuk memisahkan flavonoid ekstrak biomassa Spirulina platensis yang dikultur pada media Walne serta aktivitas inhibisi enzim α-glukosidase menggunakan biomassa, ekstrak kasar, fraksi aktif flavonoid dan fikosianin dari Spirulina platensis. Metode penelitian ini adalah eksprimental deksriptif dimana mikroalga Spirulina platensis dikultur pada media Walne modifikasi 80 g/L NaNO3, diekstraksi dengan maserasi, difraksinasi dengan KLT dan diisolasi senyawa aktif dengan KLT Preparatif (KLTP). Analisis yang dilakukan meliputi analisis fraksi dan isolasi flavonoid dari Spirulina platensis. Hasil penelitian menunjukkan metode KLT dapat digunakan untuk mengindetifikasi senyawa aktif ekstrak Spirulina platensis yang dikultur pada media Walne. Fraksinasi ekstrak Spirulina platensis menggunakan fase diam silika gel Si 60 GF254 dan fase gerak kombinasi eluen terbaik kloroform:etil asetat (6:4) dan nilai Rf1 0,58; Rf2 0,71; dan Rf3 0,83, serta bercak berwarna kuning-oranye. Isolasi senyawa aktif ekstrak Spirulina platensis menggunakan KLTP fase diam silika gel Si 60 PF254 dan fase gerak kombinasi eluen terbaik kloroform:etil asetat (9:1). Nilai Rf2 0,57; Rf3 0,86; dan Rf4 0,93 dengan bercak berwarna kuning-cokelat gelap. Warna bercak hasil KLTP menunjukkan komponen senyawa aktif ekstrak Spirulina platensis berupa senyawa golongan flavonoid. Biomassa, ekstrak kasar, ekstrak fikosianin dan flavonoid dari Spirulina tidak memiliki aktivitas penghambatan terhadap enzim α-glukosidase.
In Vitro and In Vivo Malondialdehyde Inhibition Activities of Stichopus hermanii and Spirulina platensis Mega Safithri; Kustiariyah Tarman; Iriani Setyaningsih; Yanti Fajarwati; Imanniar Yuta Ellana Dittama
HAYATI Journal of Biosciences Vol. 29 No. 6 (2022): November 2022
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.29.6.771-781

Abstract

Previous research showed that Stichopus hermannii and Spirulina platensis had an antioxidant activity. It is indicated by the reduced malondialdehyde (MDA) level in the liver of the diabetic rats. However, the STZ administration did not significantly increase MDA concentration of diabetic rats' blood serum for 14 days. This research aimed to determine in vitro and in vivo MDA inhibition of S. hermanii and S. platensis. The in vitro antioxidant activity test was conducted using the MDA-TBA method, and a positive control used α-tocopherol. For in vivo experiment, diabetic rats (DM) were induced by streptozotocin for 21 days. Twenty-five rats which were divided into five groups: normal rat group (NA), diabetic rat group (DA), diabetic rat group + glibenclamide (DG), diabetic rats + Stichopus hermanii (SH), and diabetic rats + Spirulina platensis (SP). The in vitro results showed that the antioxidant activity of 25 ppm Spirulina platensis had the same MDA inhibitory activity as 200 ppm α-tocopherol, but 200 ppm Stichopus hermanii had lower inhibition than 200 ppm α-tocopherol. The in vivo result showed that Stichopus hermanii treatment was more effective in suppressing blood serum MDA concentration, but Spirulina platensis was more effective in suppressing liver MDA concentration.
Co-Authors . Uju Aksar Chair Lages Alwahidul Mubarok Anisa Gianti Zhafira Apon Zaenal Mustopa Bustami Ibrahim Dedi Noviendri Delicia Delicia Desniar - - Diani Sartika Dwi Susilaningsih Eirene Tentua Endar Marraskuranto Evi Purnamasari Hana Nurullita Prestisia Hartoyo Notonegoro Hartoyo Notonegoro Hasanah Hasanah Heder Djamaludin Huriyah, Siti Balqis Imanniar Yuta Ellana Dittama Jae-Kwan Hwang Julian Franklin Soselisa Kustiariyah Tarman Mawaddah Renhoran Mega Safithri Meydia Meydia Meydia Meydia Mirza Gulam Ahmad Muhamad Ridwan Muhammad Nursid Nabila Ukhty Nindya Hambar Wasisto Nurul Mutia Putram Patmawati Patmawati PUTRIANA SARI SIRAIT Retno Santi Sumardi Rifqiyah Nur Umami safrina dyah hardiningtyas Sari Afriani Sugeng Heri Suseno Swestri Utami Tjandara Chrismadha Uju Uju Untung Sudadi Vitriyanna Mutiara Yuhendri Wini - Trilaksani Yanti Fajarwati Yogiara Yogiara