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Potency of Catfish (Clarias sp.) Protein Hydrolysates as Candidates Matrices for Microbiology Reference Material Kurniawati, Eti; Ibrahim, Bustami; Desniar, Desniar
Squalen, Buletin Pascapanen dan Bioteknologi Kelautan dan Perikanan Vol 14, No 3 (2019): December 2019
Publisher : Research and Development Center for Marine and Fisheries Product Processing and Biotechnol

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (579.542 KB) | DOI: 10.15578/squalen.v14i3.404

Abstract

Fish protein hydrolysate (FPH) is a derivative product of fish proteins containing smaller peptides and amino acids. FPH products have high water solubility, good emulsion capacity, and large expanding ability. With its functional properties, it allows FPH to be used as a raw material in the manufacturing of secondary microbiological reference materials. This study was intended to characterize catfish (Clarias sp.) FPH as a candidate for the matrix of microbial secondary reference. The FPH was prepared through enzymatic hydrolysis, freeze-drying and milling. The hydrolysis processes were carried out using 5% (w/w) papain, 55 °C for 5 hours, then the papain activity was stopped by increasing the temperature to 80 °C for 20 minutes.The FPH was combined with gelatine, sodium glutamate, glucose solution, and was spiked with Salmonella enteritica sv Enteritidis and freeze-dried. Results showed that catfish FPH was yellowish-white powder with a FPH yield of 11.05%. The proximate analysis of FPH revealed the moisture content of 3.77 ± 0.12%, ash content of 7.26 ± 0.03%, protein content of 86.09 ± 0.17%, and fat content of 1.38 ± 0.07%. The protein content of the FPH was greater than skim milk (33.42%). Carbohydrate levels of catfish FPH and skim milk were 1.56% and 57.46%, respectively. The best concentration of catfish FPH to perform as a microbiological reference material was 14%, obtained from highest viability of Salmonella bacteria and homogeny. The candidate for reference material were stable at storage temperatures of -20 oC.
KARAKTERISASI MINYAK IKAN DARI HASIL SAMPING INDUSTRI PENEPUNGAN IKAN LEMURU (Sardinella lemuru) DENGAN METODE PEMURNIAN ALKALI Ibrahim, Bustami; Suptijah, Pipih; Yogaswara, Ghema
Dinamika Maritim Vol 5 No 1 (2015): Dinamika Maritim, Vol. 5 No. 1, April, 2015
Publisher : Pusat Penelitian Sumberdaya Pesisir dan Laut, Universitas Maritim Raja Ali Haji, Indonesia

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Abstract

Utilization of fish Lemuru ( Sardinella lemuru ) into fish meal has a byproduct in the form of fish oil . It is known that the content of omega-3 fatty acids in fish oil lemuru highly enough . So that the existing oil content in fish processing waste of lemuru needs to be recovered by purification , so that fish oil obtained has better quality . In this study, fish oil is purified by the alcaline refining methode . The result of the process of purifying fish oils have a better quality than that of unrefined fish oil . But the content of saturated fatty acids to decrease. The quality of fish oil purified with alcaline methode and purification done by the plant were not significantly different
Optimasi dan Pemodelan Proses Recover Flavor Dari Limbah Cair Industri Pengolahan Rajungan Dengan Reverse Osmosis . Uju; Bustami Ibrahim; Wini Trilaksani; Tati Nurhayati
Jurnal Ilmu Pertanian Indonesia Vol. 14 No. 1 (2009): Jurnal Ilmu Pertanian Indonesia
Publisher : Institut Pertanian Bogor

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Abstract

The waste water of blue crab pasteurization has potential in environmental pollution. It contained TSS of 206.5mg.1-1, BOD 7,092.6mg.1-1 and COD of 51,000mg.1-1. on the other hand, it also contains an interesting flavor compound, which composed of 0.23% non protein nitrogen and 17 amino acids where the highest was glutamic acid one. In this study, pre-filtration step using filter size 0.3 µ followed by reverse osmosis has been used to reduce these pollutions load and flavor compound recovery. During pre-filtration steps, TSS was reduced to 74.8% so turbidity decrased reased until 31%. After reverse osmosis process, BOD, and COD decreased more than 99%, and there was no amino acids detected in permeate stream. Factors that affect performance of reverse osmosis were transmembrane pressure, temperature and pH. The higher transmembrane pressure, temperature and pH resulted the higher the flux permeate. The use of higher temperature make flux increasing, eventually increasing transmembrane pressure make the flux increased only at transmembrane pressure less than 716 kPa. The protein rejection was influenced unsignifanctly by transmembrane pressure, temperature and pH. During concentrating flux declined exponentially by time function. At concentration factor 2.75 resulted 79% and 12% of increasing protein and NPN, respectively. The amino acids content can be increased 2−23 times of the origin. Even arginin and sistin, the amino acids that were undetectable initially, but they can bedetected at concentration of 0.0360 and 0.0250 (w/v) respectively at the end of the process. Hidrolysis and fermentation process can increase the amino acid content 31−45 times.
KINERJA MICROBIAL FUEL CELLS PADA PENGOLAHAN LIMBAH CAIR PEMINDANGAN DENGAN MEMBRAN SEPARATOR CAMPURAN POLIMER KITOSAN/PVA Bustami Ibrahim, Pipih Suptijah, dan Fajar Syahreza
Jurnal Teknologi Industri Pertanian Vol. 27 No. 3 (2017): Jurnal Teknologi industri Pertanian
Publisher : Department of Agroindustrial Technology, Bogor Agricultural University

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Abstract

Separator attched to MFC system can reduce the distance between cathode and anode and limit oxygen penetration to anode chamber. The use of Chitosan/polyvinyl alcohol (PVA) mixture polymer as membrane separator was expected to have ability to increase electron recovery from the substrate. The objectives of this research were to increase the standard quality of wastewater of salted boiled fish processing and to increase bioelectricity generation, through the membrane separator adding to MFC system. Separator polymer was made by blending 2% chitosan and 5% PVA solution, in different ratio of chitosan (K) and PVA (P) at 2:3 (K2P3), 1:1 (K1P1), 3:2 (K3P2), and without separator. The study was carried out to measure electricity generated and to analyse of BOD, COD and TAN of the wastewater.  The K2P3 treatment showed the highest electricity generation with average voltage of 0.50±0.06 V, maximum current density 0,29 mA/cm2, and  maximum power density  15.40 mW/cm2. The reduction of COD showed insignificantly different among treatments, but the reduction of BOD and TAN values showed significantly different. The BOD value reduced to56.54% in K2P3 treatment and the TAN value reduced to 92.73% in K1P1 treatment.Keywords : chitosan, microbial fuel cell, polyvinil alcohol, separator
PROSES RECOVERY DAN PENGKONSENTRASIAN PROTEIN AIR PEREBUSAN PINDANG TONGKOL (Euthynnus affinis) MELALUI ULTRAFILTRASI Uju, Bambang Riyanto, Bustami Ibrahim, Wahyu Ramadhan, Irfan Setia Tanjung
Jurnal Teknologi Industri Pertanian Vol. 27 No. 3 (2017): Jurnal Teknologi industri Pertanian
Publisher : Department of Agroindustrial Technology, Bogor Agricultural University

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Abstract

“Pindang” is a  fisheries product which is produced by combination of boiling and salting. Boiling in a salt solution causes dissolving protein into the boiling water. Membrane process has been applied for the concentrating of protein. In this study, the characterization and recovery of protein from the boiled water of “pindang” were carried out using 0.05 µm ultrafiltration membrane. The parameters of study included the effect of the concentration factor on the protein concentration, flux of permeate, and protein rejection coefficient. The concentrating of “pindang” boiling water using 0.05 μm ultrafiltration membrane increased 46.75% of protein. During ultrafiltration concentrating process, flux decreased from 6.58 L/m2h at the beginning of the concentration to 2.71 L/m2h at fortieth minute. Rejection coefficient increased from 41.2% at the beginning of the concentrating to 55.07% at fortieth minute. The decreasing values of the parameters COD, TSS, turbidity, protein, and fat were higher than the decreasing of TDS, salinity, and pH. The dominant molecular weight of protein in protein concentrate of “pindang” boiling water was 33.45 kDa.Keywords: protein concentrate, “pindang”, ultrafiltration
Characterization and Recovery of Minced fish Wastewater Protein using Reverse Osmosis . Uju; Tati Nurhayati; Bustami Ibrahim; Wini Trilaksani; Maglory Siburian
Jurnal Pengolahan Hasil Perikanan Indonesia Vol 12 No 2 (2009): Jurnal Pengolahan Hasil Perikanan Indonesia
Publisher : Department of Aquatic Product Technology IPB University in collaboration with Masyarakat Pengolahan Hasil Perikanan Indonesia (MPHPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (175.573 KB) | DOI: 10.17844/jphpi.v12i2.855

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Recovery of minced fish wastewater soluble protein was done by reverse osmosis membrane. Themincefish wastewater has high level polluting load. It contains COD and TSS 4.233 and 55,06 mg/lrespectively, but it is potential to utilized because of high protein and amino acids content especiallyglutamic acid. Fluxes of permeate during recovery process were kept on steady state in the beginningprocess at 15 l/m2h without prefiltration and 20 l/m2h with 0,9 micron prefiltration. During concentration,flux time decreased from 22,11 l/m2h at concentration factor 1 to 12,95 l/m2h at concentration factor 1,52.In other sides the rejection value of protein increased from 90,11% in the beginning process to 99,50% inthe end of the concentration process. At the end of the recovery stage, yield concentrate contained 0.401 g/lwhich is 21 fold greater than the original step. Therefore permeate stream delivered high quality effluentand met the standard of wastewater effluent.Keywords: minced fish, protein, recovery, reverse osmosis
The Utilization of Chitosan on Fishery Industrial Wastewater Treatment Bustami Ibrahim; Pipih Suptijah; . Prantommy
Jurnal Pengolahan Hasil Perikanan Indonesia Vol 12 No 2 (2009): Jurnal Pengolahan Hasil Perikanan Indonesia
Publisher : Department of Aquatic Product Technology IPB University in collaboration with Masyarakat Pengolahan Hasil Perikanan Indonesia (MPHPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (194.28 KB) | DOI: 10.17844/jphpi.v12i2.879

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Highly organic substances contained within fishery industrial wastewater will pollute the environment when it flows into public water body without any previous treatment. Chitosan is polymersubstance extracted from shrimp shell which is able to wrap solid particles suspended in liquids and bring them together and agglomerate makes it suitable as a coagulant aid. The objective of this research was to study the effectiveness of chitosan in order to decrease pollutant parameter in the fishery industrial wastewater. The quality of chitosan used during this research confirmed to Protan Laboratory standard. Results showed that chitosan as a coagulant has good performance in pH range of 6.6-6.8 and at concentration of 175-225 ppm. Within these conditions, chitosan could decrease turbidity value 94.33%-95.17%, TSS (Total Suspended Solids) 95.10%-95.82%, BOD (Biochemical Oxygen Demand) 72.09%-79%, COD (Chemical Oxygen Demand) 76.72%-79.40%, TKN (Total Kjeldahl Nitrogen) 12.50%-27.82%, NH3 80.84%-82.56%, NO2-N 47.69%-54.74%, NO3-N 19.39%-40.14%. The best chitosan concentration to use was at 200 ppm. The quality of effluent produced confirmed to Standard Regulation Indonesian Ministry of Environmental for Standard of Industrial Wastewater no.:KEP-51/MENLH/10/1995.Keywords: chitosan, coagulant, fishery industrial wastewater treatment
Biokinetic Parameter Values of Denitrification Process of Fishery Industrial Wastewater on Differentiated COD/TKN Ratios Bustami Ibrahim; Anna C. Erungan; . Heriyanto
Jurnal Pengolahan Hasil Perikanan Indonesia Vol 12 No 1 (2009): Jurnal Pengolahan Hasil Perikanan Indonesia
Publisher : Department of Aquatic Product Technology IPB University in collaboration with Masyarakat Pengolahan Hasil Perikanan Indonesia (MPHPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (140.012 KB) | DOI: 10.17844/jphpi.v12i1.890

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Indonesian Fishery Industries has been developed very rapidly. This can cause negative effect such as increase in wastewater that involved highly organic substrates that will danger the environment. This effluent must be treated before flowed it to environment and usually treated by biological wastewater treatment through nitrification and denitrification process simultaneously using activated sludge. This study conducted denitrification process using activated sludge with treatment using different COD/TKN ratios which are 2.44, 2.96, and 3.26 and monitored in Hydraulic Retention Time (HRT) 4.5, 3.5, 2.5, 2.0, 1.5, 1.0, 0.75, 0.5 and 0.25 day. Result showed that for determining the biokinetic parameters such as Ks, Kno (Monod Constant saturated), Y (yield), kd (endogenous decay) also μm result for range of COD/TKN ratios 2.44–3.26 are 3.04 are 14.93–17.26 mg/lCOD, 1.21-1.60 mg/l NO3, 0.171-0.193 mg VSS/mg COD, 0.014-0.062 day-1 and 1.64–0.97 day-1. These biokinetic parameters can be used to improve the wastewater treatment plant.Keywords: activated sludge, biokinetic parameter, denitrification
Penggunaan Bentonit dalam Pembuatan Sabun dari Limbah Netralisasi Minyak Ikan Lemuru (Sardinella sp) Bustami Ibrahim; Pipih Suptijah; Slamet Hermanto
Jurnal Pengolahan Hasil Perikanan Indonesia Vol 8 No 2 (2005): Buletin Teknologi Hasil Perikanan
Publisher : Department of Aquatic Product Technology IPB University in collaboration with Masyarakat Pengolahan Hasil Perikanan Indonesia (MPHPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (327.827 KB) | DOI: 10.17844/jphpi.v8i2.1009

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Proses pemurnian minyak ikan lemuru menghasilkan limbah yang memiliki warna dan bau yang merusak lingkungan. Limbah ini merupakan hasil penyabunan dari asam lemak bebas dari minyak ikan. Tujuan dilakukannya penelitian ini adalah untuk mengetahui kemampuan bentonit sebagai adsorben dalam menghilangkan bau dan warna dari limbah netralisasi minyak ikan lemuru (Sardinella sp) agar sabun yang dihasilkan dapat bermanfaat. Penelitian pendahuluan yang telah dilakukan menunjukkan bahwa pengolahan dengan perbandingan antara limbah minyak ikan lemuru, NaOH dan NaCl 15 % (b/v) yaitu 1:3:0,1 adalah yang terbaik berdasarkan uji kesukaan terhadap warna sabun minyak ikan lemuru, sehingga dijadikan kontrol pada penelitian utama. Hasil analisis kimia produk sabun pada penelitian utama dengan konsentrasi bentonit yang berbeda-beda menunjukkan bahwa kadar air sabun minyak ikan lemuru berkisar antara 58,32-64,54 %. Nilai pH sabun berkisar antara 10,96-12,01. Kadar asam lemak bebas sabun minyak ikan lemuru tidak terdeteksi dan kadar alkali bebas berkisar antara 0,10-0,15 %. Kadar total nitrogen pada sabun minyak ikan lemuru berkisar antara 0,45-0,56 %. Kadar amonia sabun minyak ikan lemuru untuk semua perlakuan tidak terdeteksi. Uji hedonik menunjukkan bahwa sabun minyak ikan lemuru dengan penambahan bentonit yang berbeda tidak memberikan perbedaan yang nyata terhadap kesukaan panelis terhadap warna, bau maupun tekstur.Kata kunci: bentonit, limbah netralisasi minyak, minyak ikan lemuru, sabun
Kaji Ulang Sistem Pengolahan Limbah Cair Industri Hasil Perikanan secara Biologis dengan Lumpur Aktif Bustami Ibrahim
Jurnal Pengolahan Hasil Perikanan Indonesia Vol 8 No 1 (2005): Buletin Teknologi Hasil Perikanan
Publisher : Department of Aquatic Product Technology IPB University in collaboration with Masyarakat Pengolahan Hasil Perikanan Indonesia (MPHPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (53.2 KB) | DOI: 10.17844/jphpi.v8i1.1028

Abstract

Industri pengolahan hasil perikanan mengkonsumsi air mencapai 20m3/ton produk yang dihasilkan tergantung pada teknologi yang digunakan, jenis ikan yang diproses dan produk yang dihasilkan. Limbah cair yang dihasilkannya mengandung bahan organik yang tinggi dengan beban mencapai 20 kg BOD/ ton. Beban limbah yang tertinggi berasal dari industri pengalengan dan pembuatan tepung ikan (fishmeal). Limbah yang baru diolah bertujuan untuk menyisihkan beban organik, belum mencapai penyisihan total nitrogen yang terkandung didalamnya. Kandungan nitrit dan nitrat yang masih tinggi akan menurunkan kualitas badan air penerima. Teknologi pengolahan limbah cair perikanan dengan kombinasi proses aerobik dan anoksik menjadi pilihan yang baik dikembangkan untuk penyisihan nitrogen yang ada di dalam limbah.Keyword: Industri perikanan, limbah cair, lumpur aktif.
Co-Authors . Heriyanto . Prantommy . Uju . Uju Ade Komalasari Suhendar Adenia Cahyatie Aprillia Agus Muhamad Soleh Ahmad Zahid Aldo Reshwara Alvi Nur Yudistira Alvindo Chrisna Mukti Anna C. Erungan As Syaffa Amalia Adha Asadatun Abdullah Aulia Andhikawati Bagus Sukma Agung Dadan Heryada Wigenaputra Dadi Rochnadi Sukarsa Desi Listiana Desniar - - Dewi Mutamimah Dwi Winarsih Ella - Salamah ETI KURNIAWATI Farah Nurjannah Farell Pramuja Fariz Pari, Rizfi Giri Rohmad Barokah Henggu, Krisman Umbu Hijrah Amin Hirda Umayyah I Dewa Made Subrata Iffani Nabila Zain Irama Dramawanti Pamungkas Iriani Setyaningsih Irma Irma Jannah, Arum Jayanti, Feby Dwi Joko Santoso Kurniawan, Farhan Kustiariyah Tarman Laode Muhamad Hazairin Nadia Linda Aryanti Maglory Siburian Mandela, Risco Mohammad Haryst Daryaly Mudji Ana Yanti Muhamad Reza Fahlepi Muhammad Zaki Dama Nazihah, Nilna Tsabita NONI DWI SARI Nur Shasqia Malewa Nurhaliza, Ghefira Nurjanah Nurmaida Nurmaida Nurul - Hak Patmawati Patmawati Pipih Suptijah Pramesti, Nabilla Adhiany Pratiwi, Widya Prapti Purwadi, Wiwik Raffiq, Fathurrizqi Ramadhan, Rahmat Agung Ravellino Fahlepi Reni Lobo Ridho Orilda Rizki Tri Kurnia Ramadhan Roni Nugraha Rudy Nistibaskara safrina dyah hardiningtyas Safrina Dyah Hardiningtyas Setyarini, Dian Shabrina Itsnaini Oktafira Sidik, Adi Permana Sita Permata Sari Slamet Hermanto Steffen Sugeng Heri Suseno Surya Herlambang, Firhan Swastikawati, Ayu Setiti syeila - rosmalawati TATI NURHAYATI Theresia Puspita Arumsari Uju Uju Uju Uju Uju Uju Untung Sudadi Wini - Trilaksani Yogaswara, Ghema Yulia Oktavia YULYA FITRIA Zhalindri Noor Adjani