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All Journal Jurnal Pengolahan Hasil Perikanan Indonesia Forum Pasca Sarjana JOURNAL OF COASTAL DEVELOPMENT Squalen Bulletin of Marine and Fisheries Postharvest and Biotechnology
Ekowati Chasanah
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Education Environmental Science Immunology & microbiology

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Fatty Acid Profile, Carotenoid Content, and In Vitro Anticancer Activity of Karimunjawa and Lampung Sea Cucumber Ekowati Chasanah; Yusro Nuri Fawzya; Kustiariyah Tarman; Hedi Indra Januar; Muhammad Nursid
Squalen, Buletin Pascapanen dan Bioteknologi Kelautan dan Perikanan Vol 11, No 3 (2016): December 2016
Publisher : Research and Development Center for Marine and Fisheries Product Processing and Biotechnol

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/squalen.v11i3.269

Abstract

Fatty acids and carotenoid has been known as an anticancer agent on both preventing and treating cancer disease. This study was conducted to analyze the fatty acid profile, carotenoid and in vitro anticancer activity of 12 sea cucumber harvested from Karimunjawa and Lampung waters. The aim of the study was to determin the potency of sea cucumbers as raw material for nutraceutical products. Fatty acid profile and carotenoid content were characterized by gas chromatography and spectrophotometry techniques, while in vitro anticancer activity was assessed by MTT assay against cervix (HeLa), breast (T47D and MCF-7) and colon (WiDR) cancer cells. Results of the study showed polyunsaturated fatty acid (PUFA) dominated the composition of fatty acids in the samples from both locations. Holothuria sp. was detected to contain the highest amount of carotenoid. Furthermore, the highest in vitro anticancer activity was detected also in the sample of Holothuria sp. The activity of 30 ppm Holothuria sp. extract against HeLa cell was detected to be almost equal to the 5 ppm doxorubicin control. Concentration of 5 ppm Holothuria sp. extract also showed positive result in killing 50% of MCF-7 and T47D, but capable to 100% kill HeLa and WiDR cells. At concentration of 25 ppm, the extract was able to kill all the 4 cells tested. Statistical analysis showed the amount of carotenoid and two particular fatty acid compounds (docosadienoic and eicosapentaenoic acid) significantly (P0.05) contributed to the cytotoxic activity that was found in the sea cucumber samples. Those compounds were found in highest concentration from Holothuria sp harvested from Lampung waters, thus being the most prospective raw material for nutraceutical or functional food ingredient with anticancer potency.
BACTERIAL DIVERSITY OF THE DEEP SEA OF SANGIHE TALAUD, SULAWESI Gintung Patantis; Ekowati Chasanah; Dewi Seswita Zilda; Ikhsan B. Waluyo
Squalen, Buletin Pascapanen dan Bioteknologi Kelautan dan Perikanan Vol 7, No 1 (2012): May 2012
Publisher : Research and Development Center for Marine and Fisheries Product Processing and Biotechnol

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/squalen.v7i1.12

Abstract

Deep sea is an extreme environment characterized by cold temperature, high pressure, lackof  light and nutrients. Microorganisms live in these habitat are unique microorganisms andknown to have tremendous source of potential agents for biotechnology processes. Indonesia asan archipelagic country has a vast deep ocean. This study aims to see the diversity of bacteria inSangihe Talaud Deep Sea, Sulawesi. Analysis of bacterial diversity was carried out by culturedand uncultured method. Terminal Restriction Fragment Length Polymorphism (T-RFLP) techniquewas used for uncultured analysis of the microorganisms biodiversity, while cultured one wasdone by plating the samples of water onto Zobell media. The results showed that, there were 21isolates obtained by cultured method. The identification which based on 16S rDNA by PCR methodshowed the genus of Pseudomonas, Pseudoalteromonas, Alteromonas, Vibrio, Shewanella andUncultured bacterium were identified. However, 14 classes of bacteria were obtained by usingTRFLP method i.e Acetobacteraceae class, Actinobacteria, α-proteobacteria, -proteobacteria, δ-proteobacteria, γ-proteobacteria, Bacili, Bacteroidetes, Chlorobi, Chroococcales, Clostridia,Erysipelotrichi, Synergistia, and Zetaproteobacteria. here were also  unclassified bacteria anduncultured bacterium found in the samples.
BIOETHANOL PRODUCTION FROM SEAWEED PROCESSING WASTE BY SIMULTANEOUS SACCHARIFICATION AND FERMENTATION (SSF) Andi Hakim; Ekowati Chasanah; Uju Uju; Joko Santoso
Squalen, Buletin Pascapanen dan Bioteknologi Kelautan dan Perikanan Vol 12, No 2 (2017): August 2017
Publisher : Research and Development Center for Marine and Fisheries Product Processing and Biotechnol

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/squalen.v12i2.281

Abstract

Seaweed processing waste has been used for bioethanol production through simultaneous saccharification and fermentation (SSF). SSF is commonly used for bioethanol production to shorten the process and to increase the yield of ethanol produced by Trichoderma reesei and Saccharomyces cerevisiae. The aim of this research was to obtain the best concentration of T. reesei and S. cerevisiae to produce bioethanol by SSF. The concentration of T. reesei and S. cerevisiae used was 0 (control), 5, 10, 15 and 20% (v/v). The SSF process was carried out by using shaking incubator at 35 °C and rotation of 150 rpm for 3 days. The untreated and hot water treated seaweed processing waste used in this study have moisture content values of 12.94±0.08% and 15.38±0.19%, ash content values of 16.72±0.08% and 18.39±0.19%, lignin content values of 15.38±0.11% and 12.74±0.38%, and cellulose content values of 26.92±0.57% and 34.57±0.81%, respectively. The result of SSF process of seaweed processing waste showed that different concentrations of T. reesei and S. cerevisiae (control, 5, 10, 15 and 20%) yielded significant effect (p0.05) on the total reducing sugars and ethanol produced. The Duncan Multiple Range Test (DMRT) showed that the treatment 10% of T. reesei and S. cerevisiae concentration in the seaweed processing waste treated with hot water was the best treatment producing highest yield of ethanol.
SCREENING AND CHARACTERIZATION OF L-GLUTAMINASE PRODUCED BY BACTERIA ISOLATED FROM SANGIHE TALAUD SEA Ekowati Chasanah; Usman Sumo Friend Tambunan; Tanti Yulianti
Squalen, Buletin Pascapanen dan Bioteknologi Kelautan dan Perikanan Vol 7, No 3 (2012): December 2012
Publisher : Research and Development Center for Marine and Fisheries Product Processing and Biotechnol

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/squalen.v7i3.6

Abstract

L-glutaminase (L-glutamine amidohydrolase, EC 3.5.1.2) is a very important enzyme due to its role as flavor enhancer and antileukemic agent. Salt-tolerant L-glutaminase produced bymarine bacteria is favorable in food industries. This study describes the screening of L- glutaminase producing marine bacteria from Sangihe-Talaud Sea, North Sulawesi, Indonesia.Screening of L-glutaminase was performed using a liquid medium and identification of selected isolate was  performed using molecular-based 16S rDNA. Results showed that there were 7 isolates produced positive results of L-glutaminase, and one of them (II.1 isolate) produced the highest activity, i.e 147.99 U/L, equivalent to the specific activity of 62.32 U/mg. The isolate then selected for further study. Bacterial identification based on 16S rRNA sequencing has revealed that the isolate was 96% similar to Pseudomonas aeruginosa strain CG-T8. Characterization of extracellular L-glutaminase from the II.1 isolate showed that the enzyme worked optimally at temperature  of 37-45 °C and pH 7. The enzyme was stable when NaCl solution was added up to 8% and  began to decrease on addition of NaCl solution of 16% and 20% with relative activity of 79% and 74%, respectively. The effect of metal ions, e.g Mn2+, Mg2+, and Co2+ in the form of chloride salt, were able to increase enzyme activity, whereas the addition of other metal ions (Zn2+, Fe3+, and Ca2+) decreased the activity. The molecular weights of L-glutaminase was estimated around42 kDa and 145 kDa.
Co-Authors Agustinus R Uria Agustinus Robert Uria Andi Hakim Anthony D. Wright Catherine H. Liptrot Cherie A. Motti DAHRUL SYAH Dewi Seswita Zilda Dianne M. Tapiolas Gintung Patantis Hedi Indra Januar Hedi Indra Januar Ifah Munifah Ikhsan B. Waluyo Joko Santoso Kustiariyah Tarman M.R.R. Lukie Trianawati MAGGY T. SUHARTONO Mahrus Ali Muhammad Nursid Muhammad Nursid Nurrahmi Dewi Fajarningsih Tanti Yulianti TATI NURHAYATI Uju Uju Usman Sumo Friend Tambunan Yenny Risjani Yusro Nuri Fawzya Yusro Nuri Fawzya