Article Per Year (5 Year)
p-Index From 2020 - 2025
0.562
P-Index
This Author published in this journals
All Journal Science and Technology Indonesia Squalen Bulletin of Marine and Fisheries Postharvest and Biotechnology Jurnal Pascapanen dan Bioteknologi Kelautan dan Perikanan Teknologi dan Industri Pertanian Indonesia Molekul: Jurnal Ilmiah Kimia
Ifah Munifah
Unknown Affiliation
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Environmental Science Immunology & microbiology Industrial & Manufacturing Engineering Materials Science & Nanotechnology Physics

Published : 8 Documents Claim Missing Document
Claim Missing Document
Check
Articles

Found 8 Documents
Search

 1 
Produksi dan Karakterisasi Xilanase dari Isolat Bakteri M-13.2a Asal Air Laut Manado Fawzya, Yusro Nuri; Mangunwardoyo, Wibowo; Munifah, Ifah; Patantis, Gintung
Jurnal Pascapanen dan Bioteknologi Kelautan dan Perikanan Vol 8, No 1 (2013): Juni 2013
Publisher : Balai Besar Riset Pengolahan Produk dan Bioteknologi Kelautan dan Perikanan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/jpbkp.v8i1.53

Abstract

Isolat bakteri M-13.2A yang berasal dari laut Manado diketahui mampu menghasilkan enzim selulase dan xilanase, berdasarkan pembentukan zona bening pada media padat. Penelitian ini bertujuan untuk mendapatkan informasi lebih lanjut mengenai produksi dan sifat enzim xilanase yang dihasilkan dari isolat bakteri M-13.2A serta identifikasi isolat bakteri tersebut di atas. Sebanyak (2,4-3,3) x 108 cfu/ml inokulum dengan konsentrasi sekitar 9% (v/v) diinokulasikan dalam medium xylan broth, kemudian diinkubasi selama 6 hari pada suhu 30°C, 150 rpm. Pengambilan sampel dilakukan setiap hari dan enzim yang dihasilkan diuji aktivitasnya dengan metode asam dinitro salisilat (DNS). Hasil penelitian menunjukkan bahwa aktivitas xilanase tertinggi dihasilkan pada hari ke-2 inkubasi, sebesar 5,17 U/ml. Enzim xilanase ini bekerja optimum pada pH 8, suhu 70°C. Penambahan ion logam 10 mM memberikan pengaruh yang bervariasi terhadap aktivitas enzim. Ion Zn2+ meningkatkan aktivitas xilanase hingga 278,1%. Ion Fe3+ dan Ca2+ menurunkan aktivitas xilanase menjadi 75 dan 8,3% relatif terhadap kontrol, sedangkan ion K+ tidak memberikan pengaruh terhadap aktivitas xilanase. Hasil identifikasi bakteri menunjukkan bahwa isolat M-13.2A memiliki kemiripan 99% dengan Acinetobacter baumannii.
Antioxidant and Ace Inhibitor Potential of Stripe Trevally Fish (Selaroides leptolepis) Hydrolysate Reinal Putalan; Ifah Munifah; Tati Nurhayati; Ekowati Chasanah
Squalen, Buletin Pascapanen dan Bioteknologi Kelautan dan Perikanan Vol 13, No 1 (2018): May 2018
Publisher : Research and Development Center for Marine and Fisheries Product Processing and Biotechnol

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/squalen.v13i1.319

Abstract

This study aimed to investigate the potency of fish protein hydrolysates (FPH) of stripe trevally fish (Selaroides leptolepis) as antioxidant and ACE inhibitor. The FPH was produced through enzymatically hydrolysis using protease produced by Bacillus licheniformis, a collection of Research and Development Center for Marine and Fisheries Product Processing and Biotechnology (RDCMFPPB). The FPH was fractionated using ultrafiltration membranes with molecular weight cut off (MWCO) of 10, 5 and 3 kDa. The hydrolysis degree, protein content, peptide content, antioxidant activity and Angiotensin-converting enzyme (ACE) inhibitor were observed. The result showed that FPH had maximum hydrolysis degree of 63.91% reached after 6 hours hydrolysis with protein content of 27.43 mg/mL and peptide content of 223.32 mg/mL. That FPH showed antioxidant activity (IC50) of 1941.06 ppm and ACE inhibitor of 87.82% at test concentration of 10 mg/mL. In the ultrafiltration step, the higher molecular cut off used, the higher protein content and peptide content. The lower molecular weight of the hydrolysate, the better antioxidant  and ACE inhibitor activity. The best fractionation that produce potential product to be used as anti-hypertension was in mixture peptides between 3-5 kDa. The IC50 antioxidant activity was 1336.96 ppm and percentage of ACE inhibitor was 97.15% % (with a concentration of 10 mg/mL). From the results, protein hydrolysate of stripe trevally fish produced by enzymatic hydrolysis using local protease was potential as a functional ingredient, particularly as antihypertensive agent.
Astaxanthin: Senyawa Antioksidan Karoten Bersumber dari Biota Laut Ifah Munifah; Thamrin Wikanta
Squalen, Buletin Pascapanen dan Bioteknologi Kelautan dan Perikanan Vol 1, No 1 (2006): December 2006
Publisher : Research and Development Center for Marine and Fisheries Product Processing and Biotechnol

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/squalen.v1i1.71

Abstract

Meskipun berbagai jenis antioksidan dalam makanan telah berkontribusi dalam pencegahan penyakit, namun sejumlah riset yang telah dilakukan lebih memfokuskan pada tiga jenis antioksidan yakni vitamin E, vitamin C dan karotenoid karena berperan penting sebagai nutrien yang diperlukan dalam metabolisme tubuh manusia.
Evaluation of Antibacterial Assays for Screening of Marine Invertebrate Extracts Nurrahmi Dewi Fajarningsih; Ifah Munifah; Dewi Seswita Zilda
Squalen, Buletin Pascapanen dan Bioteknologi Kelautan dan Perikanan Vol 13, No 1 (2018): May 2018
Publisher : Research and Development Center for Marine and Fisheries Product Processing and Biotechnol

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/squalen.v13i1.294

Abstract

Marine environment continuously produces pharmacologically active compounds. To screen marine natural products as a source of potential antibiotics, it is very important to select an accurate and efficient antibacterial assay. This study aims to find out the best assay for screening of antibacterial activity of marine invertebrate extracts by comparing the performance of 3 methods, i.e. the colorimetric resazurin microtiter assay (REMA), disc diffusion assay and spectrophotometric microdilution assay (SMA). Five marine invertebrate extracts, i.e. Stylissa sp., Theonella sp., Lobophytum sp., Sarcophyton sp., and Aaptos sp., were tested using those 3 different methods. The best method obtained was then further tested for its performance to screen 126 marine invertebrate extracts against 2 bacterial strains i.e. Escherichia coli (ATCC®25922TM) and Staphylococcus aureus (ATCC®25923TM). The study showed that, resazurin microtiter assay (REMA) was simpler, produced quicker result and able to generate reliable data for antimicrobial activity screening compared to the other methods. Moreover, REMA assay is regarded as a suitable assay platform to be implemented for screening of marine invertebrate extracts antibacterial activity since it requires only a small amount of extracts. Based on the MIC values, amongst 126 marine invertebrate extracts screened, 59; 36; 25 and 6 extracts tested against E. coli and 69; 36; 18; and 3 extracts tested against S. aureus were respectively categorized as not active, moderately potential, potential and very potential sample worth to be analyzed further. The ability of the REMA to generate accurate MIC value, which is comparable to the existing antibiotic drug MIC value, will empower researchers to decide whether the extracts worth to be examined further or not. 
Produksi dan Karakterisasi Enzim Selulase Ekstrak Kasar dari Bakteri yang Diisolasi dari Limbah Rumput Laut Isna Rahma Dini; Ifah Munifah
Jurnal Teknologi dan Industri Pertanian Indonesia Vol 6, No 3 (2014): Vol.(6) No.3, October 2014
Publisher : Agricultural Faculty

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (617.634 KB) | DOI: 10.17969/jtipi.v6i3.2315

Abstract

Seaweed waste is a source of bacteria that can produce cellulase enzyme. PMP 0126w isolate is one collection isolate of BBP4BKP obtained from seaweed Glacilaria sp. waste from Pameungpeuk area, Garut, West Java. The aims of this research were to produce and characterize the cellulase enzyme. PMP 0126w isolate was a Gram-positive rod shape bacteria. The isolate had 0.8 cellulolytic index on Carboxymethyl Cellulose (CMC) agar medium. The highest cellulase activity obtained on the third day of fermentation time with a cellulase activity of 0,074 U/mL and specific activity of 0.092 U/mg. Optimum activity of the cellulase enzyme crude extract was pH 5 and 300C. The activity of the cellulase was increased by addition of 5 mM CaCl2 and 10 mM FeCl3 ions and decreased by the addition of 10 mM ZnCl2 ions. The cellulase with the highest activity of 0.237 U/mL was obtained from Glacilaria sp. seaweed waste treated with NaOH 6%. Keywords: cellulose, characterization, production, seaweed waste
Physical Properties of Biodegradable Chitosan-Cassava Starch Based Bioplastic Film Mechanics Kusumawati, Rinta; Syamdidi; Abdullah, Akbar Hanif Dawam; Nissa, Rossy Choerun; Firdiana, Bonita; Handayani, Rini; Munifah, Ifah; Dewi, Fera Roswita; Basmal, Jamal; Wibowo, Singgih
Science and Technology Indonesia Vol. 10 No. 1 (2025): January
Publisher : Research Center of Inorganic Materials and Coordination Complexes, FMIPA Universitas Sriwijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26554/sti.2025.10.1.191-200

Abstract

Petroleum-derived plastics are widely used but pollute the environment significantly. The development of biodegradable plastics is urgently needed to be replaced. The mechanism for making bioplastic films from cassava starch-chitosan/glycerol uses a double-screw extruder process. The film took into account the multi-hydroxyl capacity of starch by combining glycerol (in a ratio of 3:1 w/w) and chitosan (at concentrations of 0.5, 1.0, and 1.5% (w/w). The impact of chitosan involvement on the characteristics of the bioplastic material was studied, including physical, thermal, mechanical, and biodegradability properties. The findings showed that using chitosan as a filler in cassava starch bioplastics resulted in bioplastic films with high compressive capacity and water resistance. The resulting biopolymer’s contact angle was increased by including C-O functional groups in the molecule, as evidenced at a wavelength of 1028 cm-1 of the FTIR spectra. The contact angle was increased from theta = 65.3059 ± 2.7936◦ to theta = 68.6047 ± 3.2391◦. An increase in tensile strength was also observed, indicating increased stiffness compared to chitosan-free bioplastics. The best bioplastic blend was the formulation of cassava starch and glycerol containing 0.5% chitosan. Bioplastic has physical properties of density 0.8625 ± 0.0277 g/mL; contact angle 68.6046 ± 3.2391◦; water uptake 11.0660 ± 0.3709%; tensile strength 2.0181 ± 0.0594 MPa; elongation 54.2243 ± 3.2623%; thermal 137.5◦C; moisture content 4.9464 ± 0.1172%; and the fastest biodegradation rate. The bioplastic synthesized in this study is readily biodegradable in the natural environment, making it highly sustainable and more environmentally friendly, and it can be a viable substitute to reduce the use of petroleum-based bioplastic.
Isolation and Characterization of Cellulose Microfibril (MFC) from Gracilaria sp. with Different Quality Grades Nurhayati; Irianto, Hari Eko; Supriyanto, Agus; Kusumawati, Rinta; Basmal, Jamal; Munifah, Ifah; Setiawati, Natalia Prodiana; Kusumaningrum, Wida Banar; Amanda, Putri; Roziafanto, Ahmad Nandang; Riastuti, Rini; Chalid, Muchamad
Science and Technology Indonesia Vol. 10 No. 3 (2025): July
Publisher : Research Center of Inorganic Materials and Coordination Complexes, FMIPA Universitas Sriwijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26554/sti.2025.10.3.712-724

Abstract

The cellulose found in Gracilaria sp. has not been utilized optimally. This study investigated the characteristics of cellulose and cellulose microfibril (MFC) isolated from three grades of Gracilaria sp. Descriptive tests were performed to determine the quality of eachgrade, including observations on moisture content, ashcontent, CAW,andimpurities. The extraction process involved separating agar from Gracilaria sp., isolating cellulose using 10% NaOH, and bleaching cellulose with 3% NaOCl. The bleached cellulose was then ultrasonicated to produce MFC. Characterization was performed using FTIR, XRD, PSA, STA, DSC, and py-GC/MS. FTIR analysis indicated similar peaks for both cellulose forms but only differed in transmittance intensity. The crystallinity index from XRD analysis was 22–39% for raw Gracilaria sp., 25–46% for cellulose, and 68–89% for MFC. The particle size distribution of MFC mostly ranged between 200–500 nm, with 63.16% frequency. TG analysis showed cellulose decomposition with a Tonset of 231–260oC and a Tmax of 318–326oC. DSC analysis revealed that sonication enhances the polymer structure’s crystallization compared to pre-sonicated cellulose and raw material. The py-GC/MS analysis showed that D-allose and n-Hexadecanoic acid were the major components.
Optimization of Chitosan-Based Edible Film with the Addition of Velvet Bean Aqueous Extract and Cinnamon Essential Oil for Antibacterial Packaging Ariani, Sri Retno Dwi; Sholihah, Khoirur Rohmaniatush; Susilowati, Endang; Setyowati, Widiastuti Agustina Eko; Munifah, Ifah; Rohmatun, Tyas Dwi; Safitri, Nadia Indri
Molekul Vol 20 No 1 (2025)
Publisher : Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.jm.2025.20.1.13671

Abstract

ABSTRACT. The current global warming issue has encouraged a lot of research on edible films. The use of edible films has become an international trend and is now essential for our products to compete in the global market. Bioactive compounds from plants can be incorporated into edible films to enhance their biological activity, including their antibacterial properties. This study developed a chitosan-based edible film with the addition of velvet bean aqueous extract (VAE) and cinnamon essential oil (CEO), aiming for high antibacterial activity. The objectives of this study were to isolate and identify the chemical components of CEO, test its antibacterial activity, find the most optimum formulation of a chitosan-based edible film with VAE and CEO in terms of antibacterial activity (Escherichia coli ATCC 25922 and Staphylococcus aureus ATCC 25923), and analyze the physical and morphological properties of the resulting edible film. The research methods included a literature review and laboratory experiments, with procedures involving the isolation and GC-MS-based identification of CEO chemical components, antibacterial activity testing of CEO, optimization of a chitosan-based edible film formulation with the addition of VAE and CEO based on antibacterial activity test against E. coli ATCC 25922 and S. aureus ATCC 25923 (well diffusion method), and characterization of the best film’s physical properties (thickness, tensile strength, elongation, solubility, WVP, and WVTR) and morphological analysis (SEM method). The results of this study were as follows: (a) CEO contained cinnamaldehyde (52.86%) and 2- methoxycinnamaldehyde (47.06%); (b) CEO had very strong antibacterial activity against E. coli ATCC 25922 and S. aureus ATCC 25923, with inhibition zones of 46.28 mm and 47.95 mm, respectively; (c) the most optimal edible film formulation consisted of chitosan with 30.0% VAE and 4.0% CEO, yielding strong antibacterial activity against E. coli ATCC 25922 (15.50 mm inhibition) and S. aureus ATCC 25923 (16.71 mm inhibition), with a thickness of 0.15 mm, tensile strength of 0.32 Mpa, elongation of 0.32%, solubility of 4.89%, WVP of 8.82 g/m2.h), and WVTR of 3.92 g/m.h. Morphological analysis of the edible film showed a relatively flat and smooth surface with minor cracks in some areas. Keywords: antibacterial packaging, chitosan, cinnamon essential oil, edible film, velvet bean aqueous extract
Co-Authors Agus Supriyanto Akbar Hanif Dawam Abdullah, Akbar Hanif Dawam Amanda, Putri Chalid, Muchamad Dewi Seswita Zilda Dewi, Fera Roswita Diana Natalia Ekowati Chasanah Endang Susilowati Firdiana, Bonita Gintung Patantis Hari Eko Irianto Isna Rahma Dini Jamal Basmal, Jamal Kusumaningrum, Wida Banar Nissa, Rossy Choerun Nurhayati Nurrahmi Dewi Fajarningsih Rini Handayani Rini Riastuti, Rini Rinta Kusumawati, Rinta Rohmatun, Tyas Dwi Roziafanto, Ahmad Nandang Safitri, Nadia Indri Setyowati, Widiastuti Agustina Eko Sholihah, Khoirur Rohmaniatush Singgih Wibowo, Singgih Sri Retno Dwi Ariani Syamdidi TATI NURHAYATI Thamrin Wikanta WIBOWO MANGUNWARDOYO Yusro Nuri Fawzya