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SCREENING AND CHARACTERIZATION OF L-GLUTAMINASE PRODUCED BY BACTERIA ISOLATED FROM SANGIHE TALAUD SEA Ekowati Chasanah; Usman Sumo Friend Tambunan; Tanti Yulianti
Squalen, Buletin Pascapanen dan Bioteknologi Kelautan dan Perikanan Vol 7, No 3 (2012): December 2012
Publisher : Research and Development Center for Marine and Fisheries Product Processing and Biotechnol

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/squalen.v7i3.6

Abstract

L-glutaminase (L-glutamine amidohydrolase, EC 3.5.1.2) is a very important enzyme due to its role as flavor enhancer and antileukemic agent. Salt-tolerant L-glutaminase produced bymarine bacteria is favorable in food industries. This study describes the screening of L- glutaminase producing marine bacteria from Sangihe-Talaud Sea, North Sulawesi, Indonesia.Screening of L-glutaminase was performed using a liquid medium and identification of selected isolate was  performed using molecular-based 16S rDNA. Results showed that there were 7 isolates produced positive results of L-glutaminase, and one of them (II.1 isolate) produced the highest activity, i.e 147.99 U/L, equivalent to the specific activity of 62.32 U/mg. The isolate then selected for further study. Bacterial identification based on 16S rRNA sequencing has revealed that the isolate was 96% similar to Pseudomonas aeruginosa strain CG-T8. Characterization of extracellular L-glutaminase from the II.1 isolate showed that the enzyme worked optimally at temperature  of 37-45 °C and pH 7. The enzyme was stable when NaCl solution was added up to 8% and  began to decrease on addition of NaCl solution of 16% and 20% with relative activity of 79% and 74%, respectively. The effect of metal ions, e.g Mn2+, Mg2+, and Co2+ in the form of chloride salt, were able to increase enzyme activity, whereas the addition of other metal ions (Zn2+, Fe3+, and Ca2+) decreased the activity. The molecular weights of L-glutaminase was estimated around42 kDa and 145 kDa.
ANALISIS HIDROKUINON DALAM KOSMETIK SEDIAAN KRIM YANG BEREDAR DI PASAR PULO GADUNG JAKARTA TIMUR SECARA SPEKTROFOTOMETRI UV-VIS Mega Efrilia; Tria Prayoga; Tanti Yulianti; Pra Panca Bayu Chandra; Eka Fadhila Permatasari
Jurnal Farmasi IKIFA Vol. 5 No. 1 (2026): JURNAL FARMASI IKIFA VOL. 5 NO. 1 APRIL TAHUN 2026
Publisher : Sekolah Tinggi Ilmu Kesehatan IKIFA

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

yang cepat atau instan, hal ini menyebabkan munculnya banyak kecurangan dan pelanggaran di bidang kosmetik dengan mengedarkan produk kosmetik yang tidak memenuhi syarat dan mengandung bahan berbahaya atau dilarang. Hidrokuinon sering disalah gunakan untuk efek memutihkan wajah. Penelitian ini bertujuan untuk menganalisis kandungan hidrokuinon pada sampel kosmetik sediaan krim yang beredar di pasar Pulo Gadung Jakarta Timur secara spektrofotometri UV-Vis. Metode penelitian yang digunakan adalah deskriptif kuantitatif. Hasil penelitian menunjukkan bahwa masih terdapat sampel yang tidak mendapat nomor notifikasi BPOM serta dari hasil pengujian mengandung bahan yang dilarang yaitu hidrokuinon. 13 jenis sampel krim kosmetik yang diperoleh dari Pasar Pulo Gadung Jakarta Timur menunjukkan hasil positif pada uji kualitatif hidroquionon menggunakan pereaksi Ferri klorida dan Benedict. Konfirmasi pengujian menggunakan spektrofotometer UV-VIS menunjukkan kadar kandungan hidrokuionon dalam masing-maisng sampel berada pada rentang 0,123% - 0,132% (b/b).