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Depolimerisasi Kitosan dari Cangkang Udang dengan Enzim Papain dan Iradiasi Sinar Ultraviolet: Depolymerization of Chitosan from Shrimp Shell Using Papain Enzyme and Ultraviolet Light Irradiation Rizfi Fariz Pari; Dewi Mayangsari; Safrina Dyah Hardiningtyas
Jurnal Pengolahan Hasil Perikanan Indonesia Vol 25 No 1 (2022): Jurnal Pengolahan Hasil Perikanan Indonesia 25(1)
Publisher : Masyarakat Pengolahan Hasil Perikanan Indonesia (MPHPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17844/jphpi.v25i1.40311

Abstract

Chitosan is a natural polymer with positive charge that is good to be applied in the pharmaceutical field as a drug carrier. However, commercial chitosan has a large molecular weight and low degree of deacetylation, so its application is limited due to its low solubility in water. This study aims to determine the effect of the depolymerization method of two classes of chitosan on the market. The types of chitosan used to produce chitooligosaccharide are pharmaceutical grade and food grade. The method used were UV-C light irradiation (75 minutes), papain enzyme hydrolysis (15 g/L for 16 hours, 40oC and pH 5) and a combination of UV and enzymes. The results showed that the grade of chitosan and the depolymerization method affected the characteristics of the product. The three methods used in pharmaceutical grade chitosan produce chitooligosaccharide which dissolved directly in water with a neutral pH. Chitooligosaccharide had yield of >62%, whiteness degree of 99.33%, molecular weight <5.5 KDa, and degree of deacetylation >85%. Food grade chitosan which has been depolymerized by different methods cannot be dissolved in water with a neutral pH. Food grade chitosan through the combination of UV+enzyme depolymerization has the same deacetylation quality as pharmaceutical grade chitosan.
Ekstraksi Polisakarida Ulvan dari Rumput Laut Ulva lactuca Berbantu Gelombang Ultrasonik pada Suhu Rendah : Ultrasonic Wave Assisted Extraction of Ulvan Polysaccharide from Ulva Seaweed at Low Temperature Wahyu Ramadhan; Uju Uju; Safrina Dyah Hardiningtyas; Rizfi Fariz Pari; Nurhayati Nurhayati; Devani Sevica
Jurnal Pengolahan Hasil Perikanan Indonesia Vol 25 No 1 (2022): Jurnal Pengolahan Hasil Perikanan Indonesia 25(1)
Publisher : Masyarakat Pengolahan Hasil Perikanan Indonesia (MPHPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17844/jphpi.v25i1.40407

Abstract

Ulvan merupakan polisakarida bersulfat utama dari rumput laut Ulva yang memiliki aktivitas biologis penting. Umumnya ulvan diekstrak menggunakan suhu tinggi dan dengan rentang waktu yang lama, oleh karena itu diperlukan metode ekstraksi alternatif yang dapat menghasilkan ulvan yang memiliki kualitas yang baik namun tetap mendukung konsep efisiensi energi. Salah satu metode green extraction yang dapat mengurangi penggunaan pelarut organik dan energi yang tinggi selama ekstraksi adalah dengan bantuan gelombang ultrasonik. Tujuan penelitian ini adalah untuk menentukan waktu, suhu dan jenis pelarut dengan bantuan ultrasonik yang paling baik dalam menghasilkan rendemen dan karakteristik ulvan yang sesuai dibandingkan dengan ulvan yang diekstraksi dengan metode konvensional atau tanpa bantuan ultrasonikasi. Pada penelitian tahap awal digunakan kombinasi antara jenis pelarut (akuades, HCl dan NaOH) dengan kondisi ekstraksi suhu rendah (50, 60, dan 70℃) selama 45 dan 90 menit dengan bantuan ultrasonik. Rendemen tertinggi ulvan diperoleh dari ekstraksi menggunakan NaOH pada suhu 70℃ selama 90 menit dengan bantuan ultrasonik sebesar 16,90±0,45%. molekul dan viskositas ulvan yang dihasilkan dengan berbantu ultrasonik menunjukkan penurunan nilai dibandingkan dengan tanpa ultrasonikasi. Hasil Fourier-transform infrared spectroscopy mengkonfirmasi pita serapan grup sulfat yang merupakan residu gula rhamnose pada panjang gelombang 1125 cm-1 dan C-O-S pada 983 cm-1. Ulvan yang diekstrak dengan sonikasi menunjukkan kandungan sulfat 39, 58 dan 53% untuk masing- masing pelarut akuades, HCl dan NaOH. Perlakuan ultrasonikasi memberikan pengaruh pada kandungan sulfat 2-14% lebih tinggi dibandingkan dengan sulfat yang dihasilkan dari metode konvensional.
PENILAIAN POTENSI BIOMASSA SEBAGAI ALTERNATIF ENERGI KELISTRIKAN Petir Papilo; Kunaifi Kunaifi; Erliza Hambali; Nurmiati Nurmiati; Rizfi Fariz Pari
Jurnal PASTI (Penelitian dan Aplikasi Sistem dan Teknik Industri) Vol 9, No 2 (2015): Jurnal PASTI
Publisher : Universitas Mercu Buana

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (164.57 KB)

Abstract

Pemanfaatan biomassa sebagai sumber daya listrik merupakan salah satu solusi yang dapat dikembangkandalam rangka meningkatkan rasio elektrifikasi dan mewujudkan ketahanan energi nasional. Untuk itu sebagai langkah awal bagi menguji kelayakan pengembangan biomassa sebagai sumber pembangkit tenaga listrik, diperlukan analisis tentang potensi sumber daya, terutama dari beberapa komoditas unggulan, baik dari sisa hasil pertanian maupun perkebunan. Penelitian ini bertujuan untuk mengetahui tingkat potensi biomassa berdasarkan ketersediaan pasokan sumber daya yang tidak termanfaatkan yang dapat diperoleh dari sisa hasil pertanian. Beberapa sisa hasil pertanian dan perkebunan yang menjadi target penilaian antara lain adalah jerami dan sekam padi sawah, jerami dan sekam padi ladang, batang dan tongkol jagung, batang ubi kayu, serta serat, cangkang, tandan kosong, kernel dan limbah cair yang terdapat di perkebunan kelapa sawit. Hasil analisis dengan pendekatan statistik, menunjukkan bahwa total energi teoritis biomassa yang dapat dihasilkan adalah sebesar 77.466.754,8 Gj/Tahun. Secara teoritis berpotensi menghasilkan energi listrik sebesar 21.518.542,8 MWh/Tahun.Kata Kunci: Biomassa, Potensi Teoritis, Rasio Elektrifikasi
Recent Advances in the Use of Transglutaminase in Cheese Production Warsono El Kiyat; Evlyn Laurenthia; Janice Michaela; Rizfi Fariz Pari
ASEAN Journal on Science and Technology for Development Vol. 38 No. 2 (2021): Repurpose & Redefine
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29037/ajstd.675

Abstract

Various enzymes are used in the food industry to improve product quality. The enzyme transglutaminase is used to modify proteins in various foods through the formation of inter- and intramolecular epsilon-(gamma-glutamyl) lysine bonds. Previous studies have revealed that using transglutaminase in cheese production can increase the yield and enhance the characteristics of various types of cheese. However, the enzyme concentration, temperature, and pH influence the quality of cheese. This review aimed to discuss the potential of transglutaminase in cheese production. Our analysis showed that transglutaminase catalyzes the formation of covalent bonds between milk proteins (crosslinking), which results in a greater amount of casein trapped from whey and a higher yield of curd. Transglutaminase can also reduce production costs without negatively affecting cheese quality. These findings should prove useful in developing cheese products to improve consumer satisfaction.
Profil asam lemak minyak dari jeroan ikan nila dan mas dengan rasio pelarut yang berbeda: Fatty acid profiles of fish oil from tilapia and carp with different solvent ratio Sugeng Heri Suseno; Rizfi Fariz Pari; Bustami Ibrahim; Rizki Tri Kurnia Ramadhan; Desi Listiana; Farah Nurjannah; As Syaffa Amalia Adha
Jurnal Pengolahan Hasil Perikanan Indonesia Vol 26 No 3 (2023): Jurnal Pengolahan Hasil Perikanan Indonesia 26 (3)
Publisher : Department of Aquatic Product Technology IPB University in collaboration with Masyarakat Pengolahan Hasil Perikanan Indonesia (MPHPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17844/jphpi.v26i3.45781

Abstract

Extraction of fish oil from the by-products of tilapia and carp can be achieved using an appropriate extraction process. The purpose of this study was to determine the proportion of acetone and fish oil solvents in relation to the oxidation parameters, iodine number, and concentration profile of omega-3 fatty acids present in tilapia and goldfish oil. This study utilized a completely randomized design, in which the treatment ratios of acetone and oil solvent were varied as 3:1, 4:1, 5:1, 6:1, and 7:1 (v/v). The following parameters were examined: the chemical composition, free amino acids, acid value, peroxide value, p-anisidine, total oxidation, iodine value, and fatty acid profile.The omega-3 levels in tilapia and carp were 1.20% and 1.53%, respectively. An Iodine Number Test was employed to identify the most effective solvent treatment, revealing a 5:1 (v/v) ratio as the optimal solution. The increase in the value of omega-3 in tilapia oil amounted to 1.76%, whereas that in carp oil increased from 0.78% to 2.96%, resulting in an average increase of 2.31%.
Produksi Tanaman Selada Merah Secara Vertikultur dengan Pemberian POC Jakaba : Production of Red Lettuce Plant by Verticulture Using Jakaba POC A. Farhanah; Hamzah, Faisal; Kaharuddin; Atika; Fariz Pari, Rizfi
Jurnal Agrisistem Vol. 20 No. 1 (2024): Jurnal Agrisistem
Publisher : Unit Penelitian dan Pengabdian Masyarakat Politeknik Pembangunan Pertanian Gowa

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52625/j-agr.v20i1.309

Abstract

Currently, sustainable agriculture is needed to meet human needs in the long term, one of which is the use of organic matter. Jakaba is currently a very interesting novelty to test on plants. Therefore, this study was carried out to determine the effect of Jakaba fertilizer on the production of red lettuce plants verticulturally.The method used was a two-factor Random Group Design with nine types of treatments and three repeats. The first factor is POC Jakaba and the second factor is NPK fertilizer.The results showed that the application of jakaba fertilizer combined with NPK had a significant effect on increasing the height, number of leaves, root volume, and fresh weight of verticulture red lettuce plants and the application of jakaba fertilizer as much as 50% combined with NPK as much as 1 g per plant was effective in increasing the fresh weight of verticulture red lettuce plants. The conclusion obtained is that there is an influence of POC Jakaba on increasing the production of red lettuce plants verticulturally, especially in increasing the fresh weight of the plant.
Pre-treatment of Lobster Shell Using Hydrochloric Acid for Nanochitosan Production Safrina, Dyah; Ulfa Safitri, Anisa; Suptijah, Pipih; Fariz Pari, Rizfi
Journal of Marine and Coastal Science Vol. 12 No. 2 (2023): JUNE
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jmcs.v12i2.45883

Abstract

Lobster shells are a by-product that can be used as a raw material for making chitin and chitosan. However, lobster shells contain a high amount of minerals that cover the chitin polymer on the shell. This chitin can be converted into chitosan by deacetylation process. Efforts that can be made to increase the efficiency of the chitosan extraction process from lobster shells are by pre-treatment using hydrochloric acid. The purpose of this study was to determine the pre-treatment time of lobster shells with hydrochloric acid and to produce nanochitosan from lobster shells. The results of this study indicate that the pretreatment time has a significant effect on the yield value of chitosan. The highest yield of chitosan was produced from the acid pre-treatment with a soaking time of 120 hours with a value of 15,6%. The quality of lobster chitosan from the best treatment has met the quality requirements based on SNI. Nanochitosan was successfully synthesized by ionic gelation method with a particle size of 357,76 nm and spherical shape based on SEM photos. Lobster nanochitosan has the potential to be applied in various fields, such as food, agriculture,and biomedical.
Potency of Cellulolytic Bacteria in Ulva lactuca from Ujung Genteng Beach, Sukabumi, Indonesia Pari, Rizfi Fariz; Hardiningtyas, Safrina Dyah; Supraba, Ranula
Journal of Marine and Coastal Science Vol. 12 No. 2 (2023): JUNE
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jmcs.v12i2.46102

Abstract

Fishery Ulva lactuca is a potential green seaweed species that causes green tides and has not been utilized. U. lactuca has the potency to attract cellulolytic bacteria due to its high carbohydrate content. U. lactuca is commonly found on the southern seacoast of Java Island, one of which is Ujung Genteng Beach, Sukabumi, Indonesia. In this research, we investigated the potential of cellulolytic bacteria from U. lactuca. Sources of bacteria are coming from the environment, the surface of the seaweed, and inside the seaweed (endophytes). To be able to determine the origin of the potential bacteria, the total plate count (TPC) was calculated with treatment without washing, washing with distilled water, and washing with a combination of distilled water and alcohol. From these bacteria, cellulolytic activity was tested using CMC media and morphological characterization. The most bacteria were found in the treatment without washing, as much as 7.48 Log Cfu/ml. The washing process reduced the TPC to 5.88 and 5.7 Log Cfu/ml in the treatment of washing with distilled water and washing with a combination of distilled water and alcohol, so that endophytic bacteria were obtained. The bacterial isolate with the highest cellulolytic potential was UW 1(1)1 5A1 from the non-washing treatment with a cellulolytic index of 2.25. These Gram-positive bacteria were in the form of filamentous lobates with a matte cream color with cocci-shaped cells. Meanwhile, the potential endophytic bacteria were Gram-positive coccus bacteria UW 3(1)1.2 with a cellulolytic index of 0.85. Both isolates have the potency to degrade cellulose.