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All Journal Jurnal Online Mahasiswa (JOM) Bidang Matematika dan Ilmu Pengetahuan Alam Jurnal Pengabdian UntukMu NegeRI IJOEP : International Journal of Ecophysiology Dinamika Pertanian
Dewi Indriyani Roslim
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Religion Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemistry Computer Science & IT Economics, Econometrics & Finance Education Immunology & microbiology Law, Crime, Criminology & Criminal Justice Mathematics Social Sciences

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Journal : IJOEP : International Journal of Ecophysiology

 1 
cDNA Actin Isolated From Pandanus Sp. Dewi Indriyani Roslim
International Journal of Ecophysiology Vol. 1 No. 2 (2019): International Journal of Ecophysiology
Publisher : Talenta Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (836.888 KB) | DOI: 10.32734/ijoep.v1i2.1271

Abstract

Actin is one of the reference genes that is often used as an internal control in gene expression analysis. This study aimed to isolate actin cDNA from Pandanus sp originated from Riau. Fresh leaves Pandanus sp. Lake Kajuik, Langgam District, Pelalawan Regency, Riau Province. Isolation of RNA, synthesis of total cDNA, amplification of actin genes used McDowell's designed degenerate primer (PlAc46S-20/PlAc245N-20), electrophoresis, sequencing, and data analysis. Actin cDNA fragments obtained were 353 pb in size, registered at GenBank and encoded 117 amino acids. Actin cDNA fragment consists of two exons and one introne. Specific actin primers from Riau Pandanus sp. can be designed based on sequences obtained for the purpose of analyzing certain gene expressions.
Co-Authors Adiwirman Dede Aryani Novitasari Della Rinarta Desnilia ' Dita Deanesia Dwi Wulan Elfrida Oktavia Endah Budi Lestari Ermi Ningsih Fadel Nugraha Fathurrahman Fathurrahman Fitmawati ' Fitmawati Fitmawati Herman ' Herman Herman Herman Herman Indah Mulyasari M. Adi Zulkifli Misriyani Misriyani Murtiana Caniago Nazaruddin Nazaruddin Nery Sofiyanti Okyarni Nuzila Rahmi Anandia Rinda Anggini Rini Restiani Rizka Suci Akmando Rotupa Juliana Manurung Roza Elvyra Shinta Oktavia Yolla Putri Ardila Yusfiati Yusfiati