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Analisis Sebagian Sekuen Gen Ferritin2 pada Padi (Oryza sativa L.) Indragiri Hilir, Riau Nugraha, Fadel; Indriyani Roslim, Dewi; Putri Ardilla, Yolla; -, Herman
Biosaintifika: Journal of Biology & Biology Education Vol 6, No 2 (2014): September 2014
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v6i2.3102

Abstract

Ion Fe bebas sangat beracun bagi tanaman, karena dapat membentuk radikal bebas di dalam sel. Walaupun demikian, tanaman memiliki mekanisme untuk mempertahankan homeostasis Fe di dalam sel yang melibatkan protein ferritin. Penelitian ini bertujuan untuk menganalisis dan membandingkan sekuen nukleotida gen ferritin2 dari dua genotipe padi lokal (Bakung dan Serei) dari Indragiri Hilir, Riau dengan varietas padi rawa unggul tahan cekaman kelebihan Fe (Siam Sintanur), padi unggul tidak tahan cekaman kelebihan Fe (IR64), dan data sekuen nukleotida kultivar Nipponbare. Metode penelitian meliputi isolasi DNA toal dari daun segar tanaman padi menggunakan metode CTAB dan amplifikasi DNA (PCR) menggunakan primer OsFer_F3 (forward) dan Gross_R (reverse). Produk PCR kemudian disekuensing dan disejajarkan. Pada penelitian ini telah diperoleh fragmen dari gen ferritin2 dari kelima genotype atau varietas padi yang diuji, yang berukuran sekitar 1200 pb. Analisis pensejajaran menunjukkan terdapat 56 SNP (single nucleotide polymorphism) pada sekuen nukleotida gen ferritin2 tersebut. Bakung menunjukkan kedekatan yang tinggi dengan Nipponbare, diikuti dengan IR64, Siam Sintanur, dan Serei. Kemungkinan Bakung merupakan genotipe padi lokal dari Indragiri Hilir, Riau yang tahan cekaman kelebihan Fe.Free Fe ions are highly toxic to plants, because it can form free radicals in the cells. However, plants have mechanisms to maintain Fe homeostasis in the cells involving ferritin proteins. This study was aimed to analyze and to compare the nucleotide sequence of ferritin2 gene in two local rice genotypes (namely Bakung and Serei) from Indragiri Hilir, Riau and in Fe overload-tolerant rice variety (Siam Sintanur), Fe overload-sensitive rice variety (IR64), as well as the nucleotide sequence of Nipponbare rice cultivar. The research methods consisted of DNA isolation from fresh leaves of rice plants using CTAB method and DNA amplification (PCR) using a couple of primers, OsFer_F3 (forward) and Gross_R (reverse). The PCR products were then sequenced and aligned. DNA fragments of ferritin2 gene with length of approx. 1200 bp were obtained from those four rice varieties or genotypes tested. Alignment revealed 56 SNPs (single nucleotide polymorphisms) in the ferritin2 gene sequences. Bakung showed close distance with Nipponbare, followed by IR64, Siam Sintanur, and Serei. It wa suggested that Bakung was Fe overload-tolerant local rice genotype from Indragiri Hilir, Riau.
Analisis Sebagian Sekuen Gen Ferritin2 pada Padi (Oryza sativa L.) Indragiri Hilir, Riau Fadel Nugraha; Dewi Indriyani Roslim; Yolla Putri Ardilla; Herman -
Biosaintifika: Journal of Biology & Biology Education Vol 6, No 2 (2014): September 2014
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v6i2.3102

Abstract

Ion Fe bebas sangat beracun bagi tanaman, karena dapat membentuk radikal bebas di dalam sel.  Walaupun demikian, tanaman memiliki mekanisme untuk mempertahankan homeostasis Fe di dalam sel yang melibatkan protein ferritin.  Penelitian ini bertujuan untuk menganalisis dan membandingkan sekuen nukleotida gen ferritin2 dari dua genotipe padi lokal (Bakung dan Serei) dari Indragiri Hilir, Riau dengan varietas padi rawa unggul tahan cekaman kelebihan Fe (Siam Sintanur), padi unggul tidak tahan cekaman kelebihan Fe (IR64), dan data sekuen nukleotida kultivar Nipponbare.  Metode penelitian meliputi isolasi DNA toal dari daun segar tanaman padi menggunakan metode CTAB dan amplifikasi DNA (PCR) menggunakan primer OsFer_F3 (forward) dan Gross_R (reverse).  Produk PCR kemudian disekuensing dan disejajarkan.  Pada penelitian ini telah diperoleh fragmen dari gen ferritin2 dari kelima genotype atau varietas padi yang diuji, yang berukuran sekitar 1200 pb.  Analisis pensejajaran menunjukkan terdapat 56 SNP (single nucleotide polymorphism) pada sekuen nukleotida gen ferritin2 tersebut.  Bakung menunjukkan kedekatan yang tinggi dengan Nipponbare, diikuti dengan IR64, Siam Sintanur, dan Serei.  Kemungkinan Bakung merupakan genotipe padi lokal dari Indragiri Hilir, Riau yang tahan cekaman kelebihan Fe. Free Fe ions are highly toxic to plants, because it can form free radicals in the cells.  However, plants have mechanisms to maintain Fe homeostasis in the cells involving ferritin proteins.  This study was aimed to analyze and to compare the nucleotide sequence of ferritin2 gene in two local rice genotypes (namely Bakung and Serei) from Indragiri Hilir, Riau and in Fe overload-tolerant rice variety (Siam Sintanur), Fe overload-sensitive rice variety (IR64), as well as the nucleotide sequence of Nipponbare rice cultivar.  The research methods consisted of DNA isolation from fresh leaves of rice plants using CTAB method and DNA amplification (PCR) using a couple of primers, OsFer_F3 (forward) and Gross_R (reverse).  The PCR products were then sequenced and aligned.  DNA fragments of ferritin2 gene with length of approx. 1200 bp were obtained from those four rice varieties or genotypes tested.  Alignment revealed 56 SNPs (single nucleotide polymorphisms) in the ferritin2 gene sequences.  Bakung showed close distance with Nipponbare, followed by IR64, Siam Sintanur, and Serei.  It wa suggested that Bakung was Fe overload-tolerant local rice genotype from Indragiri Hilir, Riau.
ANALISIS SEKUEN INTRON 1 SAMPAI SEBAGIAN EKSON 4 DARI GEN FERITIN2 PADA TIGA GENOTIPE PADI (Oryza sativa L.) LOKAL INDRAGIRI HILIR, RIAU Herman Herman; Yolla Putri Ardila; Fadel Nugraha; Dewi Indriyani Roslim
DINAMIKA PERTANIAN Vol. 29 No. 1 (2014): Jurnal Dinamika Pertanian Edisi April 2014
Publisher : UIR Press

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Iron toxicity is one of the limiting factors of rice crop production in lowland area. Iron homeostasis in cell involves the role of ferritin protein. The objective of this study is to analyze potion of first intron to fourth exon sequence of local rice varieties from Indragiri Hilir, Riau Province. Plant material were three local rice genotype, namely Bakung, Siputih, and Serei, and two rice varieties consisted of IR64 (Fe toxicity-sensitive rice variety) and Siam Sintanur (Fe toxicity-tolerant rice variety). Methods include DNA isolation, PCR (Polymerase Chain Reaction), electrophoreses, and sequencing. The DNA sequence was analyzed using BLASTn and ClustalW2 programs. This research got DNA fragments of ferritin2 gene sizing of about 850 bp, 1200 bp, and 1500 bp. Sequence analysis of the 1200 bp DNA fragment showed the presence of 49 SNPs scattered portion of first intron to fourth exon. Bakung rice genotype had very close proximity to Siam Sintanur and Nipponbare compared with IR64, while Serei and Siputih rice genotypes had a great distance to Siam Sintanur and Nipponbare. The result concluded that Bakung rice genotype may be more tolerant to Fe-toxicity than Siputih and Serei.
Sosialisasi Tanaman Penurun Gula Darah Di Desa Koto Ringin Kecamatan Mempura Kabupaten Siak Radith Mahatma; Dyah Iriani; Yusfiati, Yusfiati; Nery Sofiyanti; Roza Elvyra; Fadel Nugraha
Jurnal Pengabdian UntukMu NegeRI Vol. 8 No. 3 (2024): Pengabdian Untuk Mu negeRI
Publisher : LPPM UMRI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37859/jpumri.v8i3.8055

Abstract

Negative impact of the development over time in the health aspect is the change in lifestyle or unhealthy dietary habits, particularly through the consuming of instant foods, which can trigger diabetes. Excessive sugar consumption can lead to increasing weight and blood sugar levels, and potentially cause type-2 diabetes (DM). The community of Koto Ringin Village has developed a family medicinal plant program (TOGA) as part of a strategy to improve community health. This program demonstrates proactive steps in addressing health challenges in Koto Ringin Village, both through disease management and community-based health initiatives like TOGA. The purpose of this community service is to improve the understanding of the PKK (Family Welfare Movement) members of Koto Ringin Village by educating them about plants that are useful for lowering blood sugar levels. This community service is also intended for transferring knowledge from the academic environment to the community at large, especially to the members of PKK Koto Ringin Village, Mempura District, Siak Regency, Riau Province. This activity was attended by 18 women from the PKK of Koto Ringin. The results of the activity showed that the participants’ knowledge improved from 41.1 to 90.0.
Pemanfaatan Video Pembelajaran dalam Meningkatkan Motivasi Belajar Mahasiswa pada Mata Kuliah Biologi Dasar Syafruddin, Wardatul Jannah; Nugraha, Fadel
Spizaetus: Jurnal Biologi dan Pendidikan Biologi Vol 6 No 2 (2025): Spizaetus: Jurnal Biologi dan Pendidikan Biologi
Publisher : Program Studi Pendidikan Biologi, FKIP, Universitas Nusa Nipa

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55241/spibio.v6i2.568

Abstract

Complex Basic Biology course materials and learning processes that still use manual textbooks make it difficult for students to follow the learning process on campus. Learning videos are audio-visual media used to deliver educational or training materials in an interesting and effective way. Through learning videos, the delivery of information becomes more interactive, attracts attention, and makes it easier to understand complex concepts. Biology learning videos are made with the aim of making it easier for students to understand the material so that they can increase their interest and motivation in learning biology, especially Basic Biology courses. This study involved 76 undergraduate biology students taking a Basic Biology course. It used both qualitative and quantitative methods. Data was collected through a questionnaire where students evaluated the learning videos. The validity test showed a significant value of 0.00 < 0.05, and the reliability test gave a value of 0.074 > 0.07, meaning the questionnaire was valid and reliable. The results of the data analysis showed that more than 80% of students agreed and strongly agreed that the biology learning videos helped them understand the material better. In conclusion, learning videos can increase students' interest and motivation in studying biology, especially in Basic Biology courses.
Effectiveness of Non-SLS Shampoo Based on VCO and Tectona grandis Leaf Extract for Alopecia Treatment Nugraha, Fadel; Halim, Michelle Graciella Jacinta; Sihotang, Naomi Chiko Putri; Febrianto, Yudha; Safniyeti, Safniyeti; Wulandari, Nindya
Jurnal Biologi Tropis Vol. 25 No. 3 (2025): Juli-September
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v25i3.9150

Abstract

Hair loss is a common condition found in various groups. Various factors can cause hair loss, including genetic factors, hormonal imbalance, stress, nutritional deficiencies, and certain medical conditions. The type of disorder that occurs can be hair loss (effluvium) or hair loss disorders to baldness (alopecia). The study aims to determine the effectiveness of non-SLS shampoo preparations with a combination of teak leaf extract (Tectona grandis) and virgin coconut oil (Cocos nucifera) in overcoming hair loss and increasing hair growth rate. The shampoo preparation was tested on mice with 4 treatment groups consisting of 1 control group and 3 groups of mice that were given treatment (P1 containing 1,5% teak leaf extract and 0% VCO, P2 containing 2% teak leaf extract and 0,15% VCO, and P3 containing 2,5% teak leaf extract and 0,30% VCO). The trial on mice was conducted for 21 days and the calculation of the length of the mouse fur using a caliper was carried out periodically for 7 days. The results of study showed that the third formula showed an increase in the rate of hair growth. The effect of shampoo that showed most significant difference was P3 with 2,5% teak leaf extract and 0,30% coconut oil. Shampoo products containing teak leaf extract and coconut oil have been proven to have potential to increase rate of hair growth. This shampoo formula can also reduce hair loss supported by scientific journals reporting that teak leaf extract can inhibit work of steroid 5-α reductase (5SAR) and secretion of Interleukin-1 Beta (1L-1β), where both compounds have a negative impact on hair growth.
Studi Etnobotani: Inventarisasi dan Pemanfaatan Tumbuhan Obat di Desa Pelabai Kabupaten Lebong, Provinsi Bengkulu Safniyeti, Safniyeti; Astuti, RR Sri; Fatimatuzzahra, Fatimatuzzahra; Kasrina, Kasrina; Nugraha, Fadel
Journal of Natural Sciences Vol 6, No 2 (2025): Journal of Natural Sciences Juli
Publisher : Mahesa Research Center

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.34007/jonas.v6i2.941

Abstract

Traditional medicinal plants hold a crucial role in public health in rural areas where medical facilities are limited. People often rely on plants as medicinal raw materials. This study aims to investigate ethnobotany and inventory the types of medicinal plants used by the residents of Pelabai Village, Lebong Regency, Bengkulu Province. Data were collected through interviews using snowball sampling and analyzed desriptively. Semi-structured interviews with open-ended questions and direct observations of daily activities were conducted. Twenty respondents who knew, understood, and used plants as alternative medicine were selected. The study identified 30 plant species in the village used for traditional medicine. Leaves were the most commonly used plant part for medicinal purposes (76.67%), followed by stems (23.33%), rhizomes (13.33%), roots (10%), sap (6.67%), and other parts such as gel, flowers, fruit, and stem bark (3.33%). The plants were prepared for medicinal use through boiling, mashing, grating, dripping, squeezing, sticking, chewing, or direct consumption. Plant were used to treat various minor ailments including nausea, aches, skin diseases, beauty enhancement, respiratory issues, cholesterol, and hypertension.
The Effect of Gross Domestic Product, Energy Intensity, Population, and Urbanization on Total CO2 Emissions (Study on Brics Member Countries) Hati, Dhinny Faadhilah Handayani Permata; Nugraha, Fadel; Sasana, Hadi
AFEBI Economic and Finance Review Vol. 7 No. 2 (2022): December
Publisher : Asosiasi Fakultas Ekonomi dan Bisnis Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

The balance between accelerating the rate of economic growth and protecting the quality of the environment is one of the challenges faced by both developed and developing countries. Without a commitment to carry out sustainable development, the use of natural resources and the environment will increase. This increase in utilization increases CO2 emissions every year from the combustion of fossil fuels. The increase in CO2 emissions is triggered by economic development activities. The purpose of this study is to examine the effect of the variables of gross domestic product, energy intensity, population, and urbanization on total CO2 emissions in 2005 to 2018 in BRICS member countries. This research is a quantitative research type. The data used is secondaru data obtained through library search with panel data totalling 70 observations. The data analysis technique used is multiple linear regression analysis with the analysis model used in this study is panel data regression with Fixed Effect Model. The results of this study indicate that the variables of energy intensity and population have a positive and significant effect, while the variables of gross domestic product and urbanization do not have a significant effect. Simultaneously, the independent variable affects the dependent variable.