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All Journal JBIO: jurnal biosains (the journal of biosciences) Biogenesis: Jurnal Ilmiah Biologi Jurnal Geografi Berkala Penelitian Hayati
Eko Prasetya
Department Biology, Faculty Of Mathematics And Natural Sciences, Universitas Negeri Medan
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Earth & Planetary Sciences Education Environmental Science Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology Social Sciences Other

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Journal : JBIO: jurnal biosains (the journal of biosciences)

 1 
DETECTION OF PIG DNA FRAGMENTS IN HALAL UNLABLED LIPSTICK SAMPLES USING CONVENTIONAL PCR Misbakhul Munir; Siti Malihatus Sa'adah; Siti Latifa; Nabila Ayu; Oki Rahmatirta W; Najwa Maulidina P; Ameliora C E; Eko Prasetya; Yuanita Rachmawati
JBIO: jurnal biosains (the journal of biosciences) Vol 7, No 1 (2021): Jurnal Biosains
Publisher : Universitas Negeri Medan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24114/jbio.v7i1.23707

Abstract

From a Muslim perspective, it is very important to know the content, raw materials, and processing of the raw materials used in the cosmetic products used. One type of cosmetics that is most often used is lipstick. However, many lipsticks circulating in Indonesia are not equipped with a halal logo. One of the ingredients for lipstick is pork derivatives. These pig derivatives can be detected using PCR. Based on this background, this study aims to test the presence of pig DNA in lipstick samples that have not been certified halal on the market using 4 combinations of pig DNA fragments coding primers by using the conventional PCR method. Five commercial lipstick samples were selected by purposive sampling. DNA isolation was carried out according to the Wizard Promega Universal Kit. The PCR process was carried out with temperature optimization as follows: Predenaturation 98oC: 2 minutes, denaturation of 95oC: 30 seconds, Annealing 61oC: 30 seconds, Extension 72oC: 40 seconds, and Postextension 72oC: 3 minutes. The results showed that of the 5 samples tested by PCR using 5 kinds of primer combination, none of the samples were suspected to contain pork DNA. DNA isolation is the most difficult step in the lipstick sample detection process. Even though the detection result is negative, it is necessary to carry out further tests which become the Gold Standard of DNA-based testing using Real Time PCR.
DNA BARCODING EDELWEISS (Anaphalis longifolia) ASAL SUMATERA UTARA MENGGUNAKAN SEKUEN GEN maturase K Eko Prasetya; Hary Prakasa; Miftahul Jannah; Yuanita Rachmawati
JBIO: jurnal biosains (the journal of biosciences) Vol 6, No 3 (2020): Jurnal Biosains
Publisher : Universitas Negeri Medan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24114/jbio.v6i3.22403

Abstract

Anaphalis longifolia merupakan anggota dari family Asteraceae yang tersebar di dataran tinggi Eropa, Amerika, hingga Asia. Penelitian tentang tanaman ini masih terbatas pada studi habitat, sedangkan penelitian terkait identifikasi molekuler masih belum dilakukan. Penelitian ini bertujuan untuk menganalisis DNA barcode dari A. longifolia menggunakan sekuen matK gene. Sampel yang diperoleh dari Sumatera Utara kemudian di Isolasi DNA, di amplifikasi menggunakan primer spesifik, lalu disequencing. Hasil sequencing dianalisis menggunakan program Molecular Evolution Genetics Analysis (MEGA) Version X. Hasil penelitian menunjukkan bahwa sekuen matK gen berhasil diamplifikasi pada panjang 800-850 kb. Hasil analisis pohon filogenetik menunjukkan bahwa sekuen matK gene dapat mengelompokkan A. longifolia. Pada sekuen matK gene A. longifolia, AT content lebih tinggi dibandingkan dengan GC conten. Jarak genetik yang diperoleh berkisar 0-0.0014. Hasil analisis alignment sekuen matK gene menunjukkan terdapat 1521 karakter yang dapat diamati, 1403 karakter conserved site, 118 karakter variable site, 9 karakter parsimony informative site, dan 7 karakter single nucleotide polymorphism (SNP) site. Sekuen matK gene dapat digunakan sebagai DNA barcoding untuk mengidentifikasi A. longifolia. Hasil penelitian ini diharapkan dapat memberikan informasi penting dalam konservasi A. longifolia.
DNA BARCODING of Zingiber loerzingii Valeton USING Ribulose-1,5-biphosphate Carboxylase-Oxygenase Large subunit Gene (rbcL) GENE LOCUS Ladiez Rahmayani Sagala; Lazuardi Lazuardi; Fauziyah Harahap; Kartika Manalu; Zahratul Idami; Eko Prasetya
JBIO: jurnal biosains (the journal of biosciences) Vol 7, No 3 (2021): Jurnal Biosains
Publisher : Universitas Negeri Medan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24114/jbio.v7i3.28921

Abstract

DNA barcode is one of the molecular techniques used to identify and classify living things. Z. loerzingii is currently reported as a rare and endemic plants that is only found in North Sumatra. Scientific studies for Z. Loerzingii is measly done so that the information about this plant is limited while a clear identity on a plant is essential to discover the potential. This study aims to determine the molecular characteristics of Z. loerzingii by using DNA barcodes and assessing the phylogenetic relationship based on the rbcL gene locus. DNA was isolated with a commercial kit. The rbcL gene locus in the chloroplast genome of Z. loerzingii amplified using the Polymerase Chain Reaction technique to produce amplicon with the length approximately 600 bp. Consensus sequence merging generate a sequence with 576 bp length. The phylogenetic tree reconstruction was carried out using the Neighbor-Joining method and the Kimura-2-Parameter calculation model showed Z. loerzingii included in the monophyletic group with Zingiber mioga and Zingiber officinale as the sister taxa. The results for the molecular diversity analysis of Z. Loerzingii point out that in all samples of Z. loerzingii which collected from Cagar Alam Sibolangit have no molecular or genetic diversity. Therefore, it can be concluded that DNA barcoding with the rbcL gene locus can be used as a method to identify Z. loerzingii molecularly and efficient in determining their phylogenetic with other species.
Co-Authors Aida Fitriani Sitompul Albert D Situmorang Ameliora C E Annisa Afiva Erwin Pane Fauziyah Harahap Fauziyah Harahap Gultom, Endang Sulistyarini Hary Prakasa Hasnaul Maritsa Hasruddin Kartika Manalu Ladiez Rahmayani Sagala Lazuardi Lazuardi Maimunah Maimunah Miftahul Jannah Miftahul Jannah Miftahul Jannah Misbakhul Munir Muhammad Ridha Syafii Damanik Muhammad Rifqi Hariri Nabila Ayu Najwa Maulidina P Nida Afifah Oki Rahmatirta W Qurrota Ayun Siti Latifa Siti Malihatus Sa'adah Teguh Febri Sudarma Tri Hartanti Yuanita Rachmawati Zahratul Idami Zulheri Noer