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All Journal Indonesian Journal of Clinical Pathology and Medical Laboratory (IJCPML) Jurnal Farmasi
RUBEN DHARMAWAN
Faculty of Medicine, Sebelas Maret University
Biochemistry, Genetics & Molecular Biology Dentistry Health Professions Medicine & Pharmacology Neuroscience Nursing Public Health

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MODIFIKASI PRINSIP PEMERIKSAAN β-D-GLUCAN UNTUK MENDETEKSI CANDIDA ALBICANS DALAM SERUM Dharmawan, Ruben; Darukutni, Darukutni; Haryati, Sri; Murkati, Murkati; Sari, Yulia; Prasetyo, Afiono Agung
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol 19, No 3 (2013)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v19i3.419

Abstract

Candida albicans β-D-glucans examination has been used for invasive fungal detection in human blood and was approved by the Food and Drug Administration. However, the method is rarely used in Indonesia and also because the cost is hardly affordable. A modification of the method using enzymatic reaction is hoped will provide a simple and affordable measurement in human blood serum. β-D-glucans as heterogeneous molecules constitute the major carbohydrates fractions of cell wall and readily detected in supernatants of Candida albicans cultures are hydrolyzed by β glucanase to form D-glucose. This additional glucose is measured using Megazyme GOPOD-Format Procedure® at 510 nm. Candida albicans were identified and cultured was derived from a patient of Dr. Moewardi General Hospital, Surakarta in July, 2012. The results show that β-D-glucans from Candida albicans is measureable to the amount of μg/100 μL serum using this modification principle
DETEKSI VIRUS DEN3 PADA SEMUA STADIUM Aedes aegypti (TRANSOVARIAL) DENGAN TEKNIK IMUNOSITOKIMIA SECARA INVITRO Kumoro Setya, Adhi; Dharmawan, Ruben
Jurnal Farmasi (Journal of Pharmacy) Vol. 5 No. 1 (2016): Jurnal Farmasi (Journal of Pharmacy), October 2016
Publisher : Jurnal Farmasi (Journal of Pharmacy)

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Abstract

Many dangerous parasites transmitted by the mosquito, one of which is dengue virus which causes dengue fever. The previous study said that in immunocytochemistry, dengue virus can be found in A. aegypti that does not suck blood. The basic principle of this examination was done by a technique of determining the existence (location) of antigen in cells in a tissue using antigen-antibody reaction. The purpose of this study was to determine the result of positive value of A. aegypti breeding stages in laboratory scale. DEN3 virus was mixed into the blood for trial then infected orally on the A. aegypti for breeding. All stages of breeding results was tested for DEN3 virus from the body tissue. DEN3 antigen in the body of the vector will bind with specific antibody from reagent and by the presence of the enzyme and chromogen substrate, it will give a brown color to the cells and granule surrounding the cells. Such color reaction was checked using a light microscope with 400x magnification with the positive control of DEN3 breed and the negative control of normal blood as a comparison. The findings showed that immunocytochemistry with SBPC method was capable of detecting DEN3 at all stages of metamorphosis of A. aegypti with the results of the stage respectively; egg: 82%, larva: 89%, pupa: 94% and adult: 100%. These results showed how immunocytochemistry technique as an alternative method which is relatively cheaper than molecular technique can be used for surveillance on dengue will appear.
Co-Authors -, Muryanti . Soetrisno . Suhanantyo Adi Prayitno Afiono Agung Prasetyo Aris Sudiyanto Asri Sukawati Putri Atika Puspita Hapsari Atma Sanggani Tunikasari Bhisma Murti Darukutni, Darukutni Dyah Kusumawati Dyah Mustikaningtyas Dewanti I Gusti Bagus Wiksuana Icha Dithyana Irene Yunita Prihandini Kharisma Setya Harnani Kumoro Setya, Adhi Mardiatmi Susilohati Mashuri, Yusuf Ari Mira Rizki Ramadhan, Mira Rizki Murkati, Murkati Narzah, Norma Arista Pawito -, Pawito Sofi Ariani Sri Haryati Tamaulina Br Sembiring yoseph Indrayanto