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All Journal Media Veteriner Jurnal Veteriner Jurnal Ilmu Ternak Veteriner WARTAZOA Indonesian Bulletin of Animal and Veterinary Sciences Jurnal Kedokteran Hewan

Indrawati Sendow

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Author-ID : 2541837

Biochemistry, Genetics & Molecular Biology Immunology & microbiology Medicine & Pharmacology Veterinary

Published : 21 Documents Claim Missing Document

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Articles

Title

Pathogenicity study of local bluetongue virus isolates in local and imported sheep Indrawati Sendow
Jurnal Ilmu Ternak dan Veteriner Vol 10, No 1 (2005): MARCH 2005
Publisher : Indonesian Center for Animal Research and Development (ICARD)

Bluetongue is one of arboviruses that caused economical impact to sheep farmers. Six local bluetongue virus (BT) serotypes isolates were obtained from sentinel cattle blood in West Java and Irian Jaya (Papua), but its pathogenicity has not been identified. Propagation of viraemic blood inoculum from 3 local BT serotypes such as serotypes 1,9 and 21, that had been conducted in Merino sheep, will be used for pathogenicity study. The study was devided into 3 groups, each group contained local and imported sheep as control and infected sheep. All sheep had been tested as negative BT antibodies. Observation on clinical signs had been conducted twice daily for 28 days. Heparinised blood and sera were collected everyday to obtain the viraemia period by Ag-C-ELISA test and antibody respons by C-ELISA test. The clinical signs produced were varied from normal to very mild in local sheep and very mild to mild-moderate in Merino sheep.The lowest severe degree of clinical signs was BT 9 followed by BT 1 and BT 21. No dead, neither local and Merino sheep occurred. Viraemia in Merino sheep occurred between 3-5 days and in local sheep between 4-7 days post inoculation (DPI). Antibody respons occurred as quick as 10 DPI in Merino sheep and 9 DPI in local sheep, and stayed until the end of experiment. This study showed that local BT isolates were not pathogen and not producing clasical BT infection. Key Words: Bluetongue Virus, Antibody Respons, Viraemia, Serology, Sheep

Gambaran seroepidemiologi dan histopatologi infeksi virus parainfluenza tipe 3 pada sapi Indrawati Sendow; Tatty Syafriati; Rini Damayanti
Jurnal Ilmu Ternak dan Veteriner Vol 9, No 2 (2004): JUNE 2004
Publisher : Indonesian Center for Animal Research and Development (ICARD)

A study to gain seroepidemiological feature and histopathological changes in order to obtain a viral causative agent had been conducted against parainfluenza virus type 3 (PI-3) in infection cattle in Indonesia. Serological survey was conducted in different areas in Indonesia and from serum Bank to gain the information on the distribution of parainfluenza type 3 (PI-3) in large ruminants. A total of 1334 sera had been tested using serum neutralization test, and the result indicated that prevalence of reactors was varied from 0 to 60 %. The highest prevalence was 60% in sera detected from Bogor abbatoir. Reactors were also found in other areas such as West Java, Central Java, East Java, NTT and Papua. Titration results indicated that the distribution of titre was varied from 4 to 256, and titre of 8 to 32 was the most common. Titre of 128 and 256 was only found in each of 1 sera only. Isolation results indicated that no isolate was obtained from 237 samples processed. Histological examination showed that more than 60% had interstitial pneumonia, which indicated vairal infection had been occurred. This serological result indicated that PI-3 infection was detected in Indonesian large ruminants. Key words: Parainfluenza type 3 virus, serology, histopathology

Antigen-capture enzyme-linked immunosorbent assay using monoclonal antibody for detection of bluetongue virus antigen An Indrawati Sendow
Jurnal Ilmu Ternak dan Veteriner Vol 2, No 4 (1998)
Publisher : Indonesian Center for Animal Research and Development (ICARD)

antigen-capture enzyme-linked immunosorbent assay (ELISA) using a specific anti-bluetongue virus group was applied to detect bluetongue viral antigen . The test was specific for bluetongue viruses and did not detect the closely related epizootic haemorrhagic disease of deer viruses (EHDV) and other orbiviruses . It was easy to perform and could be established in laboratories which have simple facilities . The antigen-capture ELISA technique is an alternative method to agar gel immunodiffusion and immuno-dot blotting tests to detect bluetongue antigen in infected tissues, Vero cells, Aedes albopictus cells and BHK-21 cell cultures . Keywords : ELISA, monoclonal antibody, antigen detection, BT

Isolation of bluetongue serotypes 1, 6 and 21 from insects in West Java indrawati sendow
Jurnal Ilmu Ternak dan Veteriner Vol 7, No 4 (2002): DECEMBER 2002
Publisher : Indonesian Center for Animal Research and Development (ICARD)

Bluetongue virus is one of arbovirus diseases which is transmitted by insects, Culicoides spp. Insect collection wasconducted weekly from 1991 to 1993 and forthnightly from 1993 to 1997 in West Java using Pirbright-type miniature light trapin to phosphate buffered saline for identification and viral isolation. A total of 1155 pools of insects were inoculated intoembryonated chicken eggs before passaging in a mosquito cell line and three times blind passages in BHK-21 cells. Fourteenpools of insects produced cytopathic effect in BHK-21 cells. Four of the infected BHK-21 cells reacted in the antigen captureELISA test using a specific monoclonal antibody to bluetongue (BTV) virus. Further identification into serotype in ReferenceLaboratory, indicated that BTV serotype 1 was isolated from C. fulvus, BTV serotype 6 was isolated from C. peregrinus andBTV serotype 21 from pools of C. shortii and C. orientalis.Key words: Bluetongue viruses, isolation, insects

Serological study against transmissible gastroenteritis (TGE) virus in several area in Indonesia Indrawati Sendow; Tatty Syafriati; Sjamsul Bahri; Antonius Sarosa
Jurnal Ilmu Ternak dan Veteriner Vol 3, No 3 (1998)
Publisher : Indonesian Center for Animal Research and Development (ICARD)

A number of 1,168 pig and dog sera from 8 provinces in Indonesia were tested serologically for transmissible gastroenteritis (TGE) antibodies using serum neutralisation test to detect the prevalence of TGE in Indonesia. The sera were obtained from serum bank at Research Institute for Veterinary Science, Bogor. All sera collected before 1995 were negative antibody to TGE. However, sera collected from 2 provinces Sumatera Utara and Sulawesi Utara in 1996 had antibodies against TGE virus (14.03%). Titration of reacted sera showed varied between titres of 8 to 128. Key words: Transmissible gastroenteritis virus, serum neutralization test

The isolation of canine parvovirus and pathological changes of infected dogs Indrawati Sendow; Helmy Hamid
Jurnal Ilmu Ternak dan Veteriner Vol 9, No 1 (2004): MARCH 2004
Publisher : Indonesian Center for Animal Research and Development (ICARD)

The aims of this study are to isolate canine parvovirus (CPV) from the field case and to evaluate its histological aspects in CPV infected dogs. Samples were collected from intestine, intestine contained and mucose, as well as dogs feses from diarrhoea and blood diarrhoea. The suspension of those specimen was inoculated into Feline Kidney cells and observed for cythopathic effect (CPE). The presence of CPE indicated that there was viral isolate and continued to further identification using Haemaglutination (HA) test with pig red blood cells. Samples with positif in HA test were further identification using Haemaglutination Inhibition (HI) test against reference CPV antisera. Isolation result showed that from 81 samples processed, 10 samples indicated CPE in cell cultures, and had agglutinated in pig red blood cells and neutralised reference CPV antisera as the same titer of reference CPV antisera. Nine isolates were obtained from feces and 1 isolate was obtained from Mucose intestine from bloody diarrhoea dogs. Those isolates were also obtained from 1 to 2 days post blood diarrhea clinical signs. Two from 10 cadavers examined showed histological changes to CPV infection. Isolate of CPV, originally from feces, was also obtained from one of the two cadavers. Based on the results it was concluded that more CPV viral isolates can be obtained from bloody diarrhea feces. Key words: Canine parvovirus, inhibition, isolation, pathology, identification

The detection antibody of porcine reproductive and respiratory syndrome (PRRS) in pig sera in Eastern part of Indonesia Indrawati Sendow; Sjamsul Bahri; Antonious Sarosa
Jurnal Ilmu Ternak dan Veteriner Vol 2, No 3 (1997)
Publisher : Indonesian Center for Animal Research and Development (ICARD)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14334/jitv.v2i3.68

Since the early 1980's, a new and highly contagious disease syndrome of pigs, porcine reproductive and respiratory syndrome (PRRS), has been spreading through major pig producing areas in the world . It is suspected that the disease has occurred in Indonesia.To confirm the presence of PRRS infection in Indonesia, a serological survey in pig was conducted between 1993 and 1995 in some areas in Eastern Indonesia to detect antibodies against PRRS virus using ELISA test . The results indicated that a total of 822 pig sera were tested and 8% of these sera had antibodies against PRRS virus . Pig sera which were collected from slaughter house in Jakarta showing the highest prevalence (30%) of reactor. However, there was no antibody was detected from pig sera originated from Irian Jaya since 1993 . Key words: PRRS, prevalence, ELISA test, antibody

Infection of Parainfluenza type 3 (PI-3) as one of the causative agent of pneumonia in sheep and goats Indrawati Sendow; Tatty Syafriati; Ening Wiedosari; Paul Selleck
Jurnal Ilmu Ternak dan Veteriner Vol 7, No 1 (2002): MARCH 2002
Publisher : Indonesian Center for Animal Research and Development (ICARD)

Serological survey was conducted to obtain the prevalence of Parainfluenza type 3 (PI-3) reactor as one of the causative agent of pneumonia in sheep and goats in abatoir at Jakarta and some small holder farms in Indonesia. Serological test using serum neutralization from 724 goat sera and 109 sheep sera indicated that only 1% of goats were serologically reactors and none of sheep sera had antibodies against PI-3 virus. Isolation of the virus from 56 bronchus and trachea swab and 345 lungs indicated that only one sampel from lung showed cythopathic effect (CPE) in Madin Darby Bovine Kidney (MDBK) cell lines identification of the virus using serum neutralization test indicated that the virus neutralized reference PI-3 antisera. The isolate came from one lung (7%) of 24 that showed histopathologically pneumonia intertitialis that usually caused by viral infection. Key words: Parainfluenza type 3 (PI-3), virus isolation, sheep, goat, pneumonia intertisialis

Epidemiology of Japanese–B– encephalitis infection in pigs in Riau and North Sumatera Provinces Indrawati Sendow; Tatty Syafriati; Upik Kesumawati Hadi; Martin Malole; Susi Soviana; Darminto .
Jurnal Ilmu Ternak dan Veteriner Vol 8, No 1 (2003): MARCH 2003
Publisher : Indonesian Center for Animal Research and Development (ICARD)

Epidemiology study on Japanese-B-Encephalitis (JE) was conducted in Riau and North Sumatera Provinces. A total of 190 pig sera from Riau Province and 164 pig sera from North Sumatera were tested using competitive ELISA (C-ELISA) to detect antibodies against JE virus. Insect collection was also conducted using several methods near pig farms in those provinces and identified into species to gain more information on its role to distribute JE infection. Serological results indicated that 70% pig in Sumatera and 94% pig in Riau had antibodies against JE virus. The highest prevalence of reaktor was detected in pig of more than 4 months age in both Provinces. The results of insect collection showed that Culex tritaeniorchynchus and Culex quinquefasciatus were the most dominant species in both provinces. Based on serological testing, indicated that JE virus infected pig in Sumatera and Riau Provinces, and higher reactor was obtained in older pig. Culex tritaeniorchynchus and Culex quinquefasciatus were the dominant insect species in both provinces, hence those species had a possibility to play an important role of JE transmission. Key words: JE, pigs, serology, insects

Seroepidemiology of Canine parvovirus infection in dogs Indrawati Sendow; Tatty Syafriati
Jurnal Ilmu Ternak dan Veteriner Vol 9, No 3 (2004): SEPTEMBER 2004
Publisher : Indonesian Center for Animal Research and Development (ICARD)

Canine parvovirus is an acute and fatal viral disease in dogs. A total of 209 local, cross breed and breed dogs sera from Kodya Bogor, Kabupaten Bogor, Sukabumi, and Jakarta, had been tested using Haemagglutination Inhibition Test (HI) with pig red blood cells. A total of 64 breed and cross breed dogs from Sukabumi and Kodya Bogor, were used as a sentinel dogs to study the epidemiology of Canine parvovirus (CPV) infection and its immunological responses caused by vaccination. The results indicated that 78% (95) breed and cross bred dogs and 59% (51) local dogs had antibody to CPV. Sentinel dogs results indicated that dogs had been vaccinated showed antibody response with the varied titre dependant upon prevaccination titre. Low prevaccinated titre gave better response than protective level titre. From 19 puppies observed, Maternal antibodi were still detected until 5 weeks old puppies. First vaccination given at less than 3 months old, should be boosted after 3 months old puppied. Antibodi titre produced by natural infection will keep untill 2 years. These data concluded that the dog condition and time of vaccination will affect the optimum antibody response. Key words: Canine parvovirus, haemagglutination inhibition, isolation

Co-Authors Agik Suprayogi Antonious Sarosa Antonius Sarosa Atik Ratnawati Atik Ratnawati Darminto . Ening Wiedosari Helmy Hamid I Gusti Agung Ayu Suartini I Gusti Ngurah Kade Mahardika I wayan Teguh Wibawan M Saepullah Martin Malole Muharam Saepullah Muharam Saepulloh Ni Luh Putu Agustini NLP Indi Dharmayanti NLPI Dharmayanti Nur Sabiq Assadah Paul Selleck R M Abdul Adjid R.M. Abdul Adjid RMA Adjid Sembiring, Rinawati Sjamsul Bahri Susi Soviana Tatty Syafriati Tri Budhi Murdiati Upik Kesumawati Hadi

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