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Penetapan Kadar Flavonoid Total Ekstrak Etanol 70% Daun Bayam Brazil (Althernanthera sisso) Dengan Metode Spektrofotometri UV-Vis Nur Humaira Marwati; Revita Saputri; Eka Fitri Susiani
BORNEO JOURNAL OF PHARMASCIENTECH Vol 8 No 1 (2024): Borneo Journal of Pharmascientech
Publisher : Universitas Borneo Lestari

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.51817/bjp.v8i1.496

Abstract

Brazilian Spinach (Althernanthera sisso) leaves are known to have a high natural antioxidant activity content of 3.7 ppm. Antioxidants have an important role in the human body to prevent damage caused by free radicals. One of the compounds that have antioxidant activity is flavonoids. Based on this description, it is necessary to know the content of total flavonoids in A. sisso This study aims to determine qualitatively using Thin Layer Chromatography (TLC), while quantitatively using UV-Vis spectrophotometry. Maceration method with 70% ethanol solvent was used to extract Brazilian spinach leaves. Thin Layer Chromatography (TLC) followed by spray reagents was used to test the flavonoid compounds. Quercetin reagent was used to determine total flavonoid levels, while AlCl3 was used to determine total flavonoid levels. The results of this qualitative test showed the presence of flavonoids, and in the quantitative test using UV-Vis spectrophotometry the total levels of flavonoids were 70.890 ± 1.723% w/w quercetin equivalent. The 70% ethanol extract of A. sisso leaves contains flavonoids so that it has the potential to be developed as a medicinal plant
PERBANDINGAN METODE EKSTRAKSI MASERASI DAN SOKHLETASI TERHADAP KADAR FLAVONOID TOTAL EKSTRAK ETANOL 96% DAUN KLUWIH (Artocarpus camansi) Regita Wananda Putri Gita; M. Hidayatullah; Eka Fitri Susiani
Jurnal Ilmiah Manuntung: Sains Farmasi Dan Kesehatan Vol. 10 No. 2 (2024): Jurnal Ilmiah Manuntung: Sains Farmasi Dan Kesehatan
Publisher : Sekolah Tinggi Ilmu Kesehatan Samarinda

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.51352/jim.v10i2.840

Abstract

The utilization of medicinal plants is one of the steps for alternative medicine. One of them is the kluwih plant (Artocarpus camansi) which is also similar to the breadfruit plant but has differences in its fruit. The purpose of this research is to analyze the total flavonoid content of 96% ethanol extract of kluwih leaves and which method has the potential to produce greater total flavonoid content. Extraction of kluwih leaves was done by maceration and sokhletation. The method implemented was UV-Vis spectrophotometry with AlCl3 reagent and involving quercetin as a comparator. Based on the analysis by applying the maceration method, an average level of 23.05 mg QE/g extract was obtained for the total flavonoid content of 96% ethanol extract of kluwih leaves, while the sokhletation method obtained an average of 31.33 mg QE/g extract. So it can be concluded that the extraction method that produces a greater total flavonoid content of 96% ethanol extract of kluwih leaves is sokhletasi.