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All Journal JURNAL FARMASI DAN ILMU KEFARMASIAN INDONESIA Jurnal Pengabdian Al-Ikhlas Borneo Journal Of Pharmascientech Jurnal Insan Farmasi Indonesia Jurnal Ilmiah Ibnu Sina (JIIS): Ilmu Farmasi dan Kesehatan Borneo Journal of Pharmacy Jurnal Ilmiah Manuntung: Sains Farmasi dan Kesehatan Journal of Pharmascience
Eka Fitri Susiani
Sekolah Tinggi Ilmu Kesehatan Borneo Lestari
Biochemistry, Genetics & Molecular Biology Chemistry Economics, Econometrics & Finance Education Engineering Health Professions Immunology & microbiology Medicine & Pharmacology Nursing Physics Public Health Social Sciences Other

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Penetapan Kadar Flavonoid Total Ekstrak Etanol 70% Daun Bayam Brazil (Althernanthera sisso) Dengan Metode Spektrofotometri UV-Vis Nur Humaira Marwati; Revita Saputri; Eka Fitri Susiani
BORNEO JOURNAL OF PHARMASCIENTECH Vol 8 No 1 (2024): Borneo Journal of Pharmascientech
Publisher : Universitas Borneo Lestari

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.51817/bjp.v8i1.496

Abstract

Brazilian Spinach (Althernanthera sisso) leaves are known to have a high natural antioxidant activity content of 3.7 ppm. Antioxidants have an important role in the human body to prevent damage caused by free radicals. One of the compounds that have antioxidant activity is flavonoids. Based on this description, it is necessary to know the content of total flavonoids in A. sisso This study aims to determine qualitatively using Thin Layer Chromatography (TLC), while quantitatively using UV-Vis spectrophotometry. Maceration method with 70% ethanol solvent was used to extract Brazilian spinach leaves. Thin Layer Chromatography (TLC) followed by spray reagents was used to test the flavonoid compounds. Quercetin reagent was used to determine total flavonoid levels, while AlCl3 was used to determine total flavonoid levels. The results of this qualitative test showed the presence of flavonoids, and in the quantitative test using UV-Vis spectrophotometry the total levels of flavonoids were 70.890 ± 1.723% w/w quercetin equivalent. The 70% ethanol extract of A. sisso leaves contains flavonoids so that it has the potential to be developed as a medicinal plant
PERBANDINGAN METODE EKSTRAKSI MASERASI DAN SOKHLETASI TERHADAP KADAR FLAVONOID TOTAL EKSTRAK ETANOL 96% DAUN KLUWIH (Artocarpus camansi) Regita Wananda Putri Gita; M. Hidayatullah; Eka Fitri Susiani
Jurnal Ilmiah Manuntung: Sains Farmasi Dan Kesehatan Vol. 10 No. 2 (2024): Jurnal Ilmiah Manuntung: Sains Farmasi Dan Kesehatan
Publisher : Sekolah Tinggi Ilmu Kesehatan Samarinda

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.51352/jim.v10i2.840

Abstract

The utilization of medicinal plants is one of the steps for alternative medicine. One of them is the kluwih plant (Artocarpus camansi) which is also similar to the breadfruit plant but has differences in its fruit. The purpose of this research is to analyze the total flavonoid content of 96% ethanol extract of kluwih leaves and which method has the potential to produce greater total flavonoid content. Extraction of kluwih leaves was done by maceration and sokhletation. The method implemented was UV-Vis spectrophotometry with AlCl3 reagent and involving quercetin as a comparator. Based on the analysis by applying the maceration method, an average level of 23.05 mg QE/g extract was obtained for the total flavonoid content of 96% ethanol extract of kluwih leaves, while the sokhletation method obtained an average of 31.33 mg QE/g extract. So it can be concluded that the extraction method that produces a greater total flavonoid content of 96% ethanol extract of kluwih leaves is sokhletasi.
Evaluation of Extraction Methods on the Antioxidant Potential of Balik Angin Leaves Ethanol Extract (Alphitonia incana (Roxb.) Teijsm. & Binn. ex Kurz) Putri Indah Sayakti; Hafiz Ramadhan; Dyera Forestryana; Eka Fitri Susiani; Nadya Aprillinia; Nadhia Khairany; Wardah Izzatul Awwaliyah; Winda Ameilia Dewi; Nur Syifa
Journal of Pharmascience Vol. 13 No. 1 (2026): Jurnal Pharmascience
Publisher : Universitas Lambung Mangkurat

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20527/jps.v13i1.24662

Abstract

Alphitonia incana, also known locally as balik angin, is an endemic natural antioxidant plant of Borneo. The objective of the present work was to compare the effectiveness of maceration and Soxhlet extraction of balik angin leaves with respect to antioxidant activity and phenolic–flavonoid content. Ethanolic extracts were prepared using both extraction methods, and the phenolic-flavonoid content was quantified spectrophotometrically. Antioxidant performance was assessed through DPPH, CUPRAC, and FRAP assays. The findings revealed that the Soxhlet-derived extract contained higher concentrations of phenolic-flavonoid compounds than those produced by maceration. In addition, the Soxhlet-derived extract demonstrated stronger antioxidant activity, as evidenced by lower IC₅₀ and EC₅₀ values in the DPPH (10.872 ppm) and CUPRAC (6.578 ppm) assays compared with the macerated extract (IC₅₀ 13.127 ppm; EC₅₀ 14.034 ppm) and was classified as a very strong antioxidant. The FRAP assay further supported these results, with ferric reducing power values of 1,484.1 mg AAE/g extract for the Soxhlet-derived extract and 1,295.8 mg AAE/g extract for the macerated sample. Overall, the study concludes that Soxhlet extraction is more effective than maceration in enhancing the antioxidant potential of balik angin leaf, a result that is closely associated with their elevated phenolic and flavonoid content.
Co-Authors Any Guntarti Astuti, Karunita Ika Azmi Yunarti Dea Permata Rezky Dyera Forestryana Guntur Kurniawan Hafiz Ramadhan Hafiz Ramadhan I Ketut Adnyana Khairina Maida Kintoko, Kintoko Lusiana Dwi Wulandari M. Hidayatullah Muhammad Reza Fahlevi Muhammad Reza Pahlevi Nadhia Khairany Nadya Aprillinia Nur Humaira Marwati Nur Syifa Pratiwi Wikaningtyas Putri Indah Sayakti Rahmatullah, Satrio Wibowo Regita Wananda Putri Gita Risti Hadiyanti Sandi, Dita Ayulia Dwi Saputri, Revita Siti Nurhaliza Leyla Wardah Izzatul Awwaliyah Winda Ameilia Dewi