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PEMERIKSAAN MIKROSKOPIS KUALITAS SEDIAAN TELUR CACING Trichuris trichiura MENGGUNAKAN METODE NATIF DAN METODE FLOTASI Nurhidayanti; Indah Sari
jurnal kesehatan terapan sains dan teknologi Vol 1 No 2 (2023): Journal Health Applied Science And Technology (JHAST)
Publisher : IKesT Muhammadiyah Palembang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52523/jhast.v1i2.16

Abstract

Background: Cases of helminthiasis in children are more often infected by Soil Transmitted Helmint (STH) worms, one of which is Trichuris trichiura (whipworm). Stool examination aims to determine the presence or absence of worm eggs or infective larvae. Examination of worm eggs by native and flotation methods is a qualitative examination, each of which has advantages and disadvantages. In the native method, the process is fast, but less sensitive in mild infections. The flotation method has the advantage that the field of view of the microscope is free from faeces so that it is easier to read on a microscope. The drawbacks of the flotation examination are that it takes quite a long time and is more suitable for use in feces that contain small amounts of worm eggs. Purpose: to see the quality of Trichuris trichiura worm egg preparations native and flotation methods. Research Methods: This method is used as an alternative because the native method still has drawbacks, especially in mild infections. This type of research is descriptive analysis. The research was conducted in the Hematology Laboratory of the Muhammadiyah Palembang Institute of Health Sciences and Technology on January 3, 2022 with a total sample of 32 preparations. Results: the study found that the flotation method was more optimal in detecting Trichuris trichiura worm eggs than the native method, with very good quality assessment results on the flotation method 10 preparations of 16 preparations (62.5%), while the native method obtained very good results for 3 preparations of 16 preparations (18.8%). Conclusion: from the research results, there are differences in the results between the flotation method and the native method.
EDUKASI FLEBOTOMI PADA SISWA SMK INDO HEALTH SCHOOL PALEMBANG JURUSAN TEKNOLOGI LABORATORIUM MEDIS Indah Sari
Khidmah Vol 3 No 2 (2021): Khidmah
Publisher : IKesT Muhammadiyah Palembang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52523/khidmah.v3i2.349

Abstract

A clinical laboratory is a health laboratory that carries out clinical specimen examination services to obtain information about individual health, especially to support efforts to diagnose disease, cure disease, and restore health. The results of laboratory tests are strongly influenced by the pre-analytical, analytical and post-analytic stages. The biggest error contribution in the laboratory, namely in the pre-analytic stage, occurred at 77.1%. One of the health services at the forefront of laboratory services is phlebotomy. Phlebotomy is one of the main reasons behind pre analytic errors. Currently medical laboratory technology experts pay less attention to pre-analytical processes in the laboratory such as the process of taking blood samples (phlebotomy) so it is necessary to socialize and educate about phlebotomy for students of SMK Indo Health School (IHS) majoring in medical laboratory technology which aims to increase knowledge and understanding students regarding the pre-analytic stage, especially in blood sampling (phlebotomy) so as to prevent errors in the post-analytic stage. Solutions that can answer the problems faced by medical laboratory technology experts, it is necessary to carry out socialization and education about phlebotomy, so as to increase the knowledge and understanding of students of SMK Indo Health School (IHS) majoring in medical laboratory technology, totaling 30 participants at the pre-analytic stage, especially in taking blood samples (phlebotomy) as well as the selection of poster education media aims to explain about phlebotomy, demonstrate the stages of phlebotomy and the importance of a good phlebotomy stage in laboratory examination.
Differences in Iron (Fe) Content in Samples of Refill Drinking Water (AMIU) and Bottled Drinking Water (AMDK) Atomic Absorption Spectrophotometry Method Indah Sari; Rossa Veronneca; Anita
Jurnal Inspirasi Kesehatan Vol. 1 No. 2 (2023): Spesial Edisi Khusus (Proceeding)
Publisher : Fakultas Kesehatan IKesT Muhammadiyah Palembang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52523/jika.v1i2.54

Abstract

Introduction: Iron is a metal in the first transition Series and the most common element on Earth by mass that makes up most of the outer and inner core of the Earth. High levels of iron (Fe) can be polluted by corrosive gases such as CO2 and H2S. In nature there are various elements that include heavy metals and also a component of pollutants, namely Cd, Hg, Cr, Pb, Ni, Cu, Zn, and Fe. The Aim Of The Research: To determine the difference in iron content in samples of drinking water refills and bottled drinking water Atomic Absorption spectrophotometric method. Research Method: This research was conducted at the mathematics and Natural Sciences Laboratory of Sriwijaya University in January-February 2022 with a total of 20 samples. The Result: The results of the study obtained the average value of iron (Fe) content in refilled drinking water samples 7.7 mg/L and bottled drinking water 3.7 mg/L Atomic Absorption Spectrophotometry method. Mann Witney test obtained the value of p (Sig 2 Tailed) = 0.04. Conclussions: Have different levels of iron in samples of drinking water refills and bottled drinking water Atomic Absorption spectrophotometric method.
Edukasi tentang Stabilitas Ekstrak Buah Parijoto sebagai Counterstain pada Pewarnaan Gram Berdasarkan Variasi Suhu Indah Sari
Khidmah Vol 8 No 1 (2026): Khidmah
Publisher : IKesT Muhammadiyah Palembang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52523/khidmah.v8i1.570

Abstract

Gram staining is a differential staining procedure that divides bacteria into Gram-positive and Gram-negative groups. Safranin is one of the dyes used in Gram staining. The toxicity and health hazards posed by the use of safranin dye have been widely reported, particularly the dangers of dye contamination originating from synthetic dye waste. The use of synthetic dyes is very dangerous because it can trigger cancer, kidney and liver damage. Anthocyanins are natural pigments that have the potential to be used as dyes, including in laboratory applications such as Gram staining. Gram staining is an important technique in bacterial classification, which usually uses synthetic dyes such as safranin to detect Gram-negative bacteria. However, the use of such synthetic dyes can have adverse environmental impacts, so alternative natural dyes that are more environmentally friendly are needed. Parijoto fruit (Medinilla speciosa) is red and contains anthocyanins. Anthocyanins have low stability to the effects of heat. The use of natural dyes can reduce synthetic dye waste in bacterial staining. However, natural dyes are susceptible to temperature. The method used is education using learning posters as well as pre- and post-tests. The main focus of the activity is to increase understanding and knowledge. The participants of the activity were 6 laboratory staff of Ar-Rasyid Islamic Hospital, Palembang, who had received the education. The evaluation results of the pre-test and post-test scores of the educational activity showed an increase, which means that the laboratory staff of Ar-Rasyid Islamic Hospital, Palembang, had a better understanding and increased knowledge regarding the education on the Stability of Parijoto Fruit Extract as a Counterstain in Gram Staining Based on Temperature Variations.
Perbedaan Kadar Trigliserida Menggunakan Serum dan Plasma Edta dengan Biosystem A15 Sarah Aminah; Indah Sari; Bastian Bastian
Jaringan Laboratorium Medis Vol 4 No 1 (2022): Mei 2022
Publisher : Poltekkes Kemenkes Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31983/jlm.v4i1.8402

Abstract

Trigycerides are the main lipid component in food intake, which is about 98% of total lipids and the remaining 2% consists of phospholipids and cholesterol. Triglycerides are present in adipose tissue, skeletal muscle, liver, lungs, and intestines to  provide energy in metabolic processes. Who estimates about 1 bilion individuals are overweight and about 300 milion individuals are defined as obese, normal triglyceride levels usually do not exceed 150 mg/dl, if triglyceride levels increase by more than 200 mg/dl then called hypertriglyceridemia. The research aims to determine the difference in triglceride levels using serum and plasma EDTA conducted at the Center For Health Laboratories (BBLK) Palembang. The type of research used is cross Sectional with research designintact group comparison. The sample consisted of 30 serums and 30 plasma EDTA taken from 30 respondents. Research is carried out starting from the preparation of patients, taking examination materials, processing of examination materials, analysis and results of research. The average levels of triglyceride examination using serum and plasma were obtained consecutively at 0.91 mmol/L and 0.83 mmol/L. Mann Whitney test results found that the significant value was p = 0.240. The p value obtained is p>0.05. The results can be concluded that there is no difference in examination of triglyceride levels using serum and plasma EDTA.