Article Per Year (5 Year)
p-Index From 2020 - 2025
1.048
P-Index
This Author published in this journals
All Journal AL KAUNIYAH Quagga Biology, Medicine, & Natural Product Chemistry Bioeksperimen: Jurnal Penelitian Biologi Jurnal Florea JITEK (Jurnal Ilmiah Teknosains) Jurnal Surya Masyarakat Jurnal Ilmu Kefarmasian Indonesia Jurnal Surya Masyarakat
Dyah Ayu Widyastuti
Universitas PGRI Semarang
Religion Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Civil Engineering, Building, Construction & Architecture Computer Science & IT Education Engineering Environmental Science Health Professions Materials Science & Nanotechnology Mechanical Engineering Medicine & Pharmacology Public Health Veterinary Other

Published : 10 Documents Claim Missing Document
Claim Missing Document
Check
Articles

Found 10 Documents
Search

 1 
Antioxidant Capacity Comparison of Ethanolic Extract of Soursop (Annona muricata Linn.) Leaves and Seeds as Cancer Prevention Candidate Dyah Ayu Widyastuti; Praptining Rahayu
Biology, Medicine, & Natural Product Chemistry Vol 6, No 1 (2017)
Publisher : Sunan Kalijaga State Islamic University & Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (307.566 KB) | DOI: 10.14421/biomedich.2017.61.1-4

Abstract

Annona muricata Linn. (soursop) is one of  tropical plants which have relatively complete chemical compounds. It has flavonoid, tannin, phytosterol, alkaloid, etc. The high antioxidant compound in soursop is believed as cancer prevention so the cancer threat in the world can be minimized. The antioxidant compound in soursop can be found not only in its fruit, but also in other parts like leaves, seeds, etc. Based on that potency, this study aimed to compare antioxidant capacity of soursop leaves and seeds, also to study about the utilization of soursop parts which is usually not used. This research began with maceration to extract leaves and seeds with 96% ethanol. Ethanolic extract of soursop leaves and seeds were then tested for antioxidant capacity with DPPH (1,1-diphenyl-2-picrylhydrazyl) method. The result showed that antioxidant capacity of soursop leaves and seeds are 85,66875% and 39,0166, respectively. The antioxidant capacity of leaves is higher than seeds due to seed’s extraction difficulty so its antioxidant compound could not be extracted optimally. However, either leaves or seeds extract in this study are potential as antioxidant resources because there are no significant differences between antioxidant capacity of both extract.
TERAPI GEN: DARI BIOTEKNOLOGI UNTUK KESEHATAN Dyah Ayu Widyastuti
Al-Kauniyah: Jurnal Biologi Vol 10, No 1 (2017): Al-Kauniyah Jurnal Biologi
Publisher : Department of Biology, Faculty of Science and Technology, Syarif Hidayatullah State Islami

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (630.513 KB) | DOI: 10.15408/kauniyah.v10i1.4864

Abstract

Abstrak Rekayasa genetika dilakukannya untuk manipulasi gen-gen tertentu sehingga ekspresi gen dapat dikontrol dan produknya dapat dimanfaatkan untuk tujuan tertentu, salah satunya untuk pengobatan penyakit dengan terapi gen. Terapi ini melibatkan transfer asam nukleat berupa DNA ke sel embrionik maupun somatik pasien sehingga gen tersebut memiliki efek pengobatan terhadap penyakit pasien. Gen fungsional yang ditransfer berperan menggantikan fungsi gen abnormal yang menyebabkan penyakit tertentu. Terapi gen dapat dilakukan pada sel embrional (germ line gene therapy) maupun sel somatik (somatic cells gene therapy) pada pasien secara in vivo maupun ex vivo. Penyisipan gen pada terapi ini menggunakan vektor virus maupun non virus. Keberhasilan terapi gen salah satunya tergantung pada efektifitas transfer gen yang dilakukan dan ekspresi gen fungsional. Gen fungsional yang telah tertransfer selanjutnya harus diekspresikan dengan baik sehingga terapi dapat berhasil. Kanker merupakan salah satu penyakit yang memungkinkan dilakukannya terapi gen, terkait dengan adanya abnormalitas gen penyebab proliferasi sel yang tidak terkontrol. Abstract Genetic engineering has the ability to manipulate specific genes so its expression can be controlled. The control of gene expression can be used as a gene functional for appropriate purposes, such as diseases cure with gene therapy. This therapy involves DNA as nucleic acid which is transfer to embryonic or somatic cells of patients with certain diseases. The purpose of the gene therapy is to switch an abnormal gene to be a functional gene which has a cure effect for the disease. The functional gene has to substitute the abnormal gene leads to certain disease, either germ line gene therapy or somatic cells therapy. The gene therapy needs a vector to carry the functional gene to targeted cells, in vivo or ex vivo. The vector can be used viral or non viral vectors. The possibilities of achievement of its therapy depend on gene transfer effectiveness and also functional gene expression. The cancer is the one of diseases which can be treated with the gene therapy due to its uncontrolled gene of cell proliferation.
Konsentrasi Kreatinin dan Urea dalam Darah Tikus Putih (Rattus norvegicus) Galur Wistar Akibat Pemberian Natrium Nitrit Dyah Ayu Widyastuti; Maria Asih Ristianti; Ika Maya Sari
JURNAL ILMU KEFARMASIAN INDONESIA Vol 17 No 1 (2019): JIFI
Publisher : Fakultas Farmasi Universitas Pancasila

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (498.136 KB) | DOI: 10.35814/jifi.v17i1.560

Abstract

Sodium nitrite is one of common food addition in most meat product. This kind of food preservative is allowed by Permenkes No. 722/Menkes/Per/IX/88 only in minimum doses due to its carcinogenic effect. The most targeted organ is kidney which is sensitive to chemical matter as nefrotoxin. When the kidney is damaged, the erytropoietin secretion to form erytrocite is disturbed. Physiological damage in kidney can be identified by the concentration of blood creatinin and urea. The objective of this research is to study about blood creatinin and urea concentration of Wistar rats which is induced by sodium nitrite. Two groups of Wistar rats were induced by two doses of sodium nitrite (11.25 and 22.50 mg/kg body weight, respectively) a day with one group of control. Blood serum of those three groups were then be analyzed for creatinin and urea concentration each week for three months. The result showed that creatinin concertration is fluctuative during the day one to day ninety. The average of creatinin concentration for two treated groups is not significantly lower than control group. Generally, the urea concentration is increasing for day fourteen to seventy seven then decreasing in day eighty four to ninety. However, there are no significant differences among three groups.
Pengembangan Kreativitas Pengolahan Sampah Plastik melalui Pemberdayaan Wanita di Kalisegoro Semarang Dyah Ayu Widyastuti; Rivanna Citraning Rachmawati; Atip Nurwahyunani
Jurnal Surya Masyarakat Vol 2, No 2 (2020): Mei 2020
Publisher : Universitas Muhammadiyah Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26714/jsm.2.2.2020.105-109

Abstract

Unprocessing household waste is one of environmental problem which has to be overcome by Kalisegoro society. People only depend on garbage transport service to process household waste henceforward to final disposal site. Those habit leads to unprocessing household waste in Kalisegoro and more contaminate the environment. Household waste processing training is required to enhance the ability of Kalisegoro society, especially for housewife so that the household waste can be handled well. This program intend to enhance creativity of housewife in Kalisegoro to process plastic from household waste. Plastic waste can be processed become handycrafts and reduce rubbish buildup which leads to environmental pollution. The participant of this program is household who incorporated in Dorang Dawis Anggrek 17 business unit in Kalisegoro Gunungpati Semarang.
Pengembangan Kreativitas Pengolahan Sampah Plastik melalui Pemberdayaan Wanita di Kalisegoro Semarang Dyah Ayu Widyastuti; Rivanna Citraning Rachmawati; Atip Nurwahyunani
Jurnal Surya Masyarakat Vol 2, No 2 (2020): Mei 2020
Publisher : Universitas Muhammadiyah Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (379.586 KB) | DOI: 10.26714/jsm.2.2.2020.105-109

Abstract

Unprocessing household waste is one of environmental problem which has to be overcome by Kalisegoro society. People only depend on garbage transport service to process household waste henceforward to final disposal site. Those habit leads to unprocessing household waste in Kalisegoro and more contaminate the environment. Household waste processing training is required to enhance the ability of Kalisegoro society, especially for housewife so that the household waste can be handled well. This program intend to enhance creativity of housewife in Kalisegoro to process plastic from household waste. Plastic waste can be processed become handycrafts and reduce rubbish buildup which leads to environmental pollution. The participant of this program is household who incorporated in Dorang Dawis Anggrek 17 business unit in Kalisegoro Gunungpati Semarang.
Pemberdayaan Wanita Tani Kabupaten Kudus dalam Pembuatan Saus Cabai (Capsicum anuum) Dyah Ayu Widyastuti; Fafa Nurdyansyah
Jurnal Surya Masyarakat Vol 1, No 2 (2019): Mei 2019
Publisher : Universitas Muhammadiyah Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (527.76 KB) | DOI: 10.26714/jsm.1.2.2019.81-85

Abstract

Produksi cabai (Capsicum anuum) di Kabupaten Kudus yang cukup melimpah belum banyak dimanfaatkan oleh masyarakat. Buah cabai dapat diolah menjadi produk saus sehingga meningkatkan nilai ekonominya. Pengolahan cabai menjadi saus cabai dapat meningkatkan nilai jual sekaligus menghindari pembusukan berlebih ketika musim panen. Pengolahan cabai ini juga dapat menjadi alternatif pemberdayaan wanita tani di Kabupaten Kudus sehingga keterampilan dan produktivitasnya meningkat. Program ini bertujuan untuk memberdayakan wanita tani di Kabupaten Kudus dalam pembuatan saus cabai demi meningkatkan produktivitas dan kesejahteraan ekonomi masyarakat. Pelaksanaan program dibagi menjadi tiga tahapan yaitu penyuluhan dan sosialisasi, pelatihan, serta monitoring dan evaluasi. Peserta pelatihan adalah ibu-ibu wanita tani Kabupaten Kudus yang cenderung tidak produktif. Antusiasme peserta pelatihan terlihat dari keterlibatan dan partisipasi mereka dalam setiap tahapan program. Peningkatan keterampilan pembuatan saus cabai melalui pemberdayaan wanita tani Kabupaten Kudus ini diharapkan dapat meningkatkan produktivitas dan tingkat ekonomi masyarakat yang selama ini bergantung pada sektor pertanian.Kata kunci: pemberdayaan, saus cabai, wanita tani.AbstractThe high production of chilli (Capsicum anuum) in Kudus is not utilized yet. Chilli can be processed to other products such as chilli ketchup so its economical value is increasing. Chilli processing to ketchup is improve its price and also inhibit putrefaction when in harvest time. Its chilli processing can also empower peasant women in Kudus so it would increase their skill and productivity. The aim of this program is to empower peasant women in Kudus to process chilli ketchup. The program is devide into three steps: counseling and socialization, training, also monitoring and evaluation. The participants are peasant women in Kudus whose productivity is not so high.The participants antusiasm showed in their involvements and partisipations in each steps. Productivity of society are expected to improve by empower Kudus peasant women to produce chilli ketchup. So, the economy level is also been increased by its empowering program.
Identifikasi Genera Bakteri Coliform Pada Air Sungai Desa Datar Kabupaten Jepara Al Manunal Akhnah; Dyah Ayu Widyastuti; Rivanna Citraning Rachmawati
Quagga : Jurnal Pendidikan dan Biologi Vol 14, No 2 (2022): QUAGGA : Jurnal Pendidikan dan Biologi
Publisher : Program Studi Pendidikan Biologi, FKIP Universitas Kuningan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25134/quagga.v14i2.5061

Abstract

Kualitas air di Indonesia mengalami penurunan dari tahun ketahun. Salah satu perairan yang mengalami penurunan kualitas air adalah sungai Desa Datar, Kecamatan Mayong, Kabupaten Jepara. Penurunan kualitas air disertai dengan adanya bakteri coliform dalam air menjadi salah satu penyebab munculnya berbagai penyakit yang diakibatkan air terkontaminasi bakteri coliform. Tujuan dari penelitian ini yaitu untuk megidentifikasi genera bakteri Coliform di sungai Datar. Penelitian ini dilakukan di Laboratorium Biologi UPGRIS pada 16 Agustus – 27 Agustus 2021. Subyek dalam penelitian ini adalah air sungai yang terindikasi tercemar coliform di Desa Datar, Kecamatan Mayong, Kabupaten Jepara. Jenis penelitian ini adalah deskriptif dengan cara pengidentifikasi bakteri coliform yang ada di Sungai Desa Datar dengan teknik Purposive Sampling digunakan dalam menentukan lokasi penelitian. Pengambilan sampel dilakukan di Sungai Desa Datar. Data diambil berdasarkan observasi atau survei langsung ke lokasi tempat yang dijadikan sampel, serta pemeriksaan terhadap sampel secara langsung di laboratorium. Berdasarkan hasil penelitian dapat diketahui bahwa terdapat 3 genera bakteri colifrom diantaranya yaitu Escherichia coli yang memiliki karakteristik berbentuk bulat, tepian rata, elevansi cembung, berwarna hinau metalik, perwarnaan Gram negatif, berbentuk batang, sel warna preparat berwarna merah muda, uji indol positif, dan uji TSIA dasar dan lereng berwarna kuning. Kedua bakteri genera Shigella mempunyai karakteristik berbentuk bulat, tepian rata, elevansi cembung, berwarna ungu, perwarnaan Gram negatif, berbentuk batang, sel warna preparat berwarna merah muda, uji indol negatif, dan uji TSIA dasar berwarna kuning, lereng berwarna merah. Bakteri genera Klebsiella dengan karakteristik karakteristik berbentuk bulat, tepian rata, elevansi cembung, berwarna merah muda, perwarnaan Gram negatif, berbentuk batang, sel warna preparat berwarna merah muda, uji indol negatif, dan uji TSIA Dasar dan lereng berwarna kuning.
PENGARUH VARIASI MASSA RAGI SACCHAROMYCES CEREVISIAE DAN LAMA FERMENTASI TERHADAP DENSITAS DAN RENDEMEN BIOETANOL ALANG-ALANG (Imperata Cy-lindrica) Dyah Ayu Widyastuti; Ipah Budi Minarti Minarti; Nurusshofaniyatil Ula
JITEK (Jurnal Ilmiah Teknosains) Vol 8, No 1/Mei (2022): JiTek
Publisher : Universitas PGRI Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26877/jitek.v8i1/Mei.12572

Abstract

Ketersediaan energi fosil dalam penggunaan bahan bakar semakin menipis dan tingkat residu yang dihasilkan semakin menigkat, sehingga diperlukan sumber energi alternatif untuk menunjang kebutuhan energi salah satunya bioetanol. Alang- alang (Imperata cylindrica) memiliki kandungan selulosa sebanyak 40,22% dapat digunakan sebagai bahan dasar bioetanol dengan bantuan Saccharomyces cerevisiae pengubah glukosa menjadi etanol. Tujuan penelitian ini untuk menganalisis karakteristik bioetanol berdasarkan densitas dan persen rendemen bioetanol dengan variasi massa ragi roti Saccharomyces cerevisiae dan waktu fermentasi. Penelitian ini menggunakan Rancangan Acak Lengkap (RAL), dengan 4 perlakuan dan 3 kali ulangan. Jumlah takaran larutan untuk setiap perlakuan adalah 100 ml. Nilai densitas tertinggi pada perlakuan 4 massa   roti Saccharomyces cerevisiae 15 gram dengan lama fermentasi 6 hari sebesar 0.9041 g/ml, nilai densitas terendah pada perlakuan 1 massa ragi roti Saccharomyces cerevisiae 10 gram dan lama fermentasi 3 hari sebesar 0,9340 g/ml. Persen rendemen tertinggi diperoleh dari perlakuan 4 massa ragi roti Saccharomyces cerevisiae 15 gram dan lama fermentasi 6 hari sebanyak 23,11% dan persen rendemen terendah pada perlakuan 1 dengan massa ragi roti Saccharomyces cerevisiae 10 gram dan lama fermentasi 3 hari sebesar 17,90%. Hasil penelitian menunjukkan ada pengaruh variasi konsentrasi  Saccharomyces cerevisiae dan lama fermentasi terhadap nilai densitas dan persen rendemen.
Efektivitas Eceng Gondok (Eichhornia crassipes) dan Feses Sapi Sebagai Bahan Baku Produksi Biogas Firya Luthfiyah; Endah Rita Sulistyo Dewi; Dyah Ayu Widyastuti
Bioeksperimen: Jurnal Penelitian Biologi Vol 8, No 2: September 2022
Publisher : Universitas Muhammadiyah Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23917/bioeksperimen.v8i2.15118

Abstract

Masyarakat membutuhkan energi yang ramah lingkungan dan tidak mahal. Mengatasi adanya harga biogas yang mahal perlu adanya penelitian energi alternatif yaitu biogas dari limbah eceng gondok (Eichhornia crassipes) dan kotoran sapi. Penelitian ini bertujuan menganalisis volume dan rasio C/N  biogas yang paling optimal dari bahan baku eceng gondok (E. crassipes), kotoran sapi, dan campuran keduanya. Rancangan yang digunakan adalah Rancangan Acak Lengkap (RAL). Materi penelitian terdiri 3 perlakuan dan 3 pengulangan. Hasil penelitian menunjukkan bahwa ada pengaruh penggunaan variasi bahan baku antar perlakuan, dimana H0 menyatakan bahwa ada pengaruh penggunaan variasi bahan baku eceng gondok, kotoran sapi dan campuran keduanya terhadap volume dan rasio C/N biogas yang dihasilkan diterima. Jadi ada beda nyata antara volume biogas dan rasio C/N baik pada P1, P2 dan P3.
Fraksinasi Ekstrak Metanol Buah Parijoto (Medinilla speciosa Blume) dengan Pelarut Metanol, Etil Asetat, dan N-Heksana Nailil Mona; Dyah Ayu Widyastuti; Atip Nurwahyunani; Fafa Nurdyansyah
Florea : Jurnal Biologi dan Pembelajarannya Vol 9, No 2 (2022)
Publisher : UNIVERSITAS PGRI MADIUN

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25273/florea.v9i2.13883

Abstract

Parijoto fruit (Medinilla speciosa Blume) is an endemic fruit around Mount Muria, Kudus, Central Java. The native people utilize Parijoto as traditional herbal medicine. Those utilization due to its metabolite compounds, such as tannin, flavonoid, fenolic, terpenoid, etc. In order to utilize Parijoto fruit properly, it is necessary to have an appropriate extraction and fractination method. So, the bioactive compounds of Parijoto fruit can be optimally extracted. The objective of this study is to obtain fractions of Parijoto fruit using three different solvent, methanol, ethyl acetate, and n-hexane. This study perform maceration as extraction method and liquid-liquid fractination to obtain the fractions. The dry simplicia yield was 85.49%. The maceration result was in the form of a blackish red green liquid with 67.45% yield. The methanol fraction was a thick blackish red liquid with 45.4% yield. While, the ethyl acetate fraction was a slightly fibrous viscous liquid with pinkish milk brown colour and 22.8% yield. On the other hand, the n-hexane fraction was a greenish viscous liquid with 13.8% yield. The highest yield is the result of fractination with methanol as a solvent because it is the most polar among others in this study. The solvent polarity is greatly affects the yield of fractination from natural product materials. In this study, the order of Parijoto yield from the highest to the lowest is methanol fraction, ethyl acetate fraction, and the last is n-hexane fraction. Those results were obtained due to the differences of each solvent polarity.
Co-Authors Al Manunal Akhnah Atip Nurwahyunani, Atip Endah Rita Sulistyo Dewi Fafa Nurdyansyah Firya Luthfiyah Ika Maya Sari Ipah Budi Minarti Maria Asih Ristianti Nailil Mona Nurusshofaniyatil Ula Praptining Rahayu ., Praptining Rahayu Rivanna Citraning Rachmawati, Rivanna Citraning