Article Per Year (5 Year)
p-Index From 2020 - 2025
0
P-Index
This Author published in this journals
All Journal JURNAL PEMULIAAN TANAMAN HUTAN
Purnamila Sulistyawati
Balai Besar Penelitian dan Pengembangan Bioteknologi dan Pemuliaan Tanaman Hutan Yogyakarta
Agriculture, Biological Sciences & Forestry

Published : 4 Documents Claim Missing Document
Claim Missing Document
Check
Articles

Found 4 Documents
Search

 1 
STRUKTUR GENETIK POHON INDUK Calophyllum inophyllum DI TEGAKAN BENIH PROVENAN BERDASARKAN PENANDA SIMPLE SEQUENCES REPEATS ILG Nurtjahjaningsih; Purnamila Sulistyawati; Anto Rimbawanto
Jurnal Pemuliaan Tanaman Hutan Vol 13, No 1 (2019): Jurnal Pemuliaan Tanaman Hutan
Publisher : Center for Forest Biotechnology and Tree Improvement (CFBTI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (248.676 KB) | DOI: 10.20886/jpth.2019.13.1.45-51

Abstract

This study aimed to assess genetic structure of Calophyllum inophyllum using simple sequences repeats (SSR) markers to control genetic diversity at a provenance seed stand. Leaf samples were collected for DNA templates and 6 SSR primers were used. Genetic data of 280 trees were analyzed by FSTAT software, while PCoA was analyzed by GeneAlex. Results showed that number of allele (NA) per SSR primer between 3 and 4, the allelic richness (AR) ranged 2.805 to 4.000, the gene diversity (HE) ranged 0.018 to 0.631, coefficient inbreeding (FIS) of overall SSR primers were insignificant deviate from Hardy-Weinberg Equilibrium, excepted NY3. The mean HE values in the TBP was in low (mean HE= 0.272). However, the mean coefficient inbreeding values was not significant. PCoA analysis showed that the seed stand consisted genetically related trees; the 280 trees originated from only 5-6 mother trees. This is because the stand was originated from a conservation stand with narrow genetic differentiation. Management of the seed stand was discussed.
KEKERABATAN GENETIK ANAKAN ALAM ULIN (Eusideroxylon zwageri TEIJSM. & BINN.) MENGGUNAKAN PENANDA RANDOM AMPLIFIED POLYMORPHISM DNA ILG Nurtjahjaningsih; Sukartiningsih Sukartiningsih; Anna Puspa Amarta Saranti; Purnamila Sulistyawati; Anto Rimbawanto
Jurnal Pemuliaan Tanaman Hutan Vol 11, No 1 (2017): Jurnal Pemuliaan Tanaman Hutan
Publisher : Center for Forest Biotechnology and Tree Improvement (CFBTI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (411.592 KB) | DOI: 10.20886/jpth.2017.11.1.25-32

Abstract

The study aimed to assess genetic diversity and genetic relationship of ulin wildlings randomly collected from a nursery and originated from Bukit Soeharto natural forest, East Kalimantan. DNA templates were extracted from leaf samples of 1.5 years old wildings. Five RAPD primers consisted 55 polymorphic loci were used for genetic studies. Genetic diversity and relationship were analyzed using GenAlex software. The results showed moderate mean value of genetic diversity (HE=0,345, SE 0,015) of the wildings. Forty eight wildings were clustered in only 3 groups; almost all wildings (65%) were clustered in one main cluster. Moreover, 4 wildlings were clones (8%). In conclusion, the 48 wildings of ulin consisted high genetic relationship and individual clones that reflects the low genetic diversity of this species.
STRUKTUR GENETIK Calliandra calothyrsus DI INDONESIA MENGGUNAKAN PENANDA RANDOM AMPLIFIED POLYMORPHISM DNA (RAPD) ILG Nurtjahjaningsih; Purnamila Sulistyawati; Anto Rimbawanto
Jurnal Pemuliaan Tanaman Hutan Vol 10, No 1 (2016): Jurnal Pemuliaan Tanaman Hutan
Publisher : Center for Forest Biotechnology and Tree Improvement (CFBTI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20886/jpth.2016.10.1.31-38

Abstract

Calliandra calothyrsus was introduced to Indonesia from Guatemala in 1930s and had been planted widely throughout Indonesia for fuel wood. Genetic diversity within and among population is an important factor for initiating breeding program. Aim in this study was to evaluate genetic structure of C. calothyrsus in Indonesia, to obtain a better understanding of the history of the distribution and efficiency of breeding strategy of this species. Leaf samples were collected in a seedling seed orchard plot from 10 populations. Six RAPD markers consisted of 34 loci were used to analyze genetic diversity and genetic structure. The results showed that genetic diversity was in low to moderate level (mean HE= 0.321). The AMOVA analysis showed that genetic differentiation among geographical sources was significant, also among populations within geographical sources and among individual within populations as well. It was revealed that the 10 populations originated from only two ancestors. The limited number of ancestors resulted low to moderate genetic distances among populations (mean Da=0.070). In conclusion, C. calothyrsus has moderate genetic diversity within population and weak genetic structure among populations. Genetic diversity in population/individual level should be considered as a selection unit in the breeding strategies.
KERAGAMAN GENETIK POPULASI KAYU MERAH (Pterocarpus indicus Willd) MENGGUNAKAN PENANDA RAMDOM AMPLIFIED POLYMORPHISM DNA Purnamila Sulistyawati; AYPBC Widyatmoko
Jurnal Pemuliaan Tanaman Hutan Vol 11, No 1 (2017): Jurnal Pemuliaan Tanaman Hutan
Publisher : Center for Forest Biotechnology and Tree Improvement (CFBTI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (436.383 KB) | DOI: 10.20886/jpth.2017.11.1.67-76

Abstract

Kayu merah ( Pterocarpus indicus Willd) which listed as one of the IUCN Red List of Threatened Species in the category Vulnerable (VU A1D) is naturally distributed throughout Indonesia included Java, Sulawesi, Maluku, Bali, East Nusa Tenggara, West Nusa Tenggara and Papua. Random Amplified Polymorphic DNA (RAPD) molecular markers have been used to analyze the genetic diversity of six (6) natural populations of kayu merah from Seram Island, Manggarai Timur-Flores, Ngada-Flores, Soe-Timor Tengah Selatan, Kefamenanu-Timor Tengah Utara and Kupang. Ninety-nine (99) polymorphic loci were obtained from twelve RAPD primers. The mean of genetic diversity was 0.2024. Population with the highest genetic diversity was Soe (0.2925) and the lowest genetic diversity was Ngada (0.1212). The highest genetic distance among the populations was between Ngada and Kefamenanu (0.376), while the nearest genetic distance was between Ngada and Manggarai Timur (0.060). The informations resulted from this study are important to support the tree improvement and conservation programme of kayu merah.
Co-Authors Anna Puspa Amarta Saranti Anto Rimbawanto AYPBC Widyatmoko ILG Nurtjahjaningsih ILG Nurtjahjaningsih Sukartiningsih Sukartiningsih