R Firmansyah
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Journal : Jurnal Ilmu Ternak Veteriner

The residue of trenbolone from male Garut sheep which implanted by trenbolone acetate Widiastuti, R; ., Indraningsih; Murdiarti, T.B; Firmansyah, R
Indonesian Journal of Animal and Veterinary Sciences Vol 6, No 3 (2001)
Publisher : Indonesian Animal Sciences Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (139.817 KB) | DOI: 10.14334/jitv.v6i3.233

Abstract

Trenbolone acetate (TBA) is a growth hormone promoter which is implanted into animal to increase the body weight. The implantation of TBA in animal may cause the occurrence of residues of TBA and its metabolite (17-β-trenbolone). The presence of the residue might threat to human health. The aim of this research work was to study the presence of the residue of trenbolone in male Garut sheep which is implanted by TBA. The sheep were divided into 2 groups, those were D1 which were implanted by 40 mg TBA and D2 which were implanted by 60 mg TBA. One animal each from D1 and D2 were killed in the week 1, 2, 3 and 4 after implantation. And 24 hours before slaughtering the urine of D1 and D2 were collected using the metabolism cages. The samples for residue analysis were the organs such as liver, kidney, and the muscles around the implantation sites i.e inner and outer, upper front leg and upper back leg. The samples were extracted by organic solvents and analysed by HPLC. The residue of trenbolone occured in all samples of D1 and D2 which killed on 1 and 2 weeks after implantation. The residues occured on those which were killed 3 weeks after implantation were only in inner and outer samples. The residues in meat and organs were not detected in D1 nor D2 which were killed 4 weeks after implantation. The residue of trenbolone was also detected in the urine and still were detected in D1 and D2 which implanted by TBA for 4 weeks.   Key words : trenbolone acetate, 17-β-trenbolone, residue, sheep
Trenbolone residue in tissues and urine of Onggole male calves treated with acetate trenbolone implant Widiastuti, R; Firmansyah, R; ., Indraningsih
Indonesian Journal of Animal and Veterinary Sciences Vol 12, No 1 (2007)
Publisher : Indonesian Animal Sciences Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (195.159 KB) | DOI: 10.14334/jitv.v12i1.565

Abstract

Trenbolone acetate (TBA) is a hormone being permitted to be used as growth promoters for livestocks in several meat-exporting countries. The presence of trenbolone residu in animal products might affect human health. The purpose of this study was to determine the distribution of trenbolone residue (TBA dan 17 ß-trenbolone) in tissues and urine of Onggole male calves. The implantation of 200 mg TBA as Finaplix-H® was done subcutaneously on the back side of the medial part of ear. Urine were collected periodically until 21 days post implantation. The animals were terminated on the day 21st post implantation. Urine, meat and organs were analysed for trenbolone residues. The results showed that TBA residues were detected in tissues of inner and surrounding areas of the implantation sites and liver with an average concentration of 11 ng/g, 2,1 ng/g and 1,6 ng/g respectively. The 17 ß-trenbolone residue was only detected in tissue of inner area of the implantation site at the average concentration of 8,2 ng/g. Meanwhile, none of the residues were detected in urine. Key Words: Residue, Trenbolone, Implantation
The residue of trenbolone from male Garut sheep which implanted by trenbolone acetate R Widiastuti; Indraningsih .; T.B Murdiarti; R Firmansyah
Jurnal Ilmu Ternak dan Veteriner Vol 6, No 3 (2001): SEPTEMBER 2001
Publisher : Indonesian Center for Animal Research and Development (ICARD)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (139.817 KB) | DOI: 10.14334/jitv.v6i3.233

Abstract

Trenbolone acetate (TBA) is a growth hormone promoter which is implanted into animal to increase the body weight. The implantation of TBA in animal may cause the occurrence of residues of TBA and its metabolite (17-β-trenbolone). The presence of the residue might threat to human health. The aim of this research work was to study the presence of the residue of trenbolone in male Garut sheep which is implanted by TBA. The sheep were divided into 2 groups, those were D1 which were implanted by 40 mg TBA and D2 which were implanted by 60 mg TBA. One animal each from D1 and D2 were killed in the week 1, 2, 3 and 4 after implantation. And 24 hours before slaughtering the urine of D1 and D2 were collected using the metabolism cages. The samples for residue analysis were the organs such as liver, kidney, and the muscles around the implantation sites i.e inner and outer, upper front leg and upper back leg. The samples were extracted by organic solvents and analysed by HPLC. The residue of trenbolone occured in all samples of D1 and D2 which killed on 1 and 2 weeks after implantation. The residues occured on those which were killed 3 weeks after implantation were only in inner and outer samples. The residues in meat and organs were not detected in D1 nor D2 which were killed 4 weeks after implantation. The residue of trenbolone was also detected in the urine and still were detected in D1 and D2 which implanted by TBA for 4 weeks.   Key words : trenbolone acetate, 17-β-trenbolone, residue, sheep
Trenbolone residue in tissues and urine of Onggole male calves treated with acetate trenbolone implant R Widiastuti; R Firmansyah; Indraningsih .
Jurnal Ilmu Ternak dan Veteriner Vol 12, No 1 (2007): MARCH 2007
Publisher : Indonesian Center for Animal Research and Development (ICARD)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (195.159 KB) | DOI: 10.14334/jitv.v12i1.565

Abstract

Trenbolone acetate (TBA) is a hormone being permitted to be used as growth promoters for livestocks in several meat-exporting countries. The presence of trenbolone residu in animal products might affect human health. The purpose of this study was to determine the distribution of trenbolone residue (TBA dan 17 ß-trenbolone) in tissues and urine of Onggole male calves. The implantation of 200 mg TBA as Finaplix-H® was done subcutaneously on the back side of the medial part of ear. Urine were collected periodically until 21 days post implantation. The animals were terminated on the day 21st post implantation. Urine, meat and organs were analysed for trenbolone residues. The results showed that TBA residues were detected in tissues of inner and surrounding areas of the implantation sites and liver with an average concentration of 11 ng/g, 2,1 ng/g and 1,6 ng/g respectively. The 17 ß-trenbolone residue was only detected in tissue of inner area of the implantation site at the average concentration of 8,2 ng/g. Meanwhile, none of the residues were detected in urine. Key Words: Residue, Trenbolone, Implantation