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All Journal Jurnal Sain Veteriner Buletin Peternakan Jurnal Ilmu dan Teknologi Hasil Ternak Jurnal Pengabdian Kepada Masyarakat (Indonesian Journal of Community Engagement) TheJournalish: Social and Government Jurnal Triton Berkala Penelitian Teknologi Kulit, Sepatu, dan Produk Kulit
Nanung Agus Fitriyanto
Departemen Teknologi Hasil Ternak, Fakultas Peternakan Universitas Gadjah Mada, Jl. Fauna No.03, Karang Gayam, Caturtunggal, Depok, Sleman, D.I Yogyakarta 55281, Indonesia.
Agriculture, Biological Sciences & Forestry Arts Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Decision Sciences, Operations Research & Management Education Engineering Environmental Science Industrial & Manufacturing Engineering Law, Crime, Criminology & Criminal Justice Social Sciences Veterinary Other

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Determination of Cattle and Buffalo Skin Crackers Using Polymerase Chain Reaction Restriction Fragment Length Polymorphism Rulli Riana Dewi; Yuny Erwanto; Nanung Agus Fitriyanto
Jurnal Sain Veteriner Vol 35, No 2 (2017): Desember
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (6195.219 KB) | DOI: 10.22146/jsv.34667

Abstract

The aim of this study was to determine of cattle and buffalo species based on cytochrome b gene using PCR-RFLP. Cattle and buffalo hides were obtained from a slaughterhouse in Yogyakarta and Kudus Regency. To confirm the effectiveness and specificity of this fragment, there are seven of DNA mixture samples in various levels. Isolate DNA samples were amplified using universal primer of cytochrome b gene, then PCR amplicon was digested by RsaI restriction enzyme.. The result showed that mitochondrial cytochrome b gene successfully amplified fragments of 359 bp. RsaI restriction enzyme was able to cleave buffalo cytochrome b gene into two fragment  (326 and 23 bp), while the cytochrome b gene of the skin cattle DNA was uncleaved. . In conclusion, this study indicated that mixture DNA of cattle and buffalo hides could be digested by RsaI restriction enzyme  and determination of the buffalo hides in mixture samples could be detected into  10% level. Furthermore, RsaI enzyme could be used to specific identification buffalo species. PCR-RFLP technology has a potential and reliable method to identify  of the existence of r buffalo hides in the mixture with other hides.
Pengaruh Protease Aspergillus sp. pada Proses Soaking Kulit Domba Lokal terhadap Parameter Kualitas Fisik Kulit Semak Nanung Agus Fitriyanto; Suharjono Triatmojo; Yuny Erwanto
Buletin Peternakan Vol 28, No 3 (2004): Buletin Peternakan Vol. 28 (3) Agustus 2004
Publisher : Faculty of Animal Science, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21059/buletinpeternak.v28i3.1497

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Penerapan Protease Aspergillus sp pada Proses Buang Rambut Ramah Lingkungan Suharjono Triatmojo; Yuni Erwanto; Nanung Agus Fitriyanto
Buletin Peternakan Vol 28, No 4 (2004): Buletin Peternakan Vol. 28 (4) November 2004
Publisher : Faculty of Animal Science, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21059/buletinpeternak.v28i4.1507

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Biosorption of Metal Ions on Methanol Dehydrogenase Enzymatic Activity of Bradyrhizobium japonicum USDA110 Novita Kurniawati; Ambar Pertiwiningrum; Yuny Erwanto; Nanung Agus Fitriyanto; Mohammad Zainal Abidin
Buletin Peternakan Vol 42, No 2 (2018): BULETIN PETERNAKAN VOL. 42 (2) MAY 2018
Publisher : Faculty of Animal Science, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21059/buletinpeternak.v42i2.26195

Abstract

This research aims to understand the effect of metal ions bioabsorption which belong on different elemental groups to the methanol dehydrogenase (MDH) enzymatic activity in nitrogen-fixing bacteria Bradyhizobium japonicum USDA 110. Ten metal ions with each have 30μM concentration were added to grow Bradyhizobium japonicum USDA 110 in 10-1 diluted nutrient medium. The MDH activity test showed a similar result between the bacteria grown in medium without metal ions addition (control) and the bacteria were grown in a calcium ion/Ca2+ added media. The highest MDH enzymatic activity was shown on the bacteria grown in a magnesium/Mg2+ added medium, which showed 0.08 (U/mg) enzymatic activities. The addition of magnesium/Mg2+ metal ion accelerates the bacterial growth by 2.6 times and MDH activity by 1.28 times compared to control. The MDH enzyme is essential, especially for bacteria which exist in the soil environment, to adapt to high methanol concentration and to support bacterial anaerobic growth capacity along with plant symbiotic process. Moreover, the MDH activity staining method could also act as pollutant indicators like metal ions and hydrocarbon derivates. This research concluded that metal ions biosorption (calcium/Ca2+ and magnesium/Mg2+) are required for bacterial cells reproduction and oxidation of single carbon chain compounds like methanol. The nitrogen-fixing symbiotic bacteria, Bradyhizobium japonicum USDA 110 showed high MDH activity after the two metal ions absorption. However, contrary results were shown on vanadium/V3+, manganese/Mn2+, iron/Fe3+, copper/Cu2+, zinc/Zn2+, and aluminum/Al3+ absorption, which showed low MDH activity and cells biomass.
Direct Stimulation by Methanol Addition on the Cultured Medium for Methanol Dehydrogenase Protein Purification from Bradyrhizobium japonicum USDA110 Novita Kurniawati; Ambar Pertiwiningrum; Yuny Erwanto; Nanung Agus Fitriyanto; Mohammad Zainal Abidin
Buletin Peternakan Vol 42, No 3 (2018): BULETIN PETERNAKAN VOL. 42 (3) AUGUST 2018
Publisher : Faculty of Animal Science, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21059/buletinpeternak.v42i3.28155

Abstract

Methanol dehydrogenase (MDH) enzyme was purified from Bradyrhizobium japonicum USDA110 cell-free extract. The bacteria were grown in a culture medium with direct 0.5% methanol addition aimed to stimulates the MDH catalytic enzyme activation. Bradyrhizobium japonicum USDA110 MDH enzyme was purified by using 25 mM 2-(N-morpholine) ethanesulfonic acid/MES pH 5.5 buffer and 1 M sodium chloride/NaCl which separated into three columns, the first column was PD-10 for buffer exchange; the second column was HiTrap Sepharose HP to obtain unbonded fraction in the column; and the third column was Mono S 5/50 GL integrated with two pumps HPLC (high-performance liquid chromatography) to obtain pure MDH enzyme for serial changing of 1 M NaCl-25mM MES pH 5.5 with the flow rate at 1 ml/min. The protein concentration and MDH catalytic enzyme activity were observed on each purification process starting from the cell-free extract to pure MDH enzyme. The pure MDH enzyme was obtained by Mono S 5/50 GL-HPLC purification which showed a single band on SDS PAGE (sodium dodecyl sulfate-polyacrylamide gel electrophoresis). The MDH enzyme purification from Bradyrhizobium japonicum USDA110 showed 90-fold purification, a sub-molecular weight of 63 kDa, specific activity at 2.69 U/mg, and optimum activity at a 35oC temperature and pH 9.     
Penyuluhan dan Pendampingan Pengolahan Limbah Peternakan Sapi Potong di Kelompok Tani Ternak Sido Mulyo Dusun Pulosari, Desa Jumoyo, Kecamatan Salam, Kabupaten Magelang Nanung Agus Fitriyanto; Suharjono Triatmojo; Ambar Pertiwiningrum; Yuny Erwanto; Mohammad Zainal Abidin; Endang Baliarti; Yustina Yuni Suranindyah
Jurnal Pengabdian kepada Masyarakat (Indonesian Journal of Community Engagement) Vol 1, No 1 (2015): September
Publisher : Direktorat Pengabdian kepada Masyarakat Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (527.582 KB) | DOI: 10.22146/jpkm.16955

Abstract

Society services activity on cattle waste management system have been implemented in Sido Mulyo Livestock Farmers Group at Pulosari, Jumoyo, Salam, Magelang. Animal byproducts that consist of feces and urine of cattle wastewas processed into organic fertilizer compost and liquid fertilizer. Sido Mulyo Livestock Farmer Group has one unit of 20 m3 biodigester to accommodate the feces from approximately 30 cattle owned by the group member. Biogas has been used as a fuel source for family group members located around the cage. Slurry resulted from anaerobic digestion of biodigester disposed to pastures located on the right side of the cage. Ownership system in the groupis every group member hasa responsibility for taking care of their own cattle. The number of livestock owned by each member of the SidoMulyoLivestock Farmers Group ranged between 1 to 4 cattle. Society services methods that have been implemented was in the form of mentoring for a member of the Sido Mulyogroup.The other activities that have been implemented was the training and development of cattle industry, especially the handling of livestock waste in the form of feces, urine, and the feed residue. The activities was continued by the manufacture of compost packaging design, followed by the last series of activities such as monitoring and program development. The enthusiasm of the group members in joining to the extension activities is very good. The timing of the extension are determined in the afternoon after members of the group have finished searching feed for their cattle. The sustainability forwaste processing into organic fertilizer compost and liquid organic fertilizer becomes a major concern, because it is highly dependent on consumer demand.
IPTEK Bagi Masyarakat (IbM) Melalui Peningkatan Panen Pedet dan Produksi Kompos Berkualitas Pada Kelompok Ternak Sapi Potong Bayu Andri Atmoko; Endang Baliarti; Nanung Agus Fitriyanto
Jurnal Pengabdian kepada Masyarakat (Indonesian Journal of Community Engagement) Vol 5, No 1 (2019): April
Publisher : Direktorat Pengabdian kepada Masyarakat Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (445.014 KB) | DOI: 10.22146/jpkm.33874

Abstract

Beef Cattle have a role as saving for farmers to continue their business, although not profitable based on business analysis. Therefore, the right strategy is needed according to the problems of both the culture and the resources of farmers. The community service activity was conducted in a beef cattle farmer groups as cattle breeding for calf production, namely, are Mergo Andini Makmur and Sumber Ayu in Sleman Regency. The beef cattle productivity in the two groups is not good, S/C is more than twice, even up to four times, postpartum estrus is more than one year, and calving interval is more than 2 years. The beef cattle farmer groups have already diversified their business, which is the processing of manure and waste product of cattle, but not yet optimal. Community service is carried out to improve reproductive performance of cattle through the providing of a superior bull for bull stimulation, counseling, assistance in the prevention of worm infections, and demonstrations of compost and feed quality production. The first stage is preparation, including consolidation with the member farmer groups, followed by the socialization of the activity program. The next stage of implementation, by conducting reproductive and breeding management counseling, cattle health management, feed processing and management, and compost production. The result of the implementation of a bull stimulation for one month is based on the emergence of estrus (more than 50%) following by a conception rate (33.33%) of the cows population, and the level of participatory farmers. The demonstration of the fermented straw feed and compost is also implemented as a supporting activity.
Characterization of Kacang Goat skin Pepsin Soluble Collagen (Psc) and Their Potency as an Antioxidant Rina Wahyuningsih; Rusman Rusman; Nurliyani Nurliyani; Abdul Rohman; Nanung Agus Fitriyanto; Yuny Erwanto
Jurnal Ilmu dan Teknologi Hasil Ternak (JITEK) Vol. 16 No. 2 (2021)
Publisher : Faculty of Animal Science Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jitek.2021.016.02.1

Abstract

Collagen have been interesting material for many utilization such as food, pharmaceutical and cosmetic in various products and target administration, consequently collagen should be prepared as well as type of application. The objective of this research is to prepare collagen from goat skin and investigate the character and their potency as an antioxidant. Kacang goat skin aged 2 years was used for collagen production. Small slice skin was extracted by curing with 0.1% (w/v) pepsin in acetic acid 0.5 M, for 24, 48, dan 72 h at 4°C. The variables observed were molecular weight by Sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS PAGE), microstructure using scanning electron microscope, thermal stability by differential scanning calorimetric, and the antioxidant potency through 2,2-diphenyl-1-picrylhydrazyl (DPPH) inhibition analysis. The result showed the molecular weight range from 25 kDa to 180 kDa, microstructure showed the collagen fibril crosslink, collagen start to denature at 62,28°C, highest dissolved with 1% NaCl concentration and has highest DPPH inhibition at 60 min after hydrolysis. In conclusion, kacang goat skin collagen prepared by pepsin in acetic acid.
Perbandingan Metode Isolasi pada Deteksi Kulit Sapi, Kerbau, Kambing, dan Babi sebagai Bahan Baku Rambak Kulit Dyah Triasih; Rulli Riana Dewi; Yuny Erwanto; Nanung Agus Fitrianto
JURNAL TRITON Vol 11 No 1 (2020): JURNAL TRITON
Publisher : Politeknik Pembangunan Pertanian Manokwari

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.47687/jt.v11i1.100

Abstract

Kulit adalah hasil samping dari pemotongan ternak yang seiring waktu semakin meningkat permintaan konsumen bersamaan dengan meningkatnya pertumbuhan penduduk. Hal ini yang mendorong produsen berinovasi menciptakan produk baru. Salah satu inovasi produk yaitu pada rambak kulit. Tujuan dari penelitian ini untuk mengidentifikasi bahan dasar yang digunakan pada rambak kulit. Salah satunya dengan analisis genetik melalui penanda molekuler. Keberhasilan teknik molekuler ditentukan oleh tertangkapnya DNA genom dari sampel. Tahapan utama analisis genetik adalah isolasi DNA. Metode Sambrook dan metode Wasko yang telah dimodifikasi digunakan dalam penelitian ini. Sampel yang digunakan berupa kulit sapi, kerbau, kambing, dan babi dalam keadaan segar yang telah diawetkan dalam freezer dengan suhu -20oC. konsentrasi dan kemurnian DNA diukur dengan spektrofotometer pada λ260 dan λ280. Amplifikasi gen cytochrome b menggunakan primer universal. Elektroforesis menggunakan agarose sebesar 0,8% untuk isoalsi DNA dan 1,5% untuk amplifikasi PCR selama 30 menit dengan tegangan 100 volt. Berdasarkan hasil penelitian menunjukkan bahwa metode Sambrook yang telah dimodifikasi memberikan hasil yang baik, pita DNA yang dihasilnya terlihat secara jelas dibandingkan dengan menggunakan metode Wasko yang telah dimodifikasi. Konsentrasi dan kemurnian DNA metode Sambrook lebih baik dibandingkan dengan metode Wasko. Amplifikasi PCR gen cytochrome b menghasilkan produk PCR sebesar 359 bp pada kulit sapi, kerbau, kambing, dan babi.
Effect of Compost Tea and Harvest Age on Productivity, Nutrient Content, and In vitro Digestibility Cichorium Intybus Rezki Amalyadi; Nafiatul Umami; Nanung Agus Fitrianto; Chusnul Hanim; Bambang Suwignyo
Buletin Peternakan Vol 46, No 3 (2022): BULETIN PETERNAKAN VOL. 46 (3) AUGUST 2022
Publisher : Faculty of Animal Science, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21059/buletinpeternak.v46i3.67834

Abstract

The glass house experiment involving compost tea and harvest age was conducted to investigate the effect of giving compost tea (cricket and rabbit feces) and harvesting age on productivity, nutrient content, and in vitro digestibility value of Cichorium intybus. Compost tea (CT) is an aqueous extract from compost that can correct nutrient deficiencies during crop production and protect cultivation. The research treatment consisted of two factors, namely: the age of harvest and the dose of compost tea. The administration of compost tea consisted of three kinds of doses as follows: no compost tea (P0), 200 mL of compost tea (P1), and 400 mL of compost tea (P2). The experimental plants were harvested at different age, consisting of 25, 35 and 45 days after planting (DAP). The experimental design was Factorial Completely Randomized Design (CRD) with 6 treatments and 9 replications. The data were statictically analyzed using analisys of variance, and differences among value of each treatment were tested with the Duncan Multiple Range Test. The results indicated that compost tea dose and harvesting period treatment affected (P<0.05) leaf length, fresh leaf weight, crude protein content, dry matter digestibility (DMD), and organic matter digestibility (OMD). Meanwhile, it showed not significant effect (P>0.05) on the leaf width and root length. The results suggest that the fertilization treatment of 200 mL of compost tea and 25 DAP could increase productivity (leaf length and fresh leaf weight) of chicory, nutrient content (crude protein) of chicory, and in vitro digestibility (DMD and OMD).
Co-Authors Abdul Rohman Ambar Pertiwiningrum Amin Susiatmojo Bambang Suwignyo Bayu Andri Atmoko Chusnul Hanim Djarot Heru Santosa Dyah Triasih Endang Baliarti Mohammad Zainal Abidin Nafiatul Umami Novita Kurniawati Nurliyani Nurliyani R. L. M. Satrio Ari Wibowo Rezki Amalyadi Rina Wahyuningsih Rulli Riana Dewi Rulli Riana Dewi Rusman Rusman Rustamaji Rustamaji Sari Handayani Suharjono Triatmojo Suharjono Triatmojo Takashi Hayakawa Tomoyuki Nakagawa Yuni Erwanto Yuny Erwanto Yustina Yuni Surandiyah Zaenal Bachruddin