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All Journal Buletin Peternakan Microbiology Indonesia Jurnal Ilmu dan Teknologi Hasil Ternak Jurnal Sain Peternakan Indonesia Jurnal Ilmu-Ilmu Peternakan (Indonesian Journal of Animal Science) Jurnal Ilmu Ternak Veteriner Indonesian Journal of Biotechnology Jurnal Aplikasi Teknologi Pangan Jurnal Aplikasi Teknologi Pangan
Chusnul Hanim
Fakultas Peternakan, Universitas Gadjah Mada
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Education Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology Veterinary Other

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Partial Purifi cation, Stability Analysis, and Preservation of Xylanase from Xylanolytic Alkalophylic Bacteria Hanim, Chusnul; Cahyanto, Muhamad Nur; Yusiati, Lies Mira; Wibowo, Ali
Indonesian Journal of Biotechnology Vol 18, No 1 (2013)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (190.753 KB)

Abstract

A xylanase, which produces xylose from oat spelt xylans, was isolated from the culture medium of  xylanolytic alkalophylic bacteria mutant. The enzyme was purifi ed by ammonium sulphate with level 30, 40, 50, 60, 70, 80, and 90%. The purify of the fi nal preparation was demonstrated by sodium dodecyl sulphatepolyacrylamide gel electrophoresis. The molecular masses of the purifi ed xylanase were 137.61 and 165.34 kDa. Result of ammonium sulphate saturation with the highest activity was used as standart for saturation for enzyme production and preservation, using corn, tapioca, soy bean meal and gaplek fl our as carriers. Addition of 60% ammonium sulphate showed the highest xylanase activity (62.03 U/g), and produced 89.40% enzyme recovery. Tapioca, as a carrier, produced the highest xylanase activity.Key words: preservation, purifi cation, stability analysis, xylanase.
Effects of Probiotic, Prebiotic, and Synbiotic Mixed Culture Based on Wheat Pollard on Productivity of Kampung’s Chicken Cahya Setya Utama; Zuprizal Zuprizal; Chusnul Hanim; Wihandoyo Wihandoyo
Jurnal Ilmu Ternak dan Veteriner Vol 25, No 4 (2020): December 2020
Publisher : Indonesian Center for Animal Research and Development (ICARD)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14334/jitv.v25i4.2499

Abstract

This research was aimed to assess the effectiveness of processed wheat pollard -based rations to increase the productivity of Kampung chickens raised until 8 weeks old. The research was carried out in a completely randomized design with 5 treatments and 4 replications. The treatments consisted of wheat pollard based ration (WP), wheat pollard based ration plus probiotic mixed culture (WPPro), wheat Pollard as prebiotic mixed (WPPre)            , wheat pollard as synbiotic mixed culture ration 40% (WPS40), wheat pollard as synbiotic mixed culture ration 60% (WPS60)   . The parameters observed were feed consumption, final body weight, feed conversion ratio (FCR), weight gain, nitrogen retention, income over feed and chick cost (IOFCC) and profiles of intestinal villus. Results showed a significant effect of the treatments on the final body weight, weight gain, nitrogen retention, IOFCC and profiles of small intestinal villus (duodenum, jejenum, ileum). It was concluded that the inclusion of 40% wheat pollard synbiotic mixed culture (WPS 40) in the ration was able to increase the productivity of kampung chickens reared until 8 weeks old.
Partial Purifi cation, Stability Analysis, and Preservation of Xylanase from Xylanolytic Alkalophylic Bacteria Chusnul Hanim; Muhamad Nur Cahyanto; Lies Mira Yusiati; Ali Wibowo
Indonesian Journal of Biotechnology Vol 18, No 1 (2013)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (190.753 KB) | DOI: 10.22146/ijbiotech.7861

Abstract

A xylanase, which produces xylose from oat spelt xylans, was isolated from the culture medium of  xylanolytic alkalophylic bacteria mutant. The enzyme was purifi ed by ammonium sulphate with level 30, 40, 50, 60, 70, 80, and 90%. The purify of the fi nal preparation was demonstrated by sodium dodecyl sulphatepolyacrylamide gel electrophoresis. The molecular masses of the purifi ed xylanase were 137.61 and 165.34 kDa. Result of ammonium sulphate saturation with the highest activity was used as standart for saturation for enzyme production and preservation, using corn, tapioca, soy bean meal and gaplek fl our as carriers. Addition of 60% ammonium sulphate showed the highest xylanase activity (62.03 U/g), and produced 89.40% enzyme recovery. Tapioca, as a carrier, produced the highest xylanase activity. Key words: preservation, purifi cation, stability analysis, xylanase.
Optimalisasi Pertumbuhan Bakteri Xilanolitik dari Ketam (Eriocheir sinensis) Chusnul Hanim
Buletin Peternakan Vol 27, No 4 (2003): Buletin Peternakan Vol. 27 (4) November 2003
Publisher : Faculty of Animal Science, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21059/buletinpeternak.v27i4.1482

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Evaluasi Nilai Nutrisi Bungkil Inti Kelapa Sawit yang Difermentasi dengan Jamur Chusnul Hanim; Zaenal Bachrudin; Ali Agus
Buletin Peternakan Vol 23, No 2 (1999): Buletin Peternakan Vol. 23 (2) Mei 1999
Publisher : Faculty of Animal Science, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21059/buletinpeternak.v23i2.1650

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PENGGUNAAN PROTEASE DALAM PAKAN YANG MENGGUNAKAN LIMBAH PERTANIAN-PETERNAKAN UNTUK MENINGKATKAN KINERJA PERTUMBUHAN AYAM BROILER Apriliana Devi Anggraini; Ferry Poernama; Chusnul Hanim; Nanung Danar Dono
Buletin Peternakan Vol 41, No 3 (2017): BULETIN PETERNAKAN VOL. 41 (3) AGUSTUS 2017
Publisher : Faculty of Animal Science, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21059/buletinpeternak.v41i3.10755

Abstract

This study was aimed to determine the effects of protease supplementation in diets with agricultural-livestock by products on the growth performance in broiler chickens. The commercial protease used in current study was produced by Bacillus licheniformis which based on keratinase. The treatment diets were a control basal diet without agricultural-livestock by-products and protease supplementation (P1); P1 + 0.05% protease; basal diet with meat bone meal (MBM) and distiller’s dried grain with soluble (DDGS) suppementation (P3); P3 + 0.05% protease (P4); basal diet with hydrolized chicken feather meal (HCFM) and DDGS supplementation (P5); P5 + 0.05% protease. The obtained data were statistically analyzed using Complete Randomized Design, and analyzed subsequently by Orthogonal Contrats Test. Results showed that protease supplementation in the diets containing agricultural-livestock by-products did not affect growth performance of broiler chickens in starter phase and overall age phase. However, in diets containing MBM, protease supplementation reduced the nett gain and feed conversion ratio (FCR) in finisher phase (P<0.05). In diets containing HCFM, protease supplementation increased nett gain and FCR (P<0.05), compared to those of containing MBM. It could be concluded that qualified HCFM, MBM, and DDGS can be used as alternatives for protein-energy source feed stuffs in starter phase, as they did not reduce growth performance of broiler chickens. Protease used in this study might be more effective in diets containing HCFM than those of containing MBM and DDGS.Keywords: Agricultural-livestock by-products, Broilers chickens, Growth performance, Protease.
Effect of Cashew Nutshell Oil Supplementation as Phenol Source for Protein Protection on In Vitro Nutrient Digestibility Rahma Fitriastuti; Lies Mira Yusiati; Budi Prasetyo Widyobroto; Zaenal Bachruddin; Chusnul Hanim
Buletin Peternakan Vol 43, No 4 (2019): BULETIN PETERNAKAN VOL. 43 (4) NOVEMBER 2019
Publisher : Faculty of Animal Science, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21059/buletinpeternak.v43i4.35591

Abstract

The current study was aimed to evaluate the ability of phenol content in cashew nutshell liquid (CNSL) in binding protein and its effect on in vitro nutrient digestibility. This research divided into three steps, there were determination of total phenol and tannins content in CNSL, optimalization binding of CNSL tannin to bovine serum albumin with the determination of protein-percipitable phenolics compound and protein content using Lowry method, after that amount of optimalization supplementation of CNSL was used for measuring on in vitro nutrient digestibility by Tilley and Terry method. The results showed that phenol and tannin content of CNSL were 148.69 g/100 ml and 28.3 g/100 ml. One gram tannin in CNSL could be bound by 65.83 g protein. Optimum tannin were used in this study was 5.85 g tannin or equivalent to 163.91 mg of CNSL. Amount of CNSL were used in various levels, there are 0, 50, 100, 150 dan 200 mg. CNSL supplementation reduced dry matter, organic matter crude protein digestibility in rumen, and didn’t reduce dry matter dan crude protein in vitro post rumen digestibility. Supplementation of CNSL increased feed efficiency on in vitro.
Comparison of Nutrient Digestibility of Bligon and Kejobong Goats Fed by King Grass and Peanut Straw Chusnul Hanim; Lies Mira Yusiati; I Gede Suparta Budisatria; Fandi Widya Rachman
Buletin Peternakan Vol 44, No 1 (2020): BULETIN PETERNAKAN VOL. 44 (1) FEBRUARY 2020
Publisher : Faculty of Animal Science, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21059/buletinpeternak.v44i1.36229

Abstract

This experiment aimed to determine the nutrient digestibility of Bligon and Kejobong goats fed by king grass and peanut straw. The sample used was 6 to 8 months of five male Bligon goats, and five male Kejobong goats with the initial body weight of 15 to 20 kg and placed in separated metabolism cages. The diet and water were supplied ad libitum. This experiment was done in 14 days of adaptation period and seven days of the collection period. In the collection period, samples of feed, refusal feed, and feces were collected to get the chemical content includes dry matter (DM), organic matter (OM), ether extract (EE), crude fiber (CF), crude protein (CP), nitrogen-free extract (NFE), and total digestible nutrient (TDN). The data obtained were subjected to a T-test analysis. Results showed that nutrients intake, digested nutrient as well as nutrient digestibility of Bligon and Kejobong goats were not significantly different. However, Kejobong goats had EE and CP intake of 15.42% and 14.02%, digested DM, OM, and NFE of 16.29%, 15.71%, and 13.93% respectively, as well as NFE digestibility 4.37% higher than those Bligon goats (P< 0.05). Therefore, there was no difference in nutrient intake, digested, and digestibility of nutrients in Bligon and Kejobong goats fed by king grass and peanut straw. However, Kejobong goats appeared to have higher EE and CP intake, as well as digested and digestibility of DM, OM, and NFE than Bligon goats. 
Bacteriocin Activity of Lactic Acid Bacteria Isolated from Rumen Fluid of Thin Tail Sheep Okti Widayati; Zaenal Bachruddin; Chusnul Hanim; Lies Mira Yusiati; Nafiatul Umami
Buletin Peternakan Vol 43, No 3 (2019): BULETIN PETERNAKAN VOL. 43 (3) AUGUST 2019
Publisher : Faculty of Animal Science, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21059/buletinpeternak.v43i3.36837

Abstract

The objective of this study was to determine the activity and the stability of bacteriocin from lactic acid bacteria (BAL) isolated from rumen fluid of thin-tail sheep under the temperature (80, 100, and 121°C), pH (3, 7, and 10), and the length of storage (for 2 weeks under the temperature -8, 11, and 29°C). Lactic acid bacteria obtained by isolation, selection, and identification of thin-tailed sheep rumen fluid were used for bacteriocin production. The crude bacteriocin was partially purified using 70% ammonium sulfate, then was dialysis for 12 hours. The obtained bacteriocin then tested its inhibitory activity against E.coli (representing Gram-negative) and S. aureus (representing Gram-positive) under temperature (80, 100, and 121°C), pH (3, 7, and 10), and the length of storage (for 2 weeks under the temperature -8, 11, and 29°C). The data of bacteriocin activity based on pH, temperature, and the length of storage were analyzed with factorial, then when there was a significant difference of variable because treatment was continued with Duncan's Multiple Range Test (DMRT) test. The results showed that the bacteriocin activity of the three types of BAL against S.aureus is greater than E.coli. The highest activity was shown in pH 3, while the lowest activity was shown at pH 10 (P<0.01). The highest activity was shown at a heating temperature of 100°C, while the lowest activity was shown at a heating temperature of 80°C (P<0.01). The activity of bacteriocin produced by BAL 0 A, BAL 1 A, and BAL 4 C tended to be stable to the heating temperature of 80, 100, and 121°C but decreased with increasing pH value (pH 3, 7, and 10). The best of bacteriocin activity was found at pH 3 (acid), heating at 100°C, and stored at -8°C for 14 days.
The Effect of Nutmeg Leaves Tannin (Myristica fragrans Houtt) as Protein Protecting Agents on In Vitro Nutrient Digestibility Monica Canadianti; Lies Mira Yusiati; Chusnul Hanim; Budi Prasetyo Widyobroto; Andriyani Astuti
Buletin Peternakan Vol 44, No 1 (2020): BULETIN PETERNAKAN VOL. 44 (1) FEBRUARY 2020
Publisher : Faculty of Animal Science, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21059/buletinpeternak.v44i1.47976

Abstract

This experiment was aimed to study the effect of nutmeg leaf tannin addition on in vitro nutrient digestibility. Treatments in this experiment consisted of: P0 (control without tannin), P1 (feed + 2% tannin) and P2 (feed + 4% tannin). Feed for fermentation substrate consisted of Pennisetum purpureum and soybean meal with ratio 60:40. Fermentation was carried out using Tilley and Terry two stages in vitro technique for 48 hours. Variables measured were the digestibility of dry matter, organic matter, and crude protein in the rumen as well as the total digestive tract digestibility based on in vitro technique. The data obtained were analyzed by One Way ANOVA, and followed by the Duncan Multiple Range Test (DMRT). The results showed that rumen dry matter digestibility was lower (P<0.05) in P1 and P2 (59.03±3.24 and 57.19±1.32) compared to P0 (70.77±1.05), but did not show a significant difference (P>0.05) in the total dry matter digestibility of P0, P1, and P2 (74.88±5.28, 67.70±3.21, and 64.83±4.96). Organic matter digestibility in the rumen was also lower (P<0.05) in P1 and P2 (55.55±6.29 and 55.76±6.88) compared to P0 (75.39±0.91), but did not show significant difference (P>0.05) in total organic matter digestibility from P0, P1, and P2 (64.69±6.44, 64.33±6.34, and 61.20±5.11). The digestibility of crude protein in the rumen at P1 and P2 (45.48±5.12 and 38.47±3.44) was also significantly lower (P<0.05) compared to P0 (60.93±9.72), whereas total digestibility did not show any significant difference (P>0.05). Addition of tannin leaf nutmeg 2% optimally reduced rumen dry matter and crude protein digestibility without causing excessive negative impact on results of in vitro digestibility, so it can be used as a protective agent protein feed.
Co-Authors Adhe Humaera Ali Agus Ali Agus Ali Wibowo Ali Wibowo Ali Wibowo Andriyani Astuti Apriliana Devi Anggraini Asih Kurniawati Asri Nurul Huda Aulia Puspita Anugra Yekti Bambang Ariyadi Bambang Suwignyo Baskara, Aji Praba Budi Prasetyo Widyobroto Budi Prasetyo Widyobroto Cahya Setya Utama Cahya Setya Utama Canadianti, Monica Edi Suryanto Erika Kusumawardani Fandi Widya Rachman Ferry Poernama Gardika Windar Prahara I Gede Suparta Budisatria Ika Sumantri Jamhari Jamhari Joko Riyanto Jumari Jumari Lies Mira Yusiati Mashudi Mashudi Muhamad Nur Cahyanto Muhamad Nur Cahyanto, Muhamad Nur Muhammad Fathin Hanif Muhammad Nur Cahyanto Muhammad Zainul Hanif Muhsin Al Anas Nafiatul Umami Nanung Agus Fitriyanto Nanung Danar Dono Nur Zaini Putri Utami Rahma Fitriastuti Rezki Amalyadi Rusman Rusman Rusman Siti &#039;Athiya Wibowo Siti Zubaidah Sylvia C. H. Hehanussa Theresia Nur Indah Koni Trinil Susilawati Widayati, Okti Wihandoyo Wihandoyo Wihandoyo, Wihandoyo Winny Swastike Yuny Erwanto Zaenal Bachruddin Zaenal Bachrudin Zaenal Bachrudin Zuprizal (Zuprizal)