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All Journal Techno: Jurnal Penelitian BERITA BIOLOGI Jurnal Matematika Sains dan Teknologi Jurnal Farmamedika (Pharmamedica Journal) Journal of Tropical Ethnobiology Indonesian Community Journal SAINSMAT: Jurnal Ilmiah Ilmu Pengetahuan Alam Jurnal Kedokteran Hewan
Budi Prasetyo
Program Studi Biologi Fakultas Matematika Dan Ilmu Pengetahuan Alam Universitas Terbuka, Jakarta
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemistry Civil Engineering, Building, Construction & Architecture Computer Science & IT Decision Sciences, Operations Research & Management Economics, Econometrics & Finance Education Environmental Science Immunology & microbiology Mathematics Medicine & Pharmacology Physics Public Health Social Sciences Veterinary Other

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IDENTIFIKASI GEN ENTEROTOKSIN DAN EXFOLIATIF ISOLAT Staphylococcus aureus ASAL SUSU SAPI PERAH DAN SUSU KAMBING DARI BOGOR Budi Prasetyo
Jurnal Matematika Sains dan Teknologi Vol. 16 No. 2 (2015)
Publisher : LPPM Universitas Terbuka

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (361.256 KB)

Abstract

In humans, Staphylococcus aureus is an important pathogen as the cause of many cases of diseases such as food poisoning, skin infections, endocarditis, pneumonia, osteomyelitis, septic arthritis, and enshefalitis. The experiment was conducted at the Laboratory Biotrop SEAMEO, Bogor aims to detect enterotoxin and exfoliative genes from Staphylococcus aureus isolates origin dairy cow milk and goat milk from the village of Cijeruk, Bogor. Research methods include preparation of DNA, 23S rRNA gene amplification, enterotoxin gene amplification and exfoliative gene amplification. Results of enterotoxin gene amplification (sea, seb) and exfoliative gene amplification (eta) against 2 isolates of S. aureus showed positive results, but negative for the exfoliative gene (etb). A positive result was indicated by the appearance of DNA fragments that have a specific length (121 bp sea, seb 477 bp, and 119 bp eta) according to the PCR products of reference and GeneBank database. It is concluded that the gene has been detected enterotoxin (sea, seb) and exfoliative (eta) on isolates of S. aureus origin dairy cow milk and goat milk from the village of Cijeruk, Bogor. Pada manusia, Staphylococcus aureus merupakan patogen penting sebagai penyebab timbulnya berbagai kasus penyakit seperti keracunan makanan, infeksi kulit, endokarditis, pneumonia, osteomielitis, sepsis artritis, dan enshefalitis. Penelitian dilaksanakan di Laboratorium Seameo Biotrop, Bogor bertujuan mendeteksi gen enterotoksin dan exfoliatif isolat Staphylococcus aureus asal susu sapi perah dan susu kambing dari Desa Cijeruk, Bogor. Metode penelitian meliputi preparasi DNA, amplifikasi gen 23S rRNA, amplifikasi gen enterotoksin dan exfoliatif. Hasil amplifikasi gen enterotoksin (sea, seb) dan exfoliatif (eta) terhadap 2 isolat S. aureus menunjukkan hasil positif, tetapi negatif terhadap gen exfoliatif (etb). Hasil positif tersebut ditandai dengan munculnya fragmen DNA yang memiliki panjang spesifik (sea 121 bp, seb 477 bp, dan eta 119 bp) sesuai dengan produk PCR dari referensi dan database GeneBank. Disimpulkan bahwa telah dideteksi gen enterotoksin (sea, seb) dan exfoliatif (eta) pada isolat S. aureus asal susu sapi perah dan susu kambing dari Desa Cijeruk, Bogor.
LINGKUNGAN FISIK DAN KEKAYAAN MIKROALGA DI DANAU UNIVERSITAS TERBUKA, TANGERANG SELATAN Budi Prasetyo
Jurnal Matematika Sains dan Teknologi Vol. 14 No. 2 (2013)
Publisher : LPPM Universitas Terbuka

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1272.613 KB) | DOI: 10.33830/jmst.v14i2.385.2013

Abstract

Lake of Open University (UT) is designed not only as a container, watershed, irrigation, sports, and recreation, but also as critical habitat for the survival of plants and aquatic animals ranging from algae groups to types of vertebrate. Information relating to the data microalgae and environmental conditions of lake UT physical and chemical have not been investigated. Research objectives were to measure the wealth of microalgae that live in Lake UT, the microalgae diversity, and the physical and chemical environment. The method used to identify each kind of microalgae, is by counting the number of individuals microalgae, and analyze the water quality. Measurement results showed that the brightness of the physical environment of the lake water had turbid category (52-60 cm). The temperature of the lake water showed 31 to 31.5 oC, this temperature range is good for the growth of microalgae. Value of the degree of acidity of water was 7.3 to 7.5. DO measurements generating valued from 0.5 to 0.57 mg/L. The wealth of microalgae that live in lake UT was identificated as many as 21 genera at four stations with a total population of 4.080 individual/mm3. The highest percentage individual was Scenedesmus sp. Species richness index was 3,33 (medium category). Highest individual density was found in th middle of the lake by 4.651 individualLitre, followed by a passage from edge of the lake to the edged of gazibu (2.318 individual/Litre), the edge of lake (2.025 individual/Litre), and the last outlet (1.193 individual/Litre) lake. Biodiversity index is quite low at 2.107. Desain pembuatan danau UT di samping sebagai tempat resapan air, wahana rekreasi, sarana olahraga, juga merupakan habitat penting bagi tumbuhan dan hewan air (golongan alga sampai jenis vertebrata). Informasi mengenai data mikroalga serta kondisi fisik dan kimiawi lingkungan danau UT belum diketahui sehingga perlu dilakukan penelitian. Tujuan penelitian untuk mengukur kekayaan mikroalga, keanekaragaman mikroalga, dan kondisi lingkungan fisik dan kimiawi. Metode yang digunakan adalah mengidentifikasi setiap jenis dan menghitung jumlah individu mikroalga, serta menganalisis kualitas air. Hasil pengukuran kecerahan air danau dikatakan relatif keruh (52-60 cm). Pengukuran suhu air menunjukkan besaran angka 31-31,5oC, kisaran suhu tersebut baik bagi pertumbuhan mikroalga. Nilai derajat keasaman air adalah 7,3-7,5, sedangkan hasil pengukuran DO sebesar 0,5-0,57 mg/L. Kekayaan mikroalga yang teridentifikasi di danau UT sebanyak 21 genus ditemukan di empat stasiun dengan total populasi 4.080 individu/mm3. Persentase jumlah individu terbanyak adalah Scenedesmus sp. Indeks kekayaan jenis termasuk kategori sedang (3,33). Kepadatan individu tertinggi ditemukan di stasiun-2 (bagian tengah) sebesar 4.651 individu/Liter, selanjutnya diikuti oleh stasiun-3 dari tepi danau sampai tepi gasebo (2. 318 individu/Liter), stasiun-1 di bagian tepi danau (2.025 individu/Liter), dan stasiun-4 di bagian keluaran air (1.193 individu/Liter). Indeks biodiversitas jenis cukup rendah yaitu 2,107.
PROFIL VEGETASI PEKARANGAN DI DESA JABON MEKAR, KECAMATAN PARUNG, BOGOR Budi Prasetyo
Jurnal Matematika Sains dan Teknologi Vol. 8 No. 1 (2007)
Publisher : LPPM Universitas Terbuka

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Abstract

Jabon Mekar village is well-known by people as the central of fruit producing at subdistrict of Parung. However it was estimated as a buffer zone of Jakarta and subject of the urban development. The aim of the research is to study the vegetation profile and the tree of model architecture at home-garden of community in the village of Jabon Mekar. The methods used for vegetation analysis were the quadrate method and the vegetation profile architecture method. The result of the vegetation profile at home-garden research shows that according to their formation of canopy coat, there were five stratums. Based on the vertical and horizontal of profile diagram, the dominant species of plants is Gnetum gnemon, with Fagerlind model of architecture; and the co-dominant species of plants is Musa sp., with Tomlinson model of architecture. The domination of these two trees of architecture models will give a limited chance of having an erotion process at the home-garden society.
PERSPEKTIF GENDER SUKU OSING DI BANYUWANGI DALAM PENILAIAN KEMANFAATAN TANAMAN Budi Prasetyo; Tatik Chikmawati; Eko Baroto Walujo; Ervizal A.M Zuhud
BERITA BIOLOGI Vol 18, No 2 (2019)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v18i2.3689

Abstract

The different roles and responsibilities of male and female of the Osing tribe influence the knowledge of the use of plants. The aims of research is to analyze the values of the benefits of plants based on gender perception. The research used a qualitative and quantitative methods. Data collection for the value of plant species utilization were carried out  using Pebble Distribution Method (PDM) and analysis used Index of Cultural Significance (ICS). The Osing community recognised 86 plant species that were grouped in to eight beneficial groups. The male in three villages have a better understanding of ethnobotanical knowledge and practice than female on crops as staple foods, medicines, fruits, and firewood, while understanding of ornamental plants are relatively the same for both male and female. Female have a broader and more detailed understanding on vegetables, plant species used as building construction, and traditional rituals. The high PDM value in eight beneficial groups was supported by the high value of each ICS.  
Traditional Wisdom and Conservation of the Osing Tribal Community in Banyuwangi Budi Prasetyo
TECHNO: JURNAL PENELITIAN Vol 10, No 2 (2021): Techno Jurnal Penelitian
Publisher : Universitas Khairun

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33387/tjp.v10i2.2824

Abstract

In daily life, the interaction of the Osing people with their agricultural environment has resulted in various activities that have local wisdom values. All of these activities are still carried out in the midst of the rapid advancement of digital and online technology-based civilization. In addition, in their social life, the community is consistent in carrying out and preserving various traditional ritual traditions from their ancestral heritage. The research aims to make an inventory of various activities that have local wisdom values and record they carry out traditional conservation of the biological resources in their environment. The research used a qualitative descriptive method, while data collection was carried out through observation and interviews in Focus Group Discussions (FGD). The data analysis process uses cross-checking, summarizing, and synthesizing. The results showed that there were various activities of the Osing community that contained local wisdom values, including farming practices using the Javanese calendar (pranotomongso) and the history of making and placing garden lands that function as buffer areas for paddy fields to prevent landslides. ancestors of the Osing tribe. The division of tasks in farming activities in the rice fields carried out by men and women of the Osing tribe reflects a combination of activities that have aspects of local wisdom. Likewise, the use of straw as animal feed and natural organic fertilizers, as well as the use of a kettle, fishing tools, the use of hunting dogs to get wild animals, and skills in the process of making traditional tools. The Osing community carries out traditional ecosystem-based conservation to protect all plants that grow on their agricultural lands. In addition, in maintaining the sustainability of clean water springs (belik) along the banks of the Sobo and Gulung rivers, they do this by making a ban on cutting down trees that grow around the riverbanks and performing the rebo wekasan salvation ritual.
Struktur dan komunitas tanaman pekarangan di desa Jabon Mekar, Kecamatan Parung, Bogor Budi Prasetyo; Dede Setiadi; Eko B. Walujo
Journal of Tropical Ethnobiology Vol. 2 No. 2 (2005): July 2005
Publisher : The Ethnobiological Society of Indonesia

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Abstract

Jabon Mekar village is well-known as the central of fruit producing. Many kinds of fruit are planted and developed in this area. It is estimated as a buffer zone of Jakarta and subject of the urban development. The aim of the research is to study the community structure and vegetation profile of home-garden system in the village of jabon Mekar. The research was located at Jabon Mekar village, subdistrict of Parung, Bogor regency. The methods used for vegetation analysis were the quadrate method to find density, frequency, dominance, and important index value of plant species. The result of the research found 311 species of plants from 245 genus, 86 families and 36 cultivars. The plants were grouped into 6 categories i.e. the group of miscellaneous plants, ornamental plants, fruit plants, vegetable plants, tradionally medicinal plants, and food plants. The highest value of density of plant species for all group plants based on function found at the home-garden 400 m2 width types and then followed by home-garden of 1200 m2, 800 m2, and 2000 m2 width types. The all group of plants based on function in every types of widen home-garden have a tendency not to spread. The highest relative frequency was the group of fruit plants followed by the group of ornamental, miscellaneous, traditionally medicinal, vegetable and food plants. While the highest value of relative dominance is the group of miscellaneous plants, and then followed by ornamental plants, fruit plants, vegetable plants, tradionally medicinal plants, and food plants. The diversity of plant species at home-garden was at the high level. At the all of the widen homegarden type, the research noted that there were 57 species of fruit plants and dominated by Musa spp. It is also noted that 105 species of ornamental plants dominated by Acalypha sinensis, and in the 48 species of traditionally medicinal plants is dominated by _Ageratum houstonianum. While in the 15 species of vegetable plants is dominated by Gnaw& gaemon. And in the 7 rpecies of food plants is dominated Manihot eseulenta. Finally, in the 79 species of misceliadeous gro,ip of plants is dominated by Polytrias amaura.
Deskripsi Tingkah Laku Owa Jawa (Hylobates moloch Audebert) di Taman Margasatwa Ragunan Budi Prasetyo; Santi Amelia
Sainsmat : Jurnal Ilmiah Ilmu Pengetahuan Alam Vol 2, No 2 (2013): September
Publisher : Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Negeri Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35580/sainsmat228272013

Abstract

Keberadaan Hylobates moloch (Owa Jawa) endemik di hutan-hutan konservasi seperti Taman Nasional Ujung Kulon, Gunung Halimun, Gunung Gede Pangrango, Cagar Alam Gunung Simpang, dan Leuweung Sancang, dan menurut IUCN Redlist status konservasi Owa tersebut termasuk dalam kategori endangered (genting). Penelitian bertujuan untuk mempelajari tingkah laku dan karakteristik Owa Jawa di Karantina Taman Margasatwa Ragunan (TMR). Metode kualitatif yang digunakan dalam penelitian adalah melakukan pengamatan langsung terhadap tingkah laku Owa Jawa dengan cara: a) deskripsi, b) ad libitum, c) focal sampling, dan d) scan sampling. Aktivitas harian hidup Owa Jawa yang mencakup makan dan minum, bergerak, beristirahat, serta bersosialisasi di Karantina TMR pada dasarnya mirip dengan perilaku Owa di habitat alamiahnya serta di Pusat Penyelamatan dan Rehabilitasi Owa Jawa (Javan Gibbon Center), Taman Nasional Gunung Gede Pangrango. Adanya kemiripan aktivitas harian hidup Owa Jawa mengindikasikan bahwa sudah selayaknya tidak ada alasan yang sangat mendasar dalam tata cara pemeliharaan maupun perawatan Owa Jawa di karantina TMR untuk tidak berhasil, sebaliknya harus berhasil dan berkembang menjadi lebih banyak.Kata kunci: Owa Jawa, tingkah laku, Taman Margasatwa Ragunan
DETEKSI GEN tst ISOLAT Staphylococcus aureus MELALUI AMPLIFIKASI 23S rRNA ASAL SUSU KAMBING DAN SAPI PERAH Budi Prasetyo; Elizabeth Novi Kusumaningrum
Jurnal Kedokteran Hewan Vol 8, No 1 (2014): March
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (249.415 KB) | DOI: 10.21157/j.ked.hewan.v8i1.1266

Abstract

Penelitian ini bertujuan mendeteksi gen tst isolat Staphylococcus aureus (S. aureus) susu sapi perah dan susu kambing melalui amplifikasi gen 23S rRNA sebagai langkah awal pencegahan beberapa kasus keracunan susu. Metode penelitian meliputi reidentifikasi bakteri, preparasi deoxyribonucleic acid (DNA), amplifikasi gen 23S rRNA, amplifikasi gen tst, dan pengurutan DNA. Hasil reidentifikasi bakteri melalui pewarnaan Gram, uji katalase, uji koagulase, uji fermentasi MSA, uji VJA, dan uji VP adalah positif bakteri S. aureus. Hasil amplifikasi gen tst terhadap 3 isolat S. aureus menunjukkan hanya 2 isolat memberikan hasil positif. Hasil positif tersebut ditandai dengan munculnya fragmen DNA yang memiliki panjang spesifik (350 bp) sesuai dengan produk polymerase chain reaction (PCR) dari referensi dan database GeneBank. Disimpulkan bahwa dengan menggunakan metode PCR dapat terdeteksi adanya gen tst pada isolat S. aureus asal susu sapi perah dan susu kambing dari Bogor.
Pembuatan Eco-Enzyme di Kelurahan Pondok Cabe Ilir, Pamulang, Tangerang Selatan: Solusi Penanganan Sampah Organik pada Level Rumah Tangga Sri Utami; Elizabeth Novi Kusumaningrum; Yuni Tri Hewindati; Heny Kurniawati; Fawzi Rahmadiyan Zuhairi; Budi Prasetyo
I-Com: Indonesian Community Journal Vol 3 No 2 (2023): I-Com: Indonesian Community Journal (Juni 2023)
Publisher : Fakultas Sains Dan Teknologi, Universitas Raden Rahmat Malang

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (513.907 KB) | DOI: 10.33379/icom.v3i2.2413

Abstract

Produksi sampah rumah tangga terus mengalami peningkatan jumlah setiap tahunnya, dan sampah organik menempati proporsi terbesar dari total produksi sampah. Salah satu cara efektif dalam mengatasi permasalahan sampah rumah tangga yakni melalui pembuatan eco-enzyme. Kegiatan Pengabdian kepada Masyarakat ini bertujuan untuk mealukan pemberdayaan masyarakat di wilayah Pamulang, Tangerang Selatan Banten melalui praktik pelatihan pembuatan eco-enzyme. Metode pelaksanaan meliputi pembentukan struktur organisasi kelompok penggerak, pemberian penyuluhan tentang eco-enzyme, pelatihan dan pendampingan pembuatan eco-enzyme, monitoring pembuatan eco-enzyme, dan pendampingan panen eco-enzyme. Hasil kegiatan menunjukkan bahwa masyarakat memperoleh pengetahuan dan keterampilan tentang pemanfaatan sisa-sisa bahan sayuran dan buah-buahan yang sudah tidak dikonsumsi di lingkungan rumah tangganya untuk dijadikan eco-enzyme. Cairan ini dapat dimanfaatkan sebagai pembersih serba guna, pupuk, perbaikan kualitas udara, dan pemanfaatan lainnya di kehidupan sehari-hari.
PEMILIHAN JENIS PELARUT EKTRAKSI PADA PENGUJIAN POTENSI ANTIBIOTIK NEOMISIN KRIM Fuji Sachara; Budi Prasetyo
Jurnal Farmamedika (Pharmamedika Journal) Vol 9 No 1 (2024): Jurnal Farmamedika (Pharmamedica Journal)
Publisher : Sekolah Tinggi Teknologi Industri dan Farmasi Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.47219/ath.v9i1.292

Abstract

Neomycin krim merupakan obat antibiotik dalam bentuk campuran antara emulsi minyak dan air. Pada Neomycin krim perlu dilakukan pengujian secara mikrobiologi untuk mengukur efek senyawa terhadap pertumbuhan mikroba uji yang peka melalui proses ekstraksi menggunakan suatu pelarut agar zat aktif terpisahkan dari campurannya. Penelitian bertujuan untuk mengetahui pengaruh jenis pelarut (dietil eter, petroleum benzene, dan kloroform) terhadap kadar potensi antibiotik neomisin krim. Penelitian menggunakan metode eksperimental, terdiri atas tahapan ekstraksi (persiapan sampel) menggunakan metode maserasi melalui ektraksi cair-cair, dan tahapan penetapan kadar potensi antibiotik menggunakan metode lempeng-silinder. Hasil pengamatan ekstrak dari masing-masing pelarut menunjukkan bahwa ekstrak dari pelarut kloroform terpisah menjadi dua fase, yaitu fase terdispers (pelarut buffer) berwarna bening yang secara proposional berada pada bagian atas dan fase pendispers berada pada bagian bawah. Ekstrak petroleum benzene terpisah menjadi dua fase, yaitu fase terdispers berwarna putih yang secara proposional berada pada bagian bawah dan fase pendispers berada pada bagian atas, sedangkan pada ekstrak dietil eter terpisah menjadi dua fase namun tidak proposional, yaitu fase terdispers berwarna putih bening berada pada bagian bawah dengan porsi lebih banyak dibanding fase pendispers yang berada pada bagian atas. Hasil uji potensi menunjukkan pada ektrak kloroform diperoleh kadar potensi 97,15%, ekstrak dietil eter 80,88%, dan ekstrak petroleum benzene 45,78%. Dengan demikian, kadar potensi optimum diperoleh pada ekstraksi menggunakan pelarut kloroform.
Co-Authors Dede Setiadi Eko B. Walujo Eko Baroto Walujo Elizabeth Novi Kusumaningrum Ervizal A.M Zuhud Fawzi Rahmadiyan Zuhairi Fuji Sachara Heny Kurniawati Santi Amelia Sri Utami TATIK CHIKMAWATI Yuni Tri Hewindati